Broadening the scope of biocatalytic C–C bond formation

Thioesters as Acyl Donors in Biocatalytic Friedel-Crafts-type Acylation Catalyzed by Acyltransferase from Pseudomonas Protegens

(2019) Anna Żądło-Dobrowolska et al.   ChemCatChem

Biocatalytic Synthesis of α-Amino Ketones

(2019) Alison Narayan et al.   SYNLETT

Total eclipses and the heart

(2019) Luis Campos   SCIENCE

Chemoenzymatic Total Synthesis of deoxy-, epi- and Podophyllotoxin and a Biocatalytic Kinetic Resolution of Dibenzylbutyrolactones

(2019) Mattia Lazzarotto et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Biocatalytic Friedel-Crafts Alkylation Using a Promiscuous Biosynthetic Enzyme

(2019) Erica E. Schultz et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enantioselective Phenol Coupling by Laccases in the Biosynthesis of Fungal Dimeric Naphthopyrones

(2019) Sebastian Obermaier et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Biaryl-forming enzymes from Aspergilli exhibit substrate-dependent stereoselectivity

(2019) Sebastian Obermaier et al.   BIOCHEMISTRY

Broadening the Scope of Biocatalysis in Sustainable Organic Synthesis

(2019) Roger A. Sheldon et al.   ChemSusChem

Stereodivergent Intramolecular Cyclopropanation Enabled by Engineered Carbene Transferases

(2019) Ajay L. Chandgude et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed Evolution of a Cytochrome P450 Carbene Transferase for Selective Functionalization of Cyclic Compounds

(2019) Oliver F. Brandenberg et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Asymmetric Chemoenzymatic Synthesis of (−)-Podophyllotoxin and Related Aryltetralin Lignans

(2019) Jian Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enantiodivergent α-Amino C–H Fluoroalkylation Catalyzed by Engineered Cytochrome P450s

(2019) Juner Zhang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enzymatic Pictet–Spengler Reaction: Computational Study of the Mechanism and Enantioselectivity of Norcoclaurine Synthase

(2019) Xiang Sheng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structural basis for stereoselective dehydration and hydrogen-bonding catalysis by the SAM-dependent pericyclase LepI

(2019) Yujuan Cai et al.   Nature Chemistry

Machine-learning-guided directed evolution for protein engineering

(2019) Kevin K. Yang et al.   NATURE METHODS

Photoexcitation of flavoenzymes enables a stereoselective radical cyclization

(2019) Kyle F. Biegasiewicz et al.   SCIENCE

Fungal indole alkaloid biogenesis through evolution of a bifunctional reductase/Diels–Alderase

(2019) Qingyun Dan et al.   Nature Chemistry

Stereoselective Cyclopropanation of Electron-Deficient Olefins with a Cofactor Redesigned Carbene Transferase Featuring Radical Reactivity

(2019) Daniela M. Carminati et al.   ACS Catalysis

Tailoring Tryptophan Synthase TrpB for Selective Quaternary Carbon Bond Formation

(2019) Markus Dick et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Asymmetric redox-neutral radical cyclization catalysed by flavin-dependent ‘ene’-reductases

(2019) Michael J. Black et al.   Nature Chemistry

Design of an in vitro biocatalytic cascade for the manufacture of islatravir

(2019) Mark A. Huffman et al.   SCIENCE

In Vitro Reconstitution of OxyC Activity Enables Total Chemoenzymatic Syntheses of Vancomycin Aglycone Variants

(2018) Clarissa C. Forneris et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Asymmetric Reductive Carbocyclization Using Engineered Ene Reductases

(2018) Kathrin Heckenbichler et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Myoglobin-Catalyzed C−H Functionalization of Unprotected Indoles

(2018) David A. Vargas et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Formation and Cleavage of C–C Bonds by Enzymatic Oxidation–Reduction Reactions

(2018) F. Peter Guengerich et al.   CHEMICAL REVIEWS

Improved Synthesis of 4-Cyanotryptophan and Other Tryptophan Analogues in Aqueous Solvent Using Variants of TrpB from Thermotoga maritima

(2018) Christina E. Boville et al.   JOURNAL OF ORGANIC CHEMISTRY

Chemistry of a Unique Polyketide-like Synthase

(2018) Stephanie W. Chun et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

C–C bond forming radical SAM enzymes involved in the construction of carbon skeletons of cofactors and natural products

(2018) Kenichi Yokoyama et al.   NATURAL PRODUCT REPORTS

Enzymatic construction of highly strained carbocycles

(2018) Kai Chen et al.   SCIENCE

Discovery, Characterization, Engineering, and Applications of Ene-Reductases for Industrial Biocatalysis

(2018) Helen S. Toogood et al.   ACS Catalysis

Stereoselective Enzymatic Synthesis of Heteroatom-Substituted Cyclopropanes

(2018) Oliver F. Brandenberg et al.   ACS Catalysis

Diverse Engineered Heme Proteins Enable Stereodivergent Cyclopropanation of Unactivated Alkenes

(2018) Anders M. Knight et al.   ACS Central Science

Engineered biosynthesis of β-alkyl tryptophan analogs

(2018) Christina E. Boville et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Biocatalyzed C−C Bond Formation for the Production of Alkaloids

(2018) Mahesh D. Patil et al.   ChemCatChem

Highly Diastereo- and Enantioselective Synthesis of Nitrile-Substituted Cyclopropanes by Myoglobin-Mediated Carbene Transfer Catalysis

(2018) Ajay L. Chandgude et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enzymatic assembly of carbon–carbon bonds via iron-catalysed sp3 C–H functionalization

(2018) Ruijie K. Zhang et al.   NATURE

Reaction Mechanism of a Nonheme Iron Enzyme Catalyzed Oxidative Cyclization via C–C Bond Formation

(2018) Wei-chen Chang et al.   ORGANIC LETTERS

Extending the application of biocatalysis to meet the challenges of drug development

(2018) Paul N. Devine et al.   Nature Reviews Chemistry

Acyl Donors and Additives for the Biocatalytic Friedel-Crafts Acylation

(2017) Nina G. Schmidt et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Unlocking Reactivity of TrpB: A General Biocatalytic Platform for Synthesis of Tryptophan Analogues

(2017) David K. Romney et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Highly Diastereo- and Enantioselective Synthesis of Trifluoromethyl-Substituted Cyclopropanes via Myoglobin-Catalyzed Transfer of Trifluoromethylcarbene

(2017) Antonio Tinoco et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enantioselective Hydrogen Atom Transfer: Discovery of Catalytic Promiscuity in Flavin-Dependent ‘Ene’-Reductases

(2017) Braddock A. Sandoval et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

SAM-dependent enzyme-catalysed pericyclic reactions in natural product biosynthesis

(2017) Masao Ohashi et al.   NATURE

Structure and specificity of a permissive bacterial C-prenyltransferase

(2017) Sherif I Elshahawi et al.   Nature Chemical Biology

A new strategy for aromatic ring alkylation in cylindrocyclophane biosynthesis

(2017) Hitomi Nakamura et al.   Nature Chemical Biology

Enantioselective Synthesis of 4′-Ethynyl-2-fluoro-2′-deoxyadenosine (EFdA) via Enzymatic Desymmetrization

(2017) Mark McLaughlin et al.   ORGANIC LETTERS

Enzyme catalysed Pictet-Spengler formation of chiral 1,1’-disubstituted- and spiro-tetrahydroisoquinolines

(2017) Benjamin R. Lichman et al.   Nature Communications

Stereoselective Olefin Cyclopropanation under Aerobic Conditions with an Artificial Enzyme Incorporating an Iron-Chlorin e6 Cofactor

(2017) Gopeekrishnan Sreenilayam et al.   ACS Catalysis

Beyond Iron: Iridium-Containing P450 Enzymes for Selective Cyclopropanations of Structurally Diverse Alkenes

(2017) Hanna M. Key et al.   ACS Central Science

A Panel of TrpB Biocatalysts Derived from Tryptophan Synthase through the Transfer of Mutations that Mimic Allosteric Activation

(2016) Javier Murciano-Calles et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Gram-Scale Synthesis of Chiral Cyclopropane-Containing Drugs and Drug Precursors with Engineered Myoglobin Catalysts Featuring Complementary Stereoselectivity

(2016) Priyanka Bajaj et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Myoglobin-Catalyzed Olefination of Aldehydes

(2016) Vikas Tyagi et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Biocatalytic Synthesis of Allylic and Allenyl Sulfides through a Myoglobin-Catalyzed Doyle-Kirmse Reaction

(2016) Vikas Tyagi et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Recent advances on halohydrin dehalogenases—from enzyme identification to novel biocatalytic applications

(2016) Anett Schallmey et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Threonine aldolases: perspectives in engineering and screening the enzymes with enhanced substrate and stereo specificities

(2016) Kateryna Fesko   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Squalene-hopene cyclases—evolution, dynamics and catalytic scope

(2016) Per-Olof Syrén et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Recent advances of Diels–Alderases involved in natural product biosynthesis

(2016) Atsushi Minami et al.   JOURNAL OF ANTIBIOTICS

Identification of Mechanism-Based Inactivation in P450-Catalyzed Cyclopropanation Facilitates Engineering of Improved Enzymes

(2016) Hans Renata et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Abiological catalysis by artificial haem proteins containing noble metals in place of iron

(2016) Hanna M. Key et al.   NATURE

Dirigent Protein Mode of Action Revealed by the Crystal Structure of AtDIR6

(2016) Raphael Gasper et al.   PLANT PHYSIOLOGY

An artificial metalloenzyme with the kinetics of native enzymes

(2016) P. Dydio et al.   SCIENCE

Building Bridges: Biocatalytic C–C-Bond Formation toward Multifunctional Products

(2016) Nina G. Schmidt et al.   ACS Catalysis

Laccase: a multi-purpose biocatalyst at the forefront of biotechnology

(2016) Diana M. Mate et al.   Microbial Biotechnology

Expanding the Enzyme Universe: Accessing Non-Natural Reactions by Mechanism-Guided Directed Evolution

(2015) Hans Renata et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Electron transfer and reaction mechanism of laccases

(2015) Stephen M. Jones et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Modern Organic Synthesis with α-Diazocarbonyl Compounds

(2015) Alan Ford et al.   CHEMICAL REVIEWS

Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently

(2015) Andrew Currin et al.   CHEMICAL SOCIETY REVIEWS

Recent developments in enzyme promiscuity for carbon–carbon bond-forming reactions

(2015) Yufeng Miao et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Catalytic Mechanisms of Fe(II)- and 2-Oxoglutarate-dependent Oxygenases

(2015) Salette Martinez et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Natural Diels–Alderases: Elusive and Irresistable

(2015) Kimberly Klas et al.   JOURNAL OF ORGANIC CHEMISTRY

Cytochrome P450-Catalyzed Regio- and Stereoselective Phenol Coupling of Fungal Natural Products

(2015) Laura S. Mazzaferro et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Mechanistic studies on the indole prenyltransferases

(2015) Martin E. Tanner   NATURAL PRODUCT REPORTS

Methods for the directed evolution of proteins

(2015) Michael S. Packer et al.   NATURE REVIEWS GENETICS

Metabolic engineering for the production of plant isoquinoline alkaloids

(2015) Andrew Diamond et al.   PLANT BIOTECHNOLOGY JOURNAL

Directed evolution of the tryptophan synthase β-subunit for stand-alone function recapitulates allosteric activation

(2015) Andrew R. Buller et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Complete biosynthesis of opioids in yeast

(2015) S. Galanie et al.   SCIENCE

Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone

(2015) Warren Lau et al.   SCIENCE

Improved Cyclopropanation Activity of Histidine-Ligated Cytochrome P450 Enables the Enantioselective Formal Synthesis of Levomilnacipran

(2014) Z. Jane Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Terpenoid Biosynthesis Off the Beaten Track: Unconventional Cyclases and Their Impact on Biomimetic Synthesis

(2014) Martin Baunach et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Highly Diastereoselective and Enantioselective Olefin Cyclopropanation Using Engineered Myoglobin-Based Catalysts

(2014) Melanie Bordeaux et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Non-natural Olefin Cyclopropanation Catalyzed by Diverse Cytochrome P450s and Other Hemoproteins

(2014) Thomas Heel et al.   CHEMBIOCHEM

Regio- and Stereoselective Intermolecular Oxidative Phenol Coupling in Streptomyces

(2014) Andreas Präg et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A microbial biomanufacturing platform for natural and semisynthetic opioids

(2014) Kate Thodey et al.   Nature Chemical Biology

Squalene hopene cyclases are protonases for stereoselective Brønsted acid catalysis

(2014) Stephan C Hammer et al.   Nature Chemical Biology

Dirigent proteins: molecular characteristics and potential biotechnological applications

(2013) Benjamin Pickel et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Synthesis of Heterocyclic Terpenoids by Promiscuous Squalene-Hopene Cyclases

(2013) Miriam Seitz et al.   CHEMBIOCHEM

Cyclization of Squalene from Both Termini: Identification of an Onoceroid Synthase and Enzymatic Synthesis of Ambrein

(2013) Daijiro Ueda et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo

(2013) Pedro S Coelho et al.   Nature Chemical Biology

Biocatalytic retrosynthesis

(2013) Nicholas J Turner et al.   Nature Chemical Biology

Regio- and Stereoselective Oxidative Phenol Coupling inAspergillus niger

(2012) Christian Gil Girol et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Allosteric regulation of substrate channeling and catalysis in the tryptophan synthase bienzyme complex

(2012) Michael F. Dunn   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Molecular and Catalytic Properties of Monoacetylphloroglucinol Acetyltransferase fromPseudomonassp. YGJ3

(2012) Asuka HAYASHI et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

S-Adenosyl-Methionine-Dependent Methyltransferases: Highly Versatile Enzymes in Biocatalysis, Biosynthesis and Other Biotechnological Applications

(2012) Anna-Winona Struck et al.   CHEMBIOCHEM

Expanding the organic toolbox: a guide to integrating biocatalysis in synthesis

(2012) Christopher M. Clouthier et al.   CHEMICAL SOCIETY REVIEWS

Inverting the Regioselectivity of the Berberine Bridge Enzyme by Employing Customized Fluorine-Containing Substrates

(2012) Verena Resch et al.   CHEMISTRY-A EUROPEAN JOURNAL

Asymmetric bioreduction of activated alkenes to industrially relevant optically active compounds

(2012) Christoph K. Winkler et al.   JOURNAL OF BIOTECHNOLOGY

Metabolic engineering of microorganisms for the synthesis of plant natural products

(2012) Jan Marienhagen et al.   JOURNAL OF BIOTECHNOLOGY

Substrate specificity of a novel squalene-hopene cyclase from Zymomonas mobilis

(2012) Miriam Seitz et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Synthesis of highly strained terpenes by non-stop tail-to-head polycyclization

(2012) Sergey V. Pronin et al.   Nature Chemistry

Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes

(2012) P. S. Coelho et al.   SCIENCE

Stereoselective Friedel–Crafts alkylation catalyzed by squalene hopene cyclases

(2012) Stephan C. Hammer et al.   TETRAHEDRON

Activation-Independent Cyclization of Monoterpenoids

(2011) Gabriele Siedenburg et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Squalene-Hopene Cyclases

(2011) Gabriele Siedenburg et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

S-Adenosylmethionine-dependent alkylation reactions: When are radical reactions used?

(2011) Hening Lin   BIOORGANIC CHEMISTRY

C−C Bond-Forming Lyases in Organic Synthesis

(2011) Margarita Brovetto et al.   CHEMICAL REVIEWS

Novel carbon–carbon bond formations for biocatalysis

(2011) Verena Resch et al.   CURRENT OPINION IN BIOTECHNOLOGY

Engineering microbial factories for synthesis of value-added products

(2011) Jing Du et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Carbene insertion into transition metal–carbon bonds: a new tool for catalytic C–C bond formation

(2011) Nicole M. G. Franssen et al.   Catalysis Science & Technology

Enzymatic Site-Specific Functionalization of Protein Methyltransferase Substrates with Alkynes for Click Labeling

(2010) Wibke Peters et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Atroposelective Total Synthesis of Axially Chiral Biaryl Natural Products

(2010) Gerhard Bringmann et al.   CHEMICAL REVIEWS

Beyond directed evolution—semi-rational protein engineering and design

(2010) Stefan Lutz   CURRENT OPINION IN BIOTECHNOLOGY

Optimizing non-natural protein function with directed evolution

(2010) Eric M Brustad et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Mechanistic Insights on Riboflavin Synthase Inspired by Selective Binding of the 6,7-Dimethyl-8-ribityllumazine Exomethylene Anion

(2010) Ryu-Ryun Kim et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Expanding the chemical scope of RNA:methyltransferases to site-specific alkynylation of RNA for click labeling

(2010) Y. Motorin et al.   NUCLEIC ACIDS RESEARCH

Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction

(2010) J. B. Siegel et al.   SCIENCE

Biocatalytic Friedel-Crafts Alkylation Using Non-natural Cofactors

(2009) Harald Stecher et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Recent development of reactions with α-diazocarbonyl compounds as nucleophiles

(2009) Yan Zhang et al.   CHEMICAL COMMUNICATIONS

Total synthesis of chiral biaryl natural products by asymmetric biaryl coupling

(2009) Marisa C. Kozlowski et al.   CHEMICAL SOCIETY REVIEWS

Thiamin diphosphate in biological chemistry: exploitation of diverse thiamin diphosphate-dependent enzymes for asymmetric chemoenzymatic synthesis

(2009) Michael Müller et al.   FEBS Journal

Opportunities in metabolic engineering to facilitate scalable alkaloid production

(2009) Effendi Leonard et al.   Nature Chemical Biology

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Monoterpene and sesquiterpene synthases and the origin of terpene skeletal diversity in plants

(2009) Jörg Degenhardt et al.   PHYTOCHEMISTRY

Molecular Cloning and Characterization of CYP80G2, a Cytochrome P450 That Catalyzes an Intramolecular C–C Phenol Coupling of (S)-Reticuline in Magnoflorine Biosynthesis, from CulturedCoptis japonicaCells

(2008) Nobuhiro Ikezawa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Catalytic C–H functionalization by metal carbenoid and nitrenoid insertion

(2008) Huw M. L. Davies et al.   NATURE

A concerted mechanism for berberine bridge enzyme

(2008) Andreas Winkler et al.   Nature Chemical Biology

Stereoselective coupling of hemigossypol to form (+)-gossypol in moco cotton is mediated by a dirigent protein

(2008) Jinggao Liu et al.   PHYTOCHEMISTRY

Enzyme optimization: moving from blind evolution to statistical exploration of sequence–function space

(2008) Richard J. Fox et al.   TRENDS IN BIOTECHNOLOGY