RNA Dependent RNA Polymerases: Insights from Structure, Function and Evolution

Near-Atomic Resolution Structure Determination of a Cypovirus Capsid and Polymerase Complex Using Cryo-EM at 200kV

(2017) Xiaowu Li et al.   JOURNAL OF MOLECULAR BIOLOGY

Initiation, Elongation, and Realignment during Influenza Virus mRNA Synthesis

(2017) Aartjan J. W. te Velthuis et al.   JOURNAL OF VIROLOGY

Crystal Structure and Thermostability Characterization of Enterovirus D68 3D pol

(2017) Chunnian Wang et al.   JOURNAL OF VIROLOGY

Norovirus RNA-dependent RNA polymerase: A computational study of metal-binding preferences

(2017) Md Munan Shaik et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

A structural view of the RNA-dependent RNA polymerases from the Flavivirus genus

(2017) Guoliang Lu et al.   VIRUS RESEARCH

Structure and Function of Caliciviral RNA Polymerases

(2017) Ji-Hye Lee et al.   Viruses-Basel

Dengue Virus Non-Structural Protein 5

(2017) et al.   Viruses-Basel

Crystal structure of Zika virus NS5 RNA-dependent RNA polymerase

(2017) Andre S. Godoy et al.   Nature Communications

Host Factors in the Infection Cycle of Bamboo mosaic virus

(2017) Ying-Ping Huang et al.   Frontiers in Microbiology

Crystal structure of full-lengthZika virusNS5 protein reveals a conformation similar toJapanese encephalitis virusNS5

(2017) Anup K. Upadhyay et al.   Acta Crystallographica Section F-Structural Biology Communications

Approved Antiviral Drugs over the Past 50 Years

(2016) Erik De Clercq et al.   CLINICAL MICROBIOLOGY REVIEWS

Towards a structural understanding of RNA synthesis by negative strand RNA viral polymerases

(2016) Juan Reguera et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Thumb Site 2 Inhibitors of Hepatitis C Viral RNA-dependent RNA Polymerase Allosterically Block the Transition from Initiation to Elongation

(2016) Jiawen Li et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Design of a Genetically Stable High Fidelity Coxsackievirus B3 Polymerase That Attenuates Virus Growthin Vivo

(2016) Seth McDonald et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Discovery of Potent Non-Nucleoside Inhibitors of Dengue Viral RNA-Dependent RNA Polymerase from a Fragment Hit Using Structure-Based Drug Design

(2016) Fumiaki Yokokawa et al.   JOURNAL OF MEDICINAL CHEMISTRY

The Influenza Virus Polymerase Complex: An Update on Its Structure, Functions, and Significance for Antiviral Drug Design

(2016) Annelies Stevaert et al.   MEDICINAL RESEARCH REVIEWS

An updated evolutionary study of Flaviviridae NS3 helicase and NS5 RNA-dependent RNA polymerase reveals novel invariable motifs as potential pharmacological targets

(2016) Louis Papageorgiou et al.   Molecular BioSystems

Structural basis of an essential interaction between influenza polymerase and Pol II CTD

(2016) Maria Lukarska et al.   NATURE

Structural basis of viral RNA-dependent RNA polymerase catalysis and translocation

(2016) Bo Shu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell-Free and Cell-Based Approaches to Explore the Roles of Host Membranes and Lipids in the Formation of Viral Replication Compartment Induced by Tombusviruses

(2016) Peter Nagy et al.   Viruses-Basel

Dengue Virus Nonstructural Protein 5 (NS5) Assembles into a Dimer with a Unique Methyltransferase and Polymerase Interface

(2016) Valerie J. Klema et al.   PLoS Pathogens

Initiation of RNA Polymerization and Polymerase Encapsidation by a Small dsRNA Virus

(2016) Aaron M. Collier et al.   PLoS Pathogens

The role of the priming loop in influenza A virus RNA synthesis

(2016) Aartjan J. W. te Velthuis et al.   Nature Microbiology

The dengue virus NS5 protein as a target for drug discovery

(2015) Siew Pheng Lim et al.   ANTIVIRAL RESEARCH

Structural Insights into Bunyavirus Replication and Its Regulation by the vRNA Promoter

(2015) Piotr Gerlach et al.   CELL

Structure of the L Protein of Vesicular Stomatitis Virus from Electron Cryomicroscopy

(2015) Bo Liang et al.   CELL

A Structural Overview of RNA-Dependent RNA Polymerases from the Flaviviridae Family

(2015) Jiqin Wu et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Flavivirus RNA synthesis in vitro

(2015) Radhakrishnan Padmanabhan et al.   METHODS

Crystal structure of the RNA-dependent RNA polymerase from influenza C virus

(2015) Narin Hengrung et al.   NATURE

Structural basis for RNA-genome recognition during bacteriophage Qβ replication

(2015) Heidi Gytz et al.   NUCLEIC ACIDS RESEARCH

Structural Analysis of Monomeric RNA-Dependent Polymerases: Evolutionary and Therapeutic Implications

(2015) Rodrigo Jácome et al.   PLoS One

Structure and inhibition of EV-D68, a virus that causes respiratory illness in children

(2015) Y. Liu et al.   SCIENCE

Structural basis for RNA replication by the hepatitis C virus polymerase

(2015) T. C. Appleby et al.   SCIENCE

Origins and evolution of viruses of eukaryotes: The ultimate modularity

(2015) Eugene V. Koonin et al.   VIROLOGY

RNA-Dependent RNA Polymerases of Picornaviruses: From the Structure to Regulatory Mechanisms

(2015) Cristina Ferrer-Orta et al.   Viruses-Basel

Inhibitors of the Hepatitis C Virus Polymerase; Mode of Action and Resistance

(2015) Auda Eltahla et al.   Viruses-Basel

The RNA Template Channel of the RNA-Dependent RNA Polymerase as a Target for Development of Antiviral Therapy of Multiple Genera within a Virus Family

(2015) Lonneke van der Linden et al.   PLoS Pathogens

The Structure of the RNA-Dependent RNA Polymerase of a Permutotetravirus Suggests a Link between Primer-Dependent and Primer-Independent Polymerases

(2015) Diego S. Ferrero et al.   PLoS Pathogens

Common and unique features of viral RNA-dependent polymerases

(2014) Aartjan J. W. te Velthuis   CELLULAR AND MOLECULAR LIFE SCIENCES

HCV-targeted Antivirals: Current Status and Future Challenges

(2014) Sandra Gemma et al.   CURRENT PHARMACEUTICAL DESIGN

The RNA-dependent RNA polymerase of the influenza A virus

(2014) Thomas M Stubbs et al.   Future Virology

Structure-Function Relationships Underlying the Replication Fidelity of Viral RNA-Dependent RNA Polymerases

(2014) Grace Campagnola et al.   JOURNAL OF VIROLOGY

The Crystal Structure of a Cardiovirus RNA-Dependent RNA Polymerase Reveals an Unusual Conformation of the Polymerase Active Site

(2014) L. Vives-Adrian et al.   JOURNAL OF VIROLOGY

Automated Structural Comparisons Clarify the Phylogeny of the Right-Hand-Shaped Polymerases

(2014) Heli A. M. Mönttinen et al.   MOLECULAR BIOLOGY AND EVOLUTION

Structural insight into cap-snatching and RNA synthesis by influenza polymerase

(2014) Stefan Reich et al.   NATURE

Molecular insights into replication initiation by Qβ replicase using ribosomal protein S1

(2014) Daijiro Takeshita et al.   NUCLEIC ACIDS RESEARCH

Structural Bases of Norovirus RNA Dependent RNA Polymerase Inhibition by Novel Suramin-Related Compounds

(2014) Romina Croci et al.   PLoS One

Evolution of Tertiary Structure of Viral RNA Dependent Polymerases

(2014) Jiří Černý et al.   PLoS One

Membranous Replication Factories Induced by Plus-Strand RNA Viruses

(2014) Inés Romero-Brey et al.   Viruses-Basel

Host and viral RNA-binding proteins involved in membrane targeting, replication and intercellular movement of plant RNA virus genomes

(2014) Kiwamu Hyodo et al.   Frontiers in Plant Science

Structure of a backtracked state reveals conformational changes similar to the state following nucleotide incorporation in human norovirus polymerase

(2014) Dmitry Zamyatkin et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Role of Motif B Loop in Allosteric Regulation of RNA-Dependent RNA Polymerization Activity

(2013) Damià Garriga et al.   JOURNAL OF MOLECULAR BIOLOGY

Conformational Flexibility of the Dengue Virus RNA-Dependent RNA Polymerase Revealed by a Complex with an Inhibitor

(2013) C. G. Noble et al.   JOURNAL OF VIROLOGY

RNA-dependent RNA polymerase of Japanese encephalitis virus binds the initiator nucleotide GTP to form a mechanistically important pre-initiation state

(2013) Parag Surana et al.   NUCLEIC ACIDS RESEARCH

Structures of Coxsackievirus, Rhinovirus, and Poliovirus Polymerase Elongation Complexes Solved by Engineering RNA Mediated Crystal Contacts

(2013) Peng Gong et al.   PLoS One

Structure of the RNA-directed RNA Polymerase from the cystovirus ϕ12

(2013) Zhen Ren et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Crystal Structure of the Full-Length Japanese Encephalitis Virus NS5 Reveals a Conserved Methyltransferase-Polymerase Interface

(2013) Guoliang Lu et al.   PLoS Pathogens

A holistic evolutionary and structural study offlaviviridaeprovides insights into the function and inhibition of HCV helicase

(2013) Dimitrios Vlachakis et al.   PeerJ

Antivirals: Past, present and future

(2012) Erik De Clercq   BIOCHEMICAL PHARMACOLOGY

Expanding networks of RNA virus evolution

(2012) Eugene V Koonin et al.   BMC BIOLOGY

Structure-Based Inhibition of Norovirus RNA-Dependent RNA Polymerases

(2012) Eloise Mastrangelo et al.   JOURNAL OF MOLECULAR BIOLOGY

Structure-Activity Relationships in the Development of Allosteric Hepatitis C Virus RNA-Dependent RNA Polymerase Inhibitors: Ten Years of Research

(2012) Romain Haudecoeur et al.   MEDICINAL RESEARCH REVIEWS

Structural insights into the coupling of virion assembly and rotavirus replication

(2012) Shane D. Trask et al.   NATURE REVIEWS MICROBIOLOGY

Molecular basis for RNA polymerization by Qβ replicase

(2012) Daijiro Takeshita et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Highly similar structural frames link the template tunnel and NTP entry tunnel to the exterior surface in RNA-dependent RNA polymerases

(2012) Dorothy M. Lang et al.   NUCLEIC ACIDS RESEARCH

Functional Analysis of Conserved Motifs in Influenza Virus PB1 Protein

(2012) Caroline Chu et al.   PLoS One

Motif D of Viral RNA-Dependent RNA Polymerases Determines Efficiency and Fidelity of Nucleotide Addition

(2012) Xiaorong Yang et al.   STRUCTURE

Mutational analysis of residues involved in nucleotide and divalent cation stabilization in the rotavirus RNA-dependent RNA polymerase catalytic pocket

(2012) Kristen M. Ogden et al.   VIROLOGY

Nucleoside, Nucleotide, and Non-Nucleoside Inhibitors of Hepatitis C Virus NS5B RNA-Dependent RNA-Polymerase

(2011) Michael J. Sofia et al.   JOURNAL OF MEDICINAL CHEMISTRY

Noncatalytic Ions Direct the RNA-Dependent RNA Polymerase of Bacterial Double-Stranded RNA Virus  6 from De Novo Initiation to Elongation

(2011) S. Wright et al.   JOURNAL OF VIROLOGY

The N-Terminus of the RNA Polymerase from Infectious Pancreatic Necrosis Virus Is the Determinant of Genome Attachment

(2011) Stephen C. Graham et al.   PLoS Pathogens

Strategies for development of dengue virus inhibitors

(2010) Christian G. Noble et al.   ANTIVIRAL RESEARCH

Mechanism of Intraparticle Synthesis of the Rotavirus Double-stranded RNA Genome

(2010) Kristen M. Guglielmi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure of the Q  replicase, an RNA-dependent RNA polymerase consisting of viral and host proteins

(2010) R. T. Kidmose et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Assembly of a functional Machupo virus polymerase complex

(2010) P. J. Kranzusch et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase

(2010) P. Gong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The ins and outs of four-tunneled Reoviridae RNA-dependent RNA polymerases

(2009) Sarah M McDonald et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

RNA-dependent RNA polymerases from Flaviviridae

(2009) Kyung H Choi et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Binding of 2′-Amino-2′-Deoxycytidine-5′-Triphosphate to Norovirus Polymerase Induces Rearrangement of the Active Site

(2009) Dmitry F. Zamyatkin et al.   JOURNAL OF MOLECULAR BIOLOGY

Does the evolution of viral polymerases reflect the origin and evolution of viruses?

(2009) Mart Krupovič et al.   NATURE REVIEWS MICROBIOLOGY

Nucleic acid polymerases use a general acid for nucleotidyl transfer

(2009) Christian Castro et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

PROSITE, a protein domain database for functional characterization and annotation

(2009) Christian J. A. Sigrist et al.   NUCLEIC ACIDS RESEARCH

Structural Insights into Mechanisms of Catalysis and Inhibition in Norwalk Virus Polymerase

(2008) Dmitry F. Zamyatkin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Crystal Structure of Coxsackievirus B3 RNA-Dependent RNA Polymerase in Complex with Its Protein Primer VPg Confirms the Existence of a Second VPg Binding Site on Picornaviridae Polymerases

(2008) A. Gruez et al.   JOURNAL OF VIROLOGY

Modification of intracellular membrane structures for virus replication

(2008) Sven Miller et al.   NATURE REVIEWS MICROBIOLOGY

Mechanism for Coordinated RNA Packaging and Genome Replication by Rotavirus Polymerase VP1

(2008) Xiaohui Lu et al.   STRUCTURE

Role of tRNA-like structures in controlling plant virus replication

(2008) Theo W. Dreher   VIRUS RESEARCH