Phage-Assisted Continuous Evolution and Selection of Enzymes for Chemical Synthesis

Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systems

(2021) Timothy C. Yu et al.   Nature Communications

Biosensor-Based Directed Evolution of Methanol Dehydrogenase from Lysinibacillus xylanilyticus

(2021) Thien-Kim Le et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Macrolide Biosensor Optimization through Cellular Substrate Sequestration

(2021) Corwin A. Miller et al.   ACS Synthetic Biology

Systems for in vivo hypermutation: a quest for scale and depth in directed evolution

(2021) Gordon Rix et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

(2020) Michelle F. Richter et al.   NATURE BIOTECHNOLOGY

The developing toolkit of continuous directed evolution

(2020) Mary S. Morrison et al.   Nature Chemical Biology

Automated Continuous Evolution of Proteins in Vivo

(2020) Ziwei Zhong et al.   ACS Synthetic Biology

Phage-assisted continuous and non-continuous evolution

(2020) Shannon M. Miller et al.   Nature Protocols

Design of novel enzyme biocatalysts for industrial bioprocess: Harnessing the power of protein engineering, high throughput screening and synthetic biology

(2020) Aravind Madhavan et al.   BIORESOURCE TECHNOLOGY

Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2

(2019) Timothy B. Roth et al.   ACS Synthetic Biology

Evolution of C‐Terminal Modification Tolerance in Full‐Length and Split T7 RNA Polymerase Biosensors

(2019) Jinyue Pu et al.   CHEMBIOCHEM

The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes

(2019) Ge Qu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells

(2019) Justin G. English et al.   CELL

Development of a Split Esterase for Protein–Protein Interaction-Dependent Small-Molecule Activation

(2019) Krysten A. Jones et al.   ACS Central Science

Site-Selective C–H Halogenation Using Flavin-Dependent Halogenases Identified via Family-Wide Activity Profiling

(2019) Brian F. Fisher et al.   ACS Central Science

A Phage-Assisted Continuous Selection Approach for Deep Mutational Scanning of Protein–Protein Interactions

(2019) Julia Zinkus-Boltz et al.   ACS Chemical Biology

Understanding and Improving the Activity of Flavin-Dependent Halogenases via Random and Targeted Mutagenesis

(2018) Mary C. Andorfer et al.   Annual Review of Biochemistry

Introduction: Biocatalysis in Industry

(2018) Greg Hughes et al.   CHEMICAL REVIEWS

Continuous directed evolution for strain and protein engineering

(2018) Simon d’Oelsnitz et al.   CURRENT OPINION IN BIOTECHNOLOGY

Chemically induced proximity in biology and medicine

(2018) Benjamin Z. Stanton et al.   SCIENCE

Evolution of enzymes with new specificity by high-throughput screening using DmpR-based genetic circuits and multiple flow cytometry rounds

(2018) Kil Koang Kwon et al.   Scientific Reports

An Adaptable Platform for Directed Evolution in Human Cells

(2018) Chet M. Berman et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Evolution of a split RNA polymerase as a versatile biosensor platform

(2017) Jinyue Pu et al.   Nature Chemical Biology

Continuous directed evolution of aminoacyl-tRNA synthetases

(2017) David I Bryson et al.   Nature Chemical Biology

Biocatalysis in the Pharmaceutical Industry: The Need for Speed

(2017) Matthew D. Truppo   ACS Medicinal Chemistry Letters

Phage-assisted continuous evolution of proteases with altered substrate specificity

(2017) Michael S. Packer et al.   Nature Communications

Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering

(2017) Yang Liu et al.   Frontiers in Microbiology

Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance

(2016) Ahmed H. Badran et al.   NATURE

Genetically encoded sensors enable real-time observation of metabolite production

(2016) Jameson K. Rogers et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural Analysis of an Evolved Transketolase Reveals Divergent Binding Modes

(2016) Pierre E. Affaticati et al.   Scientific Reports

Directed Evolution of RebH for Site-Selective Halogenation of Large Biologically Active Molecules

(2015) James T. Payne et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Transcription factor-based biosensors in biotechnology: current state and future prospects

(2015) Regina Mahr et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Esterases as stereoselective biocatalysts

(2015) Diego Romano et al.   BIOTECHNOLOGY ADVANCES

Chemically induced dimerization: reversible and spatiotemporal control of protein function in cells

(2015) Stephanie Voß et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

In vivo continuous directed evolution

(2015) Ahmed H Badran et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Development of biosensors and their application in metabolic engineering

(2015) Jie Zhang et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Applications and advances of metabolite biosensors for metabolic engineering

(2015) Di Liu et al.   METABOLIC ENGINEERING

Continuous directed evolution of DNA-binding proteins to improve TALEN specificity

(2015) Basil P Hubbard et al.   NATURE METHODS

Synthetic biosensors for precise gene control and real-time monitoring of metabolites

(2015) Jameson K. Rogers et al.   NUCLEIC ACIDS RESEARCH

Development of potent in vivo mutagenesis plasmids with broad mutational spectra

(2015) Ahmed H. Badran et al.   Nature Communications

A Competitive Flow Cytometry Screening System for Directed Evolution of Therapeutic Enzyme

(2015) Feng Cheng et al.   ACS Synthetic Biology

Transcription factor-based biosensors enlightened by the analyte

(2015) Raul Fernandez-López et al.   Frontiers in Microbiology

Negative selection and stringency modulation in phage-assisted continuous evolution

(2014) Jacob C Carlson et al.   Nature Chemical Biology

A system for the continuous directed evolution of proteases rapidly reveals drug-resistance mutations

(2014) Bryan C. Dickinson et al.   Nature Communications

Stabilizing biocatalysts

(2013) Andreas S. Bommarius et al.   CHEMICAL SOCIETY REVIEWS

Reporter-based screening and selection of enzymes

(2013) Teunke van Rossum et al.   FEBS Journal

Experimental interrogation of the path dependence and stochasticity of protein evolution using phage-assisted continuous evolution

(2013) B. C. Dickinson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Selective esterase-ester pair for targeting small molecules with cellular specificity

(2012) L. Tian et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

Engineering the ABA Plant Stress Pathway for Regulation of Induced Proximity

(2011) F.-S. Liang et al.   Science Signaling

Combinatorial Alanine Substitution Enables Rapid Optimization of Cytochrome P450BM3 for Selective Hydroxylation of Large Substrates

(2010) Jared C. Lewis et al.   CHEMBIOCHEM

Chemically Controlled Protein Assembly: Techniques and Applications

(2010) Adrian Fegan et al.   CHEMICAL REVIEWS

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

MISER chromatography (multiple injections in a single experimental run): the chromatogram is the graph

(2010) Christopher J. Welch et al.   TETRAHEDRON-ASYMMETRY