Reporter-based screening and selection of enzymes

Computational Enzyme Design

(2013) Gert Kiss et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Isolation and characterization of lignin-degrading bacteria from rainforest soils

(2013) Xing-Feng Huang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Strategies for the discovery and engineering of enzymes for biocatalysis

(2013) Timo Davids et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Flow cytometry-based ultra-high-throughput screening assay for cellulase activity

(2012) Raluca Ostafe et al.   ANALYTICAL BIOCHEMISTRY

Intracellular Detection and Evolution of Site-Specific Proteases Using a Genetic Selection System

(2012) Kathryn D. Verhoeven et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Kinetic and Stoichiometric Characterisation of Streptavidin-Binding Aptamers

(2012) Vincent J. B. Ruigrok et al.   CHEMBIOCHEM

Picoliter Cell Lysate Assays in Microfluidic Droplet Compartments for Directed Enzyme Evolution

(2012) Balint Kintses et al.   CHEMISTRY & BIOLOGY

Ultrahigh Throughput Screening System for Directed Glucose Oxidase Evolution in Yeast Cells

(2012) Radivoje Prodanovic et al.   COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

Bioluminescence based in vivo screening technologies

(2012) Madhura Kelkar et al.   CURRENT OPINION IN PHARMACOLOGY

Directed enzyme evolution: beyond the low-hanging fruit

(2012) Moshe Goldsmith et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

A functional screen for recovery of 4′-phosphopantetheinyl transferase and associated natural product biosynthesis genes from metagenome libraries

(2012) J. G. Owen et al.   ENVIRONMENTAL MICROBIOLOGY

Emerging themes in the computational design of novel enzymes and protein-protein interfaces

(2012) Sagar D. Khare et al.   FEBS LETTERS

Computational Protein Engineering: Bridging the Gap between Rational Design and Laboratory Evolution

(2012) Alexandre Barrozo et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Microplate-based active/inactive 1° screen for biomass degrading enzyme library purification and gene discovery

(2012) Kurt Wagschal et al.   JOURNAL OF MICROBIOLOGICAL METHODS

High-throughput enzyme evolution in Saccharomyces cerevisiae using a synthetic RNA switch

(2012) Joshua K. Michener et al.   METABOLIC ENGINEERING

Binding Pocket Optimization by Computational Protein Design

(2012) Christoph Malisi et al.   PLoS One

A Computational Methodology to Screen Activities of Enzyme Variants

(2012) Martin R. Hediger et al.   PLoS One

Comparison of designed and randomly generated catalysts for simple chemical reactions

(2012) Yakov Kipnis et al.   PROTEIN SCIENCE

Development of a high-throughput screening method for racemase activity and its application to the identification of alanine racemase variants with activity towards l-arginine

(2012) Simon C. Willies et al.   TETRAHEDRON

Nature Versus Nurture: Developing Enzymes That Function Under Extreme Conditions

(2012) Michael J. Liszka et al.   Annual Review of Chemical and Biomolecular Engineering

DIRECTED EVOLUTION: SELECTION OF THE HOST ORGANISM

(2012) Azadeh Pourmir et al.   Computational and Structural Biotechnology Journal

A Combination of In Vivo Selection and Cell Sorting for the Identification of Enantioselective Biocatalysts

(2011) Elena Fernández-Álvaro et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Alternative affinity tools: more attractive than antibodies?

(2011) Vincent J. B. Ruigrok et al.   BIOCHEMICAL JOURNAL

Recent trends in biocatalysis engineering

(2011) Andrés Illanes et al.   BIORESOURCE TECHNOLOGY

Protein and RNA engineering to customize microbial molecular reporting

(2011) Joseph A. Gredell et al.   Biotechnology Journal

Advances in directed molecular evolution of reporter genes

(2011) Ai-Sheng Xiong et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Functional metagenomic strategies for the discovery of novel enzymes and biosurfactants with biotechnological applications from marine ecosystems

(2011) J. Kennedy et al.   JOURNAL OF APPLIED MICROBIOLOGY

Applications of genetically-encoded biosensors for the construction and control of biosynthetic pathways

(2011) Joshua K. Michener et al.   METABOLIC ENGINEERING

Association of dnt genes of Burkholderia sp. DNT with the substrate-blind regulator DntR draws the evolutionary itinerary of 2,4-dinitrotoluene biodegradation

(2011) Aitor de las Heras et al.   MOLECULAR MICROBIOLOGY

Substrate Profiling of Tobacco Etch Virus Protease Using a Novel Fluorescence-Assisted Whole-Cell Assay

(2011) George Kostallas et al.   PLoS One

A genetically encoded, high-signal-to-noise maltose sensor

(2011) Jonathan S. Marvin et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Strategies for discovery and improvement of enzyme function: state of the art and opportunities

(2011) Praveen Kaul et al.   Microbial Biotechnology

Design and Application of a Mevalonate-Responsive Regulatory Protein

(2010) Shuang-Yan Tang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

High-Throughput Metabolic Engineering: Advances in Small-Molecule Screening and Selection

(2010) Jeffrey A. Dietrich et al.   Annual Review of Biochemistry

Product-Induced Gene Expression, a Product-Responsive Reporter Assay Used To Screen Metagenomic Libraries for Enzyme-Encoding Genes

(2010) T. Uchiyama et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Metagenomic era for biocatalyst identification

(2010) Lucía Fernández-Arrojo et al.   CURRENT OPINION IN BIOTECHNOLOGY

Fluorescence Activated Cell Sorting as a General Ultra-High-Throughput Screening Method for Directed Evolution of Glycosyltransferases

(2010) Guangyu Yang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Reprogramming bacteria to seek and destroy an herbicide

(2010) Joy Sinha et al.   Nature Chemical Biology

Enzymes and Bioconversions of Industrial, Pharmaceutical, and Biotechnological Significance

(2010) Sergio Sanchez et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Ultrahigh-Throughput FACS-Based Screening for Directed Enzyme Evolution

(2009) Guangyu Yang et al.   CHEMBIOCHEM

Functional metagenomics for enzyme discovery: challenges to efficient screening

(2009) Taku Uchiyama et al.   CURRENT OPINION IN BIOTECHNOLOGY

Directed evolution of enzymes: Library screening strategies

(2009) Hans Leemhuis et al.   IUBMB LIFE

Computational design of ligand binding is not a solved problem

(2009) B. Schreier et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-Throughput Selection for Cellulase Catalysts Using Chemical Complementation

(2008) Pamela Peralta-Yahya et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed enzyme evolution via small and effective neutral drift libraries

(2008) Rinkoo D Gupta et al.   NATURE METHODS

Substrate-induced gene expression (SIGEX) screening of metagenome libraries

(2008) Taku Uchiyama et al.   Nature Protocols

Tracing explosives in soil with transcriptional regulators of Pseudomonas putida evolved for responding to nitrotoluenes

(2008) Junkal Garmendia et al.   Microbial Biotechnology