Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 15, Issue 14, Pages 3069-3073Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ob00390k
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Funding
- European Research Council (ERC) [280010]
- Ministry of Education, Culture, and Science [024.001.035]
- European Research Council (ERC) [280010] Funding Source: European Research Council (ERC)
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The choice of protein scaffolds is an important element in the design of artificial metalloenzymes. Herein, we introduce Multidrug Resistance Regulators (MDRs) from the TetR family as a viable class of protein scaffolds for artificial metalloenzyme design. In vivo incorporation of the metal binding amino acid (2,2-bipyridin- 5yl) alanine (BpyA) by stop codon suppression methods was used to create artificial metalloenzymes from three members of the TetR family of MDRs: QacR, CgmR and RamR. Excellent results were achieved with QacR Y123BpyA in the Cu2+ catalyzed enantioselective vinylogous Friedel-Crafts alkylation reaction with ee's up to 94% of the opposite enantiomer that was achieved with other mutants and the previously reported LmrR-based artificial metalloenzymes.
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