Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis

Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system

(2014) Filippo Caschera et al.   BIOCHIMIE

Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System

(2014) Erik S. Zimmerman et al.   BIOCONJUGATE CHEMISTRY

An amino acid depleted cell-free protein synthesis system for the incorporation of non-canonical amino acid analogs into proteins

(2014) Amrita Singh-Blom et al.   JOURNAL OF BIOTECHNOLOGY

Coupling Bioorthogonal Chemistries with Artificial Metabolism: Intracellular Biosynthesis of Azidohomoalanine and Its Incorporation into Recombinant Proteins

(2014) Ying Ma et al.   MOLECULES

An Expanded Genetic Code in Mammalian Cells with a Functional Quadruplet Codon

(2013) Wei Niu et al.   ACS Chemical Biology

Recoding the Genetic Code with Selenocysteine

(2013) Markus J. Bröcker et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Facile Strategy for Selective Incorporation of Phosphoserine into Histones

(2013) Sangsik Lee et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Using E. coli-based cell-free protein synthesis to evaluate the kinetic performance of an orthogonal tRNA and aminoacyl-tRNA synthetase pair

(2013) Cem Albayrak et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

A Versatile Platform for Single- and Multiple-Unnatural Amino Acid Mutagenesis in Escherichia coli

(2013) Abhishek Chatterjee et al.   BIOCHEMISTRY

Cell-free platforms for flexible expression and screening of enzymes

(2013) Christy Catherine et al.   BIOTECHNOLOGY ADVANCES

Escherichia coli-based cell free production of flagellin and ordered flagellin display on virus-like particles

(2013) Yuan Lu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Flexizyme-Mediated Genetic Reprogramming As a Tool for Noncanonical Peptide Synthesis and Drug Discovery

(2013) Toby Passioura et al.   CHEMISTRY-A EUROPEAN JOURNAL

Cell-free translation of peptides and proteins:from high throughput screening to clinical production

(2013) Christopher J Murray et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Protein conjugation with genetically encoded unnatural amino acids

(2013) Chan Hyuk Kim et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Comparative evaluation of two cell-free protein synthesis systems derived from Escherichia coli for genetic code reprogramming

(2013) Ki Baek Lee et al.   JOURNAL OF BIOTECHNOLOGY

Reevaluation of the d-Amino Acid Compatibility with the Elongation Event in Translation

(2013) Tomoshige Fujino et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation

(2013) M. C. Jewett et al.   Molecular Systems Biology

Directed evolution of genetic parts and circuits by compartmentalized partnered replication

(2013) Jared W Ellefson et al.   NATURE BIOTECHNOLOGY

Upgrading protein synthesis for synthetic biology

(2013) Patrick O'Donoghue et al.   Nature Chemical Biology

Cell-free unnatural amino acid incorporation with alternative energy systems and linear expression templates

(2013) Prashanta Shrestha et al.   New Biotechnology

Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation

(2013) Cem Albayrak et al.   NUCLEIC ACIDS RESEARCH

Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology

(2013) James Chappell et al.   NUCLEIC ACIDS RESEARCH

Suppression of Amber Codons in Caulobacter crescentus by the Orthogonal Escherichia coli Histidyl-tRNA Synthetase/tRNAHis Pair

(2013) Jae-hyeong Ko et al.   PLoS One

Alternative fermentation conditions for improved Escherichia coli-based cell-free protein synthesis for proteins requiring supplemental components for proper synthesis

(2013) Mark Thomas Smith et al.   PROCESS BIOCHEMISTRY

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

Direct Polymerization of Proteins

(2013) Cem Albayrak et al.   ACS Synthetic Biology

Cell-free Protein Synthesis from a Release Factor 1 Deficient Escherichia coli Activates Efficient and Multiple Site-specific Nonstandard Amino Acid Incorporation

(2013) Seok Hoon Hong et al.   ACS Synthetic Biology

Genetic Incorporation of Twelve meta-Substituted Phenylalanine Derivatives Using a Single Pyrrolysyl-tRNA Synthetase Mutant

(2012) Yane-Shih Wang et al.   ACS Chemical Biology

In Vitro Selection of Anti-Akt2 Thioether-Macrocyclic Peptides Leading to Isoform-Selective Inhibitors

(2012) Yuuki Hayashi et al.   ACS Chemical Biology

Release Factor One Is Nonessential in Escherichia coli

(2012) David B. F. Johnson et al.   ACS Chemical Biology

Multiple-Site Labeling of Proteins with Unnatural Amino Acids

(2012) Karin V. Loscha et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Unnatural Amino Acid Mutagenesis of Fluorescent Proteins

(2012) Feng Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Discovery of Macrocyclic Peptides Armed with a Mechanism-Based Warhead: Isoform-Selective Inhibition of Human Deacetylase SIRT2

(2012) Jumpei Morimoto et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enhanced protein stability through minimally invasive, direct, covalent, and site-specific immobilization

(2012) Mark T. Smith et al.   BIOTECHNOLOGY PROGRESS

Recent advances in genetic code engineering in Escherichia coli

(2012) Michael Georg Hoesl et al.   CURRENT OPINION IN BIOTECHNOLOGY

Enhanced phosphoserine insertion duringEscherichia coliprotein synthesis via partial UAG codon reassignment and release factor 1 deletion

(2012) Ilka U. Heinemann et al.   FEBS LETTERS

N-Acetyl lysyl-tRNA synthetases evolved by a CcdB-based selection possessN-acetyl lysine specificity in vitro and in vivo

(2012) Takuya Umehara et al.   FEBS LETTERS

Efficient Decoding of the UAG Triplet as a Full-Fledged Sense Codon Enhances the Growth of a prfA-Deficient Strain of Escherichia coli

(2012) K. Ohtake et al.   JOURNAL OF BACTERIOLOGY

A Rationally Designed Pyrrolysyl-tRNA Synthetase Mutant with a Broad Substrate Spectrum

(2012) Yane-Shih Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Expanding the genetic code of Drosophila melanogaster

(2012) Ambra Bianco et al.   Nature Chemical Biology

Performance Analysis of Orthogonal Pairs Designed for an Expanded Eukaryotic Genetic Code

(2012) Sebastian Nehring et al.   PLoS One

Evolution of multiple, mutually orthogonal prolyl-tRNA synthetase/tRNA pairs for unnatural amino acid mutagenesis in Escherichia coli

(2012) A. Chatterjee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synthesis of site-specific antibody-drug conjugates using unnatural amino acids

(2012) J. Y. Axup et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Transforming biochemical engineering with cell-free biology

(2011) James R. Swartz   AICHE JOURNAL

Genetic-code evolution for protein synthesis with non-natural amino acids

(2011) Takahito Mukai et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

An Evolved Aminoacyl-tRNA Synthetase with Atypical Polysubstrate Specificity,

(2011) Douglas D. Young et al.   BIOCHEMISTRY

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites

(2011) David B F Johnson et al.   Nature Chemical Biology

Flexizymes for genetic code reprogramming

(2011) Yuki Goto et al.   Nature Protocols

Optimized clinical performance of growth hormone with an expanded genetic code

(2011) H. Cho et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Expanding the Genetic Code of Escherichia coli with Phosphoserine

(2011) H.-S. Park et al.   SCIENCE

An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells

(2011) Jonghyeon Shin et al.   ACS Synthetic Biology

A Facile System for Genetic Incorporation of Two Different Noncanonical Amino Acids into One Protein inEscherichia coli

(2010) Wei Wan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Using a Genetically Encoded Fluorescent Amino Acid as a Site-Specific Probe to Detect Binding of Low-Molecular-Weight Compounds

(2010) Isaac N. Ugwumba et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Site-Specific Incorporation ofp-Propargyloxyphenylalanine in a Cell-Free Environment for Direct Protein−Protein Click Conjugation

(2010) Bradley C. Bundy et al.   BIOCONJUGATE CHEMISTRY

Improving a Natural Enzyme Activity through Incorporation of Unnatural Amino Acids

(2010) Isaac N. Ugwumba et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

(2010) Heinz Neumann et al.   NATURE

Codon reassignment in the Escherichia coli genetic code

(2010) T. Mukai et al.   NUCLEIC ACIDS RESEARCH

Rational design of an orthogonal tryptophanyl nonsense suppressor tRNA

(2010) Randall A. Hughes et al.   NUCLEIC ACIDS RESEARCH

An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli

(2009) Travis S. Young et al.   JOURNAL OF MOLECULAR BIOLOGY

A Method for Genetically Installing Site-Specific Acetylation in Recombinant Histones Defines the Effects of H3 K56 Acetylation

(2009) Heinz Neumann et al.   MOLECULAR CELL

Discovery of Escherichia coli methionyl-tRNA synthetase mutants for efficient labeling of proteins with azidonorleucine in vivo

(2009) I. C. Tanrikulu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-level cell-free synthesis yields of proteins containing site-specific non-natural amino acids

(2008) Aaron R. Goerke et al.   BIOTECHNOLOGY AND BIOENGINEERING

An integrated cell-free metabolic platform for protein production and synthetic biology

(2008) Michael C Jewett et al.   Molecular Systems Biology