CRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement

Target binding and residence: a new determinant of DNA double-strand break repair pathway choice in CRISPR/Cas9 genome editing

(2021) Yili Feng et al.   Journal of Zhejiang University-SCIENCE B

DNA repair protein RAD51 enhances the CRISPR/Cas9-mediated knock-in efficiency in brain neurons

(2020) Taiga Kurihara et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Heterotrimeric G protein are involved in the regulation of multiple agronomic traits and stress tolerance in rice

(2020) Yue Cui et al.   BMC PLANT BIOLOGY

The promise and challenge of therapeutic genome editing

(2020) Jennifer A. Doudna   NATURE

Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors

(2020) Chao Li et al.   NATURE BIOTECHNOLOGY

Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors

(2020) Jordan L. Doman et al.   NATURE BIOTECHNOLOGY

Continuous evolution of SpCas9 variants compatible with non-G PAMs

(2020) Shannon M. Miller et al.   NATURE BIOTECHNOLOGY

Pectin homogalacturonan nanofilament expansion drives morphogenesis in plant epidermal cells

(2020) Kalina T. Haas et al.   SCIENCE

CRISPR-engineered T cells in patients with refractory cancer

(2020) Edward A. Stadtmauer et al.   SCIENCE

Expanding the genome-targeting scope and the site selectivity of high-precision base editors

(2020) Junjie Tan et al.   Nature Communications

Generation of mouse model of TGFBI-R124C corneal dystrophy using CRISPR/Cas9-mediated homology-directed repair

(2020) Kohdai Kitamoto et al.   Scientific Reports

Reduced Enzymatic Browning in Potato Tubers by Specific Editing of a Polyphenol Oxidase Gene via Ribonucleoprotein Complexes Delivery of the CRISPR/Cas9 System

(2020) Matías Nicolás González et al.   Frontiers in Plant Science

Down Regulation and Loss of Auxin Response Factor 4 Function Using CRISPR/Cas9 Alters Plant Growth, Stomatal Function and Improves Tomato Tolerance to Salinity and Osmotic Stress

(2020) Sarah Bouzroud et al.   Genes

Design of high-oleic tobacco (Nicotiana tabacum L.) seed oil by CRISPR-Cas9-mediated knockout of NtFAD2–2

(2020) Yinshuai Tian et al.   BMC PLANT BIOLOGY

Targeted Knockout of BnTT2 Homologues for Yellow-Seeded Brassica napus with Reduced Flavonoids and Improved Fatty Acid Composition

(2020) Tao Xie et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Developing gluten-free cereals and the role of proteomics in product safety

(2020) Angéla Juhász et al.   JOURNAL OF CEREAL SCIENCE

An overview of COVID-19

(2020) Yu Shi et al.   Journal of Zhejiang University-SCIENCE B

CRISPR/Cas9 directed editing of lycopene epsilon-cyclase modulates metabolic flux for β-carotene biosynthesis in banana fruit

(2020) Navneet Kaur et al.   METABOLIC ENGINEERING

Plant Prime Editors Enable Precise Gene Editing in Rice Cells

(2020) Xu Tang et al.   Molecular Plant

Precise Modifications of Both Exogenous and Endogenous Genes in Rice by Prime Editing

(2020) Huiyuan Li et al.   Molecular Plant

Versatile Nucleotides Substitution in Plant Using an Improved Prime Editing System

(2020) Wen Xu et al.   Molecular Plant

Directed evolution of adenine base editors with increased activity and therapeutic application

(2020) Nicole M. Gaudelli et al.   NATURE BIOTECHNOLOGY

Prime genome editing in rice and wheat

(2020) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

(2020) Michelle F. Richter et al.   NATURE BIOTECHNOLOGY

CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

Safety and feasibility of CRISPR-edited T cells in patients with refractory non-small-cell lung cancer

(2020) You Lu et al.   NATURE MEDICINE

Elevating seed oil content in a polyploid crop by induced mutations in SEED FATTY ACID REDUCER genes

(2020) Nirosha L. Karunarathna et al.   PLANT BIOTECHNOLOGY JOURNAL

Gene editing of three BnITPK genes in tetraploid oilseed rape leads to significant reduction of phytic acid in seeds

(2020) Niharika Sashidhar et al.   PLANT BIOTECHNOLOGY JOURNAL

Loss of function of CRT1a (calreticulin) reduces plant susceptibility to Verticillium longisporum in both Arabidopsis thaliana and oilseed rape ( Brassica napus )

(2020) Michael Pröbsting et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas9-mediated mutagenesis of Clpsk1 in watermelon to confer resistance to Fusarium oxysporum f.sp. niveum

(2020) Man Zhang et al.   PLANT CELL REPORTS

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

(2020) Russell T. Walton et al.   SCIENCE

Modifications of fatty acid profile through targeted mutation at BnaFAD2 gene with CRISPR/Cas9-mediated gene editing in Brassica napus

(2020) Huibin Huang et al.   THEORETICAL AND APPLIED GENETICS

SpRY: Engineered CRISPR/Cas9 Harnesses New Genome-Editing Power

(2020) Dangquan Zhang et al.   TRENDS IN GENETICS

Targeted DNA demethylation of the Fgf21 promoter by CRISPR/dCas9-mediated epigenome editing

(2020) Nozomi Hanzawa et al.   Scientific Reports

In-Frame and Frame-Shift Editing of the Ehd1 Gene to Develop Japonica Rice With Prolonged Basic Vegetative Growth Periods

(2020) Mingji Wu et al.   Frontiers in Plant Science

Rapid and sensitive detection of COVID-19 using CRISPR/Cas12a-based detection with naked eye readout, CRISPR/Cas12a-NER

(2020) Xinjie Wang et al.   Science Bulletin

CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew

(2020) Miguel I. Santillán Martínez et al.   BMC PLANT BIOLOGY

Soybean AP1 homologs control flowering time and plant height

(2020) Liyu Chen et al.   Journal of Integrative Plant Biology

Gene editing: an instrument for practical application of gene biology to plant breeding

(2020) Yuan-yuan Tan et al.   Journal of Zhejiang University-SCIENCE B

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

(2020) Ibrahim C. Kurt et al.   NATURE BIOTECHNOLOGY

Glycosylase base editors enable C-to-A and C-to-G base changes

(2020) Dongdong Zhao et al.   NATURE BIOTECHNOLOGY

In vivo locus-specific editing of the neuroepigenome

(2020) Yun Young Yim et al.   NATURE REVIEWS NEUROSCIENCE

Pectin Drives Cell Wall Morphogenesis without Turgor Pressure

(2020) Dangquan Zhang et al.   TRENDS IN PLANT SCIENCE

High-fidelity SaCas9 identified by directional screening in human cells

(2020) Haihua Xie et al.   PLOS BIOLOGY

Knockout of butyrophilin subfamily 1 member A1 (BTN1A1) alters lipid droplet formation and phospholipid composition in bovine mammary epithelial cells

(2020) Liqiang Han et al.   Journal of Animal Science and Biotechnology

CRISPR/Cas9-mediated mutagenesis of VvMLO3 results in enhanced resistance to powdery mildew in grapevine (Vitis vinifera)

(2020) Dong-Yan Wan et al.   Horticulture Research

Gene Therapy in Rare Respiratory Diseases: What Have We Learned So Far?

(2020) Lucía Bañuls et al.   Journal of Clinical Medicine

GmNMHC5, A Neoteric Positive Transcription Factor of Flowering and Maturity in Soybean

(2020) Wenting Wang et al.   Plants-Basel

Efficient targeted mutagenesis in allotetraploid sweet basil by CRISPR/Cas9

(2020) Natasha Navet et al.   Plant Direct

Inhibition of DNA ligase IV enhances the CRISPR/Cas9-mediated knock-in efficiency in mouse brain neurons

(2020) Xueshan Cao et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

CRISPR/cas systems redefine nucleic acid detection: Principles and methods

(2020) Meng Wang et al.   BIOSENSORS & BIOELECTRONICS

CRISPR/Cas gene therapy

(2020) Baohong Zhang   JOURNAL OF CELLULAR PHYSIOLOGY

Rapid, sensitive and specific SARS coronavirus-2 detection: a multi-center comparison between standard qRT-PCR and CRISPR based DETECTR

(2020) Eelke Brandsma et al.   JOURNAL OF INFECTIOUS DISEASES

CRISPR-based assays for rapid detection of SARS-CoV-2

(2020) Vivek S. Javalkote et al.   METHODS

Functional classification of prostate cancer‑associated miRNAs through CRISPR/Cas9‑mediated gene knockout

(2020) Fu‑Neng Jiang et al.   Molecular Medicine Reports

From Sequencing to Genome Editing for Cotton Improvement

(2020) Renhai Peng et al.   TRENDS IN BIOTECHNOLOGY

iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2

(2020) Zahir Ali et al.   VIRUS RESEARCH

Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay

(2020) Xiong Ding et al.   Nature Communications

Controlled Cycling and Quiescence Enables Efficient HDR in Engraftment-Enriched Adult Hematopoietic Stem and Progenitor Cells

(2020) Jiyung J. Shin et al.   Cell Reports

Edible Plant Oil: Global Status, Health Issues, and Perspectives

(2020) Ying Zhou et al.   Frontiers in Plant Science

Functional Analysis of the teosinte branched 1 Gene in the Tetraploid Switchgrass (Panicum virgatum L.) by CRISPR/Cas9-Directed Mutagenesis

(2020) Yang Liu et al.   Frontiers in Plant Science

Engineering Smut Resistance in Maize by Site-Directed Mutagenesis of LIPOXYGENASE 3

(2020) Krishna Mohan Pathi et al.   Frontiers in Plant Science

Color-related chlorophyll and carotenoid concentrations of Chinese kale can be altered through CRISPR/Cas9 targeted editing of the carotenoid isomerase gene BoaCRTISO

(2020) Bo Sun et al.   Horticulture Research

CRISPR/Cas: a powerful tool for gene function study and crop improvement

(2020) Dangquan Zhang et al.   Journal of Advanced Research

Rapid and accurate detection of African swine fever virus by DNA endonuclease-targeted CRISPR trans reporter assay

(2020) Zongjie Li et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

CRISPR screen identifies genes that sensitize AML cells to double negative T cell therapy

(2020) Fraser Soares et al.   BLOOD

Targeted mutagenesis of ∆5 and ∆6 fatty acyl desaturases induce dysregulation of lipid metabolism in Atlantic salmon (Salmo salar)

(2020) Yang Jin et al.   BMC GENOMICS

AKT1 E17K Inhibits Cancer Cell Migration by Abrogating β-Catenin Signaling

(2020) Sizhi Paul Gao et al.   MOLECULAR CANCER RESEARCH

3D Culture Models with CRISPR Screens Reveal Hyperactive NRF2 as a Prerequisite for Spheroid Formation via Regulation of Proliferation and Ferroptosis

(2020) Nobuaki Takahashi et al.   MOLECULAR CELL

Gene therapy using haematopoietic stem and progenitor cells

(2020) Giuliana Ferrari et al.   NATURE REVIEWS GENETICS

High‐oleic acid content, nontransgenic allotetraploid cotton ( Gossypium hirsutum L.) generated by knockout of GhFAD2 genes with CRISPR/Cas9 system

(2020) Yizhen Chen et al.   PLANT BIOTECHNOLOGY JOURNAL

A CRISPR/Cas9‐based genome‐editing system for yam ( Dioscorea spp.)

(2020) Easter D. Syombua et al.   PLANT BIOTECHNOLOGY JOURNAL

Modification of starch composition, structure and properties through editing of TaSBEIIa in both winter and spring wheat varieties by CRISPR/Cas9

(2020) Jingying Li et al.   PLANT BIOTECHNOLOGY JOURNAL

Next-Generation CRISPR Technologies and Their Applications in Gene and Cell Therapy

(2020) M. Alejandra Zeballos C. et al.   TRENDS IN BIOTECHNOLOGY

Multiple gene substitution by Target-AID base-editing technology in tomato

(2020) Johan Hunziker et al.   Scientific Reports

CRISPR/Cas9-engineered Gad1 elimination in rats leads to complex behavioral changes: implications for schizophrenia

(2020) Kazuyuki Fujihara et al.   Translational Psychiatry

Reduced RBX1 expression induces chromosome instability and promotes cellular transformation in high-grade serous ovarian cancer precursor cells

(2020) Manisha Bungsy et al.   CANCER LETTERS

RNA N6-Methyladenosine Methyltransferase METTL3 Facilitates Colorectal Cancer by Activating the m6A-GLUT1-mTORC1 Axis and Is a Therapeutic Target

(2020) Huarong Chen et al.   GASTROENTEROLOGY

Targeted mutagenesis of POLYAMINE OXIDASE 5 that negatively regulates mesocotyl elongation enables the generation of direct-seeding rice with improved grain yield

(2020) Yusong Lv et al.   Molecular Plant

A CRISPR-Cas9 repressor for epigenetic silencing of KRAS

(2020) Jingwen Liu et al.   PHARMACOLOGICAL RESEARCH

Global increase in DNA methylation during orange fruit development and ripening

(2019) Huan Huang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Progress and Challenges for Live-cell Imaging of Genomic Loci Using CRISPR-based Platforms

(2019) Xiaotian Wu et al.   GENOMICS PROTEOMICS & BIOINFORMATICS

Efficient Targeted Mutagenesis in Apple and First Time Edition of Pear Using the CRISPR-Cas9 System

(2019) Aurélie Charrier et al.   Frontiers in Plant Science

Improving plant genome editing with high-fidelity xCas9 and non-canonical PAM-targeting Cas9-NG

(2019) Zhaohui Zhong et al.   Molecular Plant

Cas9-NG greatly expands the targeting scope of genome-editing toolkit by recognizing NG and other atypical PAMs in rice

(2019) Bin Ren et al.   Molecular Plant

Programmed cell death ligand 1 disruption by clustered regularly interspaced short palindromic repeats/Cas9-genome editing promotes antitumor immunity and suppresses ovarian cancer progression

(2019) Tamaki Yahata et al.   CANCER SCIENCE

Development of drug-inducible CRISPR-Cas9 systems for large-scale functional screening

(2019) Ning Sun et al.   BMC GENOMICS

CRISPR-Cas9 mediated targeted disruption of FAD2–2 microsomal omega-6 desaturase in soybean (Glycine max.L)

(2019) Noor al Amin et al.   BMC BIOTECHNOLOGY

CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture

(2019) Kunling Chen et al.   Annual Review of Plant Biology

Mutagenesis of FAD2 genes in peanut with CRISPR/Cas9 based gene editing

(2019) Mei Yuan et al.   BMC BIOTECHNOLOGY

FLASH: a next-generation CRISPR diagnostic for multiplexed detection of antimicrobial resistance sequences

(2019) Jenai Quan et al.   NUCLEIC ACIDS RESEARCH

Engineered xCas9 and SpCas9‐NG variants broaden PAM recognition sites to generate mutations in Arabidopsis plants

(2019) Zengxiang Ge et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR /Cas9‐mediated functional recovery of the recessive rc allele to develop red rice

(2019) Yiwang Zhu et al.   PLANT BIOTECHNOLOGY JOURNAL

Elimination of Omega-1,2 Gliadins From Bread Wheat (Triticum aestivum) Flour: Effects on Immunogenic Potential and End-Use Quality

(2019) Susan B. Altenbach et al.   Frontiers in Plant Science

Increasing Cytosine Base Editing Scope and Efficiency With Engineered Cas9-PmCDA1 Fusions and the Modified sgRNA in Rice

(2019) Ying Wu et al.   Frontiers in Genetics

Outlook for coeliac disease patients: towards bread wheat with hypoimmunogenic gluten by gene editing of α- and γ-gliadin gene families

(2019) Aurélie Jouanin et al.   BMC PLANT BIOLOGY

CRISPR/Cas9 editing of three CRUCIFERIN C homoeologues alters the seed protein profile in Camelina sativa

(2019) Wendy J. Lyzenga et al.   BMC PLANT BIOLOGY

Improved drought stress tolerance in Arabidopsis by CRISPR/dCas9 fusion with a Histone AcetylTransferase

(2019) Joaquin Felipe Roca Paixão et al.   Scientific Reports

Cdk5 knocking out mediated by CRISPR-Cas9 genome editing for PD-L1 attenuation and enhanced antitumor immunity

(2019) Huan Deng et al.   Acta Pharmaceutica Sinica B

Effective editing for lysophosphatidic acid acyltransferase 2/5 in allotetraploid rapeseed (Brassica napus L.) using CRISPR-Cas9 system

(2019) Kai Zhang et al.   Biotechnology for Biofuels

CRISPR/Cas9-Based Mutagenesis of Starch Biosynthetic Genes in Sweet Potato (Ipomoea Batatas) for the Improvement of Starch Quality

(2019) Hongxia Wang et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

SHERLOCK: nucleic acid detection with CRISPR nucleases

(2019) Max J. Kellner et al.   Nature Protocols

CRISPR-Edited Stem Cells in a Patient with HIV and Acute Lymphocytic Leukemia

(2019) Lei Xu et al.   NEW ENGLAND JOURNAL OF MEDICINE

Simplified adenine base editors improve adenine base editing efficiency in rice

(2019) Kai Hua et al.   PLANT BIOTECHNOLOGY JOURNAL

Knockout of two Bna MAX 1 homologs by CRISPR /Cas9‐targeted mutagenesis improves plant architecture and increases yield in rapeseed ( Brassica napus L.)

(2019) Ming Zheng et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas9-mediated editing of CsWRKY22 reduces susceptibility to Xanthomonas citri subsp. citri in Wanjincheng orange (Citrus sinensis (L.) Osbeck)

(2019) Lijuan Wang et al.   Plant Biotechnology Reports

CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae

(2019) Young-Ah Kim et al.   Rice

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Targeted mutagenesis of BnTT8 homologs controls yellow seed coat development for effective oil production in Brassica napus L.

(2019) Yungu Zhai et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR-Cas9 disruption of PD-1 enhances activity of universal EGFRvIII CAR T cells in a preclinical model of human glioblastoma

(2019) Bryan D. Choi et al.   Journal for ImmunoTherapy of Cancer

CRISPR/Cas9-mediated targeted mutagenesis of GmLHY genes alters plant height and internode length in soybean

(2019) Qun Cheng et al.   BMC PLANT BIOLOGY

Application and prospects of CRISPR/Cas9-based methods to trace defined genomic sequences in living and fixed plant cells

(2019) Solmaz Khosravi et al.   CHROMOSOME RESEARCH

Development of CRISPR/Cas9 system in Chlorella vulgaris FSP-E to enhance lipid accumulation

(2019) Way-Rong Lin et al.   ENZYME AND MICROBIAL TECHNOLOGY

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Genome-wide identification of mildew resistance locus O (MLO) genes in tree model poplar (Populus trichocarpa): powdery mildew management in woody plants

(2018) Ertugrul Filiz et al.   EUROPEAN JOURNAL OF PLANT PATHOLOGY

Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties

(2018) Jinshan Zhang et al.   Journal of Integrative Plant Biology

Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites

(2018) Zhaohui Zhong et al.   Molecular Plant

Expanding the Scope of CRISPR/Cpf1-Mediated Genome Editing in Rice

(2018) Shaoya Li et al.   Molecular Plant

Base editing with a Cpf1–cytidine deaminase fusion

(2018) Xiaosa Li et al.   NATURE BIOTECHNOLOGY

Dynamics and function of DNA methylation in plants

(2018) Huiming Zhang et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery

(2018) Mariette Andersson et al.   PHYSIOLOGIA PLANTARUM

Analysis of the functions of TaGW2 homoeologs in wheat grain weight and protein content traits

(2018) Yi Zhang et al.   PLANT JOURNAL

CRISPR/Cas9-mediated mutagenesis of lncRNA1459 alters tomato fruit ripening

(2018) Ran Li et al.   PLANT JOURNAL

Mutagenesis of the FAE1 genes significantly changes fatty acid composition in seeds of Camelina sativa

(2018) Mehmet E. Ozseyhan et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Generation of α-solanine-free hairy roots of potato by CRISPR/Cas9 mediated genome editing of the St16DOX gene

(2018) Masaru Nakayasu et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Production of high oleic/low linoleic rice by genome editing

(2018) Kiyomi Abe et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

CRISPR/Cas9-mediated genome editing of the fatty acid desaturase 2 gene in Brassica napus

(2018) Ayako Okuzaki et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Next-generation diagnostics with CRISPR

(2018) Daniel S. Chertow   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

Highly efficient RNA-guided base editing in rabbit

(2018) Zhiquan Liu et al.   Nature Communications

The CRISPR tool kit for genome editing and beyond

(2018) Mazhar Adli   Nature Communications

Lycopene Is Enriched in Tomato Fruit by CRISPR/Cas9-Mediated Multiplex Genome Editing

(2018) Xindi Li et al.   Frontiers in Plant Science

Simultaneous Editing of Two Copies of Gh14-3-3d Confers Enhanced Transgene-Clean Plant Defense Against Verticillium dahliae in Allotetraploid Upland Cotton

(2018) Zhennan Zhang et al.   Frontiers in Plant Science

Identification and expression analysis of cytokinin metabolic genes IPTs, CYP735A and CKXs in the biofuel plant Jatropha curcas

(2018) Li Cai et al.   PeerJ

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities

(2018) Jason M Gehrke et al.   NATURE BIOTECHNOLOGY

Regulation of microRNA function in animals

(2018) Luca F. R. Gebert et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Design of a bacterial speck resistant tomato by CRISPR/Cas9-mediated editing of SlJAZ2

(2018) Andrés Ortigosa et al.   PLANT BIOTECHNOLOGY JOURNAL

PAM identification by CRISPR-Cas effector complexes: diversified mechanisms and structures

(2018) Daniel Gleditzsch et al.   RNA Biology

Engineered CRISPR-Cas9 nuclease with expanded targeting space

(2018) Hiroshi Nishimasu et al.   SCIENCE

Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato

(2018) Hiroaki Kusano et al.   Scientific Reports

Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A

(2018) Yuan Zong et al.   NATURE BIOTECHNOLOGY

Domestication of wild tomato is accelerated by genome editing

(2018) Tingdong Li et al.   NATURE BIOTECHNOLOGY

De novo domestication of wild tomato using genome editing

(2018) Agustin Zsögön et al.   NATURE BIOTECHNOLOGY

xC as9 expands the scope of genome editing with reduced efficiency in rice

(2018) Junjie Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

In vivo epigenome editing and transcriptional modulation using CRISPR technology

(2018) Cia-Hin Lau et al.   TRANSGENIC RESEARCH

Minimal PAM specificity of a highly similar SpCas9 ortholog

(2018) Pranam Chatterjee et al.   Science Advances

Genome-scale activation screen identifies a lncRNA locus regulating a gene neighbourhood

(2017) Julia Joung et al.   NATURE

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions

(2017) Y Bill Kim et al.   NATURE BIOTECHNOLOGY

Engineered Cpf1 variants with altered PAM specificities

(2017) Linyi Gao et al.   NATURE BIOTECHNOLOGY

Simultaneous Targeting of Multiple Gene Homeologs to Alter Seed Oil Production in Camelina sativa

(2017) J. A. Aznar-Moreno et al.   PLANT AND CELL PHYSIOLOGY

Targeted mutagenesis in tetraploid switchgrass (Panicum virgatum L.) using CRISPR/Cas9

(2017) Yang Liu et al.   PLANT BIOTECHNOLOGY JOURNAL

Increasing the efficiency of CRISPR-Cas9-VQR precise genome editing in rice

(2017) Xixun Hu et al.   PLANT BIOTECHNOLOGY JOURNAL

Multiplexed CRISPR/Cas9-mediated metabolic engineering of γ-aminobutyric acid levels in Solanum lycopersicum

(2017) Rui Li et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus

(2017) Aihong Peng et al.   PLANT BIOTECHNOLOGY JOURNAL

Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing

(2017) Wen Zhi Jiang et al.   PLANT BIOTECHNOLOGY JOURNAL

Low-gluten, nontransgenic wheat engineered with CRISPR/Cas9

(2017) Susana Sánchez-León et al.   PLANT BIOTECHNOLOGY JOURNAL

Generation of targeted mutant rice using a CRISPR-Cpf1 system

(2017) Rongfang Xu et al.   PLANT BIOTECHNOLOGY JOURNAL

Knocking out of carotenoid catabolic genes in rice fails to boost carotenoid accumulation, but reveals a mutation in strigolactone biosynthesis

(2017) Xiaoyu Yang et al.   PLANT CELL REPORTS

CRISPR-Cas9 Targeted Mutagenesis Leads to Simultaneous Modification of Different Homoeologous Gene Copies in Polyploid Oilseed Rape ( Brassica napus )

(2017) Janina Braatz et al.   PLANT PHYSIOLOGY

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Increased lateral root formation by CRISPR/Cas9-mediated editing of arginase genes in cotton

(2017) Yanling Wang et al.   Science China-Life Sciences

In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni

(2017) Eunji Kim et al.   Nature Communications

CRISPR/Cpf1-mediated DNA-free plant genome editing

(2017) Hyeran Kim et al.   Nature Communications

CRISPR/Cas9-mediated p53 and Pten dual mutation accelerates hepatocarcinogenesis in adult hepatitis B virus transgenic mice

(2017) Yongzhen Liu et al.   Scientific Reports

Generation of High-Amylose Rice through CRISPR/Cas9-Mediated Targeted Mutagenesis of Starch Branching Enzymes

(2017) Yongwei Sun et al.   Frontiers in Plant Science

Efficient CRISPR/Cas9 Genome Editing of Phytoene desaturase in Cassava

(2017) John Odipio et al.   Frontiers in Plant Science

The FAD2 Gene in Plants: Occurrence, Regulation, and Role

(2017) Aejaz A. Dar et al.   Frontiers in Plant Science

Lentiviral CRISPR/Cas9 vector mediated miR-21 gene editing inhibits the epithelial to mesenchymal transition in ovarian cancer cells

(2017) Wenying Huo et al.   Journal of Cancer

Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity

(2017) Alexis C. Komor et al.   Science Advances

A high-efficiency CRISPR/Cas9 system for targeted mutagenesis in Cotton (Gossypium hirsutum L.)

(2017) Chao Li et al.   Scientific Reports

Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

(2017) Vladimir Nekrasov et al.   Scientific Reports

Efficient increase of ɣ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis

(2017) Satoko Nonaka et al.   Scientific Reports

CRISPR/Cas9-induced Targeted Mutagenesis and Gene Replacement to Generate Long-shelf Life Tomato Lines

(2017) Qing-hui Yu et al.   Scientific Reports

Editing DNA Methylation in the Mammalian Genome

(2016) X. Shawn Liu et al.   CELL

Gene editing technology as an approach to the treatment of liver diseases

(2016) Rajagopal N. Aravalli et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Rapid improvement of grain weight via highly efficient CRISPR/Cas9-mediated multiplex genome editing in rice

(2016) Rongfang Xu et al.   Journal of Genetics and Genomics

Structural Basis for the Altered PAM Specificities of Engineered CRISPR-Cas9

(2016) Seiichi Hirano et al.   MOLECULAR CELL

Expanding the Range of CRISPR/Cas9 Genome Editing in Rice

(2016) Xixun Hu et al.   Molecular Plant

Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology

(2016) Jeyabharathy Chandrasekaran et al.   MOLECULAR PLANT PATHOLOGY

Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome

(2016) Maximilian Müller et al.   MOLECULAR THERAPY

New CRISPR–Cas systems from uncultivated microbes

(2016) David Burstein et al.   NATURE

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv–TET1 catalytic domain fusions

(2016) Sumiyo Morita et al.   NATURE BIOTECHNOLOGY

Repurposing the CRISPR-Cas9 system for targeted DNA methylation

(2016) Aleksandar Vojta et al.   NUCLEIC ACIDS RESEARCH

Oligonucleotide-Mediated Genome Editing Provides Precision and Function to Engineered Nucleases and Antibiotics in Plants

(2016) Noel J. Sauer et al.   PLANT PHYSIOLOGY

Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

(2016) K. Nishida et al.   SCIENCE

On the Origin of CRISPR-Cas Technology: From Prokaryotes to Mammals

(2016) Francisco J.M. Mojica et al.   TRENDS IN MICROBIOLOGY

CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci

(2016) Yi Fu et al.   Nature Communications

Reassessment of the Four Yield-related Genes Gn1a, DEP1, GS3, and IPA1 in Rice Using a CRISPR/Cas9 System

(2016) Meiru Li et al.   Frontiers in Plant Science

A Review of Auxin Response Factors (ARFs) in Plants

(2016) Si-Bei Li et al.   Frontiers in Plant Science

CRISPR-Cas9-mediated disruption of PD-1 on human T cells for adoptive cellular therapies of EBV positive gastric cancer

(2016) Shu Su et al.   OncoImmunology

Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922

(2016) Fujun Wang et al.   PLoS One

Highly specific targeted mutagenesis in plants using Staphylococcus aureus Cas9

(2016) Hidetaka Kaya et al.   Scientific Reports

CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)

(2016) Chong Ren et al.   Scientific Reports

Efficient Genome Editing in Apple Using a CRISPR/Cas9 system

(2016) Chikako Nishitani et al.   Scientific Reports

CRISPR/Cas9-mediated mutagenesis of the RIN locus that regulates tomato fruit ripening

(2015) Yasuhiro Ito et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Targeted genome modifications in soybean with CRISPR/Cas9

(2015) Thomas B Jacobs et al.   BMC BIOTECHNOLOGY

Crystal Structure of Staphylococcus aureus Cas9

(2015) Hiroshi Nishimasu et al.   CELL

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

The Next Immune-Checkpoint Inhibitors: PD-1/PD-L1 Blockade in Melanoma

(2015) Kathleen M. Mahoney et al.   CLINICAL THERAPEUTICS

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

(2015) Silvana Konermann et al.   NATURE

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

(2015) Benjamin P Kleinstiver et al.   NATURE BIOTECHNOLOGY

Functional annotation of native enhancers with a Cas9–histone demethylase fusion

(2015) Nicola A Kearns et al.   NATURE METHODS

Highly efficient Cas9-mediated transcriptional programming

(2015) Alejandro Chavez et al.   NATURE METHODS

Exploiting SNPs for biallelic CRISPR mutations in the outcrossing woody perennialPopulusreveals 4-coumarate:CoA ligase specificity and redundancy

(2015) Xiaohong Zhou et al.   NEW PHYTOLOGIST

Targeting of prolamins by RNAi in bread wheat: effectiveness of seven silencing-fragment combinations for obtaining lines devoid of coeliac disease epitopes from highly immunogenic gliadins

(2015) Francisco Barro et al.   PLANT BIOTECHNOLOGY JOURNAL

Efficient targeted mutagenesis in potato by the CRISPR/Cas9 system

(2015) Shaohui Wang et al.   PLANT CELL REPORTS

Highly efficient heritable plant genome engineering using Cas9 orthologues fromStreptococcus thermophilusandStaphylococcus aureus

(2015) Jeannette Steinert et al.   PLANT JOURNAL

Multicolor CRISPR labeling of chromosomal loci in human cells

(2015) Hanhui Ma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Making the cut

(2015) J. Travis   SCIENCE

Expression of the Rice Arginase Gene OsARG in Cotton Influences the Morphology and Nitrogen Transition of Seedlings

(2015) Zhigang Meng et al.   PLoS One

Molecular Genetic Dissection of Quantitative Trait Loci Regulating Rice Grain Size

(2014) Jianru Zuo et al.   Annual Review of Genetics

MLL3 Is a Haploinsufficient 7q Tumor Suppressor in Acute Myeloid Leukemia

(2014) Chong Chen et al.   CANCER CELL

A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging

(2014) Marvin E. Tanenbaum et al.   CELL

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

(2014) Luke A. Gilbert et al.   CELL

In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system

(2014) Danilo Maddalo et al.   NATURE

CRISPR-mediated direct mutation of cancer genes in the mouse liver

(2014) Wen Xue et al.   NATURE

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

Generating genetically modified mice using CRISPR/Cas-mediated genome engineering

(2014) Hui Yang et al.   Nature Protocols

Hairy Root Transformation Using Agrobacterium rhizogenes as a Tool for Exploring Cell Type-Specific Gene Expression and Function Using Tomato as a Model

(2014) M. Ron et al.   PLANT PHYSIOLOGY

Targeted Genome Editing of Sweet Orange Using Cas9/sgRNA

(2014) Hongge Jia et al.   PLoS One

Evaluation of sgRNA Target Sites for CRISPR-Mediated Repression of TP53

(2014) Ingrid E. B. Lawhorn et al.   PLoS One

Therapeutic Application of Endothelial Progenitor Cells for Treatment of Cardiovascular Diseases

(2014) Jian-Yong Xu et al.   Current Stem Cell Research & Therapy

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

(2014) Steven Lin et al.   eLife

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system

(2013) Albert W Cheng et al.   CELL RESEARCH

Repurposing CRISPR/Cas9 for in situ functional assays

(2013) A. Malina et al.   GENES & DEVELOPMENT

Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System

(2013) Zhen Liang et al.   Journal of Genetics and Genomics

Optical control of mammalian endogenous transcription and epigenetic states

(2013) Silvana Konermann et al.   NATURE

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

Targeted genome modification of crop plants using a CRISPR-Cas system

(2013) Qiwei Shan et al.   NATURE BIOTECHNOLOGY

Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination

(2013) Daniel J Dickinson et al.   NATURE METHODS

CRISPR RNA–guided activation of endogenous human genes

(2013) Morgan L Maeder et al.   NATURE METHODS

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

(2013) Kevin M Esvelt et al.   NATURE METHODS

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice

(2013) Wenzhi Jiang et al.   NUCLEIC ACIDS RESEARCH

Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

(2013) Z. Hou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Zinc-finger nuclease-mediated gene correction using single AAV vector transduction and enhancement by Food and Drug Administration-approved drugs

(2012) B L Ellis et al.   GENE THERAPY

Cleavage of Phage DNA by the Streptococcus thermophilus CRISPR3-Cas System

(2012) Alfonso H. Magadán et al.   PLoS One

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Meganucleases and Other Tools for Targeted Genome Engineering: Perspectives and Challenges for Gene Therapy

(2011) George Silva et al.   CURRENT GENE THERAPY

Gene-editing nucleases

(2011) Monya Baker   NATURE METHODS

Method of the Year 2011

(2011)   NATURE METHODS

Regulation of Homologous Recombination in Eukaryotes

(2010) Wolf-Dietrich Heyer et al.   Annual Review of Genetics

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

Effective shutdown in the expression of celiac disease-related wheat gliadin T-cell epitopes by RNA interference

(2010) J. Gil-Humanes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Removing celiac disease-related gluten proteins from bread wheat while retaining technological properties: a study with Chinese Spring deletion lines

(2009) Hetty C van den Broeck et al.   BMC PLANT BIOLOGY

Silencing of γ-gliadins by RNA interference (RNAi) in bread wheat

(2008) Javier Gil-Humanes et al.   JOURNAL OF CEREAL SCIENCE

Comprehensive analysis of polymorphisms throughout GAD1 gene: a family-based association study in schizophrenia

(2008) J. Du et al.   JOURNAL OF NEURAL TRANSMISSION

Arginase-Negative Mutants of Arabidopsis Exhibit Increased Nitric Oxide Signaling in Root Development

(2008) T. Flores et al.   PLANT PHYSIOLOGY