Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems

A technique to increase protein yield in a rabbit reticulocyte lysate translation system

(2016) Maria Anastasina et al.   BIOTECHNIQUES

A versatile coupled cell-free transcription-translation system based on tobacco BY-2 cell lysates

(2015) Matthias Buntru et al.   BIOTECHNOLOGY AND BIOENGINEERING

Membrane protein production inEscherichia colicell-free lysates

(2015) Erik Henrich et al.   FEBS LETTERS

Cotranslational incorporation of non-standard amino acids using cell-free protein synthesis

(2015) Robert B. Quast et al.   FEBS LETTERS

Automated production of functional membrane proteins using eukaryotic cell-free translation systems

(2015) Robert B. Quast et al.   JOURNAL OF BIOTECHNOLOGY

Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay

(2015) Hiroyuki Takeda et al.   Scientific Reports

Synthesis and site-directed fluorescence labeling of azido proteins using eukaryotic cell-free orthogonal translation systems

(2014) Robert B. Quast et al.   ANALYTICAL BIOCHEMISTRY

Bio-ethanol production through simultaneous saccharification and fermentation using an encapsulated reconstituted cell-free enzyme system

(2014) Muhammad Wajid Ullah et al.   BIOCHEMICAL ENGINEERING JOURNAL

Evaluating fermentation effects on cell growth and crude extract metabolic activity for improved yeast cell-free protein synthesis

(2014) Alaksh Choudhury et al.   BIOCHEMICAL ENGINEERING JOURNAL

Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system

(2014) Srujan Kumar Dondapati et al.   BIOSENSORS & BIOELECTRONICS

Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system

(2014) Matthias Buntru et al.   BMC BIOTECHNOLOGY

Cell-free eukaryotic systems for the production, engineering, and modification of scFv antibody fragments

(2014) Marlitt Stech et al.   ENGINEERING IN LIFE SCIENCES

Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes

(2014) Rita Sachse et al.   FEBS LETTERS

Wheat germ systems for cell-free protein expression

(2014) Matthias Harbers   FEBS LETTERS

Characterizing IGR IRES-mediated translation initiation for use in yeast cell-free protein synthesis

(2014) C. Eric Hodgman et al.   New Biotechnology

Conformation transitions of eukaryotic polyribosomes during multi-round translation

(2014) Zhanna A. Afonina et al.   NUCLEIC ACIDS RESEARCH

A Continuous-Exchange Cell-Free Protein Synthesis System Based on Extracts from Cultured Insect Cells

(2014) Marlitt Stech et al.   PLoS One

Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis

(2014) Seok Hoon Hong et al.   Frontiers in Chemistry

Cell-free synthesis of membrane proteins: Tailored cell models out of microsomes

(2013) Susanne F. Fenz et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Cell-free protein expression based on extracts from CHO cells

(2013) Andreas K. Brödel et al.   BIOTECHNOLOGY AND BIOENGINEERING

Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis

(2013) C. Eric Hodgman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis

(2013) Jennifer A. Schoborg et al.   Biotechnology Journal

Cell-free translation of peptides and proteins:from high throughput screening to clinical production

(2013) Christopher J Murray et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Application of wheat germ cell-free protein expression system for novel malaria vaccine candidate discovery

(2013) Thangavelu U Arumugam et al.   Expert Review of Vaccines

Engine out of the chassis: Cell-free protein synthesis and its uses

(2013) Gabriel Rosenblum et al.   FEBS LETTERS

Upgrading protein synthesis for synthetic biology

(2013) Patrick O'Donoghue et al.   Nature Chemical Biology

Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation

(2013) Cem Albayrak et al.   NUCLEIC ACIDS RESEARCH

IRES-Mediated Translation of Membrane Proteins and Glycoproteins in Eukaryotic Cell-Free Systems

(2013) Andreas K. Brödel et al.   PLoS One

Alternative fermentation conditions for improved Escherichia coli-based cell-free protein synthesis for proteins requiring supplemental components for proper synthesis

(2013) Mark Thomas Smith et al.   PROCESS BIOCHEMISTRY

Production of Recombinant Therapeutic Proteins in Human Cells: Current Achievements and Future Perspectives

(2013) Virgínia Picanco-Castro et al.   PROTEIN AND PEPTIDE LETTERS

A miRNA-responsive cell-free translation system facilitates isolation of hepatitis C virus miRNP complexes

(2013) S. S. Bradrick et al.   RNA

Cell-free synthesis of functional thermostable direct hemolysins of Vibrio parahaemolyticus

(2013) Silke Bechlars et al.   TOXICON

Development of oligomannose-coated liposome-based nasal vaccine against human parainfluenza virus type 3

(2013) Kyosuke Senchi et al.   Frontiers in Microbiology

Synthesis of membrane proteins in eukaryotic cell-free systems

(2012) Rita Sachse et al.   ENGINEERING IN LIFE SCIENCES

The protease-activated receptor 1 possesses a functional and cleavable signal peptide which is necessary for receptor expression

(2012) Dimitris E. Zampatis et al.   FEBS LETTERS

Functional evaluation of candidate ice structuring proteins using cell-free expression systems

(2012) A.K. Brödel et al.   JOURNAL OF BIOTECHNOLOGY

Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system

(2012) Marlitt Stech et al.   JOURNAL OF BIOTECHNOLOGY

A “Tag-and-Modify” Approach to Site-Selective Protein Modification

(2011) Justin M. Chalker et al.   ACCOUNTS OF CHEMICAL RESEARCH

CHO cells in biotechnology for production of recombinant proteins: current state and further potential

(2011) Jee Yon Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Mammalian cell protein expression for biopharmaceutical production

(2011) Jianwei Zhu   BIOTECHNOLOGY ADVANCES

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins

(2011) Cassandra Guarino et al.   GLYCOBIOLOGY

An efficient factor-depleted mammalian in vitro translation system

(2011) Aurélie M Rakotondrafara et al.   Nature Protocols

Optimized clinical performance of growth hormone with an expanded genetic code

(2011) H. Cho et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

N-terminally truncated GADD34 proteins are convenient translation enhancers in a human cell-derived in vitro protein synthesis system

(2010) Satoshi Mikami et al.   BIOTECHNOLOGY LETTERS

Giant Vesicles: Preparations and Applications

(2010) Peter Walde et al.   CHEMBIOCHEM

Synthesis of proteins with defined posttranslational modifications using the genetic noncanonical amino acid incorporation approach

(2010) Wenshe R. Liu et al.   Molecular BioSystems

Practical cell-free protein synthesis system using purified wheat embryos

(2010) Kazuyuki Takai et al.   Nature Protocols

Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System

(2010) Oleksiy Kovtun et al.   PLoS One

Multiply mutated Gaussia luciferases provide prolonged and intense bioluminescence

(2009) John P. Welsh et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

How Does a Scanning Ribosomal Particle Move along the 5′-Untranslated Region of Eukaryotic mRNA? Brownian Ratchet Model

(2009) Alexander S. Spirin   BIOCHEMISTRY

Bridging IRES elements in mRNAs to the eukaryotic translation apparatus

(2009) Kerry D. Fitzgerald et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

Regulation of Translation Initiation in Eukaryotes: Mechanisms and Biological Targets

(2009) Nahum Sonenberg et al.   CELL

Protein Incorporation in Giant Lipid Vesicles under Physiological Conditions

(2009) Paige M. Shaklee et al.   CHEMBIOCHEM

Position-Specific Incorporation of Fluorescent Non-natural Amino Acids into Maltose-Binding Protein for Detection of Ligand Binding by FRET and Fluorescence Quenching

(2009) Issei Iijima et al.   CHEMBIOCHEM

Membrane protein assembly into Nanodiscs

(2009) Timothy H. Bayburt et al.   FEBS LETTERS

Recombinant Protein Expression in Leishmania tarentolae

(2009) Giancarlo Basile et al.   MOLECULAR BIOTECHNOLOGY

A Method for Genetically Installing Site-Specific Acetylation in Recombinant Histones Defines the Effects of H3 K56 Acetylation

(2009) Heinz Neumann et al.   MOLECULAR CELL

Species-independent translational leaders facilitate cell-free expression

(2009) Sergei Mureev et al.   NATURE BIOTECHNOLOGY

Translation initiation factors are not required for Dicistroviridae IRES function in vivo

(2009) N. Deniz et al.   RNA

Replication of Tomato bushy stunt virus RNA in a plant in vitro system

(2009) Torsten Gursinsky et al.   VIROLOGY

Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex

(2008) Michael A. Goren et al.   PROTEIN EXPRESSION AND PURIFICATION

A human cell-derived in vitro coupled transcription/translation system optimized for production of recombinant proteins

(2008) Satoshi Mikami et al.   PROTEIN EXPRESSION AND PURIFICATION