Controlling the time evolution of mAb N-linked glycosylation, Part I: Microbioreactor experiments

High-throughput profiling of nucleotides and nucleotide sugars to evaluate their impact on antibody N-glycosylation

(2016) Thomas K. Villiger et al.   JOURNAL OF BIOTECHNOLOGY

A framework for real-time glycosylation monitoring (RT-GM) in mammalian cell culture

(2015) Tharmala Tharmalingam et al.   BIOTECHNOLOGY AND BIOENGINEERING

How does mild hypothermia affect monoclonal antibody glycosylation?

(2015) Si Nga Sou et al.   BIOTECHNOLOGY AND BIOENGINEERING

A multi-pronged investigation into the effect of glucose starvation and culture duration on fed-batch CHO cell culture

(2015) Yuzhou Fan et al.   BIOTECHNOLOGY AND BIOENGINEERING

Recent advances in the understanding of biological implications and modulation methodologies of monoclonal antibody N-linked high mannose glycans

(2014) Helen H. Shi et al.   BIOTECHNOLOGY AND BIOENGINEERING

Identification of manipulated variables for a glycosylation control strategy

(2014) Melissa M. St. Amand et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-throughput nucleoside phosphate monitoring in mammalian cell fed-batch cultivation using quantitative matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

(2014) Robert F. Steinhoff et al.   Biotechnology Journal

High-throughput miniaturized bioreactors for cell culture process development: Reproducibility, scalability, and control

(2014) Shahid Rameez et al.   BIOTECHNOLOGY PROGRESS

Modulation of mAb quality attributes using microliter scale fed-batch cultures

(2014) Yolande Rouiller et al.   BIOTECHNOLOGY PROGRESS

Effects of nutrient levels and average culture pH on the glycosylation pattern of camelid-humanized monoclonal antibody

(2014) Hengameh Aghamohseni et al.   JOURNAL OF BIOTECHNOLOGY

Evaluating the impact of cell culture process parameters on monoclonal antibody N-glycosylation

(2014) Marija Ivarsson et al.   JOURNAL OF BIOTECHNOLOGY

Biopharmaceutical benchmarks 2014

(2014) Gary Walsh   NATURE BIOTECHNOLOGY

An optimized method for extraction and quantification of nucleotides and nucleotide sugars from mammalian cells

(2013) Ioscani Jimenez del Val et al.   ANALYTICAL BIOCHEMISTRY

An automated robotic platform for rapid profiling oligosaccharide analysis of monoclonal antibodies directly from cell culture

(2013) Margaret Doherty et al.   ANALYTICAL BIOCHEMISTRY

Self-Aliquoting Microarray Plates for Accurate Quantitative Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

(2013) Martin Pabst et al.   ANALYTICAL CHEMISTRY

Automated, High-Throughput IgG-Antibody Glycoprofiling Platform

(2013) Henning Stöckmann et al.   ANALYTICAL CHEMISTRY

The physical characterisation of a microscale parallel bioreactor platform with an industrial CHO cell line expressing an IgG4

(2013) Alvin W. Nienow et al.   BIOCHEMICAL ENGINEERING JOURNAL

CHO cell line specific prediction and control of recombinant monoclonal antibodyN-glycosylation

(2013) Rhian K. Grainger et al.   BIOTECHNOLOGY AND BIOENGINEERING

A high-throughput media design approach for high performance mammalian fed-batch cultures

(2013) Yolande Rouiller et al.   mAbs

High-throughput work flow for IgG Fc-glycosylation analysis of biotechnological samples

(2012) Dietmar Reusch et al.   ANALYTICAL BIOCHEMISTRY

Advanced microscale bioreactor system: a representative scale-down model for bench-top bioreactors

(2012) Wei-Ting Hsu et al.   CYTOTECHNOLOGY

Differences in the glycosylation of recombinant proteins expressed in HEK and CHO cells

(2012) Amelie Croset et al.   JOURNAL OF BIOTECHNOLOGY

Production, characterization and pharmacokinetic properties of antibodies with N-linked Mannose-5 glycans

(2012) Marcella Yu et al.   mAbs

Modulation of antibody galactosylation through feeding of uridine, manganese chloride, and galactose

(2011) Michael J. Gramer et al.   BIOTECHNOLOGY AND BIOENGINEERING

Effects of cell culture conditions on antibody N-linked glycosylation-what affects high mannose 5 glycoform

(2011) Efren Pacis et al.   BIOTECHNOLOGY AND BIOENGINEERING

Unique carbohydrate-carbohydrate interactions are required for high affinity binding between Fc RIII and antibodies lacking core fucose

(2011) C. Ferrara et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Towards quantitative metabolomics of mammalian cells: Development of a metabolite extraction protocol

(2010) Stefanie Dietmair et al.   ANALYTICAL BIOCHEMISTRY

Highly efficient deletion of FUT8 in CHO cell lines using zinc-finger nucleases yields cells that produce completely nonfucosylated antibodies

(2010) Laetitia Malphettes et al.   BIOTECHNOLOGY AND BIOENGINEERING

An investigation of intracellular glycosylation activities in CHO cells: Effects of nucleotide sugar precursor feeding

(2010) Niki S.C. Wong et al.   BIOTECHNOLOGY AND BIOENGINEERING

Superior In vivo Efficacy of Afucosylated Trastuzumab in the Treatment of HER2-Amplified Breast Cancer

(2010) Teemu T. Junttila et al.   CANCER RESEARCH

High productivity of human recombinant beta-interferon from a low-temperature perfusion culture

(2010) J. Rodriguez et al.   JOURNAL OF BIOTECHNOLOGY

Identification of cell culture conditions to controlN-glycosylation site-occupancy of recombinant glycoproteins expressed in CHO cells

(2009) Martin Gawlitzek et al.   BIOTECHNOLOGY AND BIOENGINEERING

Elevated Golgi pH impairs terminalN-glycosylation by inducing mislocalization of Golgi glycosyltransferases

(2009) Antti Rivinoja et al.   JOURNAL OF CELLULAR PHYSIOLOGY

N-glycan structures: recognition and processing in the ER

(2009) Markus Aebi et al.   TRENDS IN BIOCHEMICAL SCIENCES

The effects of culture conditions on the glycosylation of secreted human placental alkaline phosphatase produced in Chinese hamster ovary cells

(2008) Jong Hyun Nam et al.   BIOTECHNOLOGY AND BIOENGINEERING

Effect of culture temperature on erythropoietin production and glycosylation in a perfusion culture of recombinant CHO cells

(2008) Woo Suk Ahn et al.   BIOTECHNOLOGY AND BIOENGINEERING

Trends in Glycosylation, Glycoanalysis and Glycoengineering of Therapeutic Antibodies and Fc-Fusion Proteins

(2008) Alain Beck et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY