Imaging Platelet Processes and Function—Current and Emerging Approaches for Imaging in vitro and in vivo

Use of electron microscopy to study megakaryocytes

(2020) Cyril Scandola et al.   PLATELETS

Contractile forces in platelet aggregates under microfluidic shear gradients reflect platelet inhibition and bleeding risk

(2019) Lucas H. Ting et al.   Nature Communications

Autologous platelet-rich plasma for healing chronic venous leg ulcers: Clinical efficacy and potential mechanisms

(2019) Carolina D. Weller et al.   International Wound Journal

Interrelationships between structure and function during the hemostatic response to injury

(2019) Maurizio Tomaiuolo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Electrostatic Steering Enables Flow-Activated Von Willebrand Factor to Bind Platelet Glycoprotein, Revealed by Single-Molecule Stretching and Imaging

(2019) Yan Jiang et al.   JOURNAL OF MOLECULAR BIOLOGY

Image deconvolution for confocal laser scanning microscopy using constrained total variation with a gradient field

(2019) Tao He et al.   APPLIED OPTICS

Targeting Glycoprotein VI for Thromboembolic Disorders

(2019) Frederik Denorme et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Safety and Tolerability, Pharmacokinetics, and Pharmacodynamics of ACT017, an Antiplatelet GPVI (Glycoprotein VI) Fab

(2019) Christine Voors-Pette et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Volumetric Ca2+ Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy

(2019) Siegfried Weisenburger et al.   CELL

High-throughput platelet spreading analysis: a tool for the diagnosis of platelet- based bleeding disorders

(2019) Abdullah O. Khan et al.   HAEMATOLOGICA

Novel Stenotic Microchannels to Study Thrombus Formation in Shear Gradients: Influence of Shear Forces and Human Platelet-Related Factors

(2019) Mathew Lui et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Tubulin in Platelets: When the Shape Matters

(2019) Ernesto José Cuenca-Zamora et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Quantitative Phase Microscopy with Enhanced Contrast and Improved Resolution through Ultra‐Oblique Illumination (UO‐QPM)

(2019) Ying Ma et al.   Journal of Biophotonics

Microclot array elastometry for integrated measurement of thrombus formation and clot biomechanics under fluid shear

(2019) Zhaowei Chen et al.   Nature Communications

Label-free characterization of white blood cells using fluorescence lifetime imaging and flow-cytometry: molecular heterogeneity and erythrophagocytosis [Invited]

(2019) Boris P. Yakimov et al.   Biomedical Optics Express

Platelets and Immune Responses During Thromboinflammation

(2019) Matthias Mezger et al.   Frontiers in Immunology

In vivo monitoring of thrombosis in mice by optical coherence tomography

(2019) Yao Yu et al.   Journal of Biophotonics

Putting advanced microscopy in the hands of biologists

(2019) Rory M. Power et al.   NATURE METHODS

Platelet Mechanotransduction

(2018) Caroline E. Hansen et al.   Annual Review of Biomedical Engineering

Platelets Drive Thrombus Propagation in a Hematocrit and Glycoprotein VI–Dependent Manner in an In Vitro Venous Thrombosis ModelHighlights

(2018) Marcus Lehmann et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Using microfluidic devices to study thrombosis in pathological blood flows

(2018) Bradley A. Herbig et al.   Biomicrofluidics

Mechanisms of platelet clearance and translation to improve platelet storage

(2018) M. Edward Quach et al.   BLOOD

Platelets kill circulating parasites of all major Plasmodium species in human malaria

(2018) Steven Kho et al.   BLOOD

The biology of the platelet with special reference to inflammation wound healing and immunity

(2018) Alan T Nurden   Frontiers in Bioscience-Landmark

Localization Microscopy of Actin Cytoskeleton in Human Platelets

(2018) Sandra Mayr et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

High contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope

(2018) Yongxiao Li et al.   Journal of Biophotonics

Single-shot super-resolution total internal reflection fluorescence microscopy

(2018) Min Guo et al.   NATURE METHODS

STED super-resolved microscopy

(2018) Giuseppe Vicidomini et al.   NATURE METHODS

Structure and function of the open canalicular system – the platelet’s specialized internal membrane network

(2018) Maria V. Selvadurai et al.   PLATELETS

High-throughput measurement of human platelet aggregation under flow: application in hemostasis and beyond

(2018) Sanne L. N. Brouns et al.   PLATELETS

Formin proteins in megakaryocytes and platelets: regulation of actin and microtubule dynamics

(2018) Malou Zuidscherwoude et al.   PLATELETS

Resolution matters: Correlating quantitative proteomics and nanoscale-precision microscopy for reconstructing synapse identity2

(2018) Andras Gabor Miklosi et al.   PROTEOMICS

Atherothrombosis and Thromboembolism: Position Paper from the Second Maastricht Consensus Conference on Thrombosis

(2018) H. Spronk et al.   THROMBOSIS AND HAEMOSTASIS

Diagnostic Potential of Imaging Flow Cytometry

(2018) Minh Doan et al.   TRENDS IN BIOTECHNOLOGY

Morphometric analysis of spread platelets identifies integrin αIIbβ3-specific contractile phenotype

(2018) Sebastian Lickert et al.   Scientific Reports

Dual-color deep-tissue three-photon microscopy with a multiband infrared laser

(2018) Khmaies Guesmi et al.   Light-Science & Applications

Regulation of Leukocytes by TspanC8 Tetraspanins and the “Molecular Scissor” ADAM10

(2018) Alexandra L. Matthews et al.   Frontiers in Immunology

Tracking Cell Recruitment and Behavior within the Tumor Microenvironment Using Advanced Intravital Imaging Approaches

(2018) Madison Turk et al.   Cells

Quantitative phase imaging in biomedicine

(2018) YongKeun Park et al.   Nature Photonics

Multidirectional digital scanned light-sheet microscopy enables uniform fluorescence excitation and contrast-enhanced imaging

(2018) Adam K. Glaser et al.   Scientific Reports

Microfluidic Modeling of Thrombolysis

(2018) Stéphane Loyau et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Structure and dynamics of the platelet integrin-binding C4 domain of von Willebrand factor

(2018) Emma-Ruoqi Xu et al.   BLOOD

Mechanisms of receptor shedding in platelets

(2018) Samantha J. Montague et al.   BLOOD

High-throughput elucidation of thrombus formation reveals sources of platelet function variability

(2018) Johanna P. van Geffen et al.   HAEMATOLOGICA

Super-resolution microscopy demystified

(2018) Lothar Schermelleh et al.   NATURE CELL BIOLOGY

Role of Platelets in Leukocyte Recruitment and Resolution of Inflammation

(2018) Jan Rossaint et al.   Frontiers in Immunology

Shear-mediated platelet activation in the free flow: Perspectives on the emerging spectrum of cell mechanobiological mechanisms mediating cardiovascular implant thrombosis

(2017) Marvin J. Slepian et al.   JOURNAL OF BIOMECHANICS

Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging

(2017) Y. X. Li et al.   Journal of Biophotonics

Actin visualization at a glance

(2017) Michael Melak et al.   JOURNAL OF CELL SCIENCE

Clustering of glycoprotein VI (GPVI) dimers upon adhesion to collagen as a mechanism to regulate GPVI signaling in platelets

(2017) N. S. Poulter et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Hierarchical organization of the hemostatic response to penetrating injuries in the mouse macrovasculature

(2017) J. D. Welsh et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Design, development and characterization of ACT017, a humanized Fab that blocks platelet's glycoprotein VI function without causing bleeding risks

(2017) Kristell Lebozec et al.   mAbs

Imaging flow cytometry in the assessment of leukocyte-platelet aggregates

(2017) Henry Hui et al.   METHODS

Multi-photon microscopy in cardiovascular research

(2017) Zhuojun Wu et al.   METHODS

Hemodynamic studies of platelet thrombosis using microfluidics

(2017) Susan M. Hastings et al.   PLATELETS

Application of microfluidic devices in studies of thrombosis and hemostasis

(2017) Changjie Zhang et al.   PLATELETS

Utility and development of microfluidic platforms for platelet research

(2017) Jevgenia Zilberman-Rudenko et al.   PLATELETS

Flow chamber and microfluidic approaches for measuring thrombus formation in genetic bleeding disorders

(2017) Rogier M. Schoeman et al.   PLATELETS

Use of microfluidics to assess the platelet-based control of coagulation

(2017) Magdolna Nagy et al.   PLATELETS

Neutrophil macroaggregates promote widespread pulmonary thrombosis after gut ischemia

(2017) Yuping Yuan et al.   Science Translational Medicine

Leukocyte integrin Mac-1 regulates thrombosis via interaction with platelet GPIbα

(2017) Yunmei Wang et al.   Nature Communications

Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease

(2017) Walter H. A. Kahr et al.   Nature Communications

A Cdc42/RhoA regulatory circuit downstream of glycoprotein Ib guides transendothelial platelet biogenesis

(2017) Sebastian Dütting et al.   Nature Communications

Thrombopoiesis is spatially regulated by the bone marrow vasculature

(2017) David Stegner et al.   Nature Communications

Platelet Control of Fibrin Distribution and Microelasticity in Thrombus Formation Under FlowSignificance

(2016) Frauke Swieringa et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Synthesis and dephosphorylation of MARCKS in the late stages of megakaryocyte maturation drive proplatelet formation

(2016) K. R. Machlus et al.   BLOOD

Protease-activated receptor 4: from structure to function and back again

(2016) Shauna L French et al.   BRITISH JOURNAL OF PHARMACOLOGY

Executive Summary: Heart Disease and Stroke Statistics—2016 Update

(2016) Dariush Mozaffarian et al.   CIRCULATION

Animal Models of Thrombosis From Zebrafish to Nonhuman Primates

(2016) Pudur Jagadeeswaran et al.   CIRCULATION RESEARCH

Platelets are versatile cells: New discoveries in hemostasis, thrombosis, immune responses, tumor metastasis and beyond

(2016) Xiaohong Ruby Xu et al.   CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES

Extracellular matrix proteins in the regulation of thrombus formation

(2016) Yiming Wang et al.   CURRENT OPINION IN HEMATOLOGY

Seeing is believing – multi-scale spatio-temporal imaging towardsin vivocell biology

(2016) Gautier Follain et al.   JOURNAL OF CELL SCIENCE

Third harmonic generation microscopy of cells and tissue organization

(2016) Bettina Weigelin et al.   JOURNAL OF CELL SCIENCE

Review: imaging technologies for flow cytometry

(2016) Yuanyuan Han et al.   LAB ON A CHIP

Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation

(2016) Elizabeth J. Haining et al.   PLATELETS

The actin binding proteins cortactin and HS1 are dispensable for platelet actin nodule and megakaryocyte podosome formation

(2016) Steven G. Thomas et al.   PLATELETS

Clinical significance of receptor shedding-platelet GPVI as an emerging diagnostic and therapeutic tool

(2016) Madhumita Chatterjee et al.   PLATELETS

Taking the stock of granule cargo: Platelet releasate proteomics

(2016) Oliver Pagel et al.   PLATELETS

The cellular basis of platelet secretion: Emerging structure/function relationships

(2016) Shilpi Yadav et al.   PLATELETS

Red cells’ dynamic morphologies govern blood shear thinning under microcirculatory flow conditions

(2016) Luca Lanotte et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Platelet-mediated modulation of adaptive immunity

(2016) Matteo Iannacone   SEMINARS IN IMMUNOLOGY

Simultaneous multiview capture and fusion improves spatial resolution in wide-field and light-sheet microscopy

(2016) Yicong Wu et al.   Optica

Fluid Mechanics of Blood Clot Formation

(2015) Aaron L. Fogelson et al.   Annual Review of Fluid Mechanics

Platelet-Associated Matrix Metalloproteinases Regulate Thrombus Formation and Exert Local Collagenolytic ActivitySignificance

(2015) Tom G. Mastenbroek et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Platelet secretion: From haemostasis to wound healing and beyond

(2015) Ewelina M. Golebiewska et al.   BLOOD REVIEWS

Accessible Synthetic Probes for Staining Actin inside Platelets and Megakaryocytes by Employing Lifeact Peptide

(2015) Lucia Cardo et al.   CHEMBIOCHEM

Roll, adhere, spread and contract: Structural mechanics of platelet function

(2015) Simona Sorrentino et al.   EUROPEAN JOURNAL OF CELL BIOLOGY

IL-1α induces thrombopoiesis through megakaryocyte rupture in response to acute platelet needs

(2015) Satoshi Nishimura et al.   JOURNAL OF CELL BIOLOGY

Spatiotemporal regulation of coagulation and platelet activation during the hemostatic responsein vivo

(2015) L. Ivanciu et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Microfluidic technology as an emerging clinical tool to evaluate thrombosis and hemostasis

(2015) Brian R. Branchford et al.   THROMBOSIS RESEARCH

Platelet actin nodules are podosome-like structures dependent on Wiskott–Aldrich syndrome protein and ARP2/3 complex

(2015) Natalie S. Poulter et al.   Nature Communications

Quantitative analysis of platelets aggregates in 3D by digital holographic microscopy

(2015) Karim Zouaoui Boudejltia et al.   Biomedical Optics Express

Multicolor Fluorescence Nanoscopy by Photobleaching: Concept, Verification, and Its Application To Resolve Selective Storage of Proteins in Platelets

(2014) Daniel Rönnlund et al.   ACS Nano

A systems approach to hemostasis: 1. The interdependence of thrombus architecture and agonist movements in the gaps between platelets

(2014) J. D. Welsh et al.   BLOOD

Src family kinases: at the forefront of platelet activation

(2014) Y. A. Senis et al.   BLOOD

Quantitative proteomics analysis reveals similar release profiles following specific PAR-1 or PAR-4 stimulation of platelets

(2014) Thijs C. van Holten et al.   CARDIOVASCULAR RESEARCH

Measurement of monocyte-platelet aggregates by imaging flow cytometry

(2014) Henry Hui et al.   CYTOMETRY PART A

Primary haemostasis: newer insights

(2014) M. C. Berndt et al.   HAEMOPHILIA

Characterization of the platelet granule proteome: Evidence of the presence of MHC1 in alpha-granules

(2014) Anne Zufferey et al.   Journal of Proteomics

High-resolution reconstruction of the beating zebrafish heart

(2014) Michaela Mickoleit et al.   NATURE METHODS

Dynamic analysis of platelet deposition to resolve platelet adhesion receptor activity in whole blood at arterial shear rate

(2014) Nicholas Pugh et al.   PLATELETS

Platelet demand modulates the type of intravascular protrusion of megakaryocytes in bone marrow

(2014) Shugo Kowata et al.   THROMBOSIS AND HAEMOSTASIS

Platelet Receptor Expression and Shedding: Glycoprotein Ib-IX-V and Glycoprotein VI

(2014) Elizabeth E. Gardiner et al.   TRANSFUSION MEDICINE REVIEWS

Identification of platelet function defects by multi-parameter assessment of thrombus formation

(2014) Susanne M. de Witt et al.   Nature Communications

Geometric design of microfluidic chambers: platelet adhesion versus accumulation

(2013) Lauren D. C. Casa et al.   BIOMEDICAL MICRODEVICES

Hierarchical organization in the hemostatic response and its relationship to the platelet-signaling network

(2013) T. J. Stalker et al.   BLOOD

Reporter Mouse Lines for Fluorescence Imaging

(2013) Takaya Abe et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Quantification of volume, mass, and density of thrombus formation using brightfield and differential interference contrast microscopy

(2013) Sandra M. Baker-Groberg et al.   JOURNAL OF BIOMEDICAL OPTICS

Coherent anti-Stokes Raman scattering microscopy: overcoming technical barriers for clinical translation

(2013) Haohua Tu et al.   Journal of Biophotonics

Genotyping and phenotyping of platelet function disorders

(2013) S. P. Watson et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

A directional switch of integrin signalling and a new anti-thrombotic strategy

(2013) Bo Shen et al.   NATURE

Formation of microvascular networks in vitro

(2013) John P Morgan et al.   Nature Protocols

Cathepsin G-Dependent Modulation of Platelet Thrombus Formation In Vivo by Blood Neutrophils

(2013) Nauder Faraday et al.   PLoS One

Quantitative Phase Imaging Techniques for the Study of Cell Pathophysiology: From Principles to Applications

(2013) KyeoReh Lee et al.   SENSORS

Differentiating haemostasis from thrombosis for therapeutic benefit

(2013) James D. McFadyen et al.   THROMBOSIS AND HAEMOSTASIS

From seeing to believing: labelling strategies for in vivo cell-tracking experiments

(2013) F. Progatzky et al.   Interface Focus

Cadherin 6 Has a Functional Role in Platelet Aggregation and Thrombus Formation

(2012) Eimear Dunne et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Differential Dynamics of Platelet Contact and Spreading

(2012) Dooyoung Lee et al.   BIOPHYSICAL JOURNAL

Increased platelet activation and thrombosis in transgenic mice expressing constitutively active P2Y12

(2012) Y. ZHANG et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Rho GTPases in platelet function

(2012) J. E. ASLAN et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure

(2012) Xiyi Chen et al.   Nature Protocols

Monitoringin vitrothrombus formation with novel microfluidic devices

(2012) Erik Westein et al.   PLATELETS

In vitro microvessels for the study of angiogenesis and thrombosis

(2012) Y. Zheng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Thrombocytopenia resulting from mutations in filamin A can be expressed as an isolated syndrome

(2011) P. Nurden et al.   BLOOD

In vivo flow cytometry: A horizon of opportunities

(2011) Valery V. Tuchin et al.   CYTOMETRY PART A

The maternal immune response to fetal platelet GPIbα causes frequent miscarriage in mice that can be prevented by intravenous IgG and anti-FcRn therapies

(2011) Conglei Li et al.   JOURNAL OF CLINICAL INVESTIGATION

Arterial thrombosis—insidious, unpredictable and deadly

(2011) Shaun P Jackson   NATURE MEDICINE

Measuring and interpreting point spread functions to determine confocal microscope resolution and ensure quality control

(2011) Richard W Cole et al.   Nature Protocols

The Use of Spinning-Disk Confocal Microscopy for the Intravital Analysis of Platelet Dynamics in Response to Systemic and Local Inflammation

(2011) Craig N. Jenne et al.   PLoS One

von Willebrand factor-mediated platelet adhesion is critical for deep vein thrombosis in mouse models

(2010) A. Brill et al.   BLOOD

Determinants of platelet count in humans

(2010) M. E. Daly   HAEMATOLOGICA

Two-photon intravital imaging of thrombus development

(2010) Malgorzata M. Kamocka et al.   JOURNAL OF BIOMEDICAL OPTICS

Cytoskeletal mechanics of proplatelet maturation and platelet release

(2010) Jonathan N. Thon et al.   JOURNAL OF CELL BIOLOGY

Imaging with total internal reflection fluorescence microscopy for the cell biologist

(2010) A. L. Mattheyses et al.   JOURNAL OF CELL SCIENCE

Going deeper than microscopy: the optical imaging frontier in biology

(2010) Vasilis Ntziachristos   NATURE METHODS

GPIbα-selective activation of platelets induces platelet signaling events comparable to GPVI activation events

(2010) Elizabeth E. Gardiner et al.   PLATELETS

Super-Resolution Fluorescence Microscopy

(2009) Bo Huang et al.   Annual Review of Biochemistry

Matrix Crosslinking Forces Tumor Progression by Enhancing Integrin Signaling

(2009) Kandice R. Levental et al.   CELL

Lnk regulates integrin αIIbβ3 outside-in signaling in mouse platelets, leading to stabilization of thrombus development in vivo

(2009) Hitoshi Takizawa et al.   JOURNAL OF CLINICAL INVESTIGATION

A shear gradient–dependent platelet aggregation mechanism drives thrombus formation

(2009) Warwick S Nesbitt et al.   NATURE MEDICINE

Absence of platelet-dependent fibrin formation in a patient with Scott syndrome

(2009) Simone J. H. Wielders et al.   THROMBOSIS AND HAEMOSTASIS

In vivo multispectral, multiparameter, photoacoustic lymph flow cytometry with natural cell focusing, label-free detection and multicolor nanoparticle probes

(2008) Ekaterina I. Galanzha et al.   CYTOMETRY PART A

In vivo imaging in mice reveals local cell dynamics and inflammation in obese adipose tissue

(2008) Satoshi Nishimura et al.   JOURNAL OF CLINICAL INVESTIGATION

Triggers, targets and treatments for thrombosis

(2008) Nigel Mackman   NATURE

Lifeact: a versatile marker to visualize F-actin

(2008) Julia Riedl et al.   NATURE METHODS

Mechanisms of Thrombus Formation

(2008) Bruce Furie et al.   NEW ENGLAND JOURNAL OF MEDICINE

Prothrombotic Effects of Fibronectin Isoforms Containing the EDA Domain

(2007) Anil K. Chauhan et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY