TopBP1 assembles nuclear condensates to switch on ATR signaling

Mouse Heterochromatin Adopts Digital Compaction States without Showing Hallmarks of HP1-Driven Liquid-Liquid Phase Separation

(2020) Fabian Erdel et al.   MOLECULAR CELL

Protein Phase Separation as a Stress Survival Strategy

(2019) Titus M. Franzmann et al.   Cold Spring Harbor Perspectives in Biology

MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis

(2019) Pia-Amata Leimbacher et al.   MOLECULAR CELL

Quantitative phosphoproteomics reveals mitotic function of the ATR activator ETAA1

(2019) Thomas E. Bass et al.   JOURNAL OF CELL BIOLOGY

Common motifs in ETAA1 and TOPBP1 required for ATR kinase activation

(2019) Vaughn Thada et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Evidence for DNA-mediated nuclear compartmentalization distinct from phase separation

(2019) David Trombley McSwiggen et al.   eLife

Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology

(2019) Peipei Zhang et al.   eLife

Phase separation of 53 BP 1 determines liquid‐like behavior of DNA repair compartments

(2019) Sinan Kilic et al.   EMBO JOURNAL

Basal CHK1 activity safeguards its stability to maintain intrinsic S-phase checkpoint functions

(2019) Jone Michelena et al.   JOURNAL OF CELL BIOLOGY

Functional transcription promoters at DNA double-strand breaks mediate RNA-driven phase separation of damage-response factors

(2019) Fabio Pessina et al.   NATURE CELL BIOLOGY

Probing and engineering liquid-phase organelles

(2019) Dan Bracha et al.   NATURE BIOTECHNOLOGY

Mechanisms for Active Regulation of Biomolecular Condensates

(2019) Johannes Söding et al.   TRENDS IN CELL BIOLOGY

A User’s Guide for Phase Separation Assays with Purified Proteins

(2018) Simon Alberti et al.   JOURNAL OF MOLECULAR BIOLOGY

DNA-induced liquid phase condensation of cGAS activates innate immune signaling

(2018) Mingjian Du et al.   SCIENCE

Coactivator condensation at super-enhancers links phase separation and gene control

(2018) Benjamin R. Sabari et al.   SCIENCE

Phase separation of a yeast prion protein promotes cellular fitness

(2018) Titus M. Franzmann et al.   SCIENCE

Phase-separation mechanism for C-terminal hyperphosphorylation of RNA polymerase II

(2018) Huasong Lu et al.   NATURE

Efficient proximity labeling in living cells and organisms with TurboID

(2018) Tess C Branon et al.   NATURE BIOTECHNOLOGY

Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains

(2018) Ann Boija et al.   CELL

Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome

(2018) Yongdae Shin et al.   CELL

Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds

(2018) Dan Bracha et al.   CELL

TOPBP1Dpb11plays a conserved role in homologous recombination DNA repair through the coordinated recruitment of 53BP1Rad9

(2017) Yi Liu et al.   JOURNAL OF CELL BIOLOGY

The essential kinase ATR: ensuring faithful duplication of a challenging genome

(2017) Joshua C. Saldivar et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

3.9 Å structure of the yeast Mec1-Ddc2 complex, a homolog of human ATR-ATRIP

(2017) Xuejuan Wang et al.   SCIENCE

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Proteomics Reveals Global Regulation of Protein SUMOylation by ATM and ATR Kinases during Replication Stress

(2017) Stephanie Munk et al.   Cell Reports

RPA-Binding Protein ETAA1 Is an ATR Activator Involved in DNA Replication Stress Response

(2016) Yuan-Cho Lee et al.   CURRENT BIOLOGY

Direct Binding to Replication Protein A (RPA)-coated Single-stranded DNA Allows Recruitment of the ATR Activator TopBP1 to Sites of DNA Damage

(2016) Julyana Acevedo et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity

(2016) Pavel Moudry et al.   JOURNAL OF CELL BIOLOGY

ETAA1 acts at stalled replication forks to maintain genome integrity

(2016) Thomas E. Bass et al.   NATURE CELL BIOLOGY

Activation of the ATR kinase by the RPA-binding protein ETAA1

(2016) Peter Haahr et al.   NATURE CELL BIOLOGY

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

(2015) Avinash Patel et al.   CELL

TopBP1 is required at mitosis to reduce transmission of DNA damage to G1 daughter cells

(2015) Rune Troelsgaard Pedersen et al.   JOURNAL OF CELL BIOLOGY

High levels of TopBP1 induce ATR-dependent shut-down of rRNA transcription and nucleolar segregation

(2015) Miiko Sokka et al.   NUCLEIC ACIDS RESEARCH

Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose)

(2015) Matthias Altmeyer et al.   Nature Communications

TOPBP1 recruits TOP2A to ultra-fine anaphase bridges to aid in their resolution

(2015) Ronan Broderick et al.   Nature Communications

Targeting TopBP1 at a convergent point of multiple oncogenic pathways for cancer therapy

(2014) Pinki Chowdhury et al.   Nature Communications

Phosphorylation-Regulated Binding of RNA Polymerase II to Fibrous Polymers of Low-Complexity Domains

(2013) Ilmin Kwon et al.   CELL

DNA structure-specific priming of ATR activation by DNA-PKcs

(2013) Sophie Vidal-Eychenié et al.   JOURNAL OF CELL BIOLOGY

A Role for the MRN Complex in ATR Activation via TOPBP1 Recruitment

(2013) Anja M. Duursma et al.   MOLECULAR CELL

Optogenetic protein clustering and signaling activation in mammalian cells

(2013) Lukasz J Bugaj et al.   NATURE METHODS

DNA Damage Sensing by the ATM and ATR Kinases

(2013) A. Marechal et al.   Cold Spring Harbor Perspectives in Biology

An Essential Function for the ATR-Activation-Domain (AAD) of TopBP1 in Mouse Development and Cellular Senescence

(2013) Zhong-Wei Zhou et al.   PLoS Genetics

Beyond Oil and Water--Phase Transitions in Cells

(2012) A. A. Hyman et al.   SCIENCE

The Rad4TopBP1 ATR-Activation Domain Functions in G1/S Phase in a Chromatin-Dependent Manner

(2012) Su-Jiun Lin et al.   PLoS Genetics

TopBP1 and DNA polymerase alpha-mediated recruitment of the 9-1-1 complex to stalled replication forks: Implications for a replication restart-based mechanism for ATR checkpoint activation

(2011) Shan Yan et al.   CELL CYCLE

Tethering DNA Damage Checkpoint Mediator Proteins Topoisomerase IIβ-binding Protein 1 (TopBP1) and Claspin to DNA Activates Ataxia-Telangiectasia Mutated and RAD3-related (ATR) Phosphorylation of Checkpoint Kinase 1 (Chk1)

(2011) Laura A. Lindsey-Boltz et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Discovery of Potent and Selective Inhibitors of Ataxia Telangiectasia Mutated and Rad3 Related (ATR) Protein Kinase as Potential Anticancer Agents

(2011) Jean-Damien Charrier et al.   JOURNAL OF MEDICINAL CHEMISTRY

ATR Autophosphorylation as a Molecular Switch for Checkpoint Activation

(2011) Shizhou Liu et al.   MOLECULAR CELL

A cell-based screen identifies ATR inhibitors with synthetic lethal properties for cancer-associated mutations

(2011) Luis I Toledo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

14-3-3γ mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage

(2010) Kousuke Kasahara et al.   EMBO JOURNAL

TopBP1 functions with 53BP1 in the G1 DNA damage checkpoint

(2010) Rachele Cescutti et al.   EMBO JOURNAL

Continued primer synthesis at stalled replication forks contributes to checkpoint activation

(2010) Christopher Van et al.   JOURNAL OF CELL BIOLOGY

The DNA Damage Response: Making It Safe to Play with Knives

(2010) Alberto Ciccia et al.   MOLECULAR CELL

Direct visualization of the co-transcriptional assembly of a nuclear body by noncoding RNAs

(2010) Yuntao S. Mao et al.   NATURE CELL BIOLOGY

Regulation of p53 by TopBP1: a Potential Mechanism for p53 Inactivation in Cancer

(2009) K. Liu et al.   MOLECULAR AND CELLULAR BIOLOGY

The Mre11-Rad50-Nbs1 Complex Mediates Activation of TopBP1 by ATM

(2009) Hae Yong Yoo et al.   MOLECULAR BIOLOGY OF THE CELL

The DNA-damage response in human biology and disease

(2009) Stephen P. Jackson et al.   NATURE

TopBP1 activates ATR through ATRIP and a PIKK regulatory domain

(2008) D. A. Mordes et al.   GENES & DEVELOPMENT

How ATR turns on: TopBP1 goes on ATRIP with ATR

(2008) A. E. Burrows et al.   GENES & DEVELOPMENT

Colocalization of Sensors Is Sufficient to Activate the DNA Damage Checkpoint in the Absence of Damage

(2008) Carla Yaneth Bonilla et al.   MOLECULAR CELL

MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification

(2008) Jürgen Cox et al.   NATURE BIOTECHNOLOGY