Discerning conformational dynamics and binding kinetics of GPCRs by 19F NMR

Nanobody GPS by PCS: An Efficient New NMR Analysis Method for G Protein Coupled Receptors and Other Large Proteins

(2022) Feng-Jie Wu et al.   Journal of the American Chemical Society

19F NMR as a tool in chemical biology

(2021) Diana Gimenez et al.   Beilstein Journal of Organic Chemistry

Delineating the conformational landscape of the adenosine A2A receptor during G protein coupling

(2021) Shuya Kate Huang et al.   CELL

Advances in the study of GPCRs by 19F NMR

(2021) Louis-Philippe Picard et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Molecular insights into the biased signaling mechanism of the μ-opioid receptor

(2021) Xiaojing Cong et al.   MOLECULAR CELL

A Genetically Encoded F-19 NMR Probe Reveals the Allosteric Modulation Mechanism of Cannabinoid Receptor 1

(2021) Xiaoyan Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structural equilibrium underlying ligand-dependent activation of β2-adrenoreceptor

(2020) Shunsuke Imai et al.   Nature Chemical Biology

Probing the correlation between ligand efficacy and conformational diversity at the α1A-adrenoreceptor reveals allosteric coupling of its microswitches

(2020) Feng-Jie Wu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Activation of adenosine A2A receptor by lipids from docosahexaenoic acid revealed by NMR

(2020) Takuya Mizumura et al.   Science Advances

The precious fluorine on the ring: fluorine NMR for biological systems

(2020) Andras Boeszoermenyi et al.   JOURNAL OF BIOMOLECULAR NMR

Use of paramagnetic 19F NMR to monitor domain movement in a glutamate transporter homolog

(2020) Yun Huang et al.   Nature Chemical Biology

Genetic Encoding of para-Pentafluorosulfanyl Phenylalanine: A Highly Hydrophobic and Strongly Electronegative Group for Stable Protein Interactions

(2020) Haocheng Qianzhu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Aromatic 19F-13C TROSY: a background-free approach to probe biomolecular structure, function, and dynamics

(2019) Andras Boeszoermenyi et al.   NATURE METHODS

Simultaneous Ligand and Receptor Tracking through NMR Spectroscopy Enabled by Distinct 19F Labels

(2019) Jeffrey R. Simmons et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Conformational transitions of a neurotensin receptor 1–Gi1 complex

(2019) Hideaki E. Kato et al.   NATURE

GPCRomics: An Approach to Discover GPCR Drug Targets

(2019) Paul A. Insel et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Methyl TROSY spectroscopy: A versatile NMR approach to study challenging biological systems

(2019) Stefan Schütz et al.   PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Optimization and 13 CH 3 methionine labeling of a signaling competent neurotensin receptor 1 variant for NMR studies

(2018) Fabian Bumbak et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Allosteric Coupling of Drug Binding and Intracellular Signaling in the A 2A Adenosine Receptor

(2018) Matthew T. Eddy et al.   CELL

A genetically encoded 19F NMR probe for lysine acetylation

(2018) Feng Zhang et al.   CHEMICAL COMMUNICATIONS

Mixed Fluorotryptophan Substitutions at the Same Residue Expand the Versatility of 19 F Protein NMR Spectroscopy

(2018) Calem Kenward et al.   CHEMISTRY-A EUROPEAN JOURNAL

Deuteration and selective labeling of alanine methyl groups of β2-adrenergic receptor expressed in a baculovirus-insect cell expression system

(2018) Yutaka Kofuku et al.   JOURNAL OF BIOMOLECULAR NMR

Extrinsic Tryptophans as NMR Probes of Allosteric Coupling in Membrane Proteins: Application to the A2A Adenosine Receptor

(2018) Matthew T. Eddy et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Current NMR Techniques for Structure-Based Drug Discovery

(2018) Toshihiko Sugiki et al.   MOLECULES

Structure and dynamics of GPCR signaling complexes

(2018) Daniel Hilger et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure and dynamics of GPCR signaling complexes

(2018) Daniel Hilger et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Methyl-Based NMR Spectroscopy Methods for Uncovering Structural Dynamics in Large Proteins and Protein Complexes

(2018) Zachary K. Boswell et al.   BIOCHEMISTRY

Ligand-Based Fluorine NMR Screening: Principles and Applications in Drug Discovery Projects

(2018) Claudio Dalvit et al.   JOURNAL OF MEDICINAL CHEMISTRY

A2A adenosine receptor functional states characterized by 19F-NMR

(2018) Lukas Sušac et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Trends in GPCR drug discovery: new agents, targets and indications

(2017) Alexander S. Hauser et al.   NATURE REVIEWS DRUG DISCOVERY

Ligand modulation of sidechain dynamics in a wild-type human GPCR

(2017) Lindsay D Clark et al.   eLife

Applications of 19F-NMR in Fragment-Based Drug Discovery

(2016) Raymond Norton et al.   MOLECULES

Activation of the A2A adenosine G-protein-coupled receptor by conformational selection

(2016) Libin Ye et al.   NATURE

Enzyme Dynamics from NMR Spectroscopy

(2015) Arthur G. Palmer   ACCOUNTS OF CHEMICAL RESEARCH

Structural Insights into the Dynamic Process of β 2 -Adrenergic Receptor Signaling

(2015) Aashish Manglik et al.   CELL

Propagation of conformational changes during μ-opioid receptor activation

(2015) Rémy Sounier et al.   NATURE

Single-particle cryo-electron microscopy of macromolecular complexes

(2015) Georgios Skiniotis et al.   Microscopy

Fluorinated Aromatic Amino Acids Are Sensitive 19F NMR Probes for Bromodomain-Ligand Interactions

(2014) Neeraj K. Mishra et al.   ACS Chemical Biology

Functional Dynamics of Deuterated β2-Adrenergic Receptor in Lipid Bilayers Revealed by NMR Spectroscopy

(2014) Yutaka Kofuku et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Bringing Dynamic Molecular Machines into Focus by Methyl-TROSY NMR

(2014) Rina Rosenzweig et al.   Annual Review of Biochemistry

Cell-free expression of G-protein coupled receptors: new pipelines for challenging targets

(2014) Ralf-Bernhardt Rues et al.   BIOLOGICAL CHEMISTRY

Collective Variable Approaches for Single Molecule Flexible Fitting and Enhanced Sampling

(2014) Harish Vashisth et al.   CHEMICAL REVIEWS

Chemical exchange in biomacromolecules: Past, present, and future

(2014) Arthur G. Palmer   JOURNAL OF MAGNETIC RESONANCE

Visualization of arrestin recruitment by a G-protein-coupled receptor

(2014) Arun K. Shukla et al.   NATURE

Studying “Invisible” Excited Protein States in Slow Exchange with a Major State Conformation

(2012) Pramodh Vallurupalli et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biased Signaling Pathways in  2-Adrenergic Receptor Characterized by 19F-NMR

(2012) J. J. Liu et al.   SCIENCE

Efficacy of the β2-adrenergic receptor is determined by conformational equilibrium in the transmembrane region

(2012) Yutaka Kofuku et al.   Nature Communications

Solution NMR spectroscopy of supra-molecular systems, why bother? A methyl-TROSY view

(2011) Lewis E. Kay   JOURNAL OF MAGNETIC RESONANCE

Current applications of 19F NMR to studies of protein structure and dynamics

(2011) Julianne L. Kitevski-LeBlanc et al.   PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Using relaxation dispersion NMR spectroscopy to determine structures of excited, invisible protein states

(2008) D. Flemming Hansen et al.   JOURNAL OF BIOMOLECULAR NMR

19F NMR applications in chemical biology

(2008) Steven L. Cobb et al.   JOURNAL OF FLUORINE CHEMISTRY

Probing Chemical Shifts of Invisible States of Proteins with Relaxation Dispersion NMR Spectroscopy: How Well Can We Do?

(2008) D. Flemming Hansen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY