RNase H–dependent PCR-enabled T-cell receptor sequencing for highly specific and efficient targeted sequencing of T-cell receptor mRNA for single-cell and repertoire analysis
Published 2019 View Full Article
- Home
- Publications
- Publication Search
- Publication Details
Title
RNase H–dependent PCR-enabled T-cell receptor sequencing for highly specific and efficient targeted sequencing of T-cell receptor mRNA for single-cell and repertoire analysis
Authors
Keywords
-
Journal
Nature Protocols
Volume 14, Issue 8, Pages 2571-2594
Publisher
Springer Science and Business Media LLC
Online
2019-07-25
DOI
10.1038/s41596-019-0195-x
References
Ask authors/readers for more resources
Related references
Note: Only part of the references are listed.- Unique, dual-indexed sequencing adapters with UMIs effectively eliminate index cross-talk and significantly improve sensitivity of massively parallel sequencing
- (2018) Laura E. MacConaill et al. BMC GENOMICS
- Single-Cell Map of Diverse Immune Phenotypes in the Breast Tumor Microenvironment
- (2018) Elham Azizi et al. CELL
- Immune Repertoire Sequencing Using Molecular Identifiers Enables Accurate Clonality Discovery and Clone Size Quantification
- (2018) Ke-Yue Ma et al. Frontiers in Immunology
- A cloning and expression system to probe T cell receptor specificity and assess functional avidity to neoantigens
- (2018) Zhuting Hu et al. BLOOD
- Organoid Modeling of the Tumor Immune Microenvironment
- (2018) James T. Neal et al. CELL
- Neoantigen vaccine generates intratumoral T cell responses in phase Ib glioblastoma trial
- (2018) Derin B. Keskin et al. NATURE
- Quantifiable predictive features define epitope-specific T cell receptor repertoires
- (2017) Pradyot Dash et al. NATURE
- Targeted reconstruction of T cell receptor sequence from single cell RNA-seq links CDR3 length to T cell differentiation state
- (2017) Shaked Afik et al. NUCLEIC ACIDS RESEARCH
- T cell receptor sequencing of early-stage breast cancer tumors identifies altered clonal structure of the T cell repertoire
- (2017) John F. Beausang et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- A rapid method to verify single-cell deposition setup for cell sorters
- (2016) Olivia Roos Rodrigues et al. CYTOMETRY PART A
- Linking the T cell receptor to the single cell transcriptome in antigen-specific human T cells
- (2016) Auda A Eltahla et al. IMMUNOLOGY AND CELL BIOLOGY
- Landscape of tumor-infiltrating T cell repertoire of human cancers
- (2016) Bo Li et al. NATURE GENETICS
- T cell fate and clonality inference from single-cell transcriptomes
- (2016) Michael J T Stubbington et al. NATURE METHODS
- High-quality full-length immunoglobulin profiling with unique molecular barcoding
- (2016) M A Turchaninova et al. Nature Protocols
- Single-cell TCRseq: paired recovery of entire T-cell alpha and beta chain transcripts in T-cell receptors from single-cell RNAseq
- (2016) David Redmond et al. Genome Medicine
- Intratumoral expression levels of PD-L1, GZMA, and HLA-A along with oligoclonal T cell expansion associate with response to nivolumab in metastatic melanoma
- (2016) Hiroyuki Inoue et al. OncoImmunology
- Library construction for next-generation sequencing: Overviews and challenges
- (2016) Steven R. Head et al. BIOTECHNIQUES
- Quantitative Profiling of Immune Repertoires for Minor Lymphocyte Counts Using Unique Molecular Identifiers
- (2015) Evgeny S. Egorov et al. JOURNAL OF IMMUNOLOGY
- MiXCR: software for comprehensive adaptive immunity profiling
- (2015) Dmitriy A Bolotin et al. NATURE METHODS
- High-throughput pairing of T cell receptor α and β sequences
- (2015) Bryan Howie et al. Science Translational Medicine
- Peripheral T cell receptor diversity is associated with clinical outcomes following ipilimumab treatment in metastatic melanoma
- (2015) Michael A. Postow et al. Journal for ImmunoTherapy of Cancer
- Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling
- (2014) O. V. Britanova et al. JOURNAL OF IMMUNOLOGY
- PD-1 blockade induces responses by inhibiting adaptive immune resistance
- (2014) Paul C. Tumeh et al. NATURE
- Linking T-cell receptor sequence to functional phenotype at the single-cell level
- (2014) Arnold Han et al. NATURE BIOTECHNOLOGY
- Towards error-free profiling of immune repertoires
- (2014) Mikhail Shugay et al. NATURE METHODS
- Full-length RNA-seq from single cells using Smart-seq2
- (2014) Simone Picelli et al. Nature Protocols
- Improved Survival with T Cell Clonotype Stability After Anti-CTLA-4 Treatment in Cancer Patients
- (2014) E. Cha et al. Science Translational Medicine
- Distinct immunological mechanisms of CTLA-4 and PD-1 blockade revealed by analyzing TCR usage in blood lymphocytes
- (2014) Lidia Robert et al. OncoImmunology
- RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers
- (2011) Joseph R Dobosy et al. BMC BIOTECHNOLOGY
- Counting absolute numbers of molecules using unique molecular identifiers
- (2011) Teemu Kivioja et al. NATURE METHODS
- Double indexing overcomes inaccuracies in multiplex sequencing on the Illumina platform
- (2011) Martin Kircher et al. NUCLEIC ACIDS RESEARCH
- Systematic Comparison of Three Methods for Fragmentation of Long-Range PCR Products for Next Generation Sequencing
- (2011) Ellen Knierim et al. PLoS One
- Deep Sequencing of the Human TCR and TCR Repertoires Suggests that TCR Rearranges After and T Cell Commitment
- (2011) A. M. Sherwood et al. Science Translational Medicine
- Paired analysis of TCRα and TCRβ chains at the single-cell level in mice
- (2010) Pradyot Dash et al. JOURNAL OF CLINICAL INVESTIGATION
- High throughput sequencing reveals a complex pattern of dynamic interrelationships among human T cell subsets
- (2010) C. Wang et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Overlap and Effective Size of the Human CD8+ T Cell Receptor Repertoire
- (2010) H. S. Robins et al. Science Translational Medicine
- BLAST+: architecture and applications
- (2009) Christiam Camacho et al. BMC BIOINFORMATICS
- Comprehensive assessment of the TCRBV repertoire in small T-cell samples by means of an improved and convenient multiplex PCR method
- (2009) Sara Mariani et al. EXPERIMENTAL HEMATOLOGY
- Measurement and Clinical Monitoring of Human Lymphocyte Clonality by Massively Parallel V-D-J Pyrosequencing
- (2009) S. D. Boyd et al. Science Translational Medicine
- A large genome center's improvements to the Illumina sequencing system
- (2008) Michael A Quail et al. NATURE METHODS
Add your recorded webinar
Do you already have a recorded webinar? Grow your audience and get more views by easily listing your recording on Peeref.
Upload NowAsk a Question. Answer a Question.
Quickly pose questions to the entire community. Debate answers and get clarity on the most important issues facing researchers.
Get Started