Systems for in vivo hypermutation: a quest for scale and depth in directed evolution

Engineering new catalytic activities in enzymes

(2020) Kai Chen et al.   Nature Catalysis

Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

(2020) Michelle F. Richter et al.   NATURE BIOTECHNOLOGY

The developing toolkit of continuous directed evolution

(2020) Mary S. Morrison et al.   Nature Chemical Biology

Targeted Diversification in the S. cerevisiae Genome with CRISPR-Guided DNA Polymerase I

(2020) Connor J. Tou et al.   ACS Synthetic Biology

Accelerated evolution of a minimal 63–amino acid dual transcription factor

(2020) Andreas K. Brödel et al.   Science Advances

Phage-assisted continuous and non-continuous evolution

(2020) Shannon M. Miller et al.   Nature Protocols

In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9

(2020) Beatriz Álvarez et al.   Nature Communications

Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2

(2019) Timothy B. Roth et al.   ACS Synthetic Biology

VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells

(2019) Justin G. English et al.   CELL

Synthetic evolution

(2019) Anna J. Simon et al.   NATURE BIOTECHNOLOGY

Machine-learning-guided directed evolution for protein engineering

(2019) Kevin K. Yang et al.   NATURE METHODS

A Phage-Assisted Continuous Selection Approach for Deep Mutational Scanning of Protein–Protein Interactions

(2019) Julia Zinkus-Boltz et al.   ACS Chemical Biology

Protein Structure from Experimental Evolution

(2019) Michael A. Stiffler et al.   Cell Systems

A Processive Protein Chimera Introduces Mutations across Defined DNA Regions In Vivo

(2018) Christopher L. Moore et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window

(2018) Shakked O. Halperin et al.   NATURE

Continuous directed evolution of proteins with improved soluble expression

(2018) Tina Wang et al.   Nature Chemical Biology

Scalable, Continuous Evolution of Genes at Mutation Rates above Genomic Error Thresholds

(2018) Arjun Ravikumar et al.   CELL

An Adaptable Platform for Directed Evolution in Human Cells

(2018) Chet M. Berman et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Tunable Expression Systems for Orthogonal DNA Replication

(2018) Ziwei Zhong et al.   ACS Synthetic Biology

Evolution of a split RNA polymerase as a versatile biosensor platform

(2017) Jinyue Pu et al.   Nature Chemical Biology

Continuous directed evolution of aminoacyl-tRNA synthetases

(2017) David I Bryson et al.   Nature Chemical Biology

Phage-assisted continuous evolution of proteases with altered substrate specificity

(2017) Michael S. Packer et al.   Nature Communications

Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance

(2016) Ahmed H. Badran et al.   NATURE

Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells

(2016) Gaelen T Hess et al.   NATURE METHODS

In vivo continuous evolution of genes and pathways in yeast

(2016) Nathan Crook et al.   Nature Communications

An orthogonal DNA replication system in yeast

(2014) Arjun Ravikumar et al.   Nature Chemical Biology

Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM

(2013) Shawn P. Finney-Manchester et al.   NUCLEIC ACIDS RESEARCH

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

Correction: Mutator Suppression and Escape from Replication Error–Induced Extinction in Yeast

(2011) Alan J. Herr et al.   PLoS Genetics