Deep Profiling of Cellular Heterogeneity by Emerging Single‐Cell Proteomic Technologies

Single-Cell Omics Analyses Enabled by Microchip Technologies

(2019) Yanxiang Deng et al.   Annual Review of Biomedical Engineering

Single-Cell Protein Secretion Detection and Profiling

(2019) Zhuo Chen et al.   Annual Review of Analytical Chemistry

Phenotypic heterogeneity and evolution of melanoma cells associated with targeted therapy resistance

(2019) Yapeng Su et al.   PLoS Computational Biology

A dream of single-cell proteomics

(2019) Vivien Marx   NATURE METHODS

Ultrasimple Single-Cell Detection of Multiple Cytokines by a Nanowell Chip Integrated with Encoded Microarrays

(2019) Mohammed A. A. Abdullah et al.   ACS Sensors

Single-Cell Mass Spectrometry Approaches to Explore Cellular Heterogeneity

(2018) Linwen Zhang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Highly Multiplexed Single-Cell Protein Analysis

(2018) Manas Mondal et al.   CHEMISTRY-A EUROPEAN JOURNAL

Transformative Opportunities for Single-Cell Proteomics

(2018) Harrison Specht et al.   JOURNAL OF PROTEOME RESEARCH

Highly multiplexed single-cell in situ RNA and DNA analysis with bioorthogonal cleavable fluorescent oligonucleotides

(2018) Manas Mondal et al.   Chemical Science

Metal-isotope-tagged monoclonal antibodies for high-dimensional mass cytometry

(2018) Guojun Han et al.   Nature Protocols

Highly Multiplexed Single-Cell Protein Profiling with Large-Scale Convertible DNA-Antibody Barcoded Arrays

(2018) Peng Zhao et al.   Advanced Science

Quantifying Homologous Proteins and Proteoforms

(2018) Dmitry Malioutov et al.   MOLECULAR & CELLULAR PROTEOMICS

Tumour heterogeneity and metastasis at single-cell resolution

(2018) Devon A. Lawson et al.   NATURE CELL BIOLOGY

Single-cell proteomics

(2018) Allison Doerr   NATURE METHODS

Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens

(2018) Randy J. Giedt et al.   Nature Communications

Mass cytometry identifies a distinct monocyte cytokine signature shared by clinically heterogeneous pediatric SLE patients

(2017) W.E. O'Gorman et al.   JOURNAL OF AUTOIMMUNITY

Microchip-based single-cell functional proteomics for biomedical applications

(2017) Yao Lu et al.   LAB ON A CHIP

High-throughput screening of rare metabolically active tumor cells in pleural effusion and peripheral blood of lung cancer patients

(2017) Yin Tang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-cell analysis resolves the cell state transition and signaling dynamics associated with melanoma drug-induced resistance

(2017) Yapeng Su et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A subcellular map of the human proteome

(2017) Peter J. Thul et al.   SCIENCE

Profiling protein expression in circulating tumour cells using microfluidic western blotting

(2017) Elly Sinkala et al.   Nature Communications

Single Muscle Fiber Proteomics Reveals Fiber-Type-Specific Features of Human Muscle Aging

(2017) Marta Murgia et al.   Cell Reports

Mass Cytometry Identifies Distinct Lung CD4+ T Cell Patterns in Löfgren’s Syndrome and Non-Löfgren’s Syndrome Sarcoidosis

(2017) Ylva Kaiser et al.   Frontiers in Immunology

Subcellular western blotting of single cells

(2017) Kevin A. Yamauchi et al.   Microsystems & Nanoengineering

Single-Cell, Multiplexed Protein Detection of Rare Tumor Cells Based on a Beads-on-Barcode Antibody Microarray

(2016) Liu Yang et al.   ANALYTICAL CHEMISTRY

Recent Advances in the Analysis of Single Cells

(2016) Lucas Armbrecht et al.   ANALYTICAL CHEMISTRY

Single-Cell Mass Spectrometry for Discovery Proteomics: Quantifying Translational Cell Heterogeneity in the 16-Cell Frog (Xenopus ) Embryo

(2016) Camille Lombard-Banek et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Single-Cell (Multi)omics Technologies

(2016) Lia Chappell et al.   Annual Review of Genomics and Human Genetics

Mass Cytometry: Single Cells, Many Features

(2016) Matthew H. Spitzer et al.   CELL

Emerging single-cell technologies for functional proteomics in oncology

(2016) Jun Wang et al.   Expert Review of Proteomics

Mapping the Fetomaternal Peripheral Immune System at Term Pregnancy

(2016) Gabriela K. Fragiadakis et al.   JOURNAL OF IMMUNOLOGY

Identification of Maturation-Specific Proteins by Single-Cell Proteomics of Human Oocytes

(2016) Irma Virant-Klun et al.   MOLECULAR & CELLULAR PROTEOMICS

Mass cytometry: blessed with the curse of dimensionality

(2016) Evan W Newell et al.   NATURE IMMUNOLOGY

Highly multiplexed simultaneous detection of RNAs and proteins in single cells

(2016) Andreas P Frei et al.   NATURE METHODS

Single cell–resolution western blotting

(2016) Chi-Chih Kang et al.   Nature Protocols

High-Content Quantification of Single-Cell Immune Dynamics

(2016) Michael Junkin et al.   Cell Reports

The Size of the Human Proteome: The Width and Depth

(2016) Elena A. Ponomarenko et al.   International Journal of Analytical Chemistry

Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting

(2015) Todd A. Duncombe et al.   ADVANCED MATERIALS

Data-Driven Phenotypic Dissection of AML Reveals Progenitor-like Cells that Correlate with Prognosis

(2015) Jacob H. Levine et al.   CELL

Single muscle fiber proteomics reveals unexpected mitochondrial specialization

(2015) M. Murgia et al.   EMBO REPORTS

An Integrated Platform for Isolation, Processing, and Mass Spectrometry-based Proteomic Profiling of Rare Cells in Whole Blood

(2015) Siyang Li et al.   MOLECULAR & CELLULAR PROTEOMICS

Single-cell analysis tools for drug discovery and development

(2015) James R. Heath et al.   NATURE REVIEWS DRUG DISCOVERY

Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands

(2015) Yao Lu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Analysis of single-cell cytokine secretion reveals a role for paracrine signaling in coordinating macrophage responses to TLR4 stimulation

(2015) Qiong Xue et al.   Science Signaling

Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method

(2015) Jia-Ren Lin et al.   Nature Communications

Analysis of single-cell cytokine secretion reveals a role for paracrine signaling in coordinating macrophage responses to TLR4 stimulation

(2015) Qiong Xue et al.   Science Signaling

Single-Cell Western Blotting after Whole-Cell Imaging to Assess Cancer Chemotherapeutic Response

(2014) Chi-Chih Kang et al.   ANALYTICAL CHEMISTRY

Microfluidics-Based Single-Cell Functional Proteomics for Fundamental and Applied Biomedical Applications

(2014) Jing Yu et al.   Annual Review of Analytical Chemistry

Proteogenomics: concepts, applications and computational strategies

(2014) Alexey I Nesvizhskii   NATURE METHODS

Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry

(2014) Charlotte Giesen et al.   NATURE METHODS

Single-cell western blotting

(2014) Alex J Hughes et al.   NATURE METHODS

Glioblastoma cellular architectures are predicted through the characterization of two-cell interactions

(2014) N. Kravchenko-Balasha et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cancer Cell Profiling by Barcoding Allows Multiplexed Protein Analysis in Fine-Needle Aspirates

(2014) A. V. Ullal et al.   Science Translational Medicine

An Integrated Microfluidic Chip System for Single-Cell Secretion Profiling of Rare Circulating Tumor Cells

(2014) Yuliang Deng et al.   Scientific Reports

High-Throughput Secretomic Analysis of Single Cells to Assess Functional Cellular Heterogeneity

(2013) Yao Lu et al.   ANALYTICAL CHEMISTRY

Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics

(2013) Venkatachalam Chokkalingam et al.   LAB ON A CHIP

viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia

(2013) El-ad David Amir et al.   NATURE BIOTECHNOLOGY

Single-cell analysis and sorting using droplet-based microfluidics

(2013) Linas Mazutis et al.   Nature Protocols

Hypoxia induces a phase transition within a kinase signaling network in cancer cells

(2013) W. Wei et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue

(2013) M. J. Gerdes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genetic and Environmental Determinants of Human NK Cell Diversity Revealed by Mass Cytometry

(2013) A. Horowitz et al.   Science Translational Medicine

Quantum dot imaging platform for single-cell molecular profiling

(2013) Pavel Zrazhevskiy et al.   Nature Communications

Cellular Barcodes for Efficiently Profiling Single-Cell Secretory Responses by Microengraving

(2012) Yvonne J. Yamanaka et al.   ANALYTICAL CHEMISTRY

Multiplexed In Situ Immunofluorescence Using Dynamic DNA Complexes

(2012) Ryan M. Schweller et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cellular Heterogeneity and Molecular Evolution in Cancer

(2012) Vanessa Almendro et al.   Annual Review of Pathology-Mechanisms of Disease

Cytometry by Time-of-Flight Shows Combinatorial Cytokine Expression and Virus-Specific Cell Niches within a Continuum of CD8+ T Cell Phenotypes

(2012) Evan W. Newell et al.   IMMUNITY

Photocleavable DNA Barcode–Antibody Conjugates Allow Sensitive and Multiplexed Protein Analysis in Single Cells

(2012) Sarit S. Agasti et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Droplet microfluidics for high-throughput biological assays

(2012) Mira T. Guo et al.   LAB ON A CHIP

Functional single-cell analysis of T-cell activation by supported lipid bilayer-tethered ligands on arrays of nanowells

(2012) Alexis J. Torres et al.   LAB ON A CHIP

Quantitating Cell–Cell Interaction Functions with Applications to Glioblastoma Multiforme Cancer Cells

(2012) Jun Wang et al.   NANO LETTERS

Multiplexed mass cytometry profiling of cellular states perturbed by small-molecule regulators

(2012) Bernd Bodenmiller et al.   NATURE BIOTECHNOLOGY

Rapid, efficient functional characterization and recovery of HIV-specific human CD8+ T cells using microengraving

(2012) N. Varadarajan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Functional single-cell hybridoma screening using droplet-based microfluidics

(2012) B. E. Debs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A deep profiler's guide to cytometry

(2012) Sean C. Bendall et al.   TRENDS IN IMMUNOLOGY

Single-cell protein analysis

(2011) Meiye Wu et al.   CURRENT OPINION IN BIOTECHNOLOGY

Microfluidics for single cell analysis

(2011) Huabing Yin et al.   CURRENT OPINION IN BIOTECHNOLOGY

A high-throughput single-cell analysis of human CD8+ T cell functions reveals discordance for cytokine secretion and cytolysis

(2011) Navin Varadarajan et al.   JOURNAL OF CLINICAL INVESTIGATION

A clinical microchip for evaluation of single immune cells reveals high functional heterogeneity in phenotypically similar T cells

(2011) Chao Ma et al.   NATURE MEDICINE

Single-cell proteomic chip for profiling intracellular signaling pathways in single tumor cells

(2011) Q. Shi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Polyfunctional responses by human T cells result from sequential release of cytokines

(2011) Q. Han et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum

(2011) S. C. Bendall et al.   SCIENCE

Multiplexed and Reiterative Fluorescence Labeling via DNA Circuitry

(2010) Dzifa Y. Duose et al.   BIOCONJUGATE CHEMISTRY

Genetic Heterogeneity in Human Disease

(2010) Jon McClellan et al.   CELL

Cellular Heterogeneity: Do Differences Make a Difference?

(2010) Steven J. Altschuler et al.   CELL

Chemistries for Patterning Robust DNA MicroBarcodes Enable Multiplex Assays of Cytoplasm Proteins from Single Cancer Cells

(2010) Young Shik Shin et al.   CHEMPHYSCHEM

Multidimensional analysis of the frequencies and rates of cytokine secretion from single cells by quantitative microengraving

(2010) Qing Han et al.   LAB ON A CHIP

Measurement of single-cell dynamics

(2010) David G. Spiller et al.   NATURE

Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells

(2010) Y. Taniguchi et al.   SCIENCE

Single cell analysis: the new frontier in ‘omics’

(2010) Daojing Wang et al.   TRENDS IN BIOTECHNOLOGY

Droplet microfluidic technology for single-cell high-throughput screening

(2009) E. Brouzes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A microfluidic device for multiplexed protein detection in nano-liter volumes

(2008) Alan H. Diercks et al.   ANALYTICAL BIOCHEMISTRY

Concurrent detection of secreted products from human lymphocytes by microengraving: Cytokines and antigen-reactive antibodies

(2008) Elizabeth M. Bradshaw et al.   CLINICAL IMMUNOLOGY

Droplet microfluidics

(2008) Shia-Yen Teh et al.   LAB ON A CHIP

Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood

(2008) Rong Fan et al.   NATURE BIOTECHNOLOGY