Osmolality Effects on CHO Cell Growth, Cell Volume, Antibody Productivity and Glycosylation

Media supplementation for targeted manipulation of monoclonal antibody galactosylation and fucosylation

(2020) Evan Wells et al.   BIOTECHNOLOGY AND BIOENGINEERING

Excessive Cell Growth Causes Cytoplasm Dilution And Contributes to Senescence

(2019) Gabriel E. Neurohr et al.   CELL

Model‐based optimization of antibody galactosylation in CHO cell culture

(2019) Pavlos Kotidis et al.   BIOTECHNOLOGY AND BIOENGINEERING

Process‐induced cell cycle oscillations in CHO cultures: online monitoring and model‐based investigation

(2019) Johannes Möller et al.   BIOTECHNOLOGY AND BIOENGINEERING

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

(2019) Nagesh K. Tripathi et al.   Frontiers in Bioengineering and Biotechnology

Mechanisms driving the lactate switch in Chinese hamster ovary cells

(2018) Fiona Hartley et al.   BIOTECHNOLOGY AND BIOENGINEERING

Bioprocessing of Recombinant CHO-K1, CHO-DG44, and CHO-S: CHO Expression Hosts Favor Either mAb Production or Biomass Synthesis

(2018) David Reinhart et al.   Biotechnology Journal

Transcriptome analysis of CHO cell size increase during a fed-batch process

(2018) Xiao Pan et al.   Biotechnology Journal

A Simple Method to Reduce both Lactic Acid and Ammonium Production in Industrial Animal Cell Culture

(2018) Nathaniel Freund et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Bioprocessing of Recombinant CHO-K1, CHO-DG44, and CHO-S: CHO Expression Hosts Favor Either mAb Production or Biomass Synthesis

(2018) David Reinhart et al.   Biotechnology Journal

Transcriptome analysis of CHO cell size increase during a fed-batch process

(2018) Xiao Pan et al.   Biotechnology Journal

Molecular link between glucose and glutamine consumption in cancer cells mediated by CtBP and SIRT4

(2018) Li Wang et al.   Oncogenesis

Differential gene expression of a feed-spiked super-producing CHO cell line

(2018) David Reinhart et al.   JOURNAL OF BIOTECHNOLOGY

The effect of hyperosmolality application time on production, quality, and biopotency of monoclonal antibodies produced in CHO cell fed-batch and perfusion cultures

(2018) Jinyan Qin et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Therapeutic glycoprotein production in mammalian cells

(2017) Marie-Eve Lalonde et al.   JOURNAL OF BIOTECHNOLOGY

Dynamics of immature mAb glycoform secretion during CHO cell culture: An integrated modelling framework

(2016) Ioscani Jimenez del Val et al.   Biotechnology Journal

Controlling the time evolution of mAb N-linked glycosylation, Part I: Microbioreactor experiments

(2016) Thomas K. Villiger et al.   BIOTECHNOLOGY PROGRESS

Selection of chemically defined media for CHO cell fed-batch culture processes

(2016) Xiao Pan et al.   CYTOTECHNOLOGY

Fc-galactosylation modulates antibody-dependent cellular cytotoxicity of therapeutic antibodies

(2016) Marco Thomann et al.   MOLECULAR IMMUNOLOGY

The Cost of Protein Production

(2016) Moshe Kafri et al.   Cell Reports

Dynamics of immature mAb glycoform secretion during CHO cell culture: An integrated modelling framework

(2016) Ioscani Jimenez del Val et al.   Biotechnology Journal

How does mild hypothermia affect monoclonal antibody glycosylation?

(2015) Si Nga Sou et al.   BIOTECHNOLOGY AND BIOENGINEERING

Changes in intracellular ATP-content of CHO cells as response to hyperosmolality

(2015) Jennifer Pfizenmaier et al.   BIOTECHNOLOGY PROGRESS

Increased CHO cell fed-batch monoclonal antibody production using the autophagy inhibitor 3-MA or gradually increasing osmolality

(2014) S. Soroush Nasseri et al.   BIOCHEMICAL ENGINEERING JOURNAL

A framework for the systematic design of fed-batch strategies in mammalian cell culture

(2014) Sarantos Kyriakopoulos et al.   BIOTECHNOLOGY AND BIOENGINEERING

Metabolic responses of CHO cells to limitation of key amino acids

(2014) Tiago M. Duarte et al.   BIOTECHNOLOGY AND BIOENGINEERING

Control of galactosylated glycoforms distribution in cell culture system

(2014) Neil A. McCracken et al.   BIOTECHNOLOGY PROGRESS

Effects of nutrient levels and average culture pH on the glycosylation pattern of camelid-humanized monoclonal antibody

(2014) Hengameh Aghamohseni et al.   JOURNAL OF BIOTECHNOLOGY

Modelling mammalian cellular quiescence

(2014) G. Yao   Interface Focus

The Regulation of Cell Size

(2013) Alison C. Lloyd   CELL

What determines cell size?

(2012) Wallace F Marshall et al.   BMC BIOLOGY

Mechanisms of Intracellular Scaling

(2011) Daniel L. Levy et al.   Annual Review of Cell and Developmental Biology

Exploring the effect of mild hypothermia on CHO cell productivity

(2011) Silvana Becerra et al.   BIOCHEMICAL ENGINEERING JOURNAL

Modulation of antibody galactosylation through feeding of uridine, manganese chloride, and galactose

(2011) Michael J. Gramer et al.   BIOTECHNOLOGY AND BIOENGINEERING

Observations of cell size dynamics under osmotic stress

(2011) Thomas R. Kiehl et al.   CYTOMETRY PART A

Fucose content of monoclonal antibodies can be controlled by culture medium osmolality for high antibody-dependent cellular cytotoxicity

(2011) Yoshinobu Konno et al.   CYTOTECHNOLOGY

Ectopic expression of human mTOR increases viability, robustness, cell size, proliferation, and antibody production of chinese hamster ovary cells

(2010) Imke A.J. Dreesen et al.   BIOTECHNOLOGY AND BIOENGINEERING

Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells

(2010) Xiaowei Zhang et al.   BIOTECHNOLOGY LETTERS

Intraclonal Protein Expression Heterogeneity in Recombinant CHO Cells

(2009) Warren Pilbrough et al.   PLoS One

Nutrient deprivation induces autophagy as well as apoptosis in Chinese hamster ovary cell culture

(2007) Sun Ok Hwang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Effects of Elevated pCO2 and Osmolality on Growth of CHO Cells and Production of Antibody-Fusion Protein B1: A Case Study

(2005) Marie M. Zhu et al.   BIOTECHNOLOGY PROGRESS