The technological landscape and applications of single-cell multi-omics

Integration of whole transcriptome spatial profiling with protein markers

(2023) Nir Ben-Chetrit et al.   NATURE BIOTECHNOLOGY

Dissecting cell identity via network inference and in silico gene perturbation

(2023) Kenji Kamimoto et al.   NATURE

Spatial epigenome–transcriptome co-profiling of mammalian tissues

(2023) Di Zhang et al.   NATURE

High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq

(2023) Yang Liu et al.   NATURE BIOTECHNOLOGY

Single-cell biological network inference using a heterogeneous graph transformer

(2023) Anjun Ma et al.   Nature Communications

Spatial-CUT&Tag: Spatially resolved chromatin modification profiling at the cellular level

(2022) Yanxiang Deng et al.   SCIENCE

SM-Omics is an automated platform for high-throughput spatial multi-omics

(2022) S. Vickovic et al.   Nature Communications

A blood atlas of COVID-19 defines hallmarks of disease severity and specificity

(2022) David J. Ahern et al.   CELL

Characterizing cellular heterogeneity in chromatin state with scCUT&Tag-pro

(2022) Bingjie Zhang et al.   NATURE BIOTECHNOLOGY

Multi-omics single-cell data integration and regulatory inference with graph-linked embedding

(2022) Zhi-Jie Cao et al.   NATURE BIOTECHNOLOGY

Deep Visual Proteomics defines single-cell identity and heterogeneity

(2022) Andreas Mund et al.   NATURE BIOTECHNOLOGY

NEAT-seq: simultaneous profiling of intra-nuclear proteins, chromatin accessibility and gene expression in single cells

(2022) Amy F. Chen et al.   NATURE METHODS

Single-cell atlases: shared and tissue-specific cell types across human organs

(2022) Rasa Elmentaite et al.   NATURE REVIEWS GENETICS

Into the multiverse: advances in single-cell multiomic profiling

(2022) Silvia Ogbeide et al.   TRENDS IN GENETICS

Spatial profiling of chromatin accessibility in mouse and human tissues

(2022) Yanxiang Deng et al.   NATURE

Single-cell antigen-specific landscape of CAR T infusion product identifies determinants of CD19-positive relapse in patients with ALL

(2022) Zhiliang Bai et al.   Science Advances

Spatial maps of T cell receptors and transcriptomes reveal distinct immune niches and interactions in the adaptive immune response

(2022) Sophia Liu et al.   IMMUNITY

Clonal lineage tracing reveals mechanisms skewing CD8+ T cell fate decisions in chronic infection

(2022) Moujtaba Y. Kasmani et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Scalable in situ single-cell profiling by electrophoretic capture of mRNA using EEL FISH

(2022) Lars E. Borm et al.   NATURE BIOTECHNOLOGY

Multi-omic single-cell velocity models epigenome–transcriptome interactions and improves cell fate prediction

(2022) Chen Li et al.   NATURE BIOTECHNOLOGY

High-plex imaging of RNA and proteins at subcellular resolution in fixed tissue by spatial molecular imaging

(2022) Shanshan He et al.   NATURE BIOTECHNOLOGY

MIRA: joint regulatory modeling of multimodal expression and chromatin accessibility in single cells

(2022) Allen W. Lynch et al.   NATURE METHODS

ISSAAC-seq enables sensitive and flexible multimodal profiling of chromatin accessibility and gene expression in single cells

(2022) Wei Xu et al.   NATURE METHODS

Multiomics of Colorectal Cancer Organoids Reveals Putative Mediators of Cancer Progression Resulting from SMAD4 Inactivation

(2022) Jelmer J. Dijkstra et al.   Journal of Proteome Research

A spatially resolved atlas of the human lung characterizes a gland-associated immune niche

(2022) Elo Madissoon et al.   NATURE GENETICS

Single-cell transcriptomics for the assessment of cardiac disease

(2022) Antonio M. A. Miranda et al.   Nature Reviews Cardiology

Sequencing of 53,831 diverse genomes from the NHLBI TOPMed Program

(2021) Daniel Taliun et al.   NATURE

Joint profiling of histone modifications and transcriptome in single cells from mouse brain

(2021) Chenxu Zhu et al.   NATURE METHODS

Single-cell multiomics sequencing reveals the functional regulatory landscape of early embryos

(2021) Yang Wang et al.   Nature Communications

Review of multi-omics data resources and integrative analysis for human brain disorders

(2021) Xianjun Dong et al.   Briefings in Functional Genomics

Single-cell CUT&Tag profiles histone modifications and transcription factors in complex tissues

(2021) Marek Bartosovic et al.   NATURE BIOTECHNOLOGY

Single-cell multi-omics analysis of the immune response in COVID-19

(2021) Emily Stephenson et al.   NATURE MEDICINE

Single-cell joint detection of chromatin occupancy and transcriptome enables higher-dimensional epigenomic reconstructions

(2021) Haiqing Xiong et al.   NATURE METHODS

Towards a Human Cell Atlas: Taking Notes from the Past

(2021) Rik G.H. Lindeboom et al.   TRENDS IN GENETICS

Simultaneous trimodal single-cell measurement of transcripts, epitopes, and chromatin accessibility using TEA-seq

(2021) Elliott Swanson et al.   eLife

Manifold alignment for heterogeneous single-cell multi-omics data integration using Pamona

(2021) Kai Cao et al.   BIOINFORMATICS

Deep cross-omics cycle attention model for joint analysis of single-cell multi-omics data

(2021) Chunman Zuo et al.   BIOINFORMATICS

Integrated analysis of multimodal single-cell data

(2021) Yuhan Hao et al.   CELL

Scalable, multimodal profiling of chromatin accessibility, gene expression and protein levels in single cells

(2021) Eleni P. Mimitou et al.   NATURE BIOTECHNOLOGY

SCITO-seq: single-cell combinatorial indexed cytometry sequencing

(2021) Byungjin Hwang et al.   NATURE METHODS

High-throughput and single-cell T cell receptor sequencing technologies

(2021) Joy A. Pai et al.   NATURE METHODS

A plate-based single-cell ATAC-seq workflow for fast and robust profiling of chromatin accessibility

(2021) Wei Xu et al.   Nature Protocols

High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer

(2021) Sarah E. Pierce et al.   Nature Communications

Spatial genomics enables multi-modal study of clonal heterogeneity in tissues

(2021) Tongtong Zhao et al.   NATURE

Chromatin Velocity reveals epigenetic dynamics by single-cell profiling of heterochromatin and euchromatin

(2021) Martina Tedesco et al.   NATURE BIOTECHNOLOGY

Single-cell profiling of proteins and chromatin accessibility using PHAGE-ATAC

(2021) Evgenij Fiskin et al.   NATURE BIOTECHNOLOGY

Lineage recording in human cerebral organoids

(2021) Zhisong He et al.   NATURE METHODS

Joint single-cell measurements of nuclear proteins and RNA in vivo

(2021) Hattie Chung et al.   NATURE METHODS

Spatial immunophenotypes predict response to anti-PD1 treatment and capture distinct paths of T cell evasion in triple negative breast cancer

(2021) Dora Hammerl et al.   Nature Communications

Single-cell genomic approaches for developing the next generation of immunotherapies

(2020) Ido Yofe et al.   NATURE MEDICINE

CellPhoneDB: inferring cell–cell communication from combined expression of multi-subunit ligand–receptor complexes

(2020) Mirjana Efremova et al.   Nature Protocols

Using Single-Cell and Spatial Transcriptomes to Understand Stem Cell Lineage Specification During Early Embryo Development

(2020) Guangdun Peng et al.   Annual Review of Genomics and Human Genetics

CiteFuse enables multi-modal analysis of CITE-seq data

(2020) Hani Jieun Kim et al.   BIOINFORMATICS

Single-cell RNA counting at allele and isoform resolution using Smart-seq3

(2020) Michael Hagemann-Jensen et al.   NATURE BIOTECHNOLOGY

Multiplex digital spatial profiling of proteins and RNA in fixed tissue

(2020) Christopher R. Merritt et al.   NATURE BIOTECHNOLOGY

Lineage tracing meets single-cell omics: opportunities and challenges

(2020) Daniel E. Wagner et al.   NATURE REVIEWS GENETICS

BREM-SC: a bayesian random effects mixture model for joint clustering single cell multi-omics data

(2020) Xinjun Wang et al.   NUCLEIC ACIDS RESEARCH

Integrative Methods and Practical Challenges for Single-Cell Multi-omics

(2020) Anjun Ma et al.   TRENDS IN BIOTECHNOLOGY

Unsupervised topological alignment for single-cell multi-omics integration

(2020) Kai Cao et al.   BIOINFORMATICS

Proteogenomics of Non-smoking Lung Cancer in East Asia Delineates Molecular Signatures of Pathogenesis and Progression

(2020) Yi-Ju Chen et al.   CELL

Coupled scRNA-Seq and Intracellular Protein Activity Reveal an Immunosuppressive Role of TREM2 in Cancer

(2020) Yonatan Katzenelenbogen et al.   CELL

Parallel bimodal single-cell sequencing of transcriptome and chromatin accessibility

(2020) Qiao Rui Xing et al.   GENOME RESEARCH

Expanded encyclopaedias of DNA elements in the human and mouse genomes

(2020) Jill E. Moore et al.   NATURE

Massively parallel single-cell mitochondrial DNA genotyping and chromatin profiling

(2020) Caleb A. Lareau et al.   NATURE BIOTECHNOLOGY

Chromatin Potential Identified by Shared Single-Cell Profiling of RNA and Chromatin

(2020) Sai Ma et al.   CELL

Single-cell multiomics: technologies and data analysis methods

(2020) Jeongwoo Lee et al.   EXPERIMENTAL AND MOLECULAR MEDICINE

Deep-joint-learning analysis model of single cell transcriptome and open chromatin accessibility data

(2020) Chunman Zuo et al.   BRIEFINGS IN BIOINFORMATICS

High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue

(2020) Yang Liu et al.   CELL

Next-Generation Lineage Tracing and Fate Mapping to Interrogate Development

(2020) Sadie VanHorn et al.   DEVELOPMENTAL CELL

Highly sensitive spatial transcriptomics at near-cellular resolution with Slide-seqV2

(2020) Robert R. Stickels et al.   NATURE BIOTECHNOLOGY

CPT1A Over-Expression Increases Reactive Oxygen Species in the Mitochondria and Promotes Antioxidant Defenses in Prostate Cancer

(2020) Molishree Joshi et al.   Cancers

Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells

(2019) Jan. P. Gerlach et al.   Scientific Reports

Deconvolution of single-cell multi-omics layers reveals regulatory heterogeneity

(2019) Longqi Liu et al.   Nature Communications

Slide-seq: A scalable technology for measuring genome-wide expression at high spatial resolution

(2019) Samuel G. Rodriques et al.   SCIENCE

Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH+

(2019) Chee-Huat Linus Eng et al.   NATURE

Unravelling Intratumoral Heterogeneity through High-Sensitivity Single-Cell Mutational Analysis and Parallel RNA Sequencing

(2019) Alba Rodriguez-Meira et al.   MOLECULAR CELL

Single-Cell Proteomics Reveal that Quantitative Changes in Co-expressed Lineage-Specific Transcription Factors Determine Cell Fate

(2019) Carmen G. Palii et al.   Cell Stem Cell

Epigenetic evolution and lineage histories of chronic lymphocytic leukaemia

(2019) Federico Gaiti et al.   NATURE

Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells

(2019) Eleni P. Mimitou et al.   NATURE METHODS

Comprehensive Integration of Single-Cell Data

(2019) Tim Stuart et al.   CELL

Single-Cell Multi-omic Integration Compares and Contrasts Features of Brain Cell Identity

(2019) Joshua D. Welch et al.   CELL

High-throughput single-cell ChIP-seq identifies heterogeneity of chromatin states in breast cancer

(2019) Kevin Grosselin et al.   NATURE GENETICS

RNase H–dependent PCR-enabled T-cell receptor sequencing for highly specific and efficient targeted sequencing of T-cell receptor mRNA for single-cell and repertoire analysis

(2019) Shuqiang Li et al.   Nature Protocols

T‐cell receptor and B‐cell receptor repertoire profiling in adaptive immunity

(2019) Anastasia Minervina et al.   TRANSPLANT INTERNATIONAL

Model-based understanding of single-cell CRISPR screening

(2019) Bin Duan et al.   Nature Communications

High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes

(2019) Mandeep Singh et al.   Nature Communications

CUT&Tag for efficient epigenomic profiling of small samples and single cells

(2019) Hatice S. Kaya-Okur et al.   Nature Communications

Integrating multi-omics longitudinal data to reconstruct networks underlying lung development

(2019) Jun Ding et al.   AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY

Spectrum: fast density-aware spectral clustering for single and multi-omic data

(2019) Christopher R John et al.   BIOINFORMATICS

A new era of long-read sequencing for cancer genomics

(2019) Yoshitaka Sakamoto et al.   JOURNAL OF HUMAN GENETICS

Metabolomic, transcriptomic and genetic integrative analysis reveals important roles of adenosine diphosphate in haemostasis and platelet activation in non‐small‐cell lung cancer

(2019) Long T. Hoang et al.   Molecular Oncology

Massively parallel single-cell chromatin landscapes of human immune cell development and intratumoral T cell exhaustion

(2019) Ansuman T. Satpathy et al.   NATURE BIOTECHNOLOGY

High-definition spatial transcriptomics for in situ tissue profiling

(2019) Sanja Vickovic et al.   NATURE METHODS

High-throughput sequencing of the transcriptome and chromatin accessibility in the same cell

(2019) Song Chen et al.   NATURE BIOTECHNOLOGY

TCR sequencing paired with massively parallel 3′ RNA-seq reveals clonotypic T cell signatures

(2019) Ang A. Tu et al.   NATURE IMMUNOLOGY

Probabilistic cell typing enables fine mapping of closely related cell types in situ

(2019) Xiaoyan Qian et al.   NATURE METHODS

An ultra high-throughput method for single-cell joint analysis of open chromatin and transcriptome

(2019) Chenxu Zhu et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Single-cell multiomic analysis identifies regulatory programs in mixed-phenotype acute leukemia

(2019) Jeffrey M. Granja et al.   NATURE BIOTECHNOLOGY

NicheNet: modeling intercellular communication by linking ligands to target genes

(2019) Robin Browaeys et al.   NATURE METHODS

Single-Cell Transcriptomics Meets Lineage Tracing

(2018) Lennart Kester et al.   Cell Stem Cell

Publisher Correction: Single-cell gene expression reveals a landscape of regulatory T cell phenotypes shaped by the TCR

(2018) David Zemmour et al.   NATURE IMMUNOLOGY

Transcript-indexed ATAC-seq for precision immune profiling

(2018) Ansuman T. Satpathy et al.   NATURE MEDICINE

Three-dimensional intact-tissue sequencing of single-cell transcriptional states

(2018) Xiao Wang et al.   SCIENCE

Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding

(2018) Alexander B. Rosenberg et al.   SCIENCE

scNMT-seq enables joint profiling of chromatin accessibility DNA methylation and transcription in single cells

(2018) Stephen J. Clark et al.   Nature Communications

Simultaneous Multiplexed Imaging of mRNA and Proteins with Subcellular Resolution in Breast Cancer Tissue Samples by Mass Cytometry

(2018) Daniel Schulz et al.   Cell Systems

Deep Profiling of Mouse Splenic Architecture with CODEX Multiplexed Imaging

(2018) Yury Goltsev et al.   CELL

Joint profiling of chromatin accessibility and gene expression in thousands of single cells

(2018) Junyue Cao et al.   SCIENCE

High-throughput chromatin accessibility profiling at single-cell resolution

(2018) Anja Mezger et al.   Nature Communications

Organoid Modeling of the Tumor Immune Microenvironment

(2018) James T. Neal et al.   CELL

Spatial organization of the somatosensory cortex revealed by osmFISH

(2018) Simone Codeluppi et al.   NATURE METHODS

Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region

(2018) Jeffrey R. Moffitt et al.   SCIENCE

Single-cell multiomics sequencing and analyses of human colorectal cancer

(2018) Shuhui Bian et al.   SCIENCE

Joint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity

(2018) Xingqi Chen et al.   Nature Communications

A rapid and robust method for single cell chromatin accessibility profiling

(2018) Xi Chen et al.   Nature Communications

SIDR: simultaneous isolation and parallel sequencing of genomic DNA and total RNA from single cells

(2017) Kyung Yeon Han et al.   GENOME RESEARCH

Multiplexed Engineering and Analysis of Combinatorial Enhancer Activity in Single Cells

(2017) Shiqi Xie et al.   MOLECULAR CELL

Discovering and linking public omics data sets using the Omics Discovery Index

(2017) Yasset Perez-Riverol et al.   NATURE BIOTECHNOLOGY

Multiplexed quantification of proteins and transcripts in single cells

(2017) Vanessa M Peterson et al.   NATURE BIOTECHNOLOGY

Simultaneous epitope and transcriptome measurement in single cells

(2017) Marlon Stoeckius et al.   NATURE METHODS

Sequencing thousands of single-cell genomes with combinatorial indexing

(2017) Sarah A Vitak et al.   NATURE METHODS

Pooled CRISPR screening with single-cell transcriptome readout

(2017) Paul Datlinger et al.   NATURE METHODS

Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput

(2017) Todd M Gierahn et al.   NATURE METHODS

Spatial transcriptomic analysis of cryosectioned tissue samples with Geo-seq

(2017) Jun Chen et al.   Nature Protocols

Building a lineage from single cells: genetic techniques for cell lineage tracking

(2017) Mollie B. Woodworth et al.   NATURE REVIEWS GENETICS

The human protein atlas: A spatial map of the human proteome

(2017) Peter J. Thul et al.   PROTEIN SCIENCE

Massively parallel digital transcriptional profiling of single cells

(2017) Grace X. Y. Zheng et al.   Nature Communications

The Human Cell Atlas

(2017) Aviv Regev et al.   eLife

Simultaneous measurement of chromatin accessibility, DNA methylation, and nucleosome phasing in single cells

(2017) Sebastian Pott   eLife

An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites

(2017) Peter J Skene et al.   eLife

Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with Single-Cell RNA-Seq

(2016) Diego Adhemar Jaitin et al.   CELL

Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens

(2016) Atray Dixit et al.   CELL

Single-cell triple omics sequencing reveals genetic, epigenetic and transcriptomic heterogeneity in hepatocellular carcinomas

(2016) Yu Hou et al.   CELL RESEARCH

Digital Quantification of Proteins and mRNA in Single Mammalian Cells

(2016) Cem Albayrak et al.   MOLECULAR CELL

Modulation of long noncoding RNAs by risk SNPs underlying genetic predispositions to prostate cancer

(2016) Haiyang Guo et al.   NATURE GENETICS

Highly multiplexed simultaneous detection of RNAs and proteins in single cells

(2016) Andreas P Frei et al.   NATURE METHODS

Nanoscale imaging of RNA with expansion microscopy

(2016) Fei Chen et al.   NATURE METHODS

Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity

(2016) Christof Angermueller et al.   NATURE METHODS

Visualization and analysis of gene expression in tissue sections by spatial transcriptomics

(2016) P. L. Stahl et al.   SCIENCE

Simultaneous Multiplexed Measurement of RNA and Proteins in Single Cells

(2016) Spyros Darmanis et al.   Cell Reports

Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines

(2015) Eric Kowarz et al.   Biotechnology Journal

Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells

(2015) Allon M. Klein et al.   CELL

Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets

(2015) Evan Z. Macosko et al.   CELL

Single-cell chromatin accessibility reveals principles of regulatory variation

(2015) Jason D. Buenrostro et al.   NATURE

Integrated genome and transcriptome sequencing of the same cell

(2015) Siddharth S Dey et al.   NATURE BIOTECHNOLOGY

G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

(2015) Iain C Macaulay et al.   NATURE METHODS

Profiling DNA methylome landscapes of mammalian cells with single-cell reduced-representation bisulfite sequencing

(2015) Hongshan Guo et al.   Nature Protocols

Spatially resolved, highly multiplexed RNA profiling in single cells

(2015) K. H. Chen et al.   SCIENCE

Multiplex single-cell profiling of chromatin accessibility by combinatorial cellular indexing

(2015) D. A. Cusanovich et al.   SCIENCE

The Genotype-Tissue Expression (GTEx) pilot analysis: Multitissue gene regulation in humans

(2015) et al.   SCIENCE

Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines

(2015) Eric Kowarz et al.   Biotechnology Journal

Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity

(2014) Sébastien A Smallwood et al.   NATURE METHODS

Improved vectors and genome-wide libraries for CRISPR screening

(2014) Neville E Sanjana et al.   NATURE METHODS

Single-cell in situ RNA profiling by sequential hybridization

(2014) Eric Lubeck et al.   NATURE METHODS

Full-length RNA-seq from single cells using Smart-seq2

(2014) Simone Picelli et al.   Nature Protocols

Highly Multiplexed Subcellular RNA Sequencing in Situ

(2014) J. H. Lee et al.   SCIENCE

Massively Parallel Single-Cell RNA-Seq for Marker-Free Decomposition of Tissues into Cell Types

(2014) D. A. Jaitin et al.   SCIENCE

Co-detection and sequencing of genes and transcripts from the same single cells facilitated by a microfluidics platform

(2014) Lin Han et al.   Scientific Reports

The Cancer Genome Atlas Pan-Cancer analysis project

(2013) John N Weinstein et al.   NATURE GENETICS

In situ sequencing for RNA analysis in preserved tissue and cells

(2013) Rongqin Ke et al.   NATURE METHODS

Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position

(2013) Jason D Buenrostro et al.   NATURE METHODS

Method of the Year 2013

(2013)   NATURE METHODS

Quantitative PCR Analysis of DNA, RNAs, and Proteins in the Same Single Cell

(2012) A. Stahlberg et al.   CLINICAL CHEMISTRY

The genomic and transcriptomic architecture of 2,000 breast tumours reveals novel subgroups

(2012) Christina Curtis et al.   NATURE

An integrated encyclopedia of DNA elements in the human genome

(2012)   NATURE

Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells

(2012) Daniel Ramsköld et al.   NATURE BIOTECHNOLOGY

Highly multiplexed and strand-specific single-cell RNA 5′ end sequencing

(2012) Saiful Islam et al.   Nature Protocols

DNA Methylation and Its Basic Function

(2012) Lisa D Moore et al.   NEUROPSYCHOPHARMACOLOGY

CEL-Seq: Single-Cell RNA-Seq by Multiplexed Linear Amplification

(2012) Tamar Hashimshony et al.   Cell Reports

RNAscope

(2011) Fay Wang et al.   JOURNAL OF MOLECULAR DIAGNOSTICS

mRNA-Seq whole-transcriptome analysis of a single cell

(2009) Fuchou Tang et al.   NATURE METHODS