Single-domain antibodies for biomedical applications

The Shark Strikes Twice: Hypervariable Loop 2 of Shark IgNAR Antibody Variable Domains and Its Potential to Function as an Autonomous Paratope

(2015) Stefan Zielonka et al.   MARINE BIOTECHNOLOGY

Engineered Fc based antibody domains and fragments as novel scaffolds

(2014) Tianlei Ying et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Mutational approaches to improve the biophysical properties of human single-domain antibodies

(2014) Dae Young Kim et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Antibody–drug conjugates—A new wave of cancer drugs

(2014) Hervé Bouchard et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

GSK2374697, a Novel Albumin-Binding Domain Antibody (AlbudAb), Extends Systemic Exposure of Exendin-4: First Study in Humans—PK/PD and Safety

(2014) R L O’Connor-Semmes et al.   CLINICAL PHARMACOLOGY & THERAPEUTICS

Shark variable new antigen receptor biologics – a novel technology platform for therapeutic drug development

(2014) Marina Kovaleva et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Bispecific T-cell engagers for cancer immunotherapy

(2014) Amelia M Huehls et al.   IMMUNOLOGY AND CELL BIOLOGY

Shark Attack: High affinity binding proteins derived from shark vNAR domains by stepwise in vitro affinity maturation

(2014) Stefan Zielonka et al.   JOURNAL OF BIOTECHNOLOGY

Streptavidin-biotin-based directional double Nanobody sandwich ELISA for clinical rapid and sensitive detection of influenza H5N1

(2014) Min Zhu et al.   Journal of Translational Medicine

Structural insights and biomedical potential of IgNAR scaffolds from sharks

(2014) Stefan Zielonka et al.   mAbs

Antibodies to watch in 2015

(2014) Janice M Reichert   mAbs

The structural analysis of shark IgNAR antibodies reveals evolutionary principles of immunoglobulins

(2014) M. J. Feige et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

VHH (nanobody) directed against human glycophorin A: A tool for autologous red cell agglutination assays

(2013) Ibrahim Habib et al.   ANALYTICAL BIOCHEMISTRY

Anti-tumor necrosis factor VNAR single domains reduce lethality and regulate underlying inflammatory response in a murine model of endotoxic shock

(2013) Rafael Bojalil et al.   BMC IMMUNOLOGY

Anticalins: Exploiting a non-Ig scaffold with hypervariable loops for the engineering of binding proteins

(2013) A. Richter et al.   FEBS LETTERS

Nanobodies as novel agents for disease diagnosis and therapy

(2013) Christina G. Siontorou   International Journal of Nanomedicine

Atypical Antigen Recognition Mode of a Shark Immunoglobulin New Antigen Receptor (IgNAR) Variable Domain Characterized by Humanization and Structural Analysis

(2013) Oleg V. Kovalenko et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Engineered Soluble Monomeric IgG1 CH3 Domain

(2013) Tianlei Ying et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Llama-derived Single Variable Domains (Nanobodies) Directed against Chemokine Receptor CXCR7 Reduce Head and Neck Cancer Cell Growthin Vivo

(2013) David Maussang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Autoantibodies to Variable Heavy (VH) Chain Ig Sequences in Humans Impact the Safety and Clinical Pharmacology of a VH Domain Antibody Antagonist of TNF-α Receptor 1

(2013) M. C. Holland et al.   JOURNAL OF CLINICAL IMMUNOLOGY

A Monovalent Anti-Human CD28 Domain Antibody Antagonist: Preclinical Efficacy and Safety

(2013) S. J. Suchard et al.   JOURNAL OF IMMUNOLOGY

Exceptionally Potent and Broadly Cross-Reactive, Bispecific Multivalent HIV-1 Inhibitors Based on Single Human CD4 and Antibody Domains

(2013) W. Chen et al.   JOURNAL OF VIROLOGY

Which are the antibodies to watch in 2013?

(2013) Janice M. Reichert   mAbs

Antibody light chain variable domains and their biophysically improved versions for human immunotherapy

(2013) Dae Young Kim et al.   mAbs

High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli

(2013) Kristof Zarschler et al.   Microbial Cell Factories

A Human Single-Domain Antibody Elicits Potent Antitumor Activity by Targeting an Epitope in Mesothelin Close to the Cancer Cell Surface

(2013) Z. Tang et al.   MOLECULAR CANCER THERAPEUTICS

Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma

(2013) M. Feng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Evaluation of efficacy and safety of the anti-VWF Nanobody ALX-0681 in a preclinical baboon model of acquired thrombotic thrombocytopenic purpura

(2012) F. Callewaert et al.   BLOOD

Nanobody-Based Targeting of the Macrophage Mannose Receptor for EffectiveIn VivoImaging of Tumor-Associated Macrophages

(2012) Kiavash Movahedi et al.   CANCER RESEARCH

Surface display of a single-domain antibody library on Gram-positive bacteria

(2012) Filippa Fleetwood et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Monoclonal antibody therapeutics: history and future

(2012) Nicholas APS Buss et al.   CURRENT OPINION IN PHARMACOLOGY

Natural and Engineered Cystine Knot Miniproteins for Diagnostic and Therapeutic Applications

(2012) Harald Kolmar   CURRENT PHARMACEUTICAL DESIGN

Immunocytokines: a novel class of potent armed antibodies

(2012) Nadine Pasche et al.   DRUG DISCOVERY TODAY

Variable domain antibodies specific for viral hemorrhagic septicemia virus (VHSV) selected from a randomized IgNAR phage display library

(2012) Maki Ohtani et al.   FISH & SHELLFISH IMMUNOLOGY

Antibody–drug conjugates: Basic concepts, examples and future perspectives

(2012) Giulio Casi et al.   JOURNAL OF CONTROLLED RELEASE

Pharmacokinetics of engineered human monomeric and dimeric CH2 domains

(2012) Kurt R. Gehlsen et al.   mAbs

Improving the pharmacokinetic properties of biologics by fusion to an anti-HSA shark VNAR domain

(2012) Mischa R. Müller et al.   mAbs

Marketed therapeutic antibodies compendium

(2012) Janice M. Reichert   mAbs

Human Monoclonal Antibodies Targeting Nonoverlapping Epitopes on Insulin-like Growth Factor II as a Novel Type of Candidate Cancer Therapeutics

(2012) W. Chen et al.   MOLECULAR CANCER THERAPEUTICS

What's fueling the biotech engine—2011 to 2012

(2012) Saurabh Aggarwal   NATURE BIOTECHNOLOGY

Bispecific Engineered Antibody Domains (Nanoantibodies) That Interact Noncompetitively with an HIV-1 Neutralizing Epitope and FcRn

(2012) Rui Gong et al.   PLoS One

The TITAN trial – Assessing the efficacy and safety of an anti-von Willebrand factor Nanobody in patients with acquired thrombotic thrombocytopenic purpura

(2012) Josefin-Beate Holz   TRANSFUSION AND APHERESIS SCIENCE

Immuno-imaging using nanobodies

(2011) Ilse Vaneycken et al.   CURRENT OPINION IN BIOTECHNOLOGY

DARPins and other repeat protein scaffolds: advances in engineering and applications

(2011) Ykelien L Boersma et al.   CURRENT OPINION IN BIOTECHNOLOGY

Shortened Engineered Human Antibody CH2 Domains

(2011) Rui Gong et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Clinical impact of serum proteins on drug delivery

(2011) Felix Kratz et al.   JOURNAL OF CONTROLLED RELEASE

Crystal Structure of the Amyloid-  p3 Fragment Provides a Model for Oligomer Formation in Alzheimer's Disease

(2011) V. A. Streltsov et al.   JOURNAL OF NEUROSCIENCE

Isolation and characterisation of Ebolavirus-specific recombinant antibody fragments from murine and shark immune libraries

(2011) Sarah A. Goodchild et al.   MOLECULAR IMMUNOLOGY

What's fueling the biotech engine—2010 to 2011

(2011) Saurabh Aggarwal   NATURE BIOTECHNOLOGY

Targeting the hepatitis B virus precore antigen with a novel IgNAR single variable domain intrabody

(2011) Renae Walsh et al.   VIROLOGY

A Case Of Convergence: Why Did a Simple Alternative to Canonical Antibodies Arise in Sharks and Camels?

(2011) Martin F. Flajnik et al.   PLOS BIOLOGY

In Vitro Analysis and In Vivo Tumor Targeting of a Humanized, Grafted Nanobody in Mice Using Pinhole SPECT/Micro-CT

(2010) I. Vaneycken et al.   JOURNAL OF NUCLEAR MEDICINE

Engineered CH2 domains (nanoantibodies)

(2010) Dimiter S Dimitrov   mAbs

Have we overestimated the benefit of human(ized) antibodies?

(2010) Daniel R. Getts et al.   mAbs

Anti-TNFα domain antibody construct CEP-37247

(2010) Robert D. Gay et al.   mAbs

Cell culture processes for monoclonal antibody production

(2010) Feng Li et al.   mAbs

The immunogenicity of humanized and fully human antibodies

(2010) Fiona A. Harding et al.   mAbs

CXCR4 nanobodies (VHH-based single variable domains) potently inhibit chemotaxis and HIV-1 replication and mobilize stem cells

(2010) S. Jahnichen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Introducing antigen-binding sites in structural loops of immunoglobulin constant domains: Fc fragments with engineered HER2/neu-binding sites and antibody properties

(2010) G. Wozniak-Knopp et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A large library based on a novel (CH2) scaffold: Identification of HIV-1 inhibitors

(2009) Xiaodong Xiao et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris

(2009) Stefan Ryckaert et al.   JOURNAL OF BIOTECHNOLOGY

Single domain antibodies: promising experimental and therapeutic tools in infection and immunity

(2009) Janusz Wesolowski et al.   MEDICAL MICROBIOLOGY AND IMMUNOLOGY

Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library

(2009) Ana Monegal et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Immunogenicity negatively influences the outcome of adalimumab treatment in Crohn’s disease

(2008) R. L. WEST et al.   ALIMENTARY PHARMACOLOGY & THERAPEUTICS

Formation of antibodies against infliximab and adalimumab strongly correlates with functional drug levels and clinical responses in rheumatoid arthritis

(2008) T R D J Radstake et al.   ANNALS OF THE RHEUMATIC DISEASES

General Strategy to Humanize a Camelid Single-domain Antibody and Identification of a Universal Humanized Nanobody Scaffold

(2008) Cécile Vincke et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Thermodynamically Stable Aggregation-Resistant Antibody Domains through Directed Evolution

(2008) Kristoffer Famm et al.   JOURNAL OF MOLECULAR BIOLOGY

SPECT Imaging with 99mTc-Labeled EGFR-Specific Nanobody for In Vivo Monitoring of EGFR Expression

(2008) Lieven Huang et al.   MOLECULAR IMAGING AND BIOLOGY

Shark IgNAR antibody mimotopes target a murine immunoglobulin through extended CDR3 loop structures

(2007) David P. Simmons et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS