Application of Cell-Free Protein Synthesis for Faster Biocatalyst Development

Macromolecularly Crowded Protocells from Reversibly Shrinking Monodisperse Liposomes

(2018) Nan-Nan Deng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Pressure Test to Make 10 Molecules in 90 Days: External Evaluation of Methods to Engineer Biology

(2018) Arturo Casini et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System

(2018) Ryan Marshall et al.   MOLECULAR CELL

The E. coli S30 lysate proteome: A prototype for cell-free protein production

(2018) Daniel Foshag et al.   New Biotechnology

Evolving biocatalysis to meet bioeconomy challenges and opportunities

(2018) Alessandro Pellis et al.   New Biotechnology

Cell-free prediction of protein expression costs for growing cells

(2018) Olivier Borkowski et al.   Nature Communications

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

(2018) Thapakorn Jaroentomeechai et al.   Nature Communications

Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids

(2018) Rey W. Martin et al.   Nature Communications

Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions

(2018) Ryota Sakamoto et al.   Scientific Reports

Sequence Specific Modeling of E. coli Cell-Free Protein Synthesis

(2018) Michael Vilkhovoy et al.   ACS Synthetic Biology

Industrial Applications of Enzymes: Recent Advances, Techniques, and Outlooks

(2018) Jordan Chapman et al.   Catalysts

Microfluidic Devices for Drug Delivery Systems and Drug Screening

(2018) Samar Damiati et al.   Genes

Cell-Free Approaches in Synthetic Biology Utilizing Microfluidics

(2018) Samar Damiati et al.   Genes

Microfluidic-Assisted Fabrication of Clay Microgels for Cell-Free Protein Synthesis

(2018) Yi Jiao et al.   ACS Applied Materials & Interfaces

Cell-free protein synthesis for producing ‘difficult-to-express’ proteins

(2018) Xing Jin et al.   BIOCHEMICAL ENGINEERING JOURNAL

Cell-free protein synthesis: Recent advances in bacterial extract sources and expanded applications

(2018) Wan-Qiu Liu et al.   BIOCHEMICAL ENGINEERING JOURNAL

Expanding biological applications using cell-free metabolic engineering: An overview

(2018) James R. Swartz   METABOLIC ENGINEERING

Cell-free pipeline for discovery of thermotolerant xylanases and endo -1,4-β-glucanases

(2017) Dejan Gagoski et al.   JOURNAL OF BIOTECHNOLOGY

Identification of Antibody Targets for Tuberculosis Serology using High-Density Nucleic Acid Programmable Protein Arrays

(2017) Lusheng Song et al.   MOLECULAR & CELLULAR PROTEOMICS

Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks

(2017) Haiyang Jia et al.   New Biotechnology

Confronting the catalytic dark matter encoded by sequenced genomes

(2017) Kenneth W. Ellens et al.   NUCLEIC ACIDS RESEARCH

Codon usage regulates protein structure and function by affecting translation elongation speed in Drosophila cells

(2017) Fangzhou Zhao et al.   NUCLEIC ACIDS RESEARCH

The fourth wave of biocatalysis is approaching

(2017) Uwe T. Bornscheuer   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES

Biocatalysis in the Pharmaceutical Industry: The Need for Speed

(2017) Matthew D. Truppo   ACS Medicinal Chemistry Letters

Experimentally Validated Model Enables Debottlenecking of in Vitro Protein Synthesis and Identifies a Control Shift under in Vivo Conditions

(2017) Alexander Nieß et al.   ACS Synthetic Biology

Cofactor NAD(P)H Regeneration Inspired by Heterogeneous Pathways

(2017) Xiaodong Wang et al.   Chem

Activity screening of environmental metagenomic libraries reveals novel carboxylesterase families

(2017) Ana Popovic et al.   Scientific Reports

On-chip automation of cell-free protein synthesis: new opportunities due to a novel reaction mode

(2016) V. Georgi et al.   LAB ON A CHIP

A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery

(2016) Ashty S. Karim et al.   METABOLIC ENGINEERING

Bioinformatics for the synthetic biology of natural products: integrating across the Design–Build–Test cycle

(2016) Pablo Carbonell et al.   NATURAL PRODUCT REPORTS

Applications of Multiphasic Microreactors for Biocatalytic Reactions

(2016) Rohan Karande et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Cell-free protein engineering of Old Yellow Enzyme 1 from Saccharomyces pastorianus

(2016) Leann T. Quertinmont et al.   TETRAHEDRON

Protein Synthesis in Coupled and Uncoupled Cell-Free Prokaryotic Gene Expression Systems

(2016) Maike M. K. Hansen et al.   ACS Synthetic Biology

The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology

(2016) Jonathan Garamella et al.   ACS Synthetic Biology

Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis

(2016) Quentin M. Dudley et al.   ACS Synthetic Biology

Biochemical Preparation of Cell Extract for Cell-Free Protein Synthesis without Physical Disruption

(2016) Kei Fujiwara et al.   PLoS One

A Simple and Rapid Method for Preparing a Cell-Free Bacterial Lysate for Protein Synthesis

(2016) Nitzan Krinsky et al.   PLoS One

Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks

(2015) John A. Gerlt et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Implementing bacterial acid resistance into cell-free protein synthesis for buffer-free expression and screening of enzymes

(2015) Ho-Cheol Kim et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

(2015) Amin S. M. Salehi et al.   Biotechnology Journal

A cell-free expression and purification process for rapid production of protein biologics

(2015) Challise J. Sullivan et al.   Biotechnology Journal

Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems

(2015) Anne Zemella et al.   CHEMBIOCHEM

RApid Parallel Protein EvaluatoR (RAPPER), from gene to enzyme function in one day

(2015) L. T. Quertinmont et al.   CHEMICAL COMMUNICATIONS

Optimization of the Electroformation of Giant Unilamellar Vesicles (GUVs) with Unsaturated Phospholipids

(2015) Marie Breton et al.   JOURNAL OF MEMBRANE BIOLOGY

Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction

(2015) Brian R. Fritz et al.   NUCLEIC ACIDS RESEARCH

Protein synthesis in artificial cells: using compartmentalisation for spatial organisation in vesicle bioreactors

(2015) Yuval Elani et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

Toward Microfluidic Reactors for Cell-Free Protein Synthesis at the Point-of-Care

(2015) Andrea C. Timm et al.   Small

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

(2015) Amin S. M. Salehi et al.   Biotechnology Journal

A cell-free expression and purification process for rapid production of protein biologics

(2015) Challise J. Sullivan et al.   Biotechnology Journal

Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system

(2014) Filippo Caschera et al.   BIOCHIMIE

Cell-free metabolic engineering: Biomanufacturing beyond the cell

(2014) Quentin M. Dudley et al.   Biotechnology Journal

Integration of biological parts toward the synthesis of a minimal cell

(2014) Filippo Caschera et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes

(2014) Rita Sachse et al.   FEBS LETTERS

Design of Experiments (DoE) and Process Optimization. A Review of Recent Publications

(2014) Steven A. Weissman et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Application of environmental and economic metrics to guide the development of biocatalytic processes

(2014) Joana Lima-Ramos et al.   Green Processing and Synthesis

Cell-free synthesis of membrane proteins: Tailored cell models out of microsomes

(2013) Susanne F. Fenz et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Cell-free platforms for flexible expression and screening of enzymes

(2013) Christy Catherine et al.   BIOTECHNOLOGY ADVANCES

Cell-free protein expression based on extracts from CHO cells

(2013) Andreas K. Brödel et al.   BIOTECHNOLOGY AND BIOENGINEERING

Applications of cell-free protein synthesis in synthetic biology: Interfacing bio-machinery with synthetic environments

(2013) Kyung-Ho Lee et al.   Biotechnology Journal

Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis

(2013) Jennifer A. Schoborg et al.   Biotechnology Journal

Engine out of the chassis: Cell-free protein synthesis and its uses

(2013) Gabriel Rosenblum et al.   FEBS LETTERS

Optimised ‘on demand’ protein arraying from DNA by cell free expression with the ‘DNA to Protein Array’ (DAPA) technology

(2013) Ronny Schmidt et al.   Journal of Proteomics

Molecular crowding shapes gene expression in synthetic cellular nanosystems

(2013) Cheemeng Tan et al.   Nature Nanotechnology

In vitro evolution of  -hemolysin using a liposome display

(2013) S. Fujii et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell-free Protein Synthesis in a Microchamber Revealed the Presence of an Optimum Compartment Volume for High-order Reactions

(2013) Taiji Okano et al.   ACS Synthetic Biology

Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System

(2013) Zachary Z. Sun et al.   ACS Synthetic Biology

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

(2013) Zachary Z. Sun et al.   Jove-Journal of Visualized Experiments

A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution

(2012) Ali Fallah-Araghi et al.   LAB ON A CHIP

Global analysis of chaperone effects using a reconstituted cell-free translation system

(2012) T. Niwa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

TECHNOLOGY PROSPECTING ON ENZYMES: APPLICATION, MARKETING AND ENGINEERING

(2012) Shuang Li et al.   Computational and Structural Biotechnology Journal

The Enzyme Function Initiative

(2011) John A. Gerlt et al.   BIOCHEMISTRY

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

Simplifying and streamlining Escherichia coli-based cell-free protein synthesis

(2011) William C. Yang et al.   BIOTECHNOLOGY PROGRESS

Cell-Free Protein Expression under Macromolecular Crowding Conditions

(2011) Xumeng Ge et al.   PLoS One

A Highly Controllable Reconstituted Cell-Free System -a Breakthrough in Protein Synthesis Research

(2010) Hiroyuki Ohashi et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Miniaturization in Biocatalysis

(2010) Pedro Fernandes   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Rational improvement of cell-free protein synthesis

(2010) Anders Pedersen et al.   New Biotechnology

Application of mechanistic models to fermentation and biocatalysis for next-generation processes

(2010) Krist V. Gernaey et al.   TRENDS IN BIOTECHNOLOGY

Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70

(2010) Jonghyeon Shin et al.   Journal of Biological Engineering

Cell-free protein synthesis energized by slowly-metabolized maltodextrin

(2009) Yiran Wang et al.   BMC BIOTECHNOLOGY

Multi-step microfluidic droplet processing: kinetic analysis of an in vitro translated enzyme

(2009) Linas Mazutis et al.   LAB ON A CHIP

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

Engineered enzymes for chemical production

(2008) Stephan Luetz et al.   BIOTECHNOLOGY AND BIOENGINEERING

In situ synthesis of protein arrays

(2008) Mingyue He et al.   CURRENT OPINION IN BIOTECHNOLOGY

Printing protein arrays from DNA arrays

(2008) Mingyue He et al.   NATURE METHODS

An Economical Method for Cell-Free Protein Synthesis using Glucose and Nucleoside Monophosphates

(2005) Kara A. Calhoun et al.   BIOTECHNOLOGY PROGRESS