Liquid phase condensation in cell physiology and disease

RNA phase transitions in repeat expansion disorders

(2017) Ankur Jain et al.   NATURE

Liquid droplet formation by HP1α suggests a role for phase separation in heterochromatin

(2017) Adam G. Larson et al.   NATURE

Phase behaviour of disordered proteins underlying low density and high permeability of liquid organelles

(2017) Ming-Tzo Wei et al.   Nature Chemistry

Programming molecular self-assembly of intrinsically disordered proteins containing sequences of low complexity

(2017) Joseph R. Simon et al.   Nature Chemistry

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Liquid behavior of cross-linked actin bundles

(2017) Kimberly L. Weirich et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Independent active and thermodynamic processes govern the nucleolus assembly in vivo

(2017) Hanieh Falahati et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Direct observation of structure and dynamics during phase separation of an elastomeric protein

(2017) Sean E. Reichheld et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ATP as a biological hydrotrope

(2017) Avinash Patel et al.   SCIENCE

The synaptonemal complex has liquid crystalline properties and spatially regulates meiotic recombination factors

(2017) Ofer Rog et al.   eLife

Amyloid-like Self-Assembly of a Cellular Compartment

(2016) Elvan Boke et al.   CELL

Phase Transition in Postsynaptic Densities Underlies Formation of Synaptic Complexes and Synaptic Plasticity

(2016) Menglong Zeng et al.   CELL

Enhancer Control of Transcriptional Bursting

(2016) Takashi Fukaya et al.   CELL

Polar Positioning of Phase-Separated Liquid Compartments in Cells Regulated by an mRNA Competition Mechanism

(2016) Shambaditya Saha et al.   CELL

ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure

(2016) Saumya Jain et al.   CELL

Compositional Control of Phase-Separated Cellular Bodies

(2016) Salman F. Banani et al.   CELL

Coexisting Liquid Phases Underlie Nucleolar Subcompartments

(2016) Marina Feric et al.   CELL

Nucleation by rRNA Dictates the Precision of Nucleolus Assembly

(2016) Hanieh Falahati et al.   CURRENT BIOLOGY

A glass menagerie of low complexity sequences

(2016) Randal Halfmann   CURRENT OPINION IN STRUCTURAL BIOLOGY

Structural, super-resolution microscopy analysis of paraspeckle nuclear body organization

(2016) Jason A. West et al.   JOURNAL OF CELL BIOLOGY

A Surveillance Function of the HSPB8-BAG3-HSP70 Chaperone Complex Ensures Stress Granule Integrity and Dynamism

(2016) Massimo Ganassi et al.   MOLECULAR CELL

Sequence Determinants of Intracellular Phase Separation by Complex Coacervation of a Disordered Protein

(2016) Chi W. Pak et al.   MOLECULAR CELL

Ki-67 acts as a biological surfactant to disperse mitotic chromosomes

(2016) Sara Cuylen et al.   NATURE

Membraneless organelles can melt nucleic acid duplexes and act as biomolecular filters

(2016) Timothy J. Nott et al.   Nature Chemistry

Growth and division of active droplets provides a model for protocells

(2016) David Zwicker et al.   Nature Physics

Long non-coding RNAs: spatial amplifiers that control nuclear structure and gene expression

(2016) Jesse M. Engreitz et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Sequence-Specific Polyampholyte Phase Separation in Membraneless Organelles

(2016) Yi-Hsuan Lin et al.   PHYSICAL REVIEW LETTERS

In vivo aspects of protein folding and quality control

(2016) D. Balchin et al.   SCIENCE

Phase separation of signaling molecules promotes T cell receptor signal transduction

(2016) Xiaolei Su et al.   SCIENCE

ALS Mutations Disrupt Phase Separation Mediated by α-Helical Structure in the TDP-43 Low-Complexity C-Terminal Domain

(2016) Alexander E. Conicella et al.   STRUCTURE

Transport Selectivity of Nuclear Pores, Phase Separation, and Membraneless Organelles

(2016) H. Broder Schmidt et al.   TRENDS IN BIOCHEMICAL SCIENCES

Principles and Properties of Stress Granules

(2016) David S.W. Protter et al.   TRENDS IN CELL BIOLOGY

Cajal bodies are linked to genome conformation

(2016) Qiuyan Wang et al.   Nature Communications

In Vivo Formation of Vacuolated Multi-phase Compartments Lacking Membranes

(2016) Hermann Broder Schmidt et al.   Cell Reports

Real-time imaging of Huntingtin aggregates diverting target search and gene transcription

(2016) Li Li et al.   eLife

A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy

(2016) Matthias Christoph Munder et al.   eLife

ATPase activity of the DEAD-box protein Dhh1 controls processing body formation

(2016) Christopher Frederick Mugler et al.   eLife

CPEB4 is regulated during cell cycle by ERK2/Cdk1-mediated phosphorylation and its assembly into liquid-like droplets

(2016) Jordina Guillén-Boixet et al.   eLife

Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

(2016) Jarrett Smith et al.   eLife

Nucleophosmin integrates within the nucleolus via multi-modal interactions with proteins displaying R-rich linear motifs and rRNA

(2016) Diana M Mitrea et al.   eLife

Distinct stages in stress granule assembly and disassembly

(2016) Joshua R Wheeler et al.   eLife

The LC Domain of hnRNPA2 Adopts Similar Conformations in Hydrogel Polymers, Liquid-like Droplets, and Nuclei

(2015) Siheng Xiang et al.   CELL

Phase Separation by Low Complexity Domains Promotes Stress Granule Assembly and Drives Pathological Fibrillization

(2015) Amandine Molliex et al.   CELL

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

(2015) Avinash Patel et al.   CELL

Phase Transition of Spindle-Associated Protein Regulate Spindle Apparatus Assembly

(2015) Hao Jiang et al.   CELL

Inverse Size Scaling of the Nucleolus by a Concentration-Dependent Phase Transition

(2015) Stephanie C. Weber et al.   CURRENT BIOLOGY

Relating sequence encoded information to form and function of intrinsically disordered proteins

(2015) Rahul K Das et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

RNA Controls PolyQ Protein Phase Transitions

(2015) Huaiying Zhang et al.   MOLECULAR CELL

Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins

(2015) Yuan Lin et al.   MOLECULAR CELL

Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II

(2015) Kathleen A. Burke et al.   MOLECULAR CELL

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

Dynamically reconfigurable complex emulsions via tunable interfacial tensions

(2015) Lauren D. Zarzar et al.   NATURE

Phosphorylation-mediated RNA/peptide complex coacervation as a model for intracellular liquid organelles

(2015) William M. Aumiller et al.   Nature Chemistry

Polycomb repressive complex PRC1 spatially constrains the mouse embryonic stem cell genome

(2015) Stefan Schoenfelder et al.   NATURE GENETICS

Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers

(2015) Felipe García Quiroz et al.   NATURE MATERIALS

Polymer physics of intracellular phase transitions

(2015) Clifford P. Brangwynne et al.   Nature Physics

Three-dimensional genome architecture: players and mechanisms

(2015) Ana Pombo et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

ALS/FTD Mutation-Induced Phase Transition of FUS Liquid Droplets and Reversible Hydrogels into Irreversible Hydrogels Impairs RNP Granule Function

(2015) Tetsuro Murakami et al.   NEURON

RNA transcription modulates phase transition-driven nuclear body assembly

(2015) Joel Berry et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics

(2015) Shana Elbaum-Garfinkle et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose)

(2015) Matthias Altmeyer et al.   Nature Communications

Promiscuous interactions and protein disaggregases determine the material state of stress-inducible RNP granules

(2015) Sonja Kroschwald et al.   eLife

Assemblages: Functional units formed by cellular phase separation

(2014) Jeffrey A. Toretsky et al.   JOURNAL OF CELL BIOLOGY

Centrosomes are autocatalytic droplets of pericentriolar material organized by centrioles

(2014) David Zwicker et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Physical basis of spindle self-organization

(2014) Jan Brugués et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans

(2014) Jennifer T Wang et al.   eLife

Phase transitions of multivalent proteins can promote clustering of membrane receptors

(2014) Sudeep Banjade et al.   eLife

Eukaryotic Stress Granules Are Cleared by Autophagy and Cdc48/VCP Function

(2013) J. Ross Buchan et al.   CELL

Phosphorylation-Regulated Binding of RNA Polymerase II to Fibrous Polymers of Low-Complexity Domains

(2013) Ilmin Kwon et al.   CELL

Dual Specificity Kinase DYRK3 Couples Stress Granule Condensation/Dissolution to mTORC1 Signaling

(2013) Frank Wippich et al.   CELL

The Bacterial Cytoplasm Has Glass-like Properties and Is Fluidized by Metabolic Activity

(2013) Bradley R. Parry et al.   CELL

Coordination of Translational Control and Protein Homeostasis during Severe Heat Stress

(2013) Valeria Cherkasov et al.   CURRENT BIOLOGY

Translation Repressors, an RNA Helicase, and Developmental Cues Control RNP Phase Transitions during Early Development

(2013) Arnaud Hubstenberger et al.   DEVELOPMENTAL CELL

XMAP215 activity sets spindle length by controlling the total mass of spindle microtubules

(2013) Simone B. Reber et al.   NATURE CELL BIOLOGY

A nuclear F-actin scaffold stabilizes ribonucleoprotein droplets against gravity in large cells

(2013) Marina Feric et al.   NATURE CELL BIOLOGY

Spatial Organization of the Cell Cytoplasm by Position-Dependent Phase Separation

(2013) Chiu Fan Lee et al.   PHYSICAL REVIEW LETTERS

Conformations of intrinsically disordered proteins are influenced by linear sequence distributions of oppositely charged residues

(2013) R. K. Das et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell-free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers within Hydrogels

(2012) Masato Kato et al.   CELL

Getting RNA and Protein in Phase

(2012) Stephanie C. Weber et al.   CELL

Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins

(2012) Alfredo Castello et al.   CELL

Metazoan Hsp70 machines use Hsp110 to power protein disaggregation

(2012) Heike Rampelt et al.   EMBO JOURNAL

Mixing Liquids—Mission Impossible? A Colorful Demonstration on Immiscible Systems

(2012) Jens Eckelmann et al.   JOURNAL OF CHEMICAL EDUCATION

Transient Sequestration of TORC1 into Stress Granules during Heat Stress

(2012) Terunao Takahara et al.   MOLECULAR CELL

In Retrospect: The Origin of Life

(2012) Tony Hyman et al.   NATURE

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

RNA catalysis through compartmentalization

(2012) Christopher A. Strulson et al.   Nature Chemistry

Intrinsically disordered proteins: a 10-year recap

(2012) Peter Tompa   TRENDS IN BIOCHEMICAL SCIENCES

Regulation of the MEX-5 Gradient by a Spatially Segregated Kinase/Phosphatase Cycle

(2011) Erik E. Griffin et al.   CELL

Signaling clusters in the cell membrane

(2011) Niña C Hartman et al.   CURRENT OPINION IN CELL BIOLOGY

Nucleation of nuclear bodies by RNA

(2011) Sergey P. Shevtsov et al.   NATURE CELL BIOLOGY

Peptide–nucleotide microdroplets as a step towards a membrane-free protocell model

(2011) Shogo Koga et al.   Nature Chemistry

The Mammalian Disaggregase Machinery: Hsp110 Synergizes with Hsp70 and Hsp40 to Catalyze Protein Disaggregation and Reactivation in a Cell-Free System

(2011) James Shorter   PLoS One

Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes

(2011) C. P. Brangwynne et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome organization in and around the nucleolus

(2011) Attila Németh et al.   TRENDS IN GENETICS

Biogenesis and function of nuclear bodies

(2011) Yuntao S. Mao et al.   TRENDS IN GENETICS

An inducible nuclear body in theDrosophilagerminal vesicle

(2011) Alison B. Singer et al.   Nucleus

Perinuclear P granules are the principal sites of mRNA export in adult C. elegans germ cells

(2010) U. Sheth et al.   DEVELOPMENT

P Granule Assembly and Function in Caenorhabditis elegans Germ Cells

(2010) D. Updike et al.   JOURNAL OF ANDROLOGY

Three-dimensional organization of promyelocytic leukemia nuclear bodies

(2010) M. Lang et al.   JOURNAL OF CELL SCIENCE

Direct visualization of the co-transcriptional assembly of a nuclear body by noncoding RNAs

(2010) Yuntao S. Mao et al.   NATURE CELL BIOLOGY

Reaction-Diffusion Model as a Framework for Understanding Biological Pattern Formation

(2010) S. Kondo et al.   SCIENCE

Phase transitions of folded proteins

(2010) Peter G. Vekilov   Soft Matter

The Nucleolus

(2010) T. Pederson   Cold Spring Harbor Perspectives in Biology

Cytoplasmic ribonucleoprotein (RNP) bodies and their relationship to GW/P bodies

(2009) Joanna J. Moser et al.   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

Cajal-body formation correlates with differential coilin phosphorylation in primary and transformed cell lines

(2009) S. M. Hearst et al.   JOURNAL OF CELL SCIENCE

RNA granules: post-transcriptional and epigenetic modulators of gene expression

(2009) Paul Anderson et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

Gelation of particles with short-range attraction

(2008) Peter J. Lu et al.   NATURE

Spatial Regulators for Bacterial Cell Division Self-Organize into Surface Waves in Vitro

(2008) M. Loose et al.   SCIENCE