Engine out of the chassis: Cell-free protein synthesis and its uses

Quantifying Elongation Rhythm during Full-Length Protein Synthesis

(2013) Gabriel Rosenblum et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation

(2013) M. C. Jewett et al.   Molecular Systems Biology

Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation

(2013) Cem Albayrak et al.   NUCLEIC ACIDS RESEARCH

Artificial Environments for the Co-Translational Stabilization of Cell-Free Expressed Proteins

(2013) Lei Kai et al.   PLoS One

Real-time assay for testing components of protein synthesis

(2012) Gabriel Rosenblum et al.   NUCLEIC ACIDS RESEARCH

Reconstitution of translation from Thermus thermophilus reveals a minimal set of components sufficient for protein synthesis at high temperatures and functional conservation of modern and ancient translation components

(2012) Ying Zhou et al.   NUCLEIC ACIDS RESEARCH

5'-UTR RNA G-quadruplexes: translation regulation and targeting

(2012) A. Bugaut et al.   NUCLEIC ACIDS RESEARCH

Global analysis of chaperone effects using a reconstituted cell-free translation system

(2012) T. Niwa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Localization of BiP to translating ribosomes increases soluble accumulation of secreted eukaryotic proteins in an Escherichia coli cell-free system

(2011) John P. Welsh et al.   BIOTECHNOLOGY AND BIOENGINEERING

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective 15N-labelling and production of perdeuterated proteins in H2O

(2011) Xun-Cheng Su et al.   JOURNAL OF BIOMOLECULAR NMR

Amphiphilic Polysaccharide Nanogels as Artificial Chaperones in Cell-Free Protein Synthesis

(2011) Yoshihiro Sasaki et al.   MACROMOLECULAR BIOSCIENCE

RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites

(2011) David B F Johnson et al.   Nature Chemical Biology

A PCR-directed cell-free approach to optimize protein expression using diverse fusion tags

(2011) Andrew V. Kralicek et al.   PROTEIN EXPRESSION AND PURIFICATION

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

The key function of a conserved and modified rRNA residue in the ribosomal response to the nascent peptide

(2010) Nora Vázquez-Laslop et al.   EMBO JOURNAL

In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme

(2010) Haruichi Asahara et al.   NUCLEIC ACIDS RESEARCH

Site-specific labeling of Saccharomyces cerevisiae ribosomes for single-molecule manipulations

(2010) Alexey Petrov et al.   NUCLEIC ACIDS RESEARCH

Study of messenger RNA inactivation and protein degradation in an Escherichia coli cell-free expression system

(2010) Jonghyeon Shin et al.   Journal of Biological Engineering

Profiling of gene-dependent translational progress in cell-free protein synthesis by real-space imaging

(2009) Eriko Mikamo-Satoh et al.   ANALYTICAL BIOCHEMISTRY

Fast Biosynthesis of GFP Molecules: A Single-Molecule Fluorescence Study

(2009) Alexandros Katranidis et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Do all modifications benefit all tRNAs?

(2009) Eric M. Phizicky et al.   FEBS LETTERS

Species-independent translational leaders facilitate cell-free expression

(2009) Sergei Mureev et al.   NATURE BIOTECHNOLOGY

Optimization of the hybridization-based method for purification of thermostable tRNAs in the presence of tetraalkylammonium salts

(2009) Takashi Yokogawa et al.   NUCLEIC ACIDS RESEARCH

Continuous-exchange cell-free protein synthesis using PCR-generated DNA and an RNase E-deficient extract

(2008) Soo Youn Jun et al.   BIOTECHNIQUES

Incorporation of Pseudouridine Into mRNA Yields Superior Nonimmunogenic Vector With Increased Translational Capacity and Biological Stability

(2008) Katalin Karikó et al.   MOLECULAR THERAPY

A human cell-derived in vitro coupled transcription/translation system optimized for production of recombinant proteins

(2008) Satoshi Mikami et al.   PROTEIN EXPRESSION AND PURIFICATION