Reconstructing the heart using iPSCs: Engineering strategies and applications

Generation of Vascular Smooth Muscle Cells From Induced Pluripotent Stem Cells

(2021) Mengcheng Shen et al.   CIRCULATION RESEARCH

Generating ring-shaped engineered heart tissues from ventricular and atrial human pluripotent stem cell-derived cardiomyocytes

(2020) Idit Goldfracht et al.   Nature Communications

Effect of Substrate Stiffness on Redox State of Single Cardiomyocyte: A Scanning Electrochemical Microscopy Study

(2020) Yabei Li et al.   ANALYTICAL CHEMISTRY

Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease

(2020) Elisa Giacomelli et al.   Cell Stem Cell

Single-cell RNA sequencing in cardiovascular development, disease and medicine

(2020) David T. Paik et al.   Nature Reviews Cardiology

Designer, injectable gels to prevent transplanted Schwann cell loss during spinal cord injury therapy

(2020) Laura M. Marquardt et al.   Science Advances

Human cardiac organoids for the modelling of myocardial infarction and drug cardiotoxicity

(2020) Dylan J. Richards et al.   Nature Biomedical Engineering

Type V Collagen in Scar Tissue Regulates the Size of Scar after Heart Injury

(2020) Tomohiro Yokota et al.   CELL

Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes

(2020) Jan W. Buikema et al.   Cell Stem Cell

Endogenous Retrovirus-Derived lncRNA BANCR Promotes Cardiomyocyte Migration in Humans and Non-human Primates

(2020) Kitchener D. Wilson et al.   DEVELOPMENTAL CELL

Modeling Secondary Iron Overload Cardiomyopathy with Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

(2020) June-Wha Rhee et al.   Cell Reports

High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays

(2020) Nathalie Brandenberg et al.   Nature Biomedical Engineering

Comparison of 10 Control hPSC Lines for Drug Screening in an Engineered Heart Tissue Format

(2020) Ingra Mannhardt et al.   Stem Cell Reports

Capturing Cardiogenesis in Gastruloids

(2020) Giuliana Rossi et al.   Cell Stem Cell

Patterned human microvascular grafts enable rapid vascularization and increase perfusion in infarcted rat hearts

(2019) Meredith A. Redd et al.   Nature Communications

Mechanical regulation of gene expression in cardiac myocytes and fibroblasts

(2019) Jeffrey J. Saucerman et al.   Nature Reviews Cardiology

Regenerating the field of cardiovascular cell therapy

(2019) Kenneth R. Chien et al.   NATURE BIOTECHNOLOGY

Drug Screening in Human PSC-Cardiac Organoids Identifies Pro-proliferative Compounds Acting via the Mevalonate Pathway

(2019) Richard J. Mills et al.   Cell Stem Cell

A Platform for Generation of Chamber-Specific Cardiac Tissues and Disease Modeling

(2019) Yimu Zhao et al.   CELL

Engineered heart tissue models from hiPSC-derived cardiomyocytes and cardiac ECM for disease modeling and drug testing applications

(2019) Idit Goldfracht et al.   Acta Biomaterialia

Generation of Endothelial Cells From Human Pluripotent Stem Cells

(2019) Ian M. Williams et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Mechanosensing by the Lamina Protects against Nuclear Rupture, DNA Damage, and Cell-Cycle Arrest

(2019) Sangkyun Cho et al.   DEVELOPMENTAL CELL

Generation of Quiescent Cardiac Fibroblasts from Human Induced Pluripotent Stem Cells for In Vitro Modeling of Cardiac Fibrosis

(2019) Hao Zhang et al.   CIRCULATION RESEARCH

3D bioprinting of collagen to rebuild components of the human heart

(2019) A. Lee et al.   SCIENCE

Functional cardiac fibroblasts derived from human pluripotent stem cells via second heart field progenitors

(2019) Jianhua Zhang et al.   Nature Communications

Engineering of human cardiac muscle electromechanically matured to an adult-like phenotype

(2019) Kacey Ronaldson-Bouchard et al.   Nature Protocols

Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels

(2019) Mark A. Skylar-Scott et al.   Science Advances

Effects of Spaceflight on Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Structure and Function

(2019) Alexa Wnorowski et al.   Stem Cell Reports

Progress, obstacles, and limitations in the use of stem cells in organ-on-a-chip models

(2018) Alexa Wnorowski et al.   ADVANCED DRUG DELIVERY REVIEWS

Auxetic Cardiac Patches with Tunable Mechanical and Conductive Properties toward Treating Myocardial Infarction

(2018) Michaella Kapnisi et al.   ADVANCED FUNCTIONAL MATERIALS

Modeling human diseases with induced pluripotent stem cells: from 2D to 3D and beyond

(2018) Chun Liu et al.   DEVELOPMENT

Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions

(2018) Anant Chopra et al.   DEVELOPMENTAL CELL

Advanced maturation of human cardiac tissue grown from pluripotent stem cells

(2018) Kacey Ronaldson-Bouchard et al.   NATURE

Therapeutic approaches for cardiac regeneration and repair

(2018) Hisayuki Hashimoto et al.   Nature Reviews Cardiology

Contractile Work Contributes to Maturation of Energy Metabolism in hiPSC-Derived Cardiomyocytes

(2018) Bärbel M. Ulmer et al.   Stem Cell Reports

Characterization and Validation of a Human 3D Cardiac Microtissue for the Assessment of Changes in Cardiac Pathology

(2018) Caroline R. Archer et al.   Scientific Reports

A tissue-engineered scale model of the heart ventricle

(2018) Luke A. MacQueen et al.   Nature Biomedical Engineering

Optimizing Blended Collagen-Fibrin Hydrogels for Cardiac Tissue Engineering with Human iPSC-derived Cardiomyocytes

(2018) Nicholas J. Kaiser et al.   ACS Biomaterials-Science & Engineering

Mechanosensing by the nucleus: From pathways to scaling relationships

(2017) Sangkyun Cho et al.   JOURNAL OF CELL BIOLOGY

The extracellular matrix in myocardial injury, repair, and remodeling

(2017) Nikolaos G. Frangogiannis   JOURNAL OF CLINICAL INVESTIGATION

Cross-linked matrix rigidity and soluble retinoids synergize in nuclear lamina regulation of stem cell differentiation

(2017) Irena L. Ivanovska et al.   MOLECULAR BIOLOGY OF THE CELL

Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions

(2017) Xiaoping Bao et al.   Nature Protocols

Mechanosensing of matrix by stem cells: From matrix heterogeneity, contractility, and the nucleus in pore-migration to cardiogenesis and muscle stem cells in vivo

(2017) Lucas Smith et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

Passive Stretch Induces Structural and Functional Maturation of Engineered Heart Muscle as Predicted by Computational Modeling

(2017) Oscar J. Abilez et al.   STEM CELLS

Region and cell-type resolved quantitative proteomic map of the human heart

(2017) Sophia Doll et al.   Nature Communications

Combinatorial Extracellular Matrix Microenvironments for Probing Endothelial Differentiation of Human Pluripotent Stem Cells

(2017) Luqia Hou et al.   Scientific Reports

Efficient Large-Scale 2D Culture System for Human Induced Pluripotent Stem Cells and Differentiated Cardiomyocytes

(2017) Shugo Tohyama et al.   Stem Cell Reports

Nanotopography-Induced Structural Anisotropy and Sarcomere Development in Human Cardiomyocytes Derived from Induced Pluripotent Stem Cells

(2016) Daniel Carson et al.   ACS Applied Materials & Interfaces

Mechanical stimulation in the engineering of heart muscle

(2016) Norman Yu Liaw et al.   ADVANCED DRUG DELIVERY REVIEWS

Dynamic culture yields engineered myocardium with near-adult functional output

(2016) Christopher P. Jackman et al.   BIOMATERIALS

Mechanical Stress Conditioning and Electrical Stimulation Promote Contractility and Force Maturation of Induced Pluripotent Stem Cell-Derived Human Cardiac TissueClinical Perspective

(2016) Jia-Ling Ruan et al.   CIRCULATION

Recounting Cardiac Cellular Composition

(2016) Pingzhu Zhou et al.   CIRCULATION RESEARCH

Mechanical Regulation of Cardiac Aging in Model Systems

(2016) Ayla O. Sessions et al.   CIRCULATION RESEARCH

Biodegradable scaffold with built-in vasculature for organ-on-a-chip engineering and direct surgical anastomosis

(2016) Boyang Zhang et al.   NATURE MATERIALS

Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing

(2016) Johan U. Lind et al.   NATURE MATERIALS

Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells

(2016) Nathan J Palpant et al.   Nature Protocols

Induced pluripotent stem cells: at the heart of cardiovascular precision medicine

(2016) Ian Y. Chen et al.   Nature Reviews Cardiology

Mechanical signaling coordinates the embryonic heartbeat

(2016) Kevin K. Chiou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Distilling complexity to advance cardiac tissue engineering

(2016) Brenda M. Ogle et al.   Science Translational Medicine

Cardiac repair in guinea pigs with human engineered heart tissue from induced pluripotent stem cells

(2016) F. Weinberger et al.   Science Translational Medicine

Stromal Cells in Dense Collagen Promote Cardiomyocyte and Microvascular Patterning in Engineered Human Heart Tissue

(2016) Meredith A. Roberts et al.   TISSUE ENGINEERING PART A

Real-Time Force and Frequency Analysis of Engineered Human Heart Tissue Derived from Induced Pluripotent Stem Cells Using Magnetic Sensing

(2016) Kevin S. Bielawski et al.   TISSUE ENGINEERING PART C-METHODS

Integrative Analysis of PRKAG2 Cardiomyopathy iPS and Microtissue Models Identifies AMPK as a Regulator of Metabolism, Survival, and Fibrosis

(2016) J. Travis Hinson et al.   Cell Reports

Mechanotransduction and Metabolism in Cardiomyocyte Microdomains

(2016) Francesco S. Pasqualini et al.   Biomed Research International

A conducting polymer with enhanced electronic stability applied in cardiac models

(2016) D. Mawad et al.   Science Advances

Miniaturized iPS-Cell-Derived Cardiac Muscles for Physiologically Relevant Drug Response Analyses

(2016) Nathaniel Huebsch et al.   Scientific Reports

Injectable biodegradable hybrid hydrogels based on thiolated collagen and oligo(acryloyl carbonate)–poly(ethylene glycol)–oligo(acryloyl carbonate) copolymer for functional cardiac regeneration

(2015) Guohui Xu et al.   Acta Biomaterialia

Physiologic force-frequency response in engineered heart muscle by electromechanical stimulation

(2015) Amandine F.G. Godier-Furnémont et al.   BIOMATERIALS

Dynamics of Cell Generation and Turnover in the Human Heart

(2015) Olaf Bergmann et al.   CELL

A Conductive Polymer Hydrogel Supports Cell Electrical Signaling and Improves Cardiac Function After Implantation into Myocardial Infarct

(2015) Anton Mihic et al.   CIRCULATION

Human Engineered Heart Muscles Engraft and Survive Long Term in a Rodent Myocardial Infarction ModelNovelty and Significance

(2015) Johannes Riegler et al.   CIRCULATION RESEARCH

Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells

(2015) Christoph Patsch et al.   NATURE CELL BIOLOGY

Contractility of single cardiomyocytes differentiated from pluripotent stem cells depends on physiological shape and substrate stiffness

(2015) Alexandre J. S. Ribeiro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy

(2015) J. T. Hinson et al.   SCIENCE

Development of a scalable suspension culture for cardiac differentiation from human pluripotent stem cells

(2015) Vincent C. Chen et al.   Stem Cell Research

Self-organizing human cardiac microchambers mediated by geometric confinement

(2015) Zhen Ma et al.   Nature Communications

An integrated statistical model for enhanced murine cardiomyocyte differentiation via optimized engagement of 3D extracellular matrices

(2015) Jangwook P. Jung et al.   Scientific Reports

Reduced matrix rigidity promotes neonatal cardiomyocyte dedifferentiation, proliferation and clonal expansion

(2015) Yfat Yahalom-Ronen et al.   eLife

Heart Regeneration with Engineered Myocardial Tissue

(2014) Kareen L.K. Coulombe et al.   Annual Review of Biomedical Engineering

Effects of proarrhythmic drugs on relaxation time and beating pattern in rat engineered heart tissue

(2014) Alexandra Eder et al.   BASIC RESEARCH IN CARDIOLOGY

Hybrid Elastin-like Polypeptide–Polyethylene Glycol (ELP-PEG) Hydrogels with Improved Transparency and Independent Control of Matrix Mechanics and Cell Ligand Density

(2014) Huiyuan Wang et al.   BIOMACROMOLECULES

Cardiac Tissue Slice Transplantation as a Model to Assess Tissue-Engineered Graft Thickness, Survival, and Function

(2014) J. Riegler et al.   CIRCULATION

Functional improvement and maturation of rat and human engineered heart tissue by chronic electrical stimulation

(2014) Marc N. Hirt et al.   JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY

Generation of the epicardial lineage from human pluripotent stem cells

(2014) Alec D Witty et al.   NATURE BIOTECHNOLOGY

Modeling the mitochondrial cardiomyopathy of Barth syndrome with induced pluripotent stem cell and heart-on-chip technologies

(2014) Gang Wang et al.   NATURE MEDICINE

Chemically defined generation of human cardiomyocytes

(2014) Paul W Burridge et al.   NATURE METHODS

PlGF–MMP9-engineered iPS cells supported on a PEG–fibrinogen hydrogel scaffold possess an enhanced capacity to repair damaged myocardium

(2014) C Bearzi et al.   Cell Death & Disease

Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink

(2014) Falguni Pati et al.   Nature Communications

Efficient Differentiation of Human Pluripotent Stem Cells to Endothelial Progenitors via Small-Molecule Activation of WNT Signaling

(2014) Xiaojun Lian et al.   Stem Cell Reports

Functionality of Endothelial Cells and Pericytes From Human Pluripotent Stem Cells Demonstrated in Cultured Vascular Plexus and Zebrafish Xenografts

(2013) Valeria V. Orlova et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Tissue-engineered cardiac patch for advanced functional maturation of human ESC-derived cardiomyocytes

(2013) Donghui Zhang et al.   BIOMATERIALS

Three-dimensional filamentous human diseased cardiac tissue model

(2013) Zhen Ma et al.   BIOMATERIALS

Forces in Tissue Morphogenesis and Patterning

(2013) Carl-Philipp Heisenberg et al.   CELL

Heart-Specific Stiffening in Early Embryos Parallels Matrix and Myosin Expression to Optimize Beating

(2013) Stephanie Majkut et al.   CURRENT BIOLOGY

Microfabricated perfusable cardiac biowire: a platform that mimics native cardiac bundle

(2013) Yun Xiao et al.   LAB ON A CHIP

Biowire: a platform for maturation of human pluripotent stem cell–derived cardiomyocytes

(2013) Sara S Nunes et al.   NATURE METHODS

Design and formulation of functional pluripotent stem cell-derived cardiac microtissues

(2013) N. Thavandiran et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In Vitro Engineering of Vascularized Tissue Surrogates

(2013) Katsuhisa Sakaguchi et al.   Scientific Reports

Cellular Encapsulation Enhances Cardiac Repair

(2013) Rebecca D. Levit et al.   Journal of the American Heart Association

Production of De Novo Cardiomyocytes: Human Pluripotent Stem Cell Differentiation and Direct Reprogramming

(2012) Paul W. Burridge et al.   Cell Stem Cell

Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells to Cardiomyocytes

(2012) Christine L. Mummery et al.   CIRCULATION RESEARCH

Murine and human pluripotent stem cell-derived cardiac bodies form contractile myocardial tissue in vitro

(2012) George Kensah et al.   EUROPEAN HEART JOURNAL

Generation of human vascular smooth muscle subtypes provides insight into embryological origin–dependent disease susceptibility

(2012) Christine Cheung et al.   NATURE BIOTECHNOLOGY

Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions

(2012) Xiaojun Lian et al.   Nature Protocols

Cozzarelli Prize Winner: Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling

(2012) X. Lian et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Hydrogel crosslinking density regulates temporal contractility of human embryonic stem cell-derived cardiomyocytes in 3D cultures

(2012) Cindy Chung et al.   Soft Matter

Cardiac tissue engineering using tissue printing technology and human cardiac progenitor cells

(2011) Roberto Gaetani et al.   BIOMATERIALS

Neonatal Mouse–Derived Engineered Cardiac Tissue

(2011) W.J. de Lange et al.   CIRCULATION RESEARCH

Terminal Differentiation, Advanced Organotypic Maturation, and Modeling of Hypertrophic Growth in Engineered Heart Tissue

(2011) Malte Tiburcy et al.   CIRCULATION RESEARCH

Human Engineered Heart Tissue as a Versatile Tool in Basic Research and Preclinical Toxicology

(2011) Sebastian Schaaf et al.   PLoS One

A Microfabricated Platform to Measure and Manipulate the Mechanics of Engineered Cardiac Microtissues

(2011) Thomas Boudou et al.   TISSUE ENGINEERING PART A

Development of a Drug Screening Platform Based on Engineered Heart Tissue

(2010) Arne Hansen et al.   CIRCULATION RESEARCH

Cardiac Development and Implications for Heart Disease

(2010) Jonathan A. Epstein   NEW ENGLAND JOURNAL OF MEDICINE

Mesoscopic hydrogel molding to control the 3D geometry of bioartificial muscle tissues

(2009) Weining Bian et al.   Nature Protocols

Electrical stimulation systems for cardiac tissue engineering

(2009) Nina Tandon et al.   Nature Protocols

Nanoscale cues regulate the structure and function of macroscopic cardiac tissue constructs

(2009) D.-H. Kim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Substrate Stiffness Affects the Functional Maturation of Neonatal Rat Ventricular Myocytes

(2008) Jeffrey G. Jacot et al.   BIOPHYSICAL JOURNAL

Effect of Injectable Alginate Implant on Cardiac Remodeling and Function After Recent and Old Infarcts in Rat

(2008) Natali Landa et al.   CIRCULATION

Embryonic cardiomyocytes beat best on a matrix with heart-like elasticity: scar-like rigidity inhibits beating

(2008) A. J. Engler et al.   JOURNAL OF CELL SCIENCE

Accordion-like honeycombs for tissue engineering of cardiac anisotropy

(2008) George C. Engelmayr et al.   NATURE MATERIALS

Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart

(2008) Harald C Ott et al.   NATURE MEDICINE