Seeing is believing – multi-scale spatio-temporal imaging towardsin vivocell biology

A 3-D cell culture system to study epithelia functions using microcarriers

(2016) Petra H. Jakob et al.   CYTOTECHNOLOGY

Measuring forces and stressesin situin living tissues

(2016) Kaoru Sugimura et al.   DEVELOPMENT

Real-Time Three-Dimensional Cell Segmentation in Large-Scale Microscopy Data of Developing Embryos

(2016) Johannes Stegmaier et al.   DEVELOPMENTAL CELL

Quantitative Multiscale Cell Imaging in Controlled 3D Microenvironments

(2016) Erik S. Welf et al.   DEVELOPMENTAL CELL

Third harmonic generation microscopy of cells and tissue organization

(2016) Bettina Weigelin et al.   JOURNAL OF CELL SCIENCE

Generating and characterizing the mechanical properties of cell-derived matrices using atomic force microscopy

(2016) Marta Tello et al.   METHODS

Using magnets and magnetic beads to dissect signaling pathways activated by mechanical tension applied to cells

(2016) R.J. Marjoram et al.   METHODS

Visualization of immediate immune responses to pioneer metastatic cells in the lung

(2016) Mark B. Headley et al.   NATURE

High-density three-dimensional localization microscopy across large volumes

(2016) Wesley R Legant et al.   NATURE METHODS

Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT

(2016) Patrick Hoyer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ESCRT III repairs nuclear envelope ruptures during cell migration to limit DNA damage and cell death

(2016) M. Raab et al.   SCIENCE

Perinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments

(2016) Hawa-Racine Thiam et al.   Nature Communications

Two-Photon Excitation STED Microscopy with Time-Gated Detection

(2016) Iván Coto Hernández et al.   Scientific Reports

Single-cell mechanics – An experimental–computational method for quantifying the membrane–cytoskeleton elasticity of cells

(2015) M. Tartibi et al.   Acta Biomaterialia

Active cell mechanics: Measurement and theory

(2015) Wylie W. Ahmed et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Traction force microscopy on soft elastic substrates: A guide to recent computational advances

(2015) Ulrich S. Schwarz et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Colored polydimethylsiloxane micropillar arrays for high throughput measurements of forces applied by genetic model organisms

(2015) Siddharth M. Khare et al.   Biomicrofluidics

Traction Forces of Endothelial Cells under Slow Shear Flow

(2015) Cecile M. Perrault et al.   BIOPHYSICAL JOURNAL

In Vivo Imaging Reveals Extracellular Vesicle-Mediated Phenocopying of Metastatic Behavior

(2015) Anoek Zomer et al.   CELL

Confinement and Low Adhesion Induce Fast Amoeboid Migration of Slow Mesenchymal Cells

(2015) Yan-Jun Liu et al.   CELL

Light sheet-based fluorescence microscopy (LSFM) for the quantitative imaging of cells and tissues

(2015) Francesco Pampaloni et al.   CELL AND TISSUE RESEARCH

Proteomics of Primary Cilia by Proximity Labeling

(2015) David U. Mick et al.   DEVELOPMENTAL CELL

An Organized Co-assembly of Clathrin Adaptors Is Essential for Endocytosis

(2015) Michal Skruzny et al.   DEVELOPMENTAL CELL

High-resolution live imaging reveals axon-glia interactions during peripheral nerve injury and repair in zebrafish

(2015) Y. Xiao et al.   Disease Models & Mechanisms

Axial resolution beyond the diffraction limit of a sheet illumination microscope with stimulated emission depletion

(2015) Mike Friedrich et al.   JOURNAL OF BIOMEDICAL OPTICS

Three-dimensional tracking of plus-tips by lattice light-sheet microscopy permits the quantification of microtubule growth trajectories within the mitotic apparatus

(2015) Norio Yamashita et al.   JOURNAL OF BIOMEDICAL OPTICS

RAL-1 controls multivesicular body biogenesis and exosome secretion

(2015) Vincent Hyenne et al.   JOURNAL OF CELL BIOLOGY

Fast and precise targeting of single tumor cellsin vivoby multimodal correlative microscopy

(2015) Matthia A. Karreman et al.   JOURNAL OF CELL SCIENCE

Correlative light and electron microscopy enables viral replication studies at the ultrastructural level

(2015) Kirsi Hellström et al.   METHODS

Force-Controlled Patch Clamp of Beating Cardiac Cells

(2015) Dario Ossola et al.   NANO LETTERS

Ultrafast, temporally stochastic STED nanoscopy of millisecond dynamics

(2015) Jale Schneider et al.   NATURE METHODS

Whole-brain functional imaging with two-photon light-sheet microscopy

(2015) Sébastien Wolf et al.   NATURE METHODS

Fixation-resistant photoactivatable fluorescent proteins for CLEM

(2015) Maria G Paez-Segala et al.   NATURE METHODS

Whole-animal functional and developmental imaging with isotropic spatial resolution

(2015) Raghav K Chhetri et al.   NATURE METHODS

Correlated light and electron microscopy: ultrastructure lights up!

(2015) Pascal de Boer et al.   NATURE METHODS

A dynamic DNA-repair complex observed by correlative single-molecule nanomanipulation and fluorescence

(2015) Evan T Graves et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Visualizing Whole-Brain Activity and Development at the Single-Cell Level Using Light-Sheet Microscopy

(2015) Philipp J. Keller et al.   NEURON

Direct laser manipulation reveals the mechanics of cell contacts in vivo

(2015) Kapil Bambardekar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Whole-brain calcium imaging with cellular resolution in freely behavingCaenorhabditis elegans

(2015) Jeffrey P. Nguyen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics

(2015) D. Li et al.   SCIENCE

Endocytic sites mature by continuous bending and remodeling of the clathrin coat

(2015) O. Avinoam et al.   SCIENCE

A dynamic DNA-repair complex observed by correlative single-molecule nanomanipulation and fluorescence

(2015) Evan T Graves et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Mechanical interplay between invadopodia and the nucleus in cultured cancer cells

(2015) Or-Yam Revach et al.   Scientific Reports

The Cardiomyopathy Lamin A/C D192G Mutation Disrupts Whole-Cell Biomechanics in Cardiomyocytes as Measured by Atomic Force Microscopy Loading-Unloading Curve Analysis

(2015) Thomas Lanzicher et al.   Scientific Reports

Super-resolution mapping of glutamate receptors in C. elegans by confocal correlated PALM

(2015) Jeroen Vangindertael et al.   Scientific Reports

Developmental Alterations in Heart Biomechanics and Skeletal Muscle Function in Desmin Mutants Suggest an Early Pathological Root for Desminopathies

(2015) Caroline Ramspacher et al.   Cell Reports

Mechanical Coupling between Endoderm Invagination and Axis Extension in Drosophila

(2015) Claire M. Lye et al.   PLOS BIOLOGY

Light sheet fluorescence microscopy (LSFM): past, present and future

(2014) John Lim et al.   ANALYST

Nanoscopy of Filamentous Actin in Cortical Dendrites of a Living Mouse

(2014) Katrin I. Willig et al.   BIOPHYSICAL JOURNAL

The cancer glycocalyx mechanically primes integrin-mediated growth and survival

(2014) Matthew J. Paszek et al.   NATURE

Centriole amplification by mother and daughter centrioles differs in multiciliated cells

(2014) Adel Al Jord et al.   NATURE

Intestinal crypt homeostasis revealed at single-stem-cell level by in vivo live imaging

(2014) Laila Ritsma et al.   NATURE

Directed evolution of APEX2 for electron microscopy and proximity labeling

(2014) Stephanie S Lam et al.   NATURE METHODS

High-resolution reconstruction of the beating zebrafish heart

(2014) Michaela Mickoleit et al.   NATURE METHODS

STED-SPIM made simple

(2014) Teodora Scheul et al.   OPTICS EXPRESS

Correlating Intravital Multi-Photon Microscopy to 3D Electron Microscopy of Invading Tumor Cells Using Anatomical Reference Points

(2014) Matthia A. Karreman et al.   PLoS One

Whole-cell, multicolor superresolution imaging using volumetric multifocus microscopy

(2014) Bassam Hajj et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution

(2014) Bi-Chang Chen et al.   SCIENCE

Haemodynamic and extracellular matrix cues regulate the mechanical phenotype and stiffness of aortic endothelial cells

(2014) Caitlin Collins et al.   Nature Communications

Endothelial Cilia Mediate Low Flow Sensing during Zebrafish Vascular Development

(2014) Jacky G. Goetz et al.   Cell Reports

Live-Cell Superresolution Imaging by Pulsed STED Two-Photon Excitation Microscopy

(2013) Kevin T. Takasaki et al.   BIOPHYSICAL JOURNAL

Heartbeat-Driven Pericardiac Fluid Forces Contribute to Epicardium Morphogenesis

(2013) Marina Peralta et al.   CURRENT BIOLOGY

Principles of E-Cadherin Supramolecular Organization In Vivo

(2013) Binh-An Truong Quang et al.   CURRENT BIOLOGY

Preclinical intravital microscopy of the tumour-stroma interface: invasion, metastasis, and therapy response

(2013) Stephanie Alexander et al.   CURRENT OPINION IN CELL BIOLOGY

Pulse propagation by a capacitive mechanism drives embryonic blood flow

(2013) H. Anton et al.   DEVELOPMENT

A pathway for unicellular tube extension depending on the lymphatic vessel determinant Prox1 and on osmoregulation

(2013) Irina Kolotuev et al.   NATURE CELL BIOLOGY

Whole-brain functional imaging at cellular resolution using light-sheet microscopy

(2013) Misha B Ahrens et al.   NATURE METHODS

Instant super-resolution imaging in live cells and embryos via analog image processing

(2013) Andrew G York et al.   NATURE METHODS

Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms

(2013) Fang Huang et al.   NATURE METHODS

Correlative In Vivo 2 Photon and Focused Ion Beam Scanning Electron Microscopy of Cortical Neurons

(2013) Bohumil Maco et al.   PLoS One

In vivo single branch axotomy induces GAP-43-dependent sprouting and synaptic remodeling in cerebellar cortex

(2013) A. L. Allegra Mascaro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging

(2013) Togo Shimozawa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Confocal microscopy for astrocyte in vivo imaging: Recycle and reuse in microscopy

(2013) Alberto Pérez-Alvarez et al.   Frontiers in Cellular Neuroscience

Trapping red blood cells in living animals using optical tweezers

(2013) Min-Cheng Zhong et al.   Nature Communications

Evolutionary conservation of early mesoderm specification by mechanotransduction in Bilateria

(2013) Thibaut Brunet et al.   Nature Communications

Plasma Membrane Reshaping during Endocytosis Is Revealed by Time-Resolved Electron Tomography

(2012) Wanda Kukulski et al.   CELL

Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens

(2012) Liang Gao et al.   CELL

Integrins β1 and β3 exhibit distinct dynamic nanoscale organizations inside focal adhesions

(2012) Olivier Rossier et al.   NATURE CELL BIOLOGY

Multiview light-sheet microscope for rapid in toto imaging

(2012) Uros Krzic et al.   NATURE METHODS

Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy

(2012) Andrew G York et al.   NATURE METHODS

Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy

(2012) Raju Tomer et al.   NATURE METHODS

The nanomechanical signature of breast cancer

(2012) Marija Plodinec et al.   Nature Nanotechnology

Intravital multiphoton imaging of immune responses in the mouse ear skin

(2012) Jackson LiangYao Li et al.   Nature Protocols

Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination

(2012) R. Fiolka et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nanoscopy in a Living Mouse Brain

(2012) S. Berning et al.   SCIENCE

Intravital Microscopy Through an Abdominal Imaging Window Reveals a Pre-Micrometastasis Stage During Liver Metastasis

(2012) L. Ritsma et al.   Science Translational Medicine

Spatial coordination between cell and nuclear shape within micropatterned endothelial cells

(2012) Marie Versaevel et al.   Nature Communications

Nanoscopy in a Living Multicellular Organism Expressing GFP

(2011) Brian R. Rankin et al.   BIOPHYSICAL JOURNAL

STED-SPIM: Stimulated Emission Depletion Improves Sheet Illumination Microscopy Resolution

(2011) Mike Friedrich et al.   BIOPHYSICAL JOURNAL

Light sheet microscopy for real-time developmental biology

(2011) Michael Weber et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

A Hitchhiker's Guide to Mechanobiology

(2011) Jeroen Eyckmans et al.   DEVELOPMENTAL CELL

In vivo two-photon microscopy to 1.6-mm depth in mouse cortex

(2011) Demirhan Kobat et al.   JOURNAL OF BIOMEDICAL OPTICS

Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision

(2011) Wanda Kukulski et al.   JOURNAL OF CELL BIOLOGY

Dynamic molecular processes mediate cellular mechanotransduction

(2011) Brenton D. Hoffman et al.   NATURE

Deep and fast live imaging with two-photon scanned light-sheet microscopy

(2011) Thai V Truong et al.   NATURE METHODS

Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination

(2011) Thomas A Planchon et al.   NATURE METHODS

Sharper low-power STED nanoscopy by time gating

(2011) Giuseppe Vicidomini et al.   NATURE METHODS

Near-infrared branding efficiently correlates light and electron microscopy

(2011) Derron Bishop et al.   NATURE METHODS

Super-resolution 3D microscopy of live whole cells using structured illumination

(2011) Lin Shao et al.   NATURE METHODS

The Use of Spinning-Disk Confocal Microscopy for the Intravital Analysis of Platelet Dynamics in Response to Systemic and Local Inflammation

(2011) Craig N. Jenne et al.   PLoS One

Mechanosensitive shivering of model tissues under controlled aspiration

(2011) K. Guevorkian et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution

(2011) E. H. Rego et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Imaging with second-harmonic radiation probes in living tissue

(2011) Rachel Grange et al.   Biomedical Optics Express

A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms

(2011) Xiaokun Shu et al.   PLOS BIOLOGY

Mechanisms of mechanical signaling in development and disease

(2010) P. A. Janmey et al.   JOURNAL OF CELL SCIENCE

DNA Binding to an Anticancer Organo-Ruthenium Complex

(2010) Marcelina Klajner et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Blood stem cells emerge from aortic endothelium by a novel type of cell transition

(2010) Karima Kissa et al.   NATURE

Protein localization in electron micrographs using fluorescence nanoscopy

(2010) Shigeki Watanabe et al.   NATURE METHODS

Magnetic tweezers measurements of the nanomechanical properties of DNA in the presence of drugs

(2010) D. Salerno et al.   NUCLEIC ACIDS RESEARCH

Aspiration of Biological Viscoelastic Drops

(2010) Karine Guevorkian et al.   PHYSICAL REVIEW LETTERS

From Dynamic Live Cell Imaging to 3D Ultrastructure: Novel Integrated Methods for High Pressure Freezing and Correlative Light-Electron Microscopy

(2010) Coralie Spiegelhalter et al.   PLoS One

Second harmonic generating (SHG) nanoprobes for in vivo imaging

(2010) P. Pantazis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Microengineered Platforms for Cell Mechanobiology

(2009) Deok-Ho Kim et al.   Annual Review of Biomedical Engineering

In vivo nanomechanical imaging of blood-vessel tissues directly in living mammals using atomic force microscopy

(2009) Youdong Mao et al.   APPLIED PHYSICS LETTERS

A precise and rapid mapping protocol for correlative light and electron microscopy of small invertebrate organisms

(2009) Irina Kolotuev et al.   BIOLOGY OF THE CELL

Real-time imaging reveals the single steps of brain metastasis formation

(2009) Yvonne Kienast et al.   NATURE MEDICINE

Super-resolution video microscopy of live cells by structured illumination

(2009) Peter Kner et al.   NATURE METHODS

Subdiffraction-Resolution Fluorescence Imaging with Conventional Fluorescent Probes

(2008) Mike Heilemann et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Endothelial cell cytoskeletal alignment independent of fluid shear stress on micropatterned surfaces

(2008) Keri B. Vartanian et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Three-Dimensional Resolution Doubling in Wide-Field Fluorescence Microscopy by Structured Illumination

(2008) Mats G.L. Gustafsson et al.   BIOPHYSICAL JOURNAL

Tissue Deformation Modulates Twist Expression to Determine Anterior Midgut Differentiation in Drosophila Embryos

(2008) Nicolas Desprat et al.   DEVELOPMENTAL CELL

Light sheet‐based fluorescence microscopy: More dimensions, more photons, and less photodamage

(2008) Emmanuel G. Reynaud et al.   HFSP Journal

Three-dimensional sub–100 nm resolution fluorescence microscopy of thick samples

(2008) Manuel F Juette et al.   NATURE METHODS

High-density mapping of single-molecule trajectories with photoactivated localization microscopy

(2008) Suliana Manley et al.   NATURE METHODS

Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy

(2008) Keir C Neuman et al.   NATURE METHODS

Video-Rate Far-Field Optical Nanoscopy Dissects Synaptic Vesicle Movement

(2008) V. Westphal et al.   SCIENCE

Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy

(2008) P. J. Keller et al.   SCIENCE

A Single Method for Cryofixation and Correlative Light, Electron Microscopy and Tomography of Zebrafish Embryos

(2008) Susan J. Nixon et al.   TRAFFIC