Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM

(2021) Tomasz Uchański et al.   NATURE METHODS

N-terminal transmembrane-helix epitope tag for x-ray crystallography and electron microscopy of small membrane proteins

(2021) Benjamin C. McIlwain et al.   JOURNAL OF MOLECULAR BIOLOGY

Structural insights into the inhibition of glycine reuptake

(2021) Azadeh Shahsavar et al.   NATURE

Structural basis of omega-3 fatty acid transport across the blood–brain barrier

(2021) Rosemary J. Cater et al.   NATURE

Highly accurate protein structure prediction with AlphaFold

(2021) John Jumper et al.   NATURE

Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1

(2021) Qiuyan Chen et al.   NATURE

Structure of an AMPK complex in an inactive, ATP-bound state

(2021) Yan Yan et al.   SCIENCE

Accurate prediction of protein structures and interactions using a three-track neural network

(2021) Minkyung Baek et al.   SCIENCE

Structure and analysis of nanobody binding to the human ASIC1a ion channel

(2021) Yangyu Wu et al.   eLife

Structure and mechanism of the ER-based glucosyltransferase ALG6

(2020) Joël S. Bloch et al.   NATURE

Structure of CD20 in complex with the therapeutic monoclonal antibody rituximab

(2020) Lionel Rougé et al.   SCIENCE

Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins

(2020) Somnath Mukherjee et al.   Nature Communications

Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling

(2020) Naotaka Tsutsumi et al.   eLife

Cryo-electron microscopy analysis of small membrane proteins

(2020) Rie Nygaard et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction

(2020) Ali Punjani et al.   NATURE METHODS

Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor

(2019) Philipp Bräuer et al.   SCIENCE

Nanobody approval gives domain antibodies a boost

(2019) Chris Morrison   NATURE REVIEWS DRUG DISCOVERY

Application of antihelix antibodies in protein structure determination

(2019) Ji Won Kim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural basis of species-selective antagonist binding to the succinate receptor

(2019) Matthias Haffke et al.   NATURE

Structure and drug resistance of the Plasmodium falciparum transporter PfCRT

(2019) Jonathan Kim et al.   NATURE

Structural and thermodynamic basis for the recognition of the substrate-binding cleft on hen egg lysozyme by a single-domain antibody

(2019) Hiroki Akiba et al.   Scientific Reports

Why recombinant antibodies — benefits and applications

(2019) Koli Basu et al.   CURRENT OPINION IN BIOTECHNOLOGY

Development of “Plug and Play” Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM

(2019) Przemyslaw Dutka et al.   STRUCTURE

Structure of the Nanobody-Stabilized Active State of the Kappa Opioid Receptor

(2018) Tao Che et al.   CELL

Locking the Elbow: Improved Antibody Fab Fragments as Chaperones for Structure Determination

(2018) Lucas J. Bailey et al.   JOURNAL OF MOLECULAR BIOLOGY

Structural Basis of Enhanced Crystallizability Induced by a Molecular Chaperone for Antibody Antigen-Binding Fragments

(2018) June Ereño-Orbea et al.   JOURNAL OF MOLECULAR BIOLOGY

Yeast surface display platform for rapid discovery of conformationally selective nanobodies

(2018) Conor McMahon et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Yeast surface display platform for rapid discovery of conformationally selective nanobodies

(2018) Conor McMahon et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Synthetic single domain antibodies for the conformational trapping of membrane proteins

(2018) Iwan Zimmermann et al.   eLife

Cryo-EM structure of human rhodopsin bound to an inhibitory G protein

(2018) Yanyong Kang et al.   NATURE

Collection, pre-processing and on-the-fly analysis of data for high-resolution, single-particle cryo-electron microscopy

(2018) Rebecca F. Thompson et al.   Nature Protocols

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

UCSF ChimeraX: Meeting modern challenges in visualization and analysis

(2017) Thomas D. Goddard et al.   PROTEIN SCIENCE

Clustal Omega for making accurate alignments of many protein sequences

(2017) Fabian Sievers et al.   PROTEIN SCIENCE

Structural basis of nanobody-mediated blocking of BtuF, the cognate substrate-binding protein of the Escherichia coli vitamin B12 transporter BtuCD

(2017) S. A. Mireku et al.   Scientific Reports

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

Structural basis of transcobalamin recognition by human CD320 receptor

(2016) Amer Alam et al.   Nature Communications

ANARCI: antigen receptor numbering and receptor classification

(2015) James Dunbar et al.   BIOINFORMATICS

A High Through-put Platform for Recombinant Antibodies to Folded Proteins

(2015) Michael Hornsby et al.   MOLECULAR & CELLULAR PROTEOMICS

A general protocol for the generation of Nanobodies for structural biology

(2014) Els Pardon et al.   Nature Protocols

Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport

(2014) Ines A Ehrnstorfer et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Nanobody-based products as research and diagnostic tools

(2014) Thomas De Meyer et al.   TRENDS IN BIOTECHNOLOGY

Nanobodies: Natural Single-Domain Antibodies

(2013) Serge Muyldermans   Annual Review of Biochemistry

Synthetic antibody technologies

(2013) Jarrett J Adams et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Activation and allosteric modulation of a muscarinic acetylcholine receptor

(2013) Andrew C. Kruse et al.   NATURE

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

T Cell Receptor-Like Recognition of Tumor In Vivo by Synthetic Antibody Fragment

(2012) Keith R. Miller et al.   PLoS One

Fabs Enable Single Particle cryoEM Studies of Small Proteins

(2012) Shenping Wu et al.   STRUCTURE

Structure of a nanobody-stabilized active state of the β2 adrenoceptor

(2011) Søren G. F. Rasmussen et al.   NATURE

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Structure of a cation-bound multidrug and toxic compound extrusion transporter

(2010) Xiao He et al.   NATURE

A portable RNA sequence whose recognition by a synthetic antibody facilitates structural determination

(2010) Yelena Koldobskaya et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Engineering of recombinant crystallization chaperones

(2009) Shohei Koide   CURRENT OPINION IN STRUCTURAL BIOLOGY