Cell‐free protein synthesis: The transition from batch reactions to minimal cells and microfluidic devices

Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells

(2019) Muneaki Nakamura et al.   Nature Communications

The Application of Cell-Free Protein Synthesis in Genetic Code Expansion for Post-translational Modifications

(2019) Sumana Venkat et al.   Frontiers in Pharmacology

Cell-Free Expression and Photo-Crosslinking of the Human Neuropeptide Y2 Receptor

(2019) Lisa Maria Kögler et al.   Frontiers in Pharmacology

Application of Cell-Free Protein Synthesis for Faster Biocatalyst Development

(2019) Jascha Rolf et al.   Catalysts

Construction of Incoherent Feedforward Loop Circuits in a Cell-Free System and in Cells

(2019) Shaobin Guo et al.   ACS Synthetic Biology

Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system

(2019) Satoshi Murakami et al.   Scientific Reports

Diblock copolymers enhance folding of a mechanosensitive membrane protein during cell-free expression

(2019) Miranda L. Jacobs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

DNA-based communication in populations of synthetic protocells

(2019) Alex Joesaar et al.   Nature Nanotechnology

Cell-Free Protein Synthesis Using S30 Extracts from Escherichia coli RFzero Strains for Efficient Incorporation of Non-Natural Amino Acids into Proteins

(2019) Jiro Adachi et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Gene Expression Inside Liposomes: From Early Studies to Current Protocols

(2019) Pasquale Stano   CHEMISTRY-A EUROPEAN JOURNAL

Cell-Free Protein Synthesis: Chassis toward the Minimal Cell

(2019) Ke Yue et al.   Cells

Current developments and applications of microfluidic technology toward clinical translation of nanomedicines

(2018) Dongfei Liu et al.   ADVANCED DRUG DELIVERY REVIEWS

Review on microfluidic paper-based analytical devices towards commercialisation

(2018) Tugce Akyazi et al.   ANALYTICA CHIMICA ACTA

Reengineering Cell-Free Protein Synthesis as a Biosensor: Biosensing with Transcription, Translation, and Protein-folding

(2018) Mehran Soltani et al.   BIOCHEMICAL ENGINEERING JOURNAL

Expanding the palette of Streptomyces -based cell-free protein synthesis systems with enhanced yields

(2018) Jian Li et al.   BIOCHEMICAL ENGINEERING JOURNAL

Enhancing the efficiency of cell-free protein synthesis system by systematic titration of transcription and translation components

(2018) Yuchen Zhang et al.   BIOCHEMICAL ENGINEERING JOURNAL

Endotoxin-free E. coli -based cell-free protein synthesis: Pre-expression endotoxin removal approaches for on-demand cancer therapeutic production

(2018) Kristen M. Wilding et al.   Biotechnology Journal

Systematic optimization of cell-free synthesized human endothelin B receptor folding

(2018) Ralf-Bernhardt Rues et al.   METHODS

Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System

(2018) Ryan Marshall et al.   MOLECULAR CELL

NGR (Asn-Gly-Arg)-targeted delivery of coagulase to tumor vasculature arrests cancer cell growth

(2018) Khaled Seidi et al.   ONCOGENE

Biosensing estrogenic endocrine disruptors in human blood and urine: A RAPID cell-free protein synthesis approach

(2018) Amin S.M. Salehi et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Long-lived protein expression in hydrogel particles: towards artificial cells

(2018) Xiaoyu Zhou et al.   Chemical Science

Surface-immobilized and self-shaped DNA hydrogels and their application in biosensing

(2018) Xiaoxia Mao et al.   Chemical Science

Sculpting and fusing biomimetic vesicle networks using optical tweezers

(2018) Guido Bolognesi et al.   Nature Communications

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

(2018) Thapakorn Jaroentomeechai et al.   Nature Communications

Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids

(2018) Rey W. Martin et al.   Nature Communications

Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin

(2018) Anne Zemella et al.   Scientific Reports

Microfluidic preparation of monodisperse polymeric microspheres coated with silica nanoparticles

(2018) Dong-Yeong Kim et al.   Scientific Reports

Rapid recombinant protein expression in cell-free extracts from human blood

(2018) David Burgenson et al.   Scientific Reports

The Locational Impact of Site-Specific PEGylation: Streamlined Screening with Cell-Free Protein Expression and Coarse-Grain Simulation

(2018) Kristen M. Wilding et al.   ACS Synthetic Biology

Endotoxin-free E. coli -based cell-free protein synthesis: Pre-expression endotoxin removal approaches for on-demand cancer therapeutic production

(2018) Kristen M. Wilding et al.   Biotechnology Journal

Cell-Free Protein Synthesis From Fast-Growing Vibrio natriegens

(2018) Jurek Failmezger et al.   Frontiers in Microbiology

Microfluidic Devices for Drug Delivery Systems and Drug Screening

(2018) Samar Damiati et al.   Genes

Synthesis of Biomaterials Utilizing Microfluidic Technology

(2018) Xiaohong Wang et al.   Genes

Cell-Free Approaches in Synthetic Biology Utilizing Microfluidics

(2018) Samar Damiati et al.   Genes

Photothermal fabrication of microscale patterned DNA hydrogels

(2018) Suguru Shimomura et al.   Royal Society Open Science

Microfluidic-Assisted Fabrication of Clay Microgels for Cell-Free Protein Synthesis

(2018) Yi Jiao et al.   ACS Applied Materials & Interfaces

Cell-free protein synthesis for producing ‘difficult-to-express’ proteins

(2018) Xing Jin et al.   BIOCHEMICAL ENGINEERING JOURNAL

Continuous flow microreactor for protein PEGylation

(2018) P. Madadkar et al.   Biomicrofluidics

Integration of cell-free protein synthesis and purification in one microfluidic chip for on-demand production of recombinant protein

(2018) Xiao Xiao et al.   Biomicrofluidics

Cancer stem cells-emanated therapy resistance: Implications for liposomal drug delivery systems

(2018) Hassan Dianat-Moghadam et al.   JOURNAL OF CONTROLLED RELEASE

Supramolecular Peptide/Polymer Hybrid Hydrogels for Biomedical Applications

(2018) Elham Radvar et al.   MACROMOLECULAR BIOSCIENCE

A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers

(2018) Melissa K. Takahashi et al.   Nature Communications

Towards synthetic cells using peptide-based reaction compartments

(2018) Kilian Vogele et al.   Nature Communications

The hallmarks of living systems: towards creating artificial cells

(2018) N. Amy Yewdall et al.   Interface Focus

Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens

(2018) Benjamin J. Des Soye et al.   ACS Synthetic Biology

Proteoliposome Engineering with Cell-Free Membrane Protein Synthesis: Control of Membrane Protein Sorting into Liposomes by Chaperoning Systems

(2018) Mitsuru Ando et al.   Advanced Science

Process integration and control in continuous bioprocessing

(2018) Anurag S Rathore et al.   Current Opinion in Chemical Engineering

Signalling and differentiation in emulsion-based multi-compartmentalized in vitro gene circuits

(2018) Aurore Dupin et al.   Nature Chemistry

Programmed assembly of synthetic protocells into thermoresponsive prototissues

(2018) Pierangelo Gobbo et al.   NATURE MATERIALS

Communication and quorum sensing in non-living mimics of eukaryotic cells

(2018) Henrike Niederholtmeyer et al.   Nature Communications

A RNA producing DNA hydrogel as a platform for a high performance RNA interference system

(2018) Jaejung Song et al.   Nature Communications

Sigma Factor-Mediated Tuning of Bacterial Cell-Free Synthetic Genetic Oscillators

(2018) Maaruthy Yelleswarapu et al.   ACS Synthetic Biology

Molecular Communications in the Context of “Synthetic Cells” Research

(2018) Giordano Rampioni et al.   IEEE TRANSACTIONS ON NANOBIOSCIENCE

Cell-free microcompartmentalised transcription–translation for the prototyping of synthetic communication networks

(2018) Emilien Dubuc et al.   CURRENT OPINION IN BIOTECHNOLOGY

Microfluidic paper-based analytical devices for potential use in quantitative and direct detection of disease biomarkers in clinical analysis

(2017) Wei Yin Lim et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Passive and active droplet generation with microfluidics: a review

(2017) Pingan Zhu et al.   LAB ON A CHIP

DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis

(2017) Ping Song et al.   NANO LETTERS

Structural basis of MsbA-mediated lipopolysaccharide transport

(2017) Wei Mi et al.   NATURE

Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics

(2017) Marian Weiss et al.   NATURE MATERIALS

Synchrony and pattern formation of coupled genetic oscillators on a chip of artificial cells

(2017) Alexandra M. Tayar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell-free protein synthesis of norovirus virus-like particles

(2017) Jiayuan Sheng et al.   RSC Advances

RGD delivery of truncated coagulase to tumor vasculature affords local thrombotic activity to induce infarction of tumors in mice

(2017) Rana Jahanban-Esfahlan et al.   Scientific Reports

Gene-Mediated Chemical Communication in Synthetic Protocell Communities

(2017) T-Y. Dora Tang et al.   ACS Synthetic Biology

Synthetic Cells Synthesize Therapeutic Proteins inside Tumors

(2017) Nitzan Krinsky et al.   Advanced Healthcare Materials

Light-patterning of synthetic tissues with single droplet resolution

(2017) Michael J. Booth et al.   Scientific Reports

Cell-free protein synthesis from non-growing, stressed Escherichia coli

(2017) Jurek Failmezger et al.   Scientific Reports

Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology

(2016) Y. Elani   BIOCHEMICAL SOCIETY TRANSACTIONS

Co-translational formation and pharmacological characterization of beta1-adrenergic receptor/nanodisc complexes with different lipid environments

(2016) Ralf-Bernhardt Rues et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

DNA Microgels as a Platform for Cell-Free Protein Expression and Display

(2016) Jason S. Kahn et al.   BIOMACROMOLECULES

Synthetic Biology Provides a Toehold in the Fight against Zika

(2016) Roy A. Hall et al.   Cell Host & Microbe

On-chip automation of cell-free protein synthesis: new opportunities due to a novel reaction mode

(2016) V. Georgi et al.   LAB ON A CHIP

Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements

(2016) Richard Kelwick et al.   METABOLIC ENGINEERING

TRPV1 structures in nanodiscs reveal mechanisms of ligand and lipid action

(2016) Yuan Gao et al.   NATURE

Engineering genetic circuit interactions within and between synthetic minimal cells

(2016) Katarzyna P. Adamala et al.   Nature Chemistry

Protein synthesis directly from PCR: progress and applications of cell-free protein synthesis with linear DNA

(2016) Song-Min Schinn et al.   New Biotechnology

Droplet microfluidics: A tool for biology, chemistry and nanotechnology

(2016) Samaneh Mashaghi et al.   TRAC-TRENDS IN ANALYTICAL CHEMISTRY

Cell-Free Synthetic Biology: Engineering Beyond the Cell

(2016) Jessica G. Perez et al.   Cold Spring Harbor Perspectives in Biology

Droplet-based microfluidics for artificial cell generation: a brief review

(2016) Chiara Martino et al.   Interface Focus

Cell-Free Phospholipid Biosynthesis by Gene-Encoded Enzymes Reconstituted in Liposomes

(2016) Andrew Scott et al.   PLoS One

Liposome production by microfluidics: potential and limiting factors

(2016) Dario Carugo et al.   Scientific Reports

Cell-free methods to produce structurally intact mammalian membrane proteins

(2016) Takehiro Shinoda et al.   Scientific Reports

Controlled Transcription of Exogenous mRNA in Platelets Using Protocells

(2015) Vivienne Chan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

(2015) Amin S. M. Salehi et al.   Biotechnology Journal

Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems

(2015) Anne Zemella et al.   CHEMBIOCHEM

In vitro gene expression within membrane-free coacervate protocells

(2015) T.-Y. Dora. Tang et al.   CHEMICAL COMMUNICATIONS

The PURE system for the cell-free synthesis of membrane proteins

(2015) Yutetsu Kuruma et al.   Nature Protocols

Toward Microfluidic Reactors for Cell-Free Protein Synthesis at the Point-of-Care

(2015) Andrea C. Timm et al.   Small

Folding of newly translated membrane protein CCR5 is assisted by the chaperonin GroEL-GroES

(2015) Haixia Chi et al.   Scientific Reports

Effects of Stretching Speed on Mechanical Rupture of Phospholipid/Cholesterol Bilayers: Molecular Dynamics Simulation

(2015) Taiki Shigematsu et al.   Scientific Reports

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

(2015) Amin S. M. Salehi et al.   Biotechnology Journal

Cell-Free Synthesis of SecYEG Translocon as the Fundamental Protein Transport Machinery

(2015) Hideaki Matsubayashi et al.   ORIGINS OF LIFE AND EVOLUTION OF BIOSPHERES

Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system

(2014) Filippo Caschera et al.   BIOCHIMIE

Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System

(2014) Erik S. Zimmerman et al.   BIOCONJUGATE CHEMISTRY

Paper-Based Synthetic Gene Networks

(2014) Keith Pardee et al.   CELL

The emerging age of cell-free synthetic biology

(2014) Mark Thomas Smith et al.   FEBS LETTERS

Microcompartmentalized cell-free protein synthesis in semipermeable microcapsules composed of polyethylenimine-coated alginate

(2014) Daisuke Saeki et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation

(2014) J. Thiele et al.   LAB ON A CHIP

Multiphase Water-in-Oil Emulsion Droplets for Cell-Free Transcription–Translation

(2014) Paola Torre et al.   LANGMUIR

Evolution of enzyme catalysts caged in biomimetic gel-shell beads

(2014) Martin Fischlechner et al.   Nature Chemistry

Programmable on-chip DNA compartments as artificial cells

(2014) E. Karzbrun et al.   SCIENCE

Droplet microfluidics for single-molecule and single-cell analysis in cancer research, diagnosis and therapy

(2014) Dong-Ku Kang et al.   TRAC-TRENDS IN ANALYTICAL CHEMISTRY

Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis

(2014) Seok Hoon Hong et al.   Frontiers in Chemistry

Cell-free protein expression based on extracts from CHO cells

(2013) Andreas K. Brödel et al.   BIOTECHNOLOGY AND BIOENGINEERING

Applications of cell-free protein synthesis in synthetic biology: Interfacing bio-machinery with synthetic environments

(2013) Kyung-Ho Lee et al.   Biotechnology Journal

Engine out of the chassis: Cell-free protein synthesis and its uses

(2013) Gabriel Rosenblum et al.   FEBS LETTERS

Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate

(2013) E. Sokolova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Tissue-Like Printed Material

(2013) G. Villar et al.   SCIENCE

Interfacial assembly of protein–polymer nano-conjugates into stimulus-responsive biomimetic protocells

(2013) Xin Huang et al.   Nature Communications

Linking Genotype and Phenotype in Protein Synthesizing Liposomes with External Supply of Resources

(2013) Zohreh Nourian et al.   ACS Synthetic Biology

In Vitro Expressed GPCR Inserted in Polymersome Membranes for Ligand-Binding Studies

(2012) Sylvia May et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cell-free protein synthesis of membrane (1,3)-β-d-glucan (curdlan) synthase: Co-translational insertion in liposomes and reconstitution in nanodiscs

(2012) Agalya Periasamy et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Proteopolymersomes: In vitro production of a membrane protein in polymersome membranes

(2012) Madhavan Nallani et al.   Biointerphases

On-bead expression of recombinant proteins in an agarose gel matrix coated on a glass slide

(2012) Kyung-Ho Lee et al.   LAB ON A CHIP

In vitro selection of proteins via emulsion compartments

(2012) Wei-Cheng Lu et al.   METHODS

Cell-free protein synthesis and assembly on a biochip

(2012) Yael Heyman et al.   Nature Nanotechnology

Polymer/nucleotide droplets as bio-inspired functional micro-compartments

(2012) David S. Williams et al.   Soft Matter

Lipid–protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: Comparison with detergent micelles, bicelles and liposomes

(2011) E.N. Lyukmanova et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Coping with complexity: Machine learning optimization of cell-free protein synthesis

(2011) Filippo Caschera et al.   BIOTECHNOLOGY AND BIOENGINEERING

The incorporation of the A2 protein to produce novel Qβ virus-like particles using cell-free protein synthesis

(2011) Mark T. Smith et al.   BIOTECHNOLOGY PROGRESS

Cell-free synthesis of a functional G protein-coupled receptor complexed with nanometer scale bilayer discs

(2011) Jian-Ping Yang et al.   BMC BIOTECHNOLOGY

Cell-like systems with riboswitch controlled gene expression

(2011) Laura Martini et al.   CHEMICAL COMMUNICATIONS

Continuous protein production in nanoporous, picolitre volume containers

(2011) Piro Siuti et al.   LAB ON A CHIP

Selective Deposition and Self-Assembly of Triblock Copolymers into Matrix Arrays for Membrane Protein Production

(2011) Mirjam Andreasson-Ochsner et al.   LANGMUIR

Cell-free synthetic biology: Thinking outside the cell

(2011) C. Eric Hodgman et al.   METABOLIC ENGINEERING

High-resolution dose-response screening using droplet-based microfluidics

(2011) O. J. Miller et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vitro gene expression and enzyme catalysis in bio-inorganic protocells

(2011) Mei Li et al.   Chemical Science

An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells

(2011) Jonghyeon Shin et al.   ACS Synthetic Biology

Reconstruction of the UDP-N-acetylglucosamine biosynthetic pathway in cell-free system

(2010) Jiewen Zhou et al.   BIOTECHNOLOGY LETTERS

NMR Analyses of the Interaction between CCR5 and Its Ligand Using Functional Reconstitution of CCR5 in Lipid Bilayers

(2010) Chie Yoshiura et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Optimization and quantification of protein synthesis inside liposomes

(2009) Maryam Amidi et al.   JOURNAL OF LIPOSOME RESEARCH

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

A cell-free protein-producing gel

(2009) Nokyoung Park et al.   NATURE MATERIALS

A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells

(2008) Yutetsu Kuruma et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

An in vitro microfluidic approach to generating protein-interaction networks

(2008) Doron Gerber et al.   NATURE METHODS

Printing protein arrays from DNA arrays

(2008) Mingyue He et al.   NATURE METHODS

Cell-free synthesis and maturation of [FeFe] hydrogenases

(2007) Marcus E. Boyer et al.   BIOTECHNOLOGY AND BIOENGINEERING

Escherichia coli-based cell-free synthesis of virus-like particles

(2007) Bradley C. Bundy et al.   BIOTECHNOLOGY AND BIOENGINEERING