Evolution of enzymes with new specificity by high-throughput screening using DmpR-based genetic circuits and multiple flow cytometry rounds

Protein engineering approaches to chemical biotechnology

(2016) Zhen Chen et al.   CURRENT OPINION IN BIOTECHNOLOGY

A novel psychrophilic alkaline phosphatase from the metagenome of tidal flat sediments

(2015) Dae-Hee Lee et al.   BMC BIOTECHNOLOGY

A Fluorescent Hydrogel-Based Flow Cytometry High-Throughput Screening Platform for Hydrolytic Enzymes

(2014) Christian Pitzler et al.   CHEMISTRY & BIOLOGY

Engineering anAcinetobacterregulon for biosensing and high-throughput enzyme screening inE. colivia flow cytometry

(2014) Ramesh K. Jha et al.   NUCLEIC ACIDS RESEARCH

Toward a Generalized and High-throughput Enzyme Screening System Based on Artificial Genetic Circuits

(2013) Su-Lim Choi et al.   ACS Synthetic Biology

Exploring the Evolution of Novel Enzyme Functions within Structurally Defined Protein Superfamilies

(2012) Nicholas Furnham et al.   PLoS Computational Biology

Single mutations outside the active site affect the substrate specificity in a β-glycosidase

(2011) Lúcio M.F. Mendonça et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Protein design in metabolic engineering and synthetic biology

(2011) Jürgen Pleiss   CURRENT OPINION IN BIOTECHNOLOGY

Toward a Systems Biology Perspective on Enzyme Evolution

(2011) Shelley D. Copley   JOURNAL OF BIOLOGICAL CHEMISTRY

Crystallographic Snapshots of Tyrosine Phenol-lyase Show That Substrate Strain Plays a Role in C–C Bond Cleavage

(2011) Dalibor Milić et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enzyme Promiscuity: A Mechanistic and Evolutionary Perspective

(2010) Olga Khersonsky and Dan S. Tawfik   Annual Review of Biochemistry

Properties of tryptophan indole-lyase from a piezophilic bacterium, Photobacterium profundum SS9

(2010) Robert S. Phillips et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Beyond directed evolution—semi-rational protein engineering and design

(2010) Stefan Lutz   CURRENT OPINION IN BIOTECHNOLOGY

Design strategies for the creation of artificial metalloenzymes

(2010) Tillmann Heinisch et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Enzyme (re)design: lessons from natural evolution and computation

(2009) John A Gerlt et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

CHARMM: The biomolecular simulation program

(2009) B. R. Brooks et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Insights into the Catalytic Mechanism of Tyrosine Phenol-lyase from X-ray Structures of Quinonoid Intermediates

(2008) Dalibor Milić et al.   JOURNAL OF BIOLOGICAL CHEMISTRY