Cholesterol accessibility at the ciliary membrane controls hedgehog signaling

Motional dynamics of single Patched1 molecules in cilia are controlled by Hedgehog and cholesterol

(2019) Lucien E. Weiss et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecular Discrimination between Two Conformations of Sphingomyelin in Plasma Membranes

(2019) Shreya Endapally et al.   CELL

Biochemical mechanisms of vertebrate hedgehog signaling

(2019) Jennifer H. Kong et al.   DEVELOPMENT

Establishing and regulating the composition of cilia for signal transduction

(2019) Maxence V. Nachury et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Cryo-EM structure of oxysterol-bound human Smoothened coupled to a heterotrimeric Gi

(2019) Xiaofeng Qi et al.   NATURE

Smoothened stimulation by membrane sterols drives Hedgehog pathway activity

(2019) Ishan Deshpande et al.   NATURE

Inhibition of tetrameric Patched1 by Sonic Hedgehog through an asymmetric paradigm

(2019) Hongwu Qian et al.   Nature Communications

Structures of vertebrate Patched and Smoothened reveal intimate links between cholesterol and Hedgehog signalling

(2019) Christiane Kowatsch et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Structural basis of sterol recognition by human hedgehog receptor PTCH1

(2019) Chao Qi et al.   Science Advances

Ostreolysin A and anthrolysin O use different mechanisms to control movement of cholesterol from the plasma membrane to the endoplasmic reticulum

(2019) Kristen A. Johnson et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Retrospective on Cholesterol Homeostasis: The Central Role of Scap

(2018) Michael S. Brown et al.   Annual Review of Biochemistry

An update on oxysterol biochemistry: New discoveries in lipidomics

(2018) William J. Griffiths et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Identification of 7α,24-dihydroxy-3-oxocholest-4-en-26-oic and 7α,25-dihydroxy-3-oxocholest-4-en-26-oic acids in human cerebrospinal fluid and plasma

(2018) Jonas Abdel-Khalik et al.   BIOCHIMIE

Multiple ligand binding sites regulate the Hedgehog signal transducer Smoothened in vertebrates

(2018) Eamon FX Byrne et al.   CURRENT OPINION IN CELL BIOLOGY

CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog

(2018) Ganesh V. Pusapati et al.   DEVELOPMENTAL CELL

Structural basis for the recognition of Sonic Hedgehog by human Patched1

(2018) Xin Gong et al.   SCIENCE

G protein–coupled receptors control the sensitivity of cells to the morphogen Sonic Hedgehog

(2018) Ganesh V. Pusapati et al.   Science Signaling

Transverse distribution of plasma membrane bilayer cholesterol: Picking sides

(2018) Theodore L. Steck et al.   TRAFFIC

G protein–coupled receptors control the sensitivity of cells to the morphogen Sonic Hedgehog

(2018) Ganesh V. Pusapati et al.   Science Signaling

Structural Basis of Smoothened Activation in Hedgehog Signaling

(2018) Pengxiang Huang et al.   CELL

Structures of human Patched and its complex with native palmitoylated sonic hedgehog

(2018) Xiaofeng Qi et al.   NATURE

Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex

(2018) Xiaofeng Qi et al.   SCIENCE

C24 Sphingolipids Govern the Transbilayer Asymmetry of Cholesterol and Lateral Organization of Model and Live-Cell Plasma Membranes

(2018) K.C. Courtney et al.   Cell Reports

Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched

(2018) Yunxiao Zhang et al.   CELL

Cilia-Associated Oxysterols Activate Smoothened

(2018) David R. Raleigh et al.   MOLECULAR CELL

Additional pathways of sterol metabolism: Evidence from analysis of Cyp27a1−/− mouse brain and plasma

(2018) William J. Griffiths et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

Untangling ciliary access and enrichment of two rhodopsin-like receptors using quantitative fluorescence microscopy reveals cell-specific sorting pathways

(2017) Ivayla I. Geneva et al.   MOLECULAR BIOLOGY OF THE CELL

Cholesterol Modification of Smoothened Is Required for Hedgehog Signaling

(2017) Xu Xiao et al.   MOLECULAR CELL

Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

(2017) Julia Joung et al.   Nature Protocols

Rapid, direct activity assays for Smoothened reveal Hedgehog pathway regulation by membrane cholesterol and extracellular sodium

(2017) Benjamin R. Myers et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Sonic Hedgehog Activates Phospholipase A2 to Enhance Smoothened Ciliary Translocation

(2017) Angela M. Arensdorf et al.   Cell Reports

Continuous transport of a small fraction of plasma membrane cholesterol to endoplasmic reticulum regulates total cellular cholesterol

(2017) Rodney Elwood Infante et al.   eLife

The importance of hydrogen bonding in sphingomyelin's membrane interactions with co-lipids

(2016) J. Peter Slotte   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Cellular Cholesterol Directly Activates Smoothened in Hedgehog Signaling

(2016) Pengxiang Huang et al.   CELL

Revisiting transbilayer distribution of lipids in the plasma membrane

(2016) Motohide Murate et al.   CHEMISTRY AND PHYSICS OF LIPIDS

Active membrane cholesterol as a physiological effector

(2016) Yvonne Lange et al.   CHEMISTRY AND PHYSICS OF LIPIDS

The Continuing Mystery of Lipid Rafts

(2016) Ilya Levental et al.   JOURNAL OF MOLECULAR BIOLOGY

Structural basis of Smoothened regulation by its extracellular domains

(2016) Eamon F. X. Byrne et al.   NATURE

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Endogenous B-ring oxysterols inhibit the Hedgehog component Smoothened in a manner distinct from cyclopamine or side-chain oxysterols

(2016) Navdar Sever et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Staurosporines decrease ORMDL proteins and enhance sphingomyelin synthesis resulting in depletion of plasmalemmal phosphatidylserine

(2016) Masashi Maekawa et al.   Scientific Reports

PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail

(2016) Kai Jiang et al.   PLOS BIOLOGY

Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling

(2016) Giovanni Luchetti et al.   eLife

Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling

(2016) Andres M Lebensohn et al.   eLife

Switch-like Responses of Two Cholesterol Sensors Do Not Require Protein Oligomerization in Membranes

(2015) Austin Gay et al.   BIOPHYSICAL JOURNAL

Modulation of Ciliary Phosphoinositide Content Regulates Trafficking and Sonic Hedgehog Signaling Output

(2015) Marcelo Chávez et al.   DEVELOPMENTAL CELL

Phosphoinositides Regulate Ciliary Protein Trafficking to Modulate Hedgehog Signaling

(2015) Francesc R. Garcia-Gonzalo et al.   DEVELOPMENTAL CELL

Reduced cholesterol levels impair Smoothened activation in Smith–Lemli–Opitz syndrome

(2015) Robert Blassberg et al.   HUMAN MOLECULAR GENETICS

Primary cilium suppression by SREBP1c involves distortion of vesicular trafficking by PLA2G3

(2015) Hannah Laura Gijs et al.   MOLECULAR BIOLOGY OF THE CELL

Regulation of the oncoprotein Smoothened by small molecules

(2015) Hayley J Sharpe et al.   Nature Chemical Biology

Endocannabinoids are conserved inhibitors of the Hedgehog pathway

(2015) Helena Khaliullina et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Disruption of Sphingolipid Biosynthesis Blocks Phagocytosis of Candida albicans

(2015) Fikadu G. Tafesse et al.   PLoS Pathogens

Identification of NPC1 as the target of U18666A, an inhibitor of lysosomal cholesterol export and Ebola infection

(2015) Feiran Lu et al.   eLife

Improved vectors and genome-wide libraries for CRISPR screening

(2014) Neville E Sanjana et al.   NATURE METHODS

How do cilia organize signalling cascades?

(2014) M. V. Nachury   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Tracking Cholesterol/Sphingomyelin-Rich Membrane Domains with the Ostreolysin A-mCherry Protein

(2014) Matej Skočaj et al.   PLoS One

Three pools of plasma membrane cholesterol and their relation to cholesterol homeostasis

(2014) Akash Das et al.   eLife

Stability and Stoichiometry of Bilayer Phospholipid–Cholesterol Complexes: Relationship to Cellular Sterol Distribution and Homeostasis

(2013) Yvonne Lange et al.   BIOCHEMISTRY

The Ciliary G-Protein-Coupled Receptor Gpr161 Negatively Regulates the Sonic Hedgehog Pathway via cAMP Signaling

(2013) Saikat Mukhopadhyay et al.   CELL

Controlling Cholesterol Synthesis beyond 3-Hydroxy-3-methylglutaryl-CoA Reductase (HMGCR)

(2013) Laura J. Sharpe et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

An in vitro assay for entry into cilia reveals unique properties of the soluble diffusion barrier

(2013) David K. Breslow et al.   JOURNAL OF CELL BIOLOGY

Activation of Hedgehog signaling by loss of GNAS causes heterotopic ossification

(2013) Jean B Regard et al.   NATURE MEDICINE

Use of mutant 125I-Perfringolysin O to probe transport and organization of cholesterol in membranes of animal cells

(2013) A. Das et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A comprehensive method for extraction and quantitative analysis of sterols and secosteroids from human plasma

(2012) Jeffrey G. McDonald et al.   JOURNAL OF LIPID RESEARCH

Oxysterols are allosteric activators of the oncoprotein Smoothened

(2012) Sigrid Nachtergaele et al.   Nature Chemical Biology

Defects in cholesterol synthesis genes in mouse and in humans: lessons for drug development and safer treatments

(2011) Simon Horvat et al.   DRUG METABOLISM REVIEWS

The Hedgehog Receptor Patched Is Involved in Cholesterol Transport

(2011) Michel Bidet et al.   PLoS One

Survival strategies of a sterol auxotroph

(2010) M. Carvalho et al.   DEVELOPMENT

Accessibility of Cholesterol in Endoplasmic Reticulum Membranes and Activation of SREBP-2 Switch Abruptly at a Common Cholesterol Threshold

(2010) Anna Sokolov et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Malformation syndromes caused by disorders of cholesterol synthesis

(2010) Forbes D. Porter et al.   JOURNAL OF LIPID RESEARCH

Functional genomic screen for modulators of ciliogenesis and cilium length

(2010) Joon Kim et al.   NATURE

Cell cholesterol homeostasis: Mediation by active cholesterol

(2010) Theodore L. Steck et al.   TRENDS IN CELL BIOLOGY

Cholesterol Exposure at the Membrane Surface Is Necessary and Sufficient to Trigger Perfringolysin O Binding

(2009) John J. Flanagan et al.   BIOCHEMISTRY

Nuclear Receptor DHR96 Acts as a Sentinel for Low Cholesterol Concentrations in Drosophila melanogaster

(2009) M. Bujold et al.   MOLECULAR AND CELLULAR BIOLOGY

Structural insights into hedgehog ligand sequestration by the human hedgehog-interacting protein HHIP

(2009) Benjamin Bishop et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Hedgehog signal transduction by Smoothened: Pharmacologic evidence for a 2-step activation process

(2009) R. Rohatgi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Switch-like Control of SREBP-2 Transport Triggered by Small Changes in ER Cholesterol: A Delicate Balance

(2008) Arun Radhakrishnan et al.   Cell Metabolism

Intraflagellar transport, cilia, and mammalian Hedgehog signaling: Analysis in mouse embryonic fibroblasts

(2008) Polloneal Jymmiel R. Ocbina et al.   DEVELOPMENTAL DYNAMICS

Cholesterol feedback: from Schoenheimer's bottle to Scap's MELADL

(2008) Michael S. Brown et al.   JOURNAL OF LIPID RESEARCH

Preservation of Genes Involved in Sterol Metabolism in Cholesterol Auxotrophs: Facts and Hypotheses

(2008) Giovanna Vinci et al.   PLoS One