The developing toolkit of continuous directed evolution

Continuous evolution of SpCas9 variants compatible with non-G PAMs

(2020) Shannon M. Miller et al.   NATURE BIOTECHNOLOGY

Machine learning-assisted directed protein evolution with combinatorial libraries

(2019) Zachary Wu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells

(2019) Justin G. English et al.   CELL

Continuous evolution of base editors with expanded target compatibility and improved activity

(2019) Benjamin W. Thuronyi et al.   NATURE BIOTECHNOLOGY

A Processive Protein Chimera Introduces Mutations across Defined DNA Regions In Vivo

(2018) Christopher L. Moore et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Precise, automated control of conditions for high-throughput growth of yeast and bacteria with eVOLVER

(2018) Brandon G Wong et al.   NATURE BIOTECHNOLOGY

A designer enzyme for hydrazone and oxime formation featuring an unnatural catalytic aniline residue

(2018) Ivana Drienovská et al.   Nature Chemistry

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window

(2018) Shakked O. Halperin et al.   NATURE

Continuous directed evolution of proteins with improved soluble expression

(2018) Tina Wang et al.   Nature Chemical Biology

Retroelement-based genome editing and evolution

(2018) Anna J. Simon et al.   ACS Synthetic Biology

Directed Evolution of a Designer Enzyme Featuring an Unnatural Catalytic Amino Acid

(2018) Clemens Mayer et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Scalable, Continuous Evolution of Genes at Mutation Rates above Genomic Error Thresholds

(2018) Arjun Ravikumar et al.   CELL

An Adaptable Platform for Directed Evolution in Human Cells

(2018) Chet M. Berman et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Increased production of L-serine in Escherichia coli through Adaptive Laboratory Evolution

(2017) Hemanshu Mundhada et al.   METABOLIC ENGINEERING

Evolution of a designed protein assembly encapsulating its own RNA genome

(2017) Gabriel L. Butterfield et al.   NATURE

Continuous directed evolution of aminoacyl-tRNA synthetases

(2017) David I Bryson et al.   Nature Chemical Biology

Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity

(2017) Stanislav S. Terekhov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Simulation of the M13 life cycle I: Assembly of a genetically-structured deterministic chemical kinetic simulation

(2017) Steven W. Smeal et al.   VIROLOGY

A multiplex culture system for the long-term growth of fission yeast cells

(2017) Céline Callens et al.   YEAST

Phage-assisted continuous evolution of proteases with altered substrate specificity

(2017) Michael S. Packer et al.   Nature Communications

Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance

(2016) Ahmed H. Badran et al.   NATURE

Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering

(2016) Andrew D Garst et al.   NATURE BIOTECHNOLOGY

Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells

(2016) Gaelen T Hess et al.   NATURE METHODS

In vivo continuous evolution of genes and pathways in yeast

(2016) Nathan Crook et al.   Nature Communications

Rapid and Inexpensive Evaluation of Nonstandard Amino Acid Incorporation in Escherichia coli

(2016) Jordan W. Monk et al.   ACS Synthetic Biology

Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids

(2015) Miriam Amiram et al.   NATURE BIOTECHNOLOGY

Continuous directed evolution of DNA-binding proteins to improve TALEN specificity

(2015) Basil P Hubbard et al.   NATURE METHODS

Development of potent in vivo mutagenesis plasmids with broad mutational spectra

(2015) Ahmed H. Badran et al.   Nature Communications

Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness

(2014) Michael J Hammerling et al.   Nature Chemical Biology

Negative selection and stringency modulation in phage-assisted continuous evolution

(2014) Jacob C Carlson et al.   Nature Chemical Biology

An orthogonal DNA replication system in yeast

(2014) Arjun Ravikumar et al.   Nature Chemical Biology

A system for the continuous directed evolution of proteases rapidly reveals drug-resistance mutations

(2014) Bryan C. Dickinson et al.   Nature Communications

A Low Cost, Customizable Turbidostat for Use in Synthetic Circuit Characterization

(2014) Chris N. Takahashi et al.   ACS Synthetic Biology

Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM

(2013) Shawn P. Finney-Manchester et al.   NUCLEIC ACIDS RESEARCH

A Heritable Recombination System for Synthetic Darwinian Evolution in Yeast

(2012) Dante W. Romanini et al.   ACS Synthetic Biology

Chemical Evolution of a Bacterium’s Genome

(2011) Philippe Marlière et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

Evolutionary paths to antibiotic resistance under dynamically sustained drug selection

(2011) Erdal Toprak et al.   NATURE GENETICS

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE