Article
Chemistry, Analytical
Xiaqing Zhu, Shuren Feng, Zhongyi Jiang, Huayue Zhang, Yanyan Wang, Haitao Yang, Zefang Wang
Summary: A rapid and sensitive biosensor for detecting biotin based on a novel fluorescent protein smURFP was developed in this study. By optimizing the binding of biotin to smURFP, the sensitivity of the biosensor was increased, achieving a wide linear range in both chicken and human serum. The limit of detection for biotin in human serum was as low as 0.4 nM, representing the lowest reported LOD for a biotin biosensor.
ANALYTICA CHIMICA ACTA
(2021)
Article
Nanoscience & Nanotechnology
Kandasamy Sasikumar, Ramar Rajamanikandan, Heongkyu Ju
Summary: We developed a fluorescence sensor using carbon dots (CDs) to detect bilirubin in human urine. The CDs showed spherical shape with a mean particle size of 2.6 nm and emitted fluorescence at 511 nm when excited at 435 nm. A good spectral match between bilirubin absorbance and CDs excitation allowed the sensor to selectively detect bilirubin. The sensor displayed a linear response to bilirubin concentration in a wide range of 3.5-45.5 mM with a low limit of detection and high recovery rate in clinical specimens.
JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES
(2023)
Article
Chemistry, Physical
Prabha Soundharraj, Durgalakshmi Dhinasekaran, Prakasarao Aruna, Singaravelu Ganesan
Summary: This study demonstrated the efficiency of silica-silver colloidal nanoparticles for optical biosensor applications, showing unique fluorescence properties in biomolecular interactions, good sensing efficacy for molecules like glucose, and validation of efficient glucose sensing in human urine and serum.
SURFACES AND INTERFACES
(2021)
Review
Biochemistry & Molecular Biology
Beatriz A. Rodas-Junco, Graciela E. Racagni-Di-Palma, Michel Canul-Chan, Javier Usorach, S. M. Teresa Hernandez-Sotomayor
Summary: Plants have developed mechanisms to process extracellular signals through transient molecules known as second messengers, which can modulate physiological responses and gene expression under stress conditions. Recent research has focused on the molecular mechanisms of lipid second messengers in response to osmotic stress, highlighting their ability to recruit target proteins to the membrane and affect protein function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Shijun Chen, Xiaodong Ma, Lin Wang, Yuanyuan Wu, Yaping Wang, Wenkang Fan, Shicong Hou
Summary: In this study, a feasible design strategy using intramolecular photoinduced electron transfer (PET) process was proposed to obtain a pH-sensitive beta-gal activable fluorescent probe (MLC). The probe selectively reacted with beta-gal and produced strong fluorescence at low pH. The probe demonstrated high selectivity, lower cytotoxicity, lysosomal localization, and fast response time, making it a potentially powerful tool in cancer prevention and treatment.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Cell Biology
Renjie Liao, Manas Mondal, Christopher D. Nazaroff, Diego Mastroeni, Paul D. Coleman, Joshua Labaer, Jia Guo
Summary: The method presented achieves highly sensitive and multiplexed protein analysis using cleavable fluorescent tyramide and off-the-shelf antibodies, enhancing detection sensitivity and reducing imaging time. By studying genetically identical cell populations and profiling neurons in human brain tissue, it reveals protein expression heterogeneity and the presence of distinct neuron clusters within different sub-regions of the hippocampus.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Kaixin Yu, Yufeng Ding, Hua Yu, Wencheng Zhu, Haitao Yu, Yanju Luo, Xujun Zheng, Yan Huang, Zhiyun Lu, Xiongjun Wang
Summary: The observation and discovery of lysosome dynamic alterations using a lysosome-targetable fluorescent probe have led to a better understanding of lysosome biology and the development of new cancer therapeutics. By studying the positioning of lysosomes, a potential anticancer therapy combining TNF alpha and chloroquine has been revealed, with the mechanism potentially involving lysosomal dilation.
Article
Chemistry, Analytical
Xiaofei Liao, Nian Luo, Mengyan Li, Hao Fu, Li Zou
Summary: A novel label-free fluorescent biosensor for highly sensitive detection of bleomycin (BLM) was developed based on BLM-mediated inactivation of CRISPR-Cas12a and thioflavin T (ThT)-induced G-quadruplex formation. The biosensor exhibits high sensitivity and selectivity for detection of BLM in human serum samples, showing promise for BLM assay in biomedical research.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Kentaro Ito, Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku
Summary: A highly sensitive immunoassay for human immunoglobulin G (IgG) using signal amplification of the coagulation cascade is reported. The reaction product, pNA, was quantified using square wave voltammetry with a glassy carbon electrode. Thrombin efficiently reacted with the substrate FPK-pNA and factor XIa was not inactivated during labeling. The coagulation cascade-based immunoassay showed high selectivity and sensitivity for human IgG.
ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Environmental
Shikha Jain, Neeraj Dilbaghi, Nitin Kumar Singhal, Ajeet Kaushik, Ki-Hyun Kim, Sandeep Kumar
Summary: Accumulation of heavy metal ions in the ecosystem is a major concern for their negative effects on animals, humans, and aquatic habitats. A rapid and portable sensing system is crucial for detecting and managing such pollutants. A fluorescence-based biosensor has been developed for the selective and sensitive detection of Pb2+ ions using a DNAzyme system that responds only to the presence of lead (II) ions (Pb2+). This novel biosensing approach offers insights into the rapid and sensitive detection of major pollutants for food and environmental safety.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Electrochemistry
Palraj Kalimuthu, Tobias Kruse, Paul V. Bernhardt
Summary: The electrochemical nitrate biosensor utilizes nitrate reductase from Neurospora crassa, entrapped within a polymeric chitosan matrix on a glassy carbon electrode, with the electron mediator anthraquinone sulfonate. The sensor shows high sensitivity, selectivity, and long-term stability, and is successfully applied for nitrate detection in lake and river water samples.
ELECTROCHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Guo-Xi Liang, Shu-Ying Ye, Hao-Ming Yu, Kai-Ren Zhao, Peng-Fei Liu, Zhi-Jun Liu, Li Wang
Summary: This study proposes a new Zika virus diagnostic platform that combines a DNA walker structure and a localized catalytic hairpin assembly (LCHA) cascade amplification strategy for fast, low-cost, and accurate diagnosis. The method allows for Zika virus sequence detection down to 20 pM without enzymatic DNA amplification, and can detect the virus directly from serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Aneesh Koyappayil, Hyun Tae Kim, Min-Ho Lee
Summary: Fluorescent copper microspheres with high quantum yield were synthesized using an aggressive mechanism, showing bright luminescence under UV light. These microspheres can detect NADH in both low and high concentrations, with a dual-mode sensing platform established for selective detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Tianzi Xia, Guangyan Liu, Junjie Wang, Shili Hou, Shifeng Hou
Summary: The synthesized MXene exhibited biocompatibility, excellent electronic conductivity, and good dispersion in aqueous phase. The Chit/ChOx/Ti3C2Tx nanocomposite was prepared and characterized using SEM, CV, EIS, and DPV. The biosensor for cholesterol detection showed a good linear relationship with low detection limit and high sensitivity, demonstrating practicality in real samples.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Environmental Sciences
Maria I. Gaviria, Kaory Barrientos, Juan Pablo Arango, Juan B. Cano, Gustavo A. Penuela
Summary: In this study, a fluorescent biosensor was developed by conjugating carbon quantum dots with acetylcholinesterase to detect chlorpyrifos in commercial pesticide formulations. The biosensor showed high selectivity and sensitivity, with a limit of detection as low as 0.14 ppb for chlorpyrifos.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2022)