The importance of catalytic promiscuity for enzyme design and evolution

Enantioselective Aminohydroxylation of Styrenyl Olefins Catalyzed by an Engineered Hemoprotein

(2019) Inha Cho et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A “Broad Spectrum” Carbene Transferase for Synthesis of Chiral α-Trifluoromethylated Organoborons

(2019) Lur Alonso-Cotchico et al.   ACS Central Science

A Biocatalytic Platform for Synthesis of Chiral α-Trifluoromethylated Organoborons

(2019) Xiongyi Huang et al.   ACS Central Science

Asymmetric δ-Lactam Synthesis with a Monomeric Streptavidin Artificial Metalloenzyme

(2019) Isra S. Hassan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Understanding and Modulating Metalloenzymes with Unnatural Amino Acids, Non-Native Metal Ions, and Non-Native Metallocofactors

(2019) Evan N. Mirts et al.   ACCOUNTS OF CHEMICAL RESEARCH

Beyond the Second Coordination Sphere: Engineering Dirhodium Artificial Metalloenzymes To Enable Protein Control of Transition Metal Catalysis

(2019) Jared C. Lewis   ACCOUNTS OF CHEMICAL RESEARCH

LmrR: A Privileged Scaffold for Artificial Metalloenzymes

(2019) Gerard Roelfes   ACCOUNTS OF CHEMICAL RESEARCH

Directed Evolution of Artificial Metalloenzymes: A Universal Means to Tune the Selectivity of Transition Metal Catalysts?

(2019) Manfred T. Reetz   ACCOUNTS OF CHEMICAL RESEARCH

Photoenzymatic Catalysis Enables Radical-Mediated Ketone Reduction in Ene-Reductases

(2019) Braddock A. Sandoval et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Design and evolution of an enzyme with a non-canonical organocatalytic mechanism

(2019) Ashleigh J. Burke et al.   NATURE

Site-selective enzymatic C‒H amidation for synthesis of diverse lactams

(2019) Inha Cho et al.   SCIENCE

Chemical Optimization of Whole-Cell Transfer Hydrogenation Using Carbonic Anhydrase as Host Protein

(2019) Johannes G. Rebelein et al.   ACS Catalysis

The Effect of Cofactor Binding on the Conformational Plasticity of the Biological Receptors in Artificial Metalloenzymes: The Case Study of LmrR

(2019) Lur Alonso-Cotchico et al.   Frontiers in Chemistry

Optimization of the Turnover in Artificial Enzymes via Directed Evolution Results in the Coupling of Protein Dynamics to Chemistry

(2019) Joseph W. Schafer et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Photoexcitation of flavoenzymes enables a stereoselective radical cyclization

(2019) Kyle F. Biegasiewicz et al.   SCIENCE

Stereodivergent Evolution of Artificial Enzymes for the Michael Reaction

(2018) Xavier Garrabou et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

An Artificial Heme Enzyme for Cyclopropanation Reactions

(2018) Lara Villarino et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Reactivity and Selectivity of Iminium Organocatalysis Improved by a Protein Host

(2018) Alexander R Nödling et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Directed Evolution of Protein Catalysts

(2018) Cathleen Zeymer et al.   Annual Review of Biochemistry

Chemoselective Cyclopropanation over Carbene Y–H Insertion Catalyzed by an Engineered Carbene Transferase

(2018) Eric J. Moore et al.   JOURNAL OF ORGANIC CHEMISTRY

Catalytic promiscuity enabled by photoredox catalysis in nicotinamide-dependent oxidoreductases

(2018) Kyle F. Biegasiewicz et al.   Nature Chemistry

A designer enzyme for hydrazone and oxime formation featuring an unnatural catalytic aniline residue

(2018) Ivana Drienovská et al.   Nature Chemistry

Evolving artificial metalloenzymes via random mutagenesis

(2018) Hao Yang et al.   Nature Chemistry

Catalytic iron-carbene intermediate revealed in a cytochromeccarbene transferase

(2018) Russell D. Lewis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enzymatic construction of highly strained carbocycles

(2018) Kai Chen et al.   SCIENCE

E. coli surface display of streptavidin for directed evolution of an allylic deallylase

(2018) Tillmann Heinisch et al.   Chemical Science

Artificial Metalloenzyme Design with Unnatural Amino Acids and Non-Native Cofactors

(2018) Yang Yu et al.   ACS Catalysis

Stereoselective Enzymatic Synthesis of Heteroatom-Substituted Cyclopropanes

(2018) Oliver F. Brandenberg et al.   ACS Catalysis

Chimeric Streptavidins as Host Proteins for Artificial Metalloenzymes

(2018) Michela M. Pellizzoni et al.   ACS Catalysis

Diverse Engineered Heme Proteins Enable Stereodivergent Cyclopropanation of Unactivated Alkenes

(2018) Anders M. Knight et al.   ACS Central Science

A designed heme-[4Fe-4S] metalloenzyme catalyzes sulfite reduction like the native enzyme

(2018) Evan N. Mirts et al.   SCIENCE

Directed Evolution of a Designer Enzyme Featuring an Unnatural Catalytic Amino Acid

(2018) Clemens Mayer et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Alternate Heme Ligation Steers Activity and Selectivity in Engineered Cytochrome P450-Catalyzed Carbene-Transfer Reactions

(2018) Kai Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enzymatic assembly of carbon–carbon bonds via iron-catalysed sp3 C–H functionalization

(2018) Ruijie K. Zhang et al.   NATURE

Evolution of a highly active and enantiospecific metalloenzyme from short peptides

(2018) Sabine Studer et al.   SCIENCE

Biocatalytic hydrogen atom transfer: an invigorating approach to free-radical reactions

(2018) Yuji Nakano et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Manganese(V) Porphycene Complex Responsible for Inert C–H Bond Hydroxylation in a Myoglobin Matrix

(2017) Koji Oohora et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Importance of Scaffold Flexibility/Rigidity in the Design and Directed Evolution of Artificial Metallo-β-lactamases

(2017) Woon Ju Song et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemoselective, Enzymatic C–H Bond Amination Catalyzed by a Cytochrome P450 Containing an Ir(Me)-PIX Cofactor

(2017) Paweł Dydio et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Catalytic Cyclopropanation by Myoglobin Reconstituted with Iron Porphycene: Acceleration of Catalysis due to Rapid Formation of the Carbene Species

(2017) Koji Oohora et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enantioselective Hydrogen Atom Transfer: Discovery of Catalytic Promiscuity in Flavin-Dependent ‘Ene’-Reductases

(2017) Braddock A. Sandoval et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetically programmed chiral organoborane synthesis

(2017) S. B. Jennifer Kan et al.   NATURE

Enantioselective, intermolecular benzylic C–H amination catalysed by an engineered iron-haem enzyme

(2017) Christopher K. Prier et al.   Nature Chemistry

Multidrug resistance regulators (MDRs) as scaffolds for the design of artificial metalloenzymes

(2017) Manuela Bersellini et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

An algal photoenzyme converts fatty acids to hydrocarbons

(2017) Damien Sorigué et al.   SCIENCE

Design of an enantioselective artificial metallo-hydratase enzyme containing an unnatural metal-binding amino acid

(2017) Ivana Drienovská et al.   Chemical Science

Construction and in vivo assembly of a catalytically proficient and hyperthermostable de novo enzyme

(2017) Daniel W. Watkins et al.   Nature Communications

Artificial Metalloenzymes Based on the Biotin–Streptavidin Technology: Challenges and Opportunities

(2016) Tillmann Heinisch et al.   ACCOUNTS OF CHEMICAL RESEARCH

What are the Limitations of Enzymes in Synthetic Organic Chemistry?

(2016) Manfred T. Reetz   CHEMICAL RECORD

Enantiocomplementary Synthesis of γ-Nitroketones Using Designed and Evolved Carboligases

(2016) Xavier Garrabou et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Fast Knoevenagel Condensations Catalyzed by an Artificial Schiff-Base-Forming Enzyme

(2016) Xavier Garrabou et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Abiological catalysis by artificial haem proteins containing noble metals in place of iron

(2016) Hanna M. Key et al.   NATURE

Accessing non-natural reactivity by irradiating nicotinamide-dependent enzymes with light

(2016) Megan A. Emmanuel et al.   NATURE

Directed evolution of artificial metalloenzymes for in vivo metathesis

(2016) Markus Jeschek et al.   NATURE

Emergence of a catalytic tetrad during evolution of a highly active artificial aldolase

(2016) Richard Obexer et al.   Nature Chemistry

Installing hydrolytic activity into a completely de novo protein framework

(2016) Antony J. Burton et al.   Nature Chemistry

Library design and screening protocol for artificial metalloenzymes based on the biotin-streptavidin technology

(2016) Hendrik Mallin et al.   Nature Protocols

Enantioselective synthesis of an ophiobolin sesterterpene via a programmed radical cascade

(2016) Z. G. Brill et al.   SCIENCE

An artificial metalloenzyme with the kinetics of native enzymes

(2016) P. Dydio et al.   SCIENCE

Directed evolution of cytochrome c for carbon–silicon bond formation: Bringing silicon to life

(2016) S. B. Jennifer Kan et al.   SCIENCE

An enantioselective artificial Suzukiase based on the biotin–streptavidin technology

(2016) Anamitra Chatterjee et al.   Chemical Science

Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases

(2016) Jan-Ytzen van der Meer et al.   Nature Communications

Highly Stereoselective Biocatalytic Synthesis of Key Cyclopropane Intermediate to Ticagrelor

(2016) Kari E. Hernandez et al.   ACS Catalysis

A Promiscuous De Novo Retro-Aldolase Catalyzes Asymmetric Michael Additions via Schiff Base Intermediates

(2015) Xavier Garrabou et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Evidence for the Formation of an Enamine Species during Aldol and Michael-type Addition Reactions Promiscuously Catalyzed by 4-Oxalocrotonate Tautomerase

(2015) Harshwardhan Poddar et al.   CHEMBIOCHEM

Generation, Characterization, and Tunable Reactivity of Organometallic Fragments Bound to a Protein Ligand

(2015) Hanna M. Key et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Improving the Catalytic Performance of an Artificial Metalloenzyme by Computational Design

(2015) Tillmann Heinisch et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Supramolecular Assembly of Artificial Metalloenzymes Based on the Dimeric Protein LmrR as Promiscuous Scaffold

(2015) Jeffrey Bos et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids

(2015) Ivana Drienovská et al.   Chemical Science

Mysteries of Metals in Metalloenzymes

(2014) Crystal E. Valdez et al.   ACCOUNTS OF CHEMICAL RESEARCH

Improved Cyclopropanation Activity of Histidine-Ligated Cytochrome P450 Enables the Enantioselective Formal Synthesis of Levomilnacipran

(2014) Z. Jane Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Highly Diastereoselective and Enantioselective Olefin Cyclopropanation Using Engineered Myoglobin-Based Catalysts

(2014) Melanie Bordeaux et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Neutralizing the Detrimental Effect of Glutathione on Precious Metal Catalysts

(2014) Yvonne M. Wilson et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A designed supramolecular protein assembly with in vivo enzymatic activity

(2014) Woon Ju Song et al.   SCIENCE

P450-catalyzed asymmetric cyclopropanation of electron-deficient olefins under aerobic conditions

(2014) Hans Renata et al.   Catalysis Science & Technology

Dynamic multidrug recognition by multidrug transcriptional repressor LmrR

(2014) Koh Takeuchi et al.   Scientific Reports

Computational Enzyme Design

(2013) Gert Kiss et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Promiscuous Catalysis of Asymmetric Michael-Type Additions of Linear Aldehydes to β-Nitrostyrene by the Proline-Based Enzyme 4-Oxalocrotonate Tautomerase

(2013) Yufeng Miao et al.   CHEMBIOCHEM

Biocatalytic Michael-Type Additions of Acetaldehyde to Nitroolefins with the Proline-Based Enzyme 4-Oxalocrotonate Tautomerase Yielding Enantioenriched γ-Nitroaldehydes

(2013) Edzard M. Geertsema et al.   CHEMISTRY-A EUROPEAN JOURNAL

Radical SAM-mediated methylation reactions

(2013) Danica Galonić Fujimori   CURRENT OPINION IN CHEMICAL BIOLOGY

C(sp3)–H Bond Hydroxylation Catalyzed by Myoglobin Reconstituted with Manganese Porphycene

(2013) Koji Oohora et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template

(2013) Monika M. Meier et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Dual Anchoring Strategy for the Localization and Activation of Artificial Metalloenzymes Based on the Biotin–Streptavidin Technology

(2013) Jeremy M. Zimbron et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Precision is essential for efficient catalysis in an evolved Kemp eliminase

(2013) Rebecca Blomberg et al.   NATURE

A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo

(2013) Pedro S Coelho et al.   Nature Chemical Biology

Evolution of a designed retro-aldolase leads to complete active site remodeling

(2013) Lars Giger et al.   Nature Chemical Biology

Human carbonic anhydrase II as host protein for the creation of artificial metalloenzymes: the asymmetric transfer hydrogenation of imines

(2013) Fabien W. Monnard et al.   Chemical Science

Cytochrome P450-catalyzed insertion of carbenoids into N–H bonds

(2013) Z. Jane Wang et al.   Chemical Science

The crystal structure of multidrug-resistance regulator RamR with multiple drugs

(2013) Suguru Yamasaki et al.   Nature Communications

Reconstructing a Missing Link in the Evolution of a Recently Diverged Phosphotriesterase by Active-Site Loop Remodeling

(2012) Livnat Afriat-Jurnou et al.   BIOCHEMISTRY

Intramolecular Epistasis and the Evolution of a New Enzymatic Function

(2012) Sajid Noor et al.   PLoS One

Robust design and optimization of retroaldol enzymes

(2012) Eric A. Althoff et al.   PROTEIN SCIENCE

Iron-Catalyzed Cyclopropanation in 6 M KOH with in Situ Generation of Diazomethane

(2012) B. Morandi et al.   SCIENCE

Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes

(2012) P. S. Coelho et al.   SCIENCE

Biotinylated Rh(III) Complexes in Engineered Streptavidin for Accelerated Asymmetric C-H Activation

(2012) T. K. Hyster et al.   SCIENCE

Bridging between Organocatalysis and Biocatalysis: Asymmetric Addition of Acetaldehyde to β-Nitrostyrenes Catalyzed by a Promiscuous Proline-Based Tautomerase

(2011) Ellen Zandvoort et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Artificial Transfer Hydrogenases for the Enantioselective Reduction of Cyclic Imines

(2011) Marc Dürrenberger et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

De Novo Enzyme Design Using Rosetta3

(2011) Florian Richter et al.   PLoS One

Artificial Metalloenzymes Based on the Biotin−Avidin Technology: Enantioselective Catalysis and Beyond

(2010) Thomas R. Ward   ACCOUNTS OF CHEMICAL RESEARCH

Enzyme Promiscuity: A Mechanistic and Evolutionary Perspective

(2010) Olga Khersonsky and Dan S. Tawfik   Annual Review of Biochemistry

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Regioselective Hydroformylation of Styrene Using Rhodium-Substituted Carbonic Anhydrase

(2010) Qing Jing et al.   ChemCatChem

Artificial Metalloenzymes

(2010) Fiora Rosati et al.   ChemCatChem

Mutational effects and the evolution of new protein functions

(2010) Misha Soskine et al.   NATURE REVIEWS GENETICS

Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction

(2010) J. B. Siegel et al.   SCIENCE

A Novel Semi-biosynthetic Route for Artemisinin Production Using Engineered Substrate-Promiscuous P450BM3

(2009) Jeffrey A. Dietrich et al.   ACS Chemical Biology

Catalytic Improvement and Evolution of Atrazine Chlorohydrolase

(2009) C. Scott et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Hydrocarbon Hydroxylation by Cytochrome P450 Enzymes

(2009) Paul R. Ortiz de Montellano   CHEMICAL REVIEWS

Stereoselective Hydrogenation of Olefins Using Rhodium-Substituted Carbonic Anhydrase-A New Reductase

(2009) Qing Jing et al.   CHEMISTRY-A EUROPEAN JOURNAL

Design of functional metalloproteins

(2009) Yi Lu et al.   NATURE

Protein Dynamism and Evolvability

(2009) N. Tokuriki et al.   SCIENCE

Artificial Metalloenzymes for Asymmetric Allylic Alkylation on the Basis of the Biotin–Avidin Technology

(2008) Julien Pierron et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

X-Ray Structure and Designed Evolution of an Artificial Transfer Hydrogenase

(2008) Marc Creus et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Novel Type of Monoheme Cytochromec: Biochemical and Structural Characterization at 1.23 Å Resolution ofRhodothermus marinusCytochromec†‡

(2008) Meike Stelter et al.   BIOCHEMISTRY

Direct Epoxidation inCandida antarcticaLipase B Studied by Experiment and Theory

(2008) Maria Svedendahl et al.   CHEMBIOCHEM

Structure of the transcriptional regulator LmrR and its mechanism of multidrug recognition

(2008) Pramod Kumar Madoori et al.   EMBO JOURNAL

Intense Neutral Drifts Yield Robust and Evolvable Consensus Proteins

(2008) Shimon Bershtein et al.   JOURNAL OF MOLECULAR BIOLOGY

Kemp elimination catalysts by computational enzyme design

(2008) Daniela Röthlisberger et al.   NATURE

De Novo Computational Design of Retro-Aldol Enzymes

(2008) L. Jiang et al.   SCIENCE

Characterization of the Peroxidase Activity of CYP119, a Thermostable P450 FromSulfolobus acidocaldarius

(2007) Kersten S. Rabe et al.   CHEMBIOCHEM

Asymmetric catalytic cyclopropanation reactions in water

(2007) Irène Nicolas et al.   COORDINATION CHEMISTRY REVIEWS