Journal
LAB ON A CHIP
Volume 12, Issue 9, Pages 1605-1610Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2lc21239k
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Funding
- Converging Research Center
- Intelligent Synthetic Biology Center
- National Research Foundation of Korea [2011K000841, 2011-0031946]
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A system for expression and in situ display of recombinant proteins on a microbead surface is described. Biotinylated PCR products were immobilized on microbead surfaces, which were then embedded in a gel matrix and supplied with translation machinery and substrates. Upon the incubation of the gel matrix, target proteins encoded on the bead-immobilized DNA were expressed and captured on the same bead, thus allowing bead-mediated linkage of DNA and encoded proteins. The new method combines the simplicity and convenience of solid-phase separation of genetic information with the benefits of cell-free protein synthesis, such as instant translation of genetic information, unrestricted substrate accessibility and flexible assay configuration design.
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