Recent developments of genetically encoded optical sensors for cell biology

Synchronous Bioimaging of Intracellular pH and Chloride Based on LSS Fluorescent Protein

(2016) Jose M. Paredes et al.   ACS Chemical Biology

The biosensor toolbox for plant developmental biology

(2016) Veli V Uslu et al.   CURRENT OPINION IN PLANT BIOLOGY

Designing BRET-based conformational biosensors for G protein-coupled receptors

(2016) Rory Sleno et al.   METHODS

Design and construction of conformational biosensors to monitor ion channel activation: A prototype FlAsH/BRET-approach to Kir3 channels

(2016) Derek N. Robertson et al.   METHODS

Apollo-NADP+: a spectrally tunable family of genetically encoded sensors for NADP+

(2016) William D Cameron et al.   NATURE METHODS

Biosensor reveals multiple sources for mitochondrial NAD+

(2016) X. A. Cambronne et al.   SCIENCE

A Genetically Encoded FRET Probe to Detect Intranucleosomal Histone H3K9 or H3K14 Acetylation Using BRD4, a BET Family Member

(2015) Shiho Nakaoka et al.   ACS Chemical Biology

Rapid, randomized development of genetically encoded FRET sensors for small molecules

(2015) Estevão Aun Peroza et al.   ANALYST

Apoptosis induction-related cytosolic calcium responses revealed by the dual FRET imaging of calcium signals and caspase-3 activation in a single cell

(2015) Akitoshi Miyamoto et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Fluorescent ratiometric pH indicator SypHer2: Applications in neuroscience and regenerative biology

(2015) Mikhail E. Matlashov et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Intracellular pH imaging in cancer cells in vitro and tumors in vivo using the new genetically encoded sensor SypHer2

(2015) Marina V. Shirmanova et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Highly Stabletrans-Cyclooctene Amino Acids for Live-Cell Labeling

(2015) Jan-Erik Hoffmann et al.   CHEMISTRY-A EUROPEAN JOURNAL

Novel uses of fluorescent proteins

(2015) Alexander S Mishin et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Emerging fluorescent protein technologies

(2015) Jhon Ralph Enterina et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Homo-FRET Based Biosensors and Their Application to Multiplexed Imaging of Signalling Events in Live Cells

(2015) Sean Warren et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Genetically-encoded FRET-based sensors for monitoring Zn2+ in living cells

(2015) Anne M. Hessels et al.   Metallomics

Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange

(2015) Yidan Ding et al.   NATURE METHODS

Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging

(2015) Shai Berlin et al.   NATURE METHODS

Green-to-Red Photoconversion of GCaMP

(2015) Minrong Ai et al.   PLoS One

Labeling of active neural circuits in vivo with designed calcium integrators

(2015) B. F. Fosque et al.   SCIENCE

Comparison of genetically encoded calcium indicators for monitoring action potentials in mammalian brain by two-photon excitation fluorescence microscopy

(2015) Borbala Podor et al.   Neurophotonics

Intramolecular ex vivo Fluorescence Resonance Energy Transfer (FRET) of Dihydropyridine Receptor (DHPR) β1a Subunit Reveals Conformational Change Induced by RYR1 in Mouse Skeletal Myotubes

(2015) Dipankar Bhattacharya et al.   PLoS One

Lack of Neutrophil Elastase Reduces Inflammation, Mucus Hypersecretion, and Emphysema, but Not Mucus Obstruction, in Mice with Cystic Fibrosis–like Lung Disease

(2014) Stefanie Gehrig et al.   AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE

Fluorescent-protein-based probes: general principles and practices

(2014) Hui-wang Ai   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

In Vivo Imaging of Mouse Tumors by a Lipidated Cathepsin S Substrate

(2014) Hai-Yu Hu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Minimal Tags for Rapid Dual-Color Live-Cell Labeling and Super-Resolution Microscopy

(2014) Ivana Nikić et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Photocontrollable Fluorescent Proteins for Superresolution Imaging

(2014) Daria M. Shcherbakova et al.   Annual Review of Biophysics

Genetically Encoded Fluorescent Biosensors for Live-Cell Visualization of Protein Phosphorylation

(2014) Laurel Oldach et al.   CHEMISTRY & BIOLOGY

pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis

(2014) Yi Shen et al.   JOURNAL OF CELL BIOLOGY

Bioluminescent sensor proteins for point-of-care therapeutic drug monitoring

(2014) Rudolf Griss et al.   Nature Chemical Biology

Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes

(2014) Thomas Thestrup et al.   NATURE METHODS

Rational design of a high-affinity, fast, red calcium indicator R-CaMP2

(2014) Masatoshi Inoue et al.   NATURE METHODS

Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for Pyruvate

(2014) Alejandro San Martín et al.   PLoS One

Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide

(2014) Yulia G. Ermakova et al.   Nature Communications

Robust Red FRET Sensors Using Self-Associating Fluorescent Domains

(2013) Laurens H. Lindenburg et al.   ACS Chemical Biology

Improved Orange and Red Ca2+ Indicators and Photophysical Considerations for Optogenetic Applications

(2013) Jiahui Wu et al.   ACS Chemical Neuroscience

In Vivo Fluorescent Adenosine 5′-Triphosphate (ATP) Imaging of Drosophila melanogaster and Caenorhabditis elegans by Using a Genetically Encoded Fluorescent ATP Biosensor Optimized for Low Temperatures

(2013) Taiichi Tsuyama et al.   ANALYTICAL CHEMISTRY

Sensing Acetylcholine and Anticholinesterase Compounds

(2013) Alberto Schena et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Quantitative monitoring of 2-oxoglutarate in Escherichia coli cells by a fluorescence resonance energy transfer-based biosensor

(2013) Chang Zhang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Genetically encoded fluorescent indicator for imaging NAD+/NADH ratio changes in different cellular compartments

(2013) Dmitry S. Bilan et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Genetically encoded FRET-based biosensor for imaging MMP-9 activity

(2013) Michal Stawarski et al.   BIOMATERIALS

Genetically encoded FRET-based nanosensor for in vivo measurement of leucine

(2013) Mohd Mohsin et al.   BIOSENSORS & BIOELECTRONICS

Real-Time Visualization of Neuronal Activity during Perception

(2013) Akira Muto et al.   CURRENT BIOLOGY

Genetically Encoded Fluorescent Probe for the Selective Detection of Peroxynitrite

(2013) Zhi-jie Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ultrasensitive fluorescent proteins for imaging neuronal activity

(2013) Tsai-Wen Chen et al.   NATURE

Engineering of weak helper interactions for high-efficiency FRET probes

(2013) Raik Grünberg et al.   NATURE METHODS

A Genetically Encoded FRET Lactate Sensor and Its Use To Detect the Warburg Effect in Single Cancer Cells

(2013) Alejandro San Martín et al.   PLoS One

A RhoC Biosensor Reveals Differences in the Activation Kinetics of RhoA and RhoC in Migrating Cells

(2013) Jon S. Zawistowski et al.   PLoS One

A Versatile Toolkit to Produce Sensitive FRET Biosensors to Visualize Signaling in Time and Space

(2013) R. D. Fritz et al.   Science Signaling

Illumination of the Spatial Order of Intracellular pH by Genetically Encoded pH-Sensitive Sensors

(2013) Mojca Benčina   SENSORS

A New Protein-Protein Interaction Sensor Based on Tripartite Split-GFP Association

(2013) Stéphanie Cabantous et al.   Scientific Reports

HyPer-3: A Genetically Encoded H2O2 Probe with Improved Performance for Ratiometric and Fluorescence Lifetime Imaging

(2012) Dmitry S. Bilan et al.   ACS Chemical Biology

Amino Acids for Diels-Alder Reactions in Living Cells

(2012) Tilman Plass et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Bimolecular fluorescence complementation (BiFC): A 5-year update and future perspectives

(2012) Yutaka Kodama et al.   BIOTECHNIQUES

D2-like dopamine and β-adrenergic receptors form a signaling complex that integrates Gs- and Gi-mediated regulation of adenylyl cyclase

(2012) R. Victor Rebois et al.   CELLULAR SIGNALLING

Live-Cell Studies of p300/CBP Histone Acetyltransferase Activity and Inhibition

(2012) Beverley M. Dancy et al.   CHEMBIOCHEM

A Fluorogenic Red Fluorescent Protein Heterodimer

(2012) Spencer C. Alford et al.   CHEMISTRY & BIOLOGY

A Semisynthetic Fluorescent Sensor Protein for Glutamate

(2012) Matthias A. Brun et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Highlightable Ca2+ Indicators for Live Cell Imaging

(2012) Hiofan Hoi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Reaction-Based Genetically Encoded Fluorescent Hydrogen Sulfide Sensors

(2012) Si Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Fluorescent Sensor for GABA and Synthetic GABAB Receptor Ligands

(2012) Anastasiya Masharina et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Minimum set of mutations needed to optimize cyan fluorescent proteins for live cell imaging

(2012) Marie Erard et al.   Molecular BioSystems

Improving FRET dynamic range with bright green and red fluorescent proteins

(2012) Amy J Lam et al.   NATURE METHODS

pHTomato, a red, genetically encoded indicator that enables multiplex interrogation of synaptic activity

(2012) Yulong Li et al.   NATURE NEUROSCIENCE

Visualization of Glutamine Transporter Activities in Living Cells Using Genetically Encoded Glutamine Sensors

(2012) Katrin Gruenwald et al.   PLoS One

pHlash: A New Genetically Encoded and Ratiometric Luminescence Sensor of Intracellular pH

(2012) Yunfei Zhang et al.   PLoS One

Genetically Encoded Green Fluorescent Ca2+ Indicators with Improved Detectability for Neuronal Ca2+ Signals

(2012) Masamichi Ohkura et al.   PLoS One

Target-Activated Prodrugs (TAPs) for the Autoregulated Inhibition of MMP12

(2012) Amanda Cobos-Correa et al.   ACS Medicinal Chemistry Letters

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%

(2012) Joachim Goedhart et al.   Nature Communications

Dimerization-Dependent Green and Yellow Fluorescent Proteins

(2012) Spencer C. Alford et al.   ACS Synthetic Biology

Rapid Development of Genetically Encoded FRET Reporters

(2011) Alen Piljić et al.   ACS Chemical Biology

Sequential bioluminescence resonance energy transfer–fluorescence resonance energy transfer-based ratiometric protease assays with fusion proteins of firefly luciferase and red fluorescent protein

(2011) Bruce R. Branchini et al.   ANALYTICAL BIOCHEMISTRY

Development of Probes for Cellular Functions Using Fluorescent Proteins and Fluorescence Resonance Energy Transfer

(2011) Atsushi Miyawaki   Annual Review of Biochemistry

Spatiotemporal Regulation of Small GTPases as Revealed by Probes Based on the Principle of Förster Resonance Energy Transfer (FRET): Implications for Signaling and Pharmacology

(2011) Etsuko Kiyokawa et al.   Annual Review of Pharmacology and Toxicology

A Fluorescent Reporter of AMPK Activity and Cellular Energy Stress

(2011) Peiling Tsou et al.   Cell Metabolism

Real-Time Imaging of Histone H4K12–Specific Acetylation Determines the Modes of Action of Histone Deacetylase and Bromodomain Inhibitors

(2011) Tamaki Ito et al.   CHEMISTRY & BIOLOGY

Dynamic Regulation of the Mitochondrial Proton Gradient during Cytosolic Calcium Elevations

(2011) Damon Poburko et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Semisynthesis of Fluorescent Metabolite Sensors on Cell Surfaces

(2011) Matthias A. Brun et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetically Encoded 1,2-Aminothiols Facilitate Rapid and Site-Specific Protein Labeling via a Bio-orthogonal Cyanobenzothiazole Condensation

(2011) Duy P. Nguyen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Imaging Intracellular pH in Live Cells with a Genetically Encoded Red Fluorescent Protein Sensor

(2011) Mathew Tantama et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Development of an optimized backbone of FRET biosensors for kinases and GTPases

(2011) Naoki Komatsu et al.   MOLECULAR BIOLOGY OF THE CELL

CODA-RET reveals functional selectivity as a result of GPCR heteromerization

(2011) Eneko Urizar et al.   Nature Chemical Biology

Measuring steady-state and dynamic endoplasmic reticulum and Golgi Zn2+ with genetically encoded sensors

(2011) Y. Qin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

PI3K/Akt signaling requires spatial compartmentalization in plasma membrane microdomains

(2011) X. Gao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An Expanded Palette of Genetically Encoded Ca2+ Indicators

(2011) Y. Zhao et al.   SCIENCE

Reversible Dimerization ofAequorea victoriaFluorescent Proteins Increases the Dynamic Range of FRET-Based Indicators

(2010) Ippei Kotera et al.   ACS Chemical Biology

Site-Specific, Orthogonal Labeling of Proteins in Intact Cells with Two Small Biarsenical Fluorophores

(2010) Alexander Zürn et al.   BIOCONJUGATE CHEMISTRY

A genetically encoded sensor for H2O2 with expanded dynamic range

(2010) Kseniya N. Markvicheva et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Multiplexing of Multicolor Bioluminescence Resonance Energy Transfer

(2010) Billy Breton et al.   BIOPHYSICAL JOURNAL

Visualizing Tyrosine Kinase Activity with Bipartite Tetracysteine Display

(2010) Sarmistha Ray-Saha et al.   CHEMBIOCHEM

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

(2010) Heinz Neumann et al.   NATURE

Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano

(2010) Kazuki Horikawa et al.   NATURE METHODS

Simultaneous intracellular chloride and pH measurements using a GFP-based sensor

(2010) Daniele Arosio et al.   NATURE METHODS

Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues

(2010) Dmitriy M. Chudakov et al.   PHYSIOLOGICAL REVIEWS

H+-Independent Glutamine Transport in Plant Root Tips

(2010) Huaiyu Yang et al.   PLoS One

Auto-Luminescent Genetically-Encoded Ratiometric Indicator for Real-Time Ca2+ Imaging at the Single Cell Level

(2010) Kenta Saito et al.   PLoS One

Visualization of JNK activity dynamics with a genetically encoded fluorescent biosensor

(2010) M. Fosbrink et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A novel BRET-based genetically encoded biosensor for functional imaging of hypoxia

(2009) Pablo Iglesias et al.   BIOSENSORS & BIOELECTRONICS

Visualizing and quantifying the differential cleavages of the eukaryotic translation initiation factors eIF4GI and eIF4GII in the enterovirus-infected cell

(2009) Yueh-Ying Hsu et al.   BIOTECHNOLOGY AND BIOENGINEERING

BRET3: a red-shifted bioluminescence resonance energy transfer (BRET)-based integrated platform for imaging protein-protein interactions from single live cells and living animals

(2009) Abhijit De et al.   FASEB JOURNAL

Genetically Encoded Sensors to Elucidate Spatial Distribution of Cellular Zinc

(2009) Philip J. Dittmer et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Red Fluorescent Protein pH Biosensor to Detect Concentrative Nucleoside Transport

(2009) Danielle E. Johnson et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Covalent Protein Labeling Based on Noncatalytic β-Lactamase and a Designed FRET Substrate

(2009) Shin Mizukami et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biarsenical Labeling of Vesicular Stomatitis Virus Encoding Tetracysteine-Tagged M Protein Allows Dynamic Imaging of M Protein and Virus Uncoating in Infected Cells

(2009) S. C. Das et al.   JOURNAL OF VIROLOGY

Genetically encoded FRET sensors to monitor intracellular Zn2+ homeostasis

(2009) Jan L Vinkenborg et al.   NATURE METHODS

Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators

(2009) Lin Tian et al.   NATURE METHODS

Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators

(2009) H. Imamura et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

Simultaneous Recording of Multiple Cellular Events by FRET

(2008) Alen Piljic et al.   ACS Chemical Biology

GLUT1 and GLUT9 as major contributors to glucose influx in HepG2 cells identified by a high sensitivity intramolecular FRET glucose sensor

(2008) Hitomi Takanaga et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Multiplexed FRET to Image Multiple Signaling Events in Live Cells

(2008) David M. Grant et al.   BIOPHYSICAL JOURNAL

Contribution of Fluorophores to Protein Kinase C FRET Probe Performance

(2008) Christiane A. Jost et al.   CHEMBIOCHEM

An Engineered Protein Tag for Multiprotein Labeling in Living Cells

(2008) Arnaud Gautier et al.   CHEMISTRY & BIOLOGY

Dopamine D2 receptors form higher order oligomers at physiological expression levels

(2008) Wen Guo et al.   EMBO JOURNAL

Chemical calcium indicators

(2008) R. Madelaine Paredes et al.   METHODS

Fluorescence Resonance Energy Transfer Analysis of  2a-Adrenergic Receptor Activation Reveals Distinct Agonist-Specific Conformational Changes

(2008) A. Zurn et al.   MOLECULAR PHARMACOLOGY

Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors

(2008) Hui-wang Ai et al.   NATURE METHODS

Detection of heteromerization of more than two proteins by sequential BRET-FRET

(2008) Paulina Carriba et al.   NATURE METHODS

Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins

(2008) Stephen R Adams et al.   Nature Protocols

Recombineering-mediated tagging of Drosophila genomic constructs for in vivo localization and acute protein inactivation

(2008) Koen J. T. Venken et al.   NUCLEIC ACIDS RESEARCH

Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors

(2008) M. Ouyang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A genetically encoded fluorescent sensor of ERK activity

(2008) C. D. Harvey et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Optical measurement of synaptic glutamate spillover and reuptake by linker optimized glutamate-sensitive fluorescent reporters

(2008) S. A. Hires et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Selective Chemical Labeling of Proteins with Small Fluorescent Molecules Based on Metal-Chelation Methodology

(2008) Nobuaki Soh   SENSORS