Article
Multidisciplinary Sciences
Ruoxu Li, Ning Su, Xiaodong Ren, Xiange Sun, Wenman Li, Yuwei Li, Jin Li, Chen Chen, Hong Wang, Weiping Lu, Shaoli Deng, Qing Huang
Summary: This study developed a centrifugal microfluidics-based multiplex RT-RPA assay for rapid detection of SARS-CoV-2. The microfluidic chip could simultaneously detect three target genes and one reference gene, with high sensitivity and specificity. It has the potential to be used for point-of-care testing and detection of emerging SARS-CoV-2 variants.
Review
Medicine, Research & Experimental
Thai Pham, Ankush Tyagi, Yu-Sheng Wang, Jia Guo
Summary: Recent technological advances have enabled comprehensive protein profiling in single cells, providing new insights into various biological and medical fields.
WIRES MECHANISMS OF DISEASE
(2021)
Article
Chemistry, Multidisciplinary
Kai Guo, Hu Ye, Junhao Gu, Ye Tian
Summary: Absolute orientation estimation is a critical step in computer vision. This paper proposes a fast and simple method for absolute orientation estimation using a single vanishing point. The method utilizes the inertial measurement unit to simplify the estimation process and uses a non-linear optimization algorithm for solution refining. Experimental results demonstrate that the proposed method outperforms state-of-the-art solvers in terms of numerical stability, noise sensitivity, and computational speed in both synthetic data and real images.
APPLIED SCIENCES-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Juan Quintero-Blanco, Juan Jimenez, Andres Garzon
Summary: A simple and specific reverse transcription (RT) multiplex PCR assay was developed to directly diagnose the dsRNA totivirus genomes associated with the killer trait in S. cerevisiae yeast. The method accurately identified different variants of L-A and M totivirus and revealed unexpected associations in infected cells.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Duygu Beduk, Tutku Beduk, Josei Ilton de Oliveira Filho, Abdellatif Ait Lahcen, Ebru Aldemir, Emine Guler Celik, Khaled Nabil Salama, Suna Timur
Summary: Illicit drug use has become more widespread inrecent years and is associated with crime and public health issues. These drugs disrupt consciousness and affect perceptions and feelings. A laser-scribed graphene (LSG) sensing platform was developed for rapid and sensitive electrochemical detection of amphetamine (AMP), cocaine (COC), and benzodiazepine (BZD) simultaneously in saliva samples. The multiplex LSG sensor successfully detected target analytes in real saliva samples, providing a practical and affordable alternative to conventional drug-detection methods.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Wenwen Chen, Rongkai Cao, Wentao Su, Xu Zhang, Yuhai Xu, Peng Wang, Zhongqiao Gan, Yingying Xie, Hongjing Li, Jianhua Qin
Summary: The study proposes a simple, quick, and label-free method for isolating circulating exosomes from patient serum. Further validation through miRNA sequencing and RT-PCR reveals a potential new biomarker.
Article
Biochemical Research Methods
Dailin Gan, Jun Li
Summary: SCIBER is a new batch-effect-removal method that can remove batch effects before integrating multiple single-cell RNA-sequencing datasets to avoid misleading interpretations. It has better scalability and tunability compared to existing methods, and it can output expression data of individual genes for downstream analyses.
Article
Medicine, General & Internal
Hyunseul Jee, Minkyeong Choi, In Su Park, Junmin Lee, Woong Sik Jang, Chae Seung Lim
Summary: The study developed a rapid SARS-CoV-2 molecular diagnostic kit using rapid isothermal nucleic acid amplification technology and lateral flow assay, which can diagnose SARS-CoV-2 infection within 40 minutes with high sensitivity and specificity.
Article
Chemistry, Multidisciplinary
Dohwan Lee, Chia-Heng Chu, A. Fatih Sarioglu
Summary: This study presents a point-of-care toolkit for multiplex molecular diagnosis of SARS-CoV-2 and influenza A and B viruses in saliva samples. The assay can reliably detect the viruses at low concentrations visually from a colorimetric analysis. Autonomously performing a traditionally labor-intensive genetic assay on a disposable platform enables frequent, on-demand self-testing to track and contain current and future outbreaks.
Article
Biophysics
A. K. M. Arifuzzman, Norh Asmare, Tevhide Ozkaya-Ahmadov, Ozgun Civelekoglu, Ningquan Wang, A. Fatih Sarioglu
Summary: Characterization and identification of cell populations using surface markers are crucial in various applications. However, conventional techniques are limited by the need for immunolabeling, expensive instruments, and skilled operators. This study introduces an autonomous microchip that can electronically quantify the immunophenotypical composition of a cell suspension. The microchip captures different cell subtypes in separate microfluidic chambers and electrically monitors all on-chip activity, allowing real-time determination of subpopulation fractions. The capability to analyze immune cells without labels has the potential to enable remote testing and quality control in cell manufacturing.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Physical
Jie Yang, Zhijian Li, Rulin Bian, Zhaojie Su
Summary: The commercialization of Solid Oxide Fuel Cell (SOFC) systems is restricted by low reliability and durability issues, leading to a focus on fault diagnosis technology for extending system lifetime. This paper proposes an improved algorithm using single cell voltage as the only fault characteristic signal, achieving good recognition effects for diagnosing four representative system faults and their combination.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Chemistry, Analytical
Liwei Yang, Pratik Dutta, Ramana V. Davuluri, Jun Wang
Summary: A rapid and high-throughput method for detecting immunological disorders and distinguishing normal inflammation from sepsis using single-cell multiplex in situ tagging (scMIST) technology was presented. This approach allows simultaneous detection of 46 markers and cytokines from single cells without the need for special instruments. By analyzing T cell features and dynamics using scMIST, a machine learning model was developed and achieved 94% accuracy in predicting the group of mice.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Pei-Heng Lin, Bor-Ran Li
Summary: A microfluidic system with simple operation was developed in this study, providing reliable nucleic acid results from 18 uniform droplets controlled by micropipette regulation. The system demonstrated good applicability to genome detection and gene expression analysis.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Fengyun Li, Yong Zheng, Mei Yang, Yuewen Zhang, Qiaosheng Pu
Summary: This article introduces a portable testing device based on colorimetric detection on microchips with an array of micro through holes for increased optical length and an optical imaging system. The device can be used for reliable imaging with smartphones and can detect very low concentrations of biomarkers.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Infectious Diseases
Laetitia Velly, Marta Cancella de Abreu, David Boutolleau, Ilaria Cherubini, Enfel Houas, Alexandre Aurousseau, Pierre Hausfater
Summary: This study aimed to investigate whether coupling molecular syndromic respiratory panel (RP) testing with procalcitonin (PCT) measurement improves antibiotic stewardship in lower respiratory tract infection in the emergency department. The results showed that displaying PCT and real-time RP results to emergency physicians did not significantly reduce antibiotic exposure. However, even in the standard of care group, the duration and initiation rate of antibiotics were already low with routine PCT measurement.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Chemistry, Analytical
Jingxin Liu, Mohammed A. A. Abdullah, Liwei Yang, Jun Wang
ANALYTICAL CHEMISTRY
(2020)
Article
Biochemical Research Methods
Mohammed A. A. Abdullah, Nooshin Amini, Liwei Yang, Janet L. Paluh, Jun Wang
Article
Biochemistry & Molecular Biology
Mohammed A. A. Abdullah, Gamal El-Din A. A. Abuo-Rahma, El-Shimaa M. N. Abdelhafez, Heba A. Hassan, Rehab M. Abd El-Baky
BIOORGANIC CHEMISTRY
(2017)
Meeting Abstract
Chemistry, Multidisciplinary
Mohammed Ali, Gamal El-Din Abuo-Rahmaa, Rehab Abdelbaky, El Shimaa Abdel Hafez, Heba Hassan
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2016)