Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

LyophilizedEscherichia coli-based cell-free systems for robust, high-density, long-term storage

(2016) Mark Thomas Smith et al.   BIOTECHNIQUES

Onconase induces autophagy sensitizing pancreatic cancer cells to gemcitabine and activates Akt/mTOR pathway in a ROS-dependent manner

(2015) Claudia Fiorini et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Peering into the pharmaceutical “pipeline”: Investigational drugs, clinical trials, and industry priorities

(2015) Jill A. Fisher et al.   SOCIAL SCIENCE & MEDICINE

2013 Statistics for Global Prescription Medications: CNS Therapeutics Maintain a Leading Position among Small Molecule Therapeutics

(2014) Craig W. Lindsley   ACS Chemical Neuroscience

Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system

(2014) Filippo Caschera et al.   BIOCHIMIE

The emerging age of cell-free synthetic biology

(2014) Mark Thomas Smith et al.   FEBS LETTERS

Biopharmaceutical benchmarks 2014

(2014) Gary Walsh   NATURE BIOTECHNOLOGY

Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis

(2013) Jennifer A. Schoborg et al.   Biotechnology Journal

Cell-free unnatural amino acid incorporation with alternative energy systems and linear expression templates

(2013) Prashanta Shrestha et al.   New Biotechnology

Alternative fermentation conditions for improved Escherichia coli-based cell-free protein synthesis for proteins requiring supplemental components for proper synthesis

(2013) Mark Thomas Smith et al.   PROCESS BIOCHEMISTRY

Synthesis of membrane proteins in eukaryotic cell-free systems

(2012) Rita Sachse et al.   ENGINEERING IN LIFE SCIENCES

Transforming biochemical engineering with cell-free biology

(2011) James R. Swartz   AICHE JOURNAL

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

The incorporation of the A2 protein to produce novel Qβ virus-like particles using cell-free protein synthesis

(2011) Mark T. Smith et al.   BIOTECHNOLOGY PROGRESS

A cytotoxic ribonuclease reduces the expression level of P-glycoprotein in multidrug-resistant cell lines

(2011) Jessica Castro et al.   INVESTIGATIONAL NEW DRUGS

Cell-free synthesis and multifold screening of Candida antarctica lipase B (CalB) variants after combinatorial mutagenesis of hot spots

(2010) Chang-Gil Park et al.   BIOTECHNOLOGY PROGRESS

Secretory expression of glycosylated and aglycosylated mutein of onconase fromPichia pastorisusing different secretion signals and their purification and characterization

(2009) Hong Liang Zhao et al.   FEMS YEAST RESEARCH

Interaction of onconase with the human ribonuclease inhibitor protein

(2008) Rebecca F. Turcotte et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

High yield cell-free production of integral membrane proteins without refolding or detergents

(2008) Jessica J. Wuu et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Onconase and Amphinase, the Antitumor Ribonucleases from Rana pipiens Oocytes

(2008) W. Ardelt et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Cell-free synthesis and maturation of [FeFe] hydrogenases

(2007) Marcus E. Boyer et al.   BIOTECHNOLOGY AND BIOENGINEERING