Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins

Thermostabilization of membrane proteins by consensus mutation: a case study for a fungal Δ8-7 sterol isomerase

(2020) Hebang Yao et al.   JOURNAL OF MOLECULAR BIOLOGY

A novel coronavirus outbreak of global health concern

(2020) Chen Wang et al.   LANCET

A new coronavirus associated with human respiratory disease in China

(2020) Fan Wu et al.   NATURE

A Novel Coronavirus from Patients with Pneumonia in China, 2019

(2020) Na Zhu et al.   NEW ENGLAND JOURNAL OF MEDICINE

High-throughput stability screening for detergent-solubilized membrane proteins

(2019) Vadim Kotov et al.   Scientific Reports

Molecular mechanisms of human P2X3 receptor channel activation and modulation by divalent cation bound ATP

(2019) Mufeng Li et al.   eLife

The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications

(2019) Hansjörg Götzke et al.   Nature Communications

Druggable negative allosteric site of P2X3 receptors

(2018) Jin Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cryo-EM structure of 5-HT3A receptor in its resting conformation

(2018) Sandip Basak et al.   Nature Communications

Structural principles of distinct assemblies of the human α4β2 nicotinic receptor

(2018) Richard M. Walsh et al.   NATURE

Cryo-EM structure of the benzodiazepine-sensitive α1β1γ2S tri-heteromeric GABAA receptor in complex with GABA

(2018) Swastik Phulera et al.   eLife

An engineered thermal-shift screen reveals specific lipid preferences of eukaryotic and prokaryotic membrane proteins

(2018) Emmanuel Nji et al.   Nature Communications

New tools for automated high-resolution cryo-EM structure determination in RELION-3

(2018) Jasenko Zivanov et al.   eLife

Cryo-EM structures of the triheteromeric NMDA receptor and its allosteric modulation

(2017) Wei Lü et al.   SCIENCE

Crystal structures of a ZIP zinc transporter reveal a binuclear metal center in the transport pathway

(2017) Tuo Zhang et al.   Science Advances

Copper and protons directly activate the zinc-activated channel

(2016) Sarah M. Trattnig et al.   BIOCHEMICAL PHARMACOLOGY

Current strategies for protein production and purification enabling membrane protein structural biology

(2016) Aditya Pandey et al.   Biochemistry and Cell Biology

X-ray structures define human P2X3 receptor gating cycle and antagonist action

(2016) Steven E. Mansoor et al.   NATURE

X-ray structures and mechanism of the human serotonin transporter

(2016) Jonathan A. Coleman et al.   NATURE

Manipulation of Subunit Stoichiometry in Heteromeric Membrane Proteins

(2016) Claudio L. Morales-Perez et al.   STRUCTURE

Peptides in headlock – a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopy

(2016) Michael B. Braun et al.   Scientific Reports

Cryo-EM: A Unique Tool for the Visualization of Macromolecular Complexity

(2015) Eva Nogales et al.   MOLECULAR CELL

Crystal structure of human glycine receptor-α3 bound to antagonist strychnine

(2015) Xin Huang et al.   NATURE

Glycine receptor mechanism elucidated by electron cryo-microscopy

(2015) Juan Du et al.   NATURE

Crystal structure of a human GABAA receptor

(2014) Paul S. Miller et al.   NATURE

NMDA receptor structures reveal subunit arrangement and pore architecture

(2014) Chia-Hsueh Lee et al.   NATURE

Screening and large-scale expression of membrane proteins in mammalian cells for structural studies

(2014) April Goehring et al.   Nature Protocols

Nanobodies: Natural Single-Domain Antibodies

(2013) Serge Muyldermans   Annual Review of Biochemistry

X-ray structure of dopamine transporter elucidates antidepressant mechanism

(2013) Aravind Penmatsa et al.   NATURE

Multicolour Fluorescence-Detection Size-Exclusion Chromatography for Structural Genomics of Membrane Multiprotein Complexes

(2013) David Parcej et al.   PLoS One

Fluorescent probe for high-throughput screening of membrane protein expression

(2013) A. E. Backmark et al.   PROTEIN SCIENCE

Nonionic Homopolymeric Amphipols: Application to Membrane Protein Folding, Cell-Free Synthesis, and Solution Nuclear Magnetic Resonance

(2012) Paola Bazzacco et al.   BIOCHEMISTRY

Non-Ionic Amphiphilic Homopolymers: Synthesis, Solution Properties, and Biochemical Validation

(2012) K. Shivaji Sharma et al.   LANGMUIR

A Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Protein Precrystallization Screening

(2012) Motoyuki Hattori et al.   STRUCTURE

Simple screening method for improving membrane protein thermostability

(2011) Romina Mancusso et al.   METHODS

Principles of activation and permeation in an anion-selective Cys-loop receptor

(2011) Ryan E. Hibbs et al.   NATURE

Structure of a nanobody-stabilized active state of the β2 adrenoceptor

(2011) Søren G. F. Rasmussen et al.   NATURE

Structure and function of an irreversible agonist-β2 adrenoceptor complex

(2011) Daniel M. Rosenbaum et al.   NATURE

Overcoming barriers to membrane protein structure determination

(2011) Roslyn M Bill et al.   NATURE BIOTECHNOLOGY

Bridging the gap: A GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini

(2010) Jennifer M. Hsieh et al.   PROTEIN SCIENCE

Design of stable α-helices using global sequence optimization

(2009) Michael Petukhov et al.   JOURNAL OF PEPTIDE SCIENCE

X-ray structure, symmetry and mechanism of an AMPA-subtype glutamate receptor

(2009) Alexander I. Sobolevsky et al.   NATURE

Crystal structure of the ATP-gated P2X4 ion channel in the closed state

(2009) Toshimitsu Kawate et al.   NATURE

Structure and Mechanism of a Na+-Independent Amino Acid Transporter

(2009) P. L. Shaffer et al.   SCIENCE

GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

(2008) David Drew et al.   Nature Protocols

Microscale Fluorescent Thermal Stability Assay for Membrane Proteins

(2008) Alexander I. Alexandrov et al.   STRUCTURE