Evolution of a split RNA polymerase as a versatile biosensor platform

Chemical and Biophysical Modulation of Cas9 for Tunable Genome Engineering

(2016) James K. Nuñez et al.   ACS Chemical Biology

Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance

(2016) Ahmed H. Badran et al.   NATURE

A transcription activator–like effector (TALE) induction system mediated by proteolysis

(2016) Matthew F Copeland et al.   Nature Chemical Biology

In-Gel Imaging of RNA Processing Using Broccoli Reveals Optimal Aptamer Expression Strategies

(2015) Grigory S. Filonov et al.   CHEMISTRY & BIOLOGY

Development of biosensors and their application in metabolic engineering

(2015) Jie Zhang et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

A Panel of Protease-Responsive RNA Polymerases Respond to Biochemical Signals by Production of Defined RNA Outputs in Live Cells

(2015) Jinyue Pu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

(2015) Lauren R Polstein et al.   Nature Chemical Biology

Continuous directed evolution of DNA-binding proteins to improve TALEN specificity

(2015) Basil P Hubbard et al.   NATURE METHODS

Methods for the directed evolution of proteins

(2015) Michael S. Packer et al.   NATURE REVIEWS GENETICS

Toehold Switches: De-Novo-Designed Regulators of Gene Expression

(2014) Alexander A. Green et al.   CELL

A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase

(2014) T. H. Segall-Shapiro et al.   Molecular Systems Biology

Negative selection and stringency modulation in phage-assisted continuous evolution

(2014) Jacob C Carlson et al.   Nature Chemical Biology

Principles of genetic circuit design

(2014) Jennifer A N Brophy et al.   NATURE METHODS

Synthetic biology in mammalian cells: next generation research tools and therapeutics

(2014) Florian Lienert et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Realizing the potential of synthetic biology

(2014) George M. Church et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins

(2014) Gurkan Guntas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Highly Multiplexed Subcellular RNA Sequencing in Situ

(2014) J. H. Lee et al.   SCIENCE

A system for the continuous directed evolution of proteases rapidly reveals drug-resistance mutations

(2014) Bryan C. Dickinson et al.   Nature Communications

A Population-Based Experimental Model for Protein Evolution: Effects of Mutation Rate and Selection Stringency on Evolutionary Outcomes

(2013) Aaron M. Leconte et al.   BIOCHEMISTRY

Optical control of mammalian endogenous transcription and epigenetic states

(2013) Silvana Konermann et al.   NATURE

Directed evolution of genetic parts and circuits by compartmentalized partnered replication

(2013) Jared W Ellefson et al.   NATURE BIOTECHNOLOGY

Experimental interrogation of the path dependence and stochasticity of protein evolution using phage-assisted continuous evolution

(2013) B. C. Dickinson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants

(2013) D. L. Shis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fifteen years of the yeast three-hybrid system: RNA–protein interactions under investigation

(2012) Franck Martin   METHODS

Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting protein–protein interactions in vivo

(2012) Brett D. Blakeley et al.   Molecular BioSystems

Measuring Cation Dependent DNA Polymerase Fidelity Landscapes by Deep Sequencing

(2012) Bradley Michael Zamft et al.   PLoS One

Split-protein systems: beyond binary protein–protein interactions

(2011) Sujan S Shekhawat et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

Synthetic Biology Moving into the Clinic

(2011) W. C. Ruder et al.   SCIENCE

Reprogramming Cellular Behavior with RNA Controllers Responsive to Endogenous Proteins

(2010) S. J. Culler et al.   SCIENCE

Visualization of molecular interactions using bimolecular fluorescence complementation analysis: Characteristics of protein fragment complementation

(2009) Tom K. Kerppola   CHEMICAL SOCIETY REVIEWS

RNA-based computation in live cells

(2009) Yaakov Benenson   CURRENT OPINION IN BIOTECHNOLOGY

The structural changes of T7 RNA polymerase from transcription initiation to elongation

(2009) Thomas A Steitz   CURRENT OPINION IN STRUCTURAL BIOLOGY

A Novel Estrogen Receptor Intramolecular Folding–based Titratable Transgene Expression System

(2009) Ramasamy Paulmurugan et al.   MOLECULAR THERAPY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Bimolecular Fluorescence Complementation (BiFC) Analysis as a Probe of Protein Interactions in Living Cells

(2008) Tom K. Kerppola   Annual Review of Biophysics