Cell-free methods to produce structurally intact mammalian membrane proteins

Structural basis of human γ-secretase assembly

(2015) Linfeng Sun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The importance of ligands for G protein-coupled receptor stability

(2015) Xianjun Zhang et al.   TRENDS IN BIOCHEMICAL SCIENCES

Cell-free expression, purification and ligand-binding analysis of Drosophila melanogaster olfactory receptors DmOR67a, DmOR85b and DmORCO

(2015) Lotta Tollstoy Tegler et al.   Scientific Reports

Structural basis for the slow photocycle and late proton release in Acetabularia rhodopsin I from the marine plant Acetabularia acetabulum

(2015) Munenori Furuse et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination

(2014) Coilín Boland et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Molecular mechanism of intramembrane proteolysis by γ-secretase

(2014) Taisuke Tomita   JOURNAL OF BIOCHEMISTRY

Allosteric regulation of  -secretase activity by a phenylimidazole-type  -secretase modulator

(2014) K. Takeo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal Structure of a Claudin Provides Insight into the Architecture of Tight Junctions

(2014) H. Suzuki et al.   SCIENCE

Cell-free expression—making a mark

(2013) Frank Bernhard et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Evaluation of cell-free protein synthesis for the crystallization of membrane proteins - a case study on a member of the glutamate transporter family fromStaphylothermus marinus

(2012) Michael Jaehme et al.   FEBS Journal

Structure of a presenilin family intramembrane aspartate protease

(2012) Xiaochun Li et al.   NATURE

Facile backbone structure determination of human membrane proteins by NMR spectroscopy

(2012) Christian Klammt et al.   NATURE METHODS

Fluorescent In Situ Folding Control for Rapid Optimization of Cell-Free Membrane Protein Synthesis

(2012) Annika Müller-Lucks et al.   PLoS One

Crystal Structure ofClostridium perfringensEnterotoxin Displays Features of β-Pore-forming Toxins

(2011) Kengo Kitadokoro et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Crystal Structure of the Eukaryotic Light-Driven Proton-Pumping Rhodopsin, Acetabularia Rhodopsin II, from Marine Alga

(2011) Takashi Wada et al.   JOURNAL OF MOLECULAR BIOLOGY

Overcoming barriers to membrane protein structure determination

(2011) Roslyn M Bill et al.   NATURE BIOTECHNOLOGY

Cell-free synthesis of membrane subunits of ATP synthase in phospholipid bicelles: NMR shows subunitafold similar to the protein in the cell membrane

(2011) Eva-Maria E. Uhlemann et al.   PROTEIN SCIENCE

Benchmarking Membrane Protein Detergent Stability for Improving Throughput of High-Resolution X-ray Structures

(2011) Yo Sonoda et al.   STRUCTURE

XDS

(2010) Wolfgang Kabsch   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Crystallization of the membrane protein hVDAC1 produced in cell-free system

(2010) A. Deniaud et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Production of functional bacteriorhodopsin by anEscherichia colicell-free protein synthesis system supplemented with steroid detergent and lipid

(2009) Kazumi Shimono et al.   PROTEIN SCIENCE

Large-scale production of functional membrane proteins

(2008) F. Junge et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Quantitative analysis of sphingolipids for lipidomics using triple quadrupole and quadrupole linear ion trap mass spectrometers

(2008) Rebecca L. Shaner et al.   JOURNAL OF LIPID RESEARCH

Insertion of Membrane Proteins into Discoidal Membranes Using a Cell-Free Protein Expression Approach

(2008) Federico Katzen et al.   JOURNAL OF PROTEOME RESEARCH

A Specific Cholesterol Binding Site Is Established by the 2.8 Å Structure of the Human β2-Adrenergic Receptor

(2008) Michael A. Hanson et al.   STRUCTURE