Cell-Free Approaches in Synthetic Biology Utilizing Microfluidics

Microfluidic Devices for Drug Delivery Systems and Drug Screening

(2018) Samar Damiati et al.   Genes

Droplet microfluidics for synthetic biology

(2017) Philip C. Gach et al.   LAB ON A CHIP

Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks

(2017) Haiyang Jia et al.   New Biotechnology

Reaction dynamics analysis of a reconstitutedEscherichia coliprotein translation system by computational modeling

(2017) Tomoaki Matsuura et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology

(2016) Y. Elani   BIOCHEMICAL SOCIETY TRANSACTIONS

Single-cell technologies in environmental omics

(2016) Rimantas Kodzius et al.   GENE

On-chip automation of cell-free protein synthesis: new opportunities due to a novel reaction mode

(2016) V. Georgi et al.   LAB ON A CHIP

Microfluidics and microbial engineering

(2016) Songzi Kou et al.   LAB ON A CHIP

Artificial cells: from basic science to applications

(2016) Can Xu et al.   Materials Today

Cell-sized asymmetric lipid vesicles facilitate the investigation of asymmetric membranes

(2016) Koki Kamiya et al.   Nature Chemistry

Design and synthesis of a minimal bacterial genome

(2016) C. A. Hutchison et al.   SCIENCE

End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis

(2016) Gregory Linshiz et al.   Journal of Biological Engineering

DNA-library assembly programmed by on-demand nano-liter droplets from a custom microfluidic chip

(2015) Uwe Tangen et al.   Biomicrofluidics

Design and construction of small perturbation mutagenesis libraries for antibody affinity maturation using massive microchip-synthesized oligonucleotides

(2015) Man Xu et al.   JOURNAL OF BIOTECHNOLOGY

Rapid modulation of droplet composition with pincer microvalves

(2015) Christopher J. Ochs et al.   LAB ON A CHIP

Marine metagenomics as a source for bioprospecting

(2015) Rimantas Kodzius et al.   Marine Genomics

Beating the odds

(2015) Uwe T Bornscheuer   Nature Chemical Biology

Synthesis and cell-free cloning of DNA libraries using programmable microfluidics

(2015) Tuval Ben Yehezkel et al.   NUCLEIC ACIDS RESEARCH

Protein synthesis in artificial cells: using compartmentalisation for spatial organisation in vesicle bioreactors

(2015) Yuval Elani et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

Effective Optimization of Antibody Affinity by Phage Display Integrated with High-Throughput DNA Synthesis and Sequencing Technologies

(2015) Dongmei Hu et al.   PLoS One

Toward Microfluidic Reactors for Cell-Free Protein Synthesis at the Point-of-Care

(2015) Andrea C. Timm et al.   Small

Inspired and stabilized by nature: ribosomal synthesis of the human voltage gated ion channel (VDAC) into 2D-protein-tethered lipid interfaces

(2015) Samar Damiati et al.   Biomaterials Science

Accessing New Chemical Entities through Microfluidic Systems

(2014) Tiago Rodrigues et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Paper-Based Synthetic Gene Networks

(2014) Keith Pardee et al.   CELL

Purified cell-free systems as standard parts for synthetic biology

(2014) Hideaki Matsubayashi et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Large-scale de novo DNA synthesis: technologies and applications

(2014) Sriram Kosuri et al.   NATURE METHODS

A Continuous-Exchange Cell-Free Protein Synthesis System Based on Extracts from Cultured Insect Cells

(2014) Marlitt Stech et al.   PLoS One

Programmable on-chip DNA compartments as artificial cells

(2014) E. Karzbrun et al.   SCIENCE

The engineering of artificial cellular nanosystems using synthetic biology approaches

(2014) Fan Wu et al.   Wiley Interdisciplinary Reviews-Nanomedicine and Nanobiotechnology

Applications of cell-free protein synthesis in synthetic biology: Interfacing bio-machinery with synthetic environments

(2013) Kyung-Ho Lee et al.   Biotechnology Journal

Semi-synthetic minimal cells: origin and recent developments

(2013) Pasquale Stano et al.   CURRENT OPINION IN BIOTECHNOLOGY

Microfluidics for biological measurements with single-molecule resolution

(2013) Aaron M Streets et al.   CURRENT OPINION IN BIOTECHNOLOGY

Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate

(2013) E. Sokolova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Expression of membrane-associated proteins within single emulsion cell facsimiles

(2012) Mayuree Chanasakulniyom et al.   ANALYST

Cell-free synthesis of cytochrome bo3 ubiquinol oxidase in artificial membranes

(2012) Ahu Arslan Yildiz et al.   ANALYTICAL BIOCHEMISTRY

In Vitro Expressed GPCR Inserted in Polymersome Membranes for Ligand-Binding Studies

(2012) Sylvia May et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

On-Chip Synthesis of Protein Microarrays from DNA Microarrays via Coupled In Vitro Transcription and Translation for Surface Plasmon Resonance Imaging Biosensor Applications

(2012) Ting H. Seefeld et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Transforming biochemical engineering with cell-free biology

(2011) James R. Swartz   AICHE JOURNAL

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Informing Biological Design by Integration of Systems and Synthetic Biology

(2011) Christina D. Smolke et al.   CELL

Microfluidic Technologies for Synthetic Biology

(2011) Parisutham Vinuselvi et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Cell-free synthetic biology: Thinking outside the cell

(2011) C. Eric Hodgman et al.   METABOLIC ENGINEERING

Inhibitory effect of common microfluidic materials on PCR outcome

(2011) Rimantas Kodzius et al.   SENSORS AND ACTUATORS B-CHEMICAL

An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells

(2011) Jonghyeon Shin et al.   ACS Synthetic Biology

A simple method of fabricating mask-free microfluidic devices for biological analysis

(2010) Xin Yi et al.   Biomicrofluidics

Miniaturized fluid array for high-throughput protein expression

(2010) Ruba Khnouf et al.   BIOTECHNOLOGY PROGRESS

Next generation microfluidic platforms for high-throughput protein biochemistry

(2010) Sebastian J Maerkl   CURRENT OPINION IN BIOTECHNOLOGY

A Highly Controllable Reconstituted Cell-Free System -a Breakthrough in Protein Synthesis Research

(2010) Hiroyuki Ohashi et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Wax-bonding 3D microfluidic chips

(2010) Xiuqing Gong et al.   LAB ON A CHIP

A microfluidic oligonucleotide synthesizer

(2010) Cheng-Chung Lee et al.   NUCLEIC ACIDS RESEARCH

Microfluidic chip: Next-generation platform for systems biology

(2009) Xiaojun Feng et al.   ANALYTICA CHIMICA ACTA

Opportunities for microfluidic technologies in synthetic biology

(2009) S. Gulati et al.   Journal of the Royal Society Interface

Advancing high-throughput gene synthesis technology

(2009) Jingdong Tian et al.   Molecular BioSystems

Microdroplet-based PCR enrichment for large-scale targeted sequencing

(2009) Ryan Tewhey et al.   NATURE BIOTECHNOLOGY

The second wave of synthetic biology: from modules to systems

(2009) Priscilla E. M. Purnick et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

RNA–protein binding kinetics in an automated microfluidic reactor

(2009) William K. Ridgeway et al.   NUCLEIC ACIDS RESEARCH

Integrated microfluidic systems for high-performance genetic analysis

(2009) Peng Liu et al.   TRENDS IN BIOTECHNOLOGY

A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells

(2008) Yutetsu Kuruma et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

An Integrated Device for Monitoring Time-Dependent in vitro Expression From Single Genes in Picolitre Droplets

(2008) Fabienne Courtois et al.   CHEMBIOCHEM

Genome-wide transcriptome analysis of 150 cell samples

(2008) Daniel Irimia et al.   Integrative Biology

Droplet microfluidics

(2008) Shia-Yen Teh et al.   LAB ON A CHIP

Microdroplets: A sea of applications?

(2008) Ansgar Huebner et al.   LAB ON A CHIP

An in vitro microfluidic approach to generating protein-interaction networks

(2008) Doron Gerber et al.   NATURE METHODS

Cell-free protein synthesis: applications in proteomics and biotechnology

(2008) Mingyue He   New Biotechnology

Ultrafast DNA sequencing on a microchip by a hybrid separation mechanism that gives 600 bases in 6.5 minutes

(2008) C. P. Fredlake et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA