The Progress of Intestinal Epithelial Models from Cell Lines to Gut-On-Chip

Genetic engineering in organoids

(2021) Isaree Teriyapirom et al.   JOURNAL OF MOLECULAR MEDICINE-JMM

Gut-on-a-chip: mimicking and monitoring the human intestine

(2021) Denise Marrero et al.   BIOSENSORS & BIOELECTRONICS

Refining Host-Pathogen Interactions: Organ-on-Chip Side of the Coin

(2021) Buket Baddal et al.   Pathogens

Transport of Dietary Anti-Inflammatory Peptide, γ-Glutamyl Valine (γ-EV), across the Intestinal Caco-2 Monolayer

(2021) Snigdha Guha et al.   Nutrients

Organoids and organs-on-chips: Insights into human gut-microbe interactions

(2021) Jens Puschhof et al.   Cell Host & Microbe

Organ-on-chip approaches for intestinal 3D in vitro modelling

(2021) Joana Pimenta et al.   Cellular and Molecular Gastroenterology and Hepatology

Intestinal explant barrier chip: long-term intestinal absorption screening in a novel microphysiological system using tissue explants

(2021) Hossein Eslami Amirabadi et al.   LAB ON A CHIP

Microbiome and Its Role in Irritable Bowel Syndrome

(2020) Mark Pimentel et al.   DIGESTIVE DISEASES AND SCIENCES

Microfluidic chip for culturing intestinal epithelial cell layers: Characterization and comparison of drug transport between dynamic and static models

(2020) Kornphimol Kulthong et al.   TOXICOLOGY IN VITRO

An Interphase Microfluidic Culture System for the Study of Ex Vivo Intestinal Tissue

(2020) Baydoun et al.   Micromachines

Patient-Derived Colorectal Cancer Organoids Upregulate Revival Stem Cell Marker Genes following Chemotherapeutic Treatment

(2020) Engel et al.   Journal of Clinical Medicine

Organoid-based regenerative medicine for inflammatory bowel disease

(2020) Ryuichi Okamoto et al.   Regenerative Therapy

Tools for probing host-bacteria interactions in the gut microenvironment: From molecular to cellular levels

(2020) Kimberly A. Wodzanowski et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

SARS-CoV-2 productively infects human gut enterocytes

(2020) Mart M. Lamers et al.   SCIENCE

Investigating Intestinal Barrier Breakdown in Living Organoids

(2020) Marco Bardenbacher et al.   Jove-Journal of Visualized Experiments

Differential Chloride Secretory Capacity in Transepithelial Ion Transport Properties in Chronic Rhinosinusitis

(2020) Justin McCormick et al.   American Journal of Rhinology & Allergy

Identification of the HT-29 cell line as a model for investigating MCT1 transporters in sigmoid colon adenocarcinoma

(2020) Hashemeya Al-mousawi et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Direct On-Chip Differentiation of Intestinal Tubules from Induced Pluripotent Stem Cells

(2020) Elena Naumovska et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

The Past, Present and Future of Intestinal In Vitro Cell Systems for Drug Absorption Studies

(2020) Sonia Youhanna et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Human organoids: model systems for human biology and medicine

(2020) Jihoon Kim et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Links between Nutrition, Infectious Diseases, and Microbiota: Emerging Technologies and Opportunities for Human-Focused Research

(2020) Manuela Cassotta et al.   Nutrients

A Human 2D Primary Organoid-Derived Epithelial Monolayer Model to Study Host-Pathogen Interaction in the Small Intestine

(2020) Thomas Roodsant et al.   Frontiers in Cellular and Infection Microbiology

An Intestine-on-a-Chip Model of Plug-and-Play Modularity to Study Inflammatory Processes

(2020) Linda Gijzen et al.   SLAS Technology

A Theoretical and Experimental Study to Optimize Cell Differentiation in a Novel Intestinal Chip

(2020) Nicky Langerak et al.   Frontiers in Bioengineering and Biotechnology

Fluid Shear Stress Enhances Differentiation of Jejunal Human Enteroids in Intestine-Chip

(2020) Jianyi Yin et al.   AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY

Gut-on-a-chip: Current progress and future opportunities

(2020) Nureddin Ashammakhi et al.   BIOMATERIALS

Single‐cell RNA sequencing analysis of SARS‐CoV‐2 entry receptors in human organoids

(2020) Rajasekaran Mahalingam et al.   JOURNAL OF CELLULAR PHYSIOLOGY

Gastric organoids—an in vitro model system for the study of gastric development and road to personalized medicine

(2020) Therese Seidlitz et al.   CELL DEATH AND DIFFERENTIATION

Two-Stage Interpretation of Changes in TEER of Intestinal Epithelial Layers Protected by Adhering Bifidobacteria During E. coli Challenges

(2020) Lu Yuan et al.   Frontiers in Microbiology

Evaluating S. flexneri pathogenesis in the human enteroid model

(2019) Sridevi Ranganathan et al.   INFECTION AND IMMUNITY

Human intestinal enteroids as a model system of Shigella pathogenesis

(2019) Benjamin J. Koestler et al.   INFECTION AND IMMUNITY

Species-specific enhancement of enterohemorrhagic E. coli pathogenesis mediated by microbiome metabolites

(2019) Alessio Tovaglieri et al.   Microbiome

Long-term in vitro 3D hydrogel co-culture model of inflammatory bowel disease

(2019) Rasha H. Dosh et al.   Scientific Reports

Using Caffeine and Free Amino Acids To Enhance the Transepithelial Transport of Catechins in Caco-2 Cells

(2019) Yafang Wang et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

A complex human gut microbiome cultured in an anaerobic intestine-on-a-chip

(2019) Sasan Jalili-Firoozinezhad et al.   Nature Biomedical Engineering

Gut Organoids: Mini-Tissues in Culture to Study Intestinal Physiology and Disease

(2019) Mohammad Almeqdadi et al.   AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

The Ussing chamber system for measuring intestinal permeability in health and disease

(2019) Amanda Thomson et al.   BMC GASTROENTEROLOGY

A higher throughput and physiologically relevant two-compartmental human ex vivo intestinal tissue system for studying gastrointestinal processes

(2019) Lianne J. Stevens et al.   EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES

An in vitro intestinal platform with a self-sustaining oxygen gradient to study the human gut/microbiome interface

(2019) Raehyun Kim et al.   Biofabrication

The JAK-Inhibitor Tofacitinib Rescues Human Intestinal Epithelial Cells and Colonoids from Cytokine-Induced Barrier Dysfunction

(2019) Anica Sayoc-Becerra et al.   INFLAMMATORY BOWEL DISEASES

Morphofunctional properties of a differentiated Caco2/HT-29 co-culture as an in vitro model of human intestinal epithelium

(2018) Anita Ferraretto et al.   BIOSCIENCE REPORTS

Reconstruction of the Human Colon Epithelium In Vivo

(2018) Shinya Sugimoto et al.   Cell Stem Cell

Generation of Intestinal Organoids Suitable for Pharmacokinetic Studies from Human Induced Pluripotent Stem Cells

(2018) Daichi Onozato et al.   DRUG METABOLISM AND DISPOSITION

Caco-2 Cell Conditions Enabling Studies of Drug Absorption from Digestible Lipid-Based Formulations

(2018) Janneke Keemink et al.   PHARMACEUTICAL RESEARCH

Intestinal organoids for modelling intestinal development and disease

(2018) Kathryn L. Fair et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip

(2018) Sasan Jalili-Firoozinezhad et al.   Cell Death & Disease

Whole Cell Cross-Linking to Discover Host–Microbe Protein Cognate Receptor/Ligand Pairs

(2018) Bart C. Weimer et al.   Frontiers in Microbiology

Modeling Host-Pathogen Interactions in the Context of the Microenvironment: 3-D Cell Culture Comes of Age

(2018) Jennifer Barrila et al.   INFECTION AND IMMUNITY

Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids

(2018) Magdalena Kasendra et al.   Scientific Reports

Recent progress in organoid culture to model intestinal epithelial barrier functions

(2018) Tetsuya Nakamura   INTERNATIONAL IMMUNOLOGY

GM1-Binding Conjugates To Improve Intestinal Permeability

(2018) Alexandre Melkoumov et al.   MOLECULAR PHARMACEUTICS

Intestinal barrier dysfunction orchestrates the onset of inflammatory host–microbiome cross-talk in a human gut inflammation-on-a-chip

(2018) Woojung Shin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Intestinal in vitro and ex vivo Models to Study Host-Microbiome Interactions and Acute Stressors

(2018) Sarah C. Pearce et al.   Frontiers in Physiology

T84 monolayers are superior to Caco-2 as a model system of colonocytes

(2017) Sarah Devriese et al.   HISTOCHEMISTRY AND CELL BIOLOGY

Factors Affecting the Bioaccessibility and Intestinal Transport of Difenoconazole, Hexaconazole, and Spirodiclofen in Human Caco-2 Cells Following in Vitro Digestion

(2017) Yan-Hong Shi et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Organs-on-Chips with combined multi-electrode array and transepithelial electrical resistance measurement capabilities

(2017) Ben M. Maoz et al.   LAB ON A CHIP

Usefulness of Caco-2/HT29-MTX and Caco-2/HT29-MTX/Raji B Coculture Models To Predict Intestinal and Colonic Permeability Compared to Caco-2 Monoculture

(2017) Isabel Lozoya-Agullo et al.   MOLECULAR PHARMACEUTICS

Development of an in vitro co-culture model to mimic the human intestine in healthy and diseased state

(2017) Angela A.M. Kämpfer et al.   TOXICOLOGY IN VITRO

Disease Modeling in Stem Cell-Derived 3D Organoid Systems

(2017) Devanjali Dutta et al.   TRENDS IN MOLECULAR MEDICINE

Membrane-free culture and real-time barrier integrity assessment of perfused intestinal epithelium tubes

(2017) Sebastiaan J. Trietsch et al.   Nature Communications

A primary human macrophage-enteroid co-culture model to investigate mucosal gut physiology and host-pathogen interactions

(2017) Gaelle Noel et al.   Scientific Reports

Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D

(2017) Arivarasu N. Anabazhagan et al.   Jove-Journal of Visualized Experiments

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

(2017) Marinke W. van der Helm et al.   Jove-Journal of Visualized Experiments

Intestine-on-a-Chip Microfluidic Model for Efficient in Vitro Screening of Oral Chemotherapeutic Uptake

(2017) Kyall Pocock et al.   ACS Biomaterials-Science & Engineering

Intestine-on-a-Chip Microfluidic Model for Efficient in Vitro Screening of Oral Chemotherapeutic Uptake

(2017) Kyall Pocock et al.   ACS Biomaterials Science & Engineering

Human Gut-On-A-Chip Supports Polarized Infection of Coxsackie B1 Virus In Vitro

(2017) Remi Villenave et al.   PLoS One

Characterization of tight junction disruption and immune response modulation in a miniaturized Caco-2/U937 coculture-based in vitro model of the human intestinal barrier

(2016) Qasem Ramadan et al.   BIOMEDICAL MICRODEVICES

A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow

(2016) A. Dawson et al.   Biomicrofluidics

Modeling Development and Disease with Organoids

(2016) Hans Clevers   CELL

Organoids: Modeling Development and the Stem Cell Niche in a Dish

(2016) Kai Kretzschmar et al.   DEVELOPMENTAL CELL

Carotenoid and polyphenol bioaccessibility and cellular uptake from plum and cabbage varieties

(2016) Anouk Kaulmann et al.   FOOD CHEMISTRY

Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology

(2016) Jennifer Foulke-Abel et al.   GASTROENTEROLOGY

Direct 3D-printing of cell-laden constructs in microfluidic architectures

(2016) Justin Liu et al.   LAB ON A CHIP

Organoids as an in vitro model of human development and disease

(2016) Aliya Fatehullah et al.   NATURE CELL BIOLOGY

Human mini-guts: new insights into intestinal physiology and host–pathogen interactions

(2016) Julie G. In et al.   Nature Reviews Gastroenterology & Hepatology

Deterministically patterned biomimetic human iPSC-derived hepatic model via rapid 3D bioprinting

(2016) Xuanyi Ma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

(2016) Hyun Jung Kim et al.   Jove-Journal of Visualized Experiments

“Store-operated” cAMP signaling contributes to Ca2+-activated Cl− secretion in T84 colonic cells

(2015) Jonathan M. Nichols et al.   AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY

Regulation of TWIK-related potassium channel-1 (Trek1) restitutes intestinal epithelial barrier function

(2015) Huang Huang et al.   Cellular & Molecular Immunology

Interaction of Salmonella enterica Serovar Typhimurium with Intestinal Organoids Derived from Human Induced Pluripotent Stem Cells

(2015) Jessica L. Forbester et al.   INFECTION AND IMMUNITY

Measuring direct current trans-epithelial electrical resistance in organ-on-a-chip microsystems

(2015) Mathieu Odijk et al.   LAB ON A CHIP

Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip

(2015) Hyun Jung Kim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Continuous liquid interface production of 3D objects

(2015) J. R. Tumbleston et al.   SCIENCE

Escherichia coli Heat-Stable Enterotoxin Mediates Na+/H+ Exchanger 4 Inhibition Involving cAMP in T84 Human Intestinal Epithelial Cells

(2015) Ana R. Beltrán et al.   PLoS One

A new approach to predict human intestinal absorption using porcine intestinal tissue and biorelevant matrices

(2014) Joost Westerhout et al.   EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES

Human enteroids as an ex-vivo model of host–pathogen interactions in the gastrointestinal tract

(2014) Jennifer Foulke-Abel et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon

(2014) Masayoshi Fukuda et al.   GENES & DEVELOPMENT

3-D Intestinal Scaffolds for Evaluating the Therapeutic Potential of Probiotics

(2014) Cait M. Costello et al.   MOLECULAR PHARMACEUTICS

A small intestinal organoid model of non-invasive enteric pathogen–epithelial cell interactions

(2014) S S Wilson et al.   Mucosal Immunology

Combined probiotic bacteria promotes intestinal epithelial barrier function in interleukin-10-gene-deficient mice

(2014) Chen-Zhang Shi   WORLD JOURNAL OF GASTROENTEROLOGY

Ex vivo culture of intestinal crypt organoids as a model system for assessing cell death induction in intestinal epithelial cells and enteropathy

(2014) T Grabinger et al.   Cell Death & Disease

The application of Ussing chambers for determining the impact of microbes and probiotics on intestinal ion transport

(2013) Kevin W. Lomasney et al.   CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY

Gut-on-a-Chip microenvironment induces human intestinal cells to undergo villus differentiation

(2013) Hyun Jung Kim et al.   Integrative Biology

Improved Transport and Absorption through Gastrointestinal Tract by PEGylated Solid Lipid Nanoparticles

(2013) Hong Yuan et al.   MOLECULAR PHARMACEUTICS

A functional CFTR assay using primary cystic fibrosis intestinal organoids

(2013) Johanna F Dekkers et al.   NATURE MEDICINE

The gut microbiota — masters of host development and physiology

(2013) Felix Sommer et al.   NATURE REVIEWS MICROBIOLOGY

Quantitative Analysis of Molecular Absorption into PDMS Microfluidic Channels

(2012) Jack D. Wang et al.   ANNALS OF BIOMEDICAL ENGINEERING

Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow

(2012) Hyun Jung Kim et al.   LAB ON A CHIP

Characterization of drug permeability in Caco-2 monolayers by mass spectrometry on a membrane-based microfluidic device

(2012) Dan Gao et al.   LAB ON A CHIP

Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5+ stem cell

(2012) Shiro Yui et al.   NATURE MEDICINE

Everted gut sac model as a tool in pharmaceutical research: limitations and applications

(2011) Mohd Aftab Alam et al.   JOURNAL OF PHARMACY AND PHARMACOLOGY

Novel and established intestinal cell line models – An indispensable tool in food science and nutrition

(2011) Tomaz Langerholc et al.   TRENDS IN FOOD SCIENCE & TECHNOLOGY

The Human Gut Microbiome: Ecology and Recent Evolutionary Changes

(2010) Jens Walter et al.   Annual Review of Microbiology

Homeostasis and Inflammation in the Intestine

(2010) Wendy S. Garrett et al.   CELL

Functional cell models of the gut and their applications in food microbiology — A review

(2010) Avrelija Cencič et al.   INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY

A microfluidic approach for in vitro assessment of interorgan interactions in drug metabolism using intestinal and liver slices

(2010) Paul M. van Midwoud et al.   LAB ON A CHIP

Reconstituting Organ-Level Lung Functions on a Chip

(2010) D. Huh et al.   SCIENCE

Intestinal current measurement for diagnostic classification of patients with questionable cystic fibrosis: validation and reference data

(2010) N. Derichs et al.   THORAX

Drug Permeability Assay Using Microhole-Trapped Cells in a Microfluidic Device

(2009) Ju Hun Yeon et al.   ANALYTICAL CHEMISTRY

A Microfluidic System to Evaluate Intestinal Absorption

(2009) Yuki IMURA et al.   ANALYTICAL SCIENCES

Short chain fatty acids exchange across the gut and liver in humans measured at surgery

(2009) Johanne G. Bloemen et al.   CLINICAL NUTRITION

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Lactobacillus rhamnosus GG and Lactobacillus casei Suppress Escherichia coli-Induced Chemokine Expression in Intestinal Epithelial Cells

(2008) Shinji Toki et al.   INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY

An integrated microfluidic system for long-term perfusion culture and on-line monitoring of intestinal tissue models

(2008) Hiroshi Kimura et al.   LAB ON A CHIP