Multiple roles of DNA2 nuclease/helicase in DNA metabolism, genome stability and human diseases

Regulatory control of Sgs1 and Dna2 during eukaryotic DNA end resection

(2019) Chaoyou Xue et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RPA Phosphorylation Inhibits DNA Resection

(2019) Michael M. Soniat et al.   MOLECULAR CELL

Human RIF1-Protein Phosphatase 1 Prevents Degradation and Breakage of Nascent DNA on Replication Stalling

(2019) Javier Garzón et al.   Cell Reports

TRAF6 mediates human DNA2 polyubiquitination and nuclear localization to maintain nuclear genome integrity

(2019) Yuan Meng et al.   NUCLEIC ACIDS RESEARCH

RIF1 promotes replication fork protection and efficient restart to maintain genome stability

(2019) Chirantani Mukherjee et al.   Nature Communications

Novel mutations in DNA2 associated with myopathy and mtDNA instability

(2019) Dario Ronchi et al.   Annals of Clinical and Translational Neurology

Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe

(2018) Pol Margalef et al.   CELL

hDNA2 nuclease/helicase promotes centromeric DNA replication and genome stability

(2018) Zhengke Li et al.   EMBO JOURNAL

Histone Methylation by SETD1A Protects Nascent DNA through the Nucleosome Chaperone Activity of FANCD2

(2018) Martin R. Higgs et al.   MOLECULAR CELL

The Importance of Poly(ADP-Ribose) Polymerase as a Sensor of Unligated Okazaki Fragments during DNA Replication

(2018) Hana Hanzlikova et al.   MOLECULAR CELL

K63-linked polyubiquitin chains bind to DNA to facilitate DNA damage repair

(2018) Pengda Liu et al.   Science Signaling

K63-linked polyubiquitin chains bind to DNA to facilitate DNA damage repair

(2018) Pengda Liu et al.   Science Signaling

Affinity for DNA Contributes to NLS Independent Nuclear Localization of MeCP2

(2018) Matthew J. Lyst et al.   Cell Reports

Dna2 nuclease deficiency results in large and complex DNA insertions at chromosomal breaks

(2018) Yang Yu et al.   NATURE

Processing of eukaryotic Okazaki fragments by redundant nucleases can be uncoupled from ongoing DNA replication in vivo

(2018) Malik Kahli et al.   NUCLEIC ACIDS RESEARCH

Dna2 processes behind the fork long ssDNA flaps generated by Pif1 and replication-dependent strand displacement

(2018) Silvia Emma Rossi et al.   Nature Communications

Analysis of centrosome and DNA damage response in PLK4 associated Seckel syndrome

(2017) Tuba Dinçer et al.   EUROPEAN JOURNAL OF HUMAN GENETICS

A novel role of the Dna2 translocase function in DNA break resection

(2017) Adam S. Miller et al.   GENES & DEVELOPMENT

The motor activity of DNA2 functions as an ssDNA translocase to promote DNA end resection

(2017) Maryna Levikova et al.   GENES & DEVELOPMENT

Abro1 maintains genome stability and limits replication stress by protecting replication fork stability

(2017) Shengfeng Xu et al.   GENES & DEVELOPMENT

Direct Visualization of RNA-DNA Primer Removal from Okazaki Fragments Provides Support for Flap Cleavage and Exonucleolytic Pathways in Eukaryotic Cells

(2017) Bochao Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Smarcal1-Mediated Fork Reversal Triggers Mre11-Dependent Degradation of Nascent DNA in the Absence of Brca2 and Stable Rad51 Nucleofilaments

(2017) Arun Mouli Kolinjivadi et al.   MOLECULAR CELL

Novel truncating variant in DNA2 -related congenital onset myopathy and ptosis suggests genotype–phenotype correlation

(2017) P. Phowthongkum et al.   NEUROMUSCULAR DISORDERS

Dynamic DNA binding, junction recognition and G4 melting activity underlie the telomeric and genome-wide roles of human CST

(2017) Anukana Bhattacharjee et al.   NUCLEIC ACIDS RESEARCH

Oxidative DNA damage is epigenetic by regulating gene transcription via base excision repair

(2017) Aaron M. Fleming et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Role of the Pif1-PCNA Complex in Pol δ-Dependent Strand Displacement DNA Synthesis and Break-Induced Replication

(2017) Olga Buzovetsky et al.   Cell Reports

Inhibition of DNA2 nuclease as a therapeutic strategy targeting replication stress in cancer cells

(2017) S Kumar et al.   Oncogenesis

A Comparative Study of G-Quadruplex Unfolding and DNA Reeling Activities of Human RECQ5 Helicase

(2016) Jagat B. Budhathoki et al.   BIOPHYSICAL JOURNAL

Human mitochondrial DNA replication machinery and disease

(2016) Matthew J Young et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

The Werner syndrome RECQ helicase targets G4 DNA in human cells to modulate transcription

(2016) Weiliang Tang et al.   HUMAN MOLECULAR GENETICS

5′ to 3′ Unfolding Directionality of DNA Secondary Structures by Replication Protein A

(2016) Layal Safa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase

(2016) Fabrizio Villa et al.   MOLECULAR CELL

Centromeric DNA replication reconstitution reveals DNA loops and ATR checkpoint suppression

(2016) Antoine Aze et al.   NATURE CELL BIOLOGY

Nucleolytic processing of aberrant replication intermediates by an Exo1-Dna2-Sae2 axis counteracts fork collapse-driven chromosome instability

(2016) Arianna Colosio et al.   NUCLEIC ACIDS RESEARCH

Enrichment of Cdk1-cyclins at DNA double-strand breaks stimulates Fun30 phosphorylation and DNA end resection

(2016) Xuefeng Chen et al.   NUCLEIC ACIDS RESEARCH

G-quadruplexes and helicases

(2016) Oscar Mendoza et al.   NUCLEIC ACIDS RESEARCH

Repair Pathway Choices and Consequences at the Double-Strand Break

(2016) Raphael Ceccaldi et al.   TRENDS IN CELL BIOLOGY

A Selective Small Molecule DNA2 Inhibitor for Sensitization of Human Cancer Cells to Chemotherapy

(2016) Wenpeng Liu et al.   EBioMedicine

Primer removal during mammalian mitochondrial DNA replication

(2015) Jay P. Uhler et al.   DNA REPAIR

MERIT40 cooperates with BRCA2 to resolve DNA interstrand cross-links

(2015) Qinqin Jiang et al.   GENES & DEVELOPMENT

DNA2 drives processing and restart of reversed replication forks in human cells

(2015) Saravanabhavan Thangavel et al.   JOURNAL OF CELL BIOLOGY

Rad51-mediated replication fork reversal is a global response to genotoxic treatments in human cells

(2015) Ralph Zellweger et al.   JOURNAL OF CELL BIOLOGY

BOD1L Is Required to Suppress Deleterious Resection of Stressed Replication Forks

(2015) Martin R. Higgs et al.   MOLECULAR CELL

A Dominant Mutation in Human RAD51 Reveals Its Function in DNA Interstrand Crosslink Repair Independent of Homologous Recombination

(2015) Anderson T. Wang et al.   MOLECULAR CELL

TheSaccharomyces cerevisiaeDna2 can function as a sole nuclease in the processing of Okazaki fragments in DNA replication

(2015) Maryna Levikova et al.   NUCLEIC ACIDS RESEARCH

BRCA1 and CtIP Are Both Required to Recruit Dna2 at Double-Strand Breaks in Homologous Recombination

(2015) Nguyen Ngoc Hoa et al.   PLoS One

Homologous Recombination Deficiency: Exploiting the Fundamental Vulnerability of Ovarian Cancer

(2015) P. A. Konstantinopoulos et al.   Cancer Discovery

Dna2 nuclease-helicase structure, mechanism and regulation by Rpa

(2015) Chun Zhou et al.   eLife

The DNA2 nuclease/helicase is an estrogen-dependent gene mutated in breast and ovarian cancers

(2015) Carmit Strauss et al.   Oncotarget

Preventing over-resection by DNA2 helicase/nuclease suppresses repair defects in Fanconi anemia cells

(2014) Kenneth K Karanja et al.   CELL CYCLE

Genomic analysis of primordial dwarfism reveals novel disease genes

(2014) R. Shaheen et al.   GENOME RESEARCH

DNA2 Cooperates with the WRN and BLM RecQ Helicases to Mediate Long-range DNA End Resection in Human Cells

(2014) Andreas Sturzenegger et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The 9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1

(2014) Greg H.P. Ngo et al.   NUCLEIC ACIDS RESEARCH

Mutations in DNA2 Link Progressive Myopathy to Mitochondrial DNA Instability

(2013) Dario Ronchi et al.   AMERICAN JOURNAL OF HUMAN GENETICS

Mammalian DNA2 helicase/nuclease cleaves G-quadruplex DNA and is required for telomere integrity

(2013) Weiqiang Lin et al.   EMBO JOURNAL

Lagging strand maturation factor Dna2 is a component of the replication checkpoint initiation machinery

(2013) S. Kumar et al.   GENES & DEVELOPMENT

Dna2 Is Involved in CA Strand Resection and Nascent Lagging Strand Completion at Native Yeast Telomeres

(2013) Martin E. Budd et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Pif1 helicase and Polδ promote recombination-coupled DNA synthesis via bubble migration

(2013) Marenda A. Wilson et al.   NATURE

Pif1 family helicases suppress genome instability at G-quadruplex motifs

(2013) Katrin Paeschke et al.   NATURE

Quantitative visualization of DNA G-quadruplex structures in human cells

(2013) Giulia Biffi et al.   Nature Chemistry

Nuclease activity of Saccharomyces cerevisiae Dna2 inhibits its potent DNA helicase activity

(2013) M. Levikova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Repair of Strand Breaks by Homologous Recombination

(2013) M. Jasin et al.   Cold Spring Harbor Perspectives in Biology

Rescuing Stalled or Damaged Replication Forks

(2013) J. T. P. Yeeles et al.   Cold Spring Harbor Perspectives in Biology

Substrate-Selective Repair and Restart of Replication Forks by DNA Translocases

(2013) Rémy Bétous et al.   Cell Reports

Human Nuclease/Helicase DNA2 Alleviates Replication Stress by Promoting DNA End Resection

(2012) G. Peng et al.   CANCER RESEARCH

The Intra-S Phase Checkpoint Targets Dna2 to Prevent Stalled Replication Forks from Reversing

(2012) Jiazhi Hu et al.   CELL

RTEL1 Dismantles T Loops and Counteracts Telomeric G4-DNA to Maintain Telomere Integrity

(2012) Jean-Baptiste Vannier et al.   CELL

Dna2 Offers Support for Stalled Forks

(2012) Mong Sing Lai et al.   CELL

Telomeric 3′ Overhangs Derive from Resection by Exo1 and Apollo and Fill-In by POT1b-Associated CST

(2012) Peng Wu et al.   CELL

DNA2 and EXO1 in replication-coupled, homology-directed repair and in the interplay between HDR and the FA/BRCA network

(2012) Kenneth K. Karanja et al.   CELL CYCLE

Okazaki Fragment Processing-independent Role for Human Dna2 Enzyme during DNA Replication

(2012) Julien P. Duxin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Refined Preparation and Use of Anti-diglycine Remnant (K-ε-GG) Antibody Enables Routine Quantification of 10,000s of Ubiquitination Sites in Single Proteomics Experiments

(2012) Namrata D. Udeshi et al.   MOLECULAR & CELLULAR PROTEOMICS

The yeast Fun30 and human SMARCAD1 chromatin remodellers promote DNA end resection

(2012) Thomas Costelloe et al.   NATURE

The Fun30 nucleosome remodeller promotes resection of DNA double-strand break ends

(2012) Xuefeng Chen et al.   NATURE

Cross talk between the nuclease and helicase activities of Dna2: role of an essential iron–sulfur cluster domain

(2012) Subhash Pokharel et al.   NUCLEIC ACIDS RESEARCH

MMS19 Assembles Iron-Sulfur Proteins Required for DNA Metabolism and Genomic Integrity

(2012) O. Stehling et al.   SCIENCE

The cBio Cancer Genomics Portal: An Open Platform for Exploring Multidimensional Cancer Genomics Data: Figure 1.

(2012) Ethan Cerami et al.   Cancer Discovery

Hallmarks of Cancer: The Next Generation

(2011) Douglas Hanahan et al.   CELL

Inviability of aDNA2deletion mutant is due to the DNA damage checkpoint

(2011) Martin E. Budd et al.   CELL CYCLE

BLM-DNA2-RPA-MRN and EXO1-BLM-RPA-MRN constitute two DNA end resection machineries for human DNA break repair

(2011) A. V. Nimonkar et al.   GENES & DEVELOPMENT

Characterization of the Endonuclease and ATP-dependent Flap Endo/Exonuclease of Dna2

(2011) Barbara K. Fortini et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cell cycle regulation of DNA double-strand break end resection by Cdk1-dependent Dna2 phosphorylation

(2011) Xuefeng Chen et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Mechanistic analysis of Xenopus EXO1's function in 5'-strand resection at DNA double-strand breaks

(2011) S. Liao et al.   NUCLEIC ACIDS RESEARCH

Okazaki fragment maturation: nucleases take centre stage

(2011) L. Zheng et al.   Journal of Molecular Cell Biology

Xenopus DNA2 is a helicase/nuclease that is found in complexes with replication proteins And-1/Ctf4 and Mcm10 and DSB response proteins Nbs1 and ATM

(2010) Karen E. Wawrousek et al.   CELL CYCLE

DNA end resection by CtIP and exonuclease 1 prevents genomic instability

(2010) Wassim Eid et al.   EMBO REPORTS

Dna2 Exhibits a Unique Strand End-dependent Helicase Function

(2010) Lata Balakrishnan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Components of the Secondary Pathway Stimulate the Primary Pathway of Eukaryotic Okazaki Fragment Processing

(2010) Ryan A. Henry et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Mutation and Expression ofDNA2Gene in Gastric and Colorectal Carcinomas

(2010) Sung Hak Lee et al.   Korean Journal of Pathology

Mechanism of the ATP-dependent DNA end-resection machinery from Saccharomyces cerevisiae

(2010) Hengyao Niu et al.   NATURE

DNA end resection by Dna2–Sgs1–RPA and its stimulation by Top3–Rmi1 and Mre11–Rad50–Xrs2

(2010) Petr Cejka et al.   NATURE

Maintaining genome stability at the replication fork

(2010) Dana Branzei et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Current challenges for the early clinical development of anticancer drugs in the era of molecularly targeted agents

(2010) Christophe Le Tourneau et al.   Targeted Oncology

Mammalian Telomeres Resemble Fragile Sites and Require TRF1 for Efficient Replication

(2009) Agnel Sfeir et al.   CELL

Mechanisms and Signals for the Nuclear Import of Proteins

(2009) Natalia Freitas et al.   CURRENT GENOMICS

Pif1 Helicase Lengthens Some Okazaki Fragment Flaps Necessitating Dna2 Nuclease/Helicase Action in the Two-nuclease Processing Pathway

(2009) Jason E. Pike et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Acetylation of Dna2 Endonuclease/Helicase and Flap Endonuclease 1 by p300 Promotes DNA Stability by Creating Long Flap Intermediates

(2009) Lata Balakrishnan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Human Dna2 Is a Nuclear and Mitochondrial DNA Maintenance Protein

(2009) J. P. Duxin et al.   MOLECULAR AND CELLULAR BIOLOGY

Multiple Pathways Regulate 3′ Overhang Generation at S. cerevisiae Telomeres

(2009) Diego Bonetti et al.   MOLECULAR CELL

Dna2 is a structure-specific nuclease, with affinity for 5′-flap intermediates

(2009) Jason A. Stewart et al.   NUCLEIC ACIDS RESEARCH

Stabilization of quadruplex DNA perturbs telomere replication leading to the activation of an ATR-dependent ATM signaling pathway

(2009) Angela Rizzo et al.   NUCLEIC ACIDS RESEARCH

Interplay of Mre11 Nuclease with Dna2 plus Sgs1 in Rad51-Dependent Recombinational Repair

(2009) Martin E. Budd et al.   PLoS One

Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifs

(2009) S. Kosugi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

How Telomeres Solve the End-Protection Problem

(2009) T. de Lange   SCIENCE

Sgs1 Helicase and Two Nucleases Dna2 and Exo1 Resect DNA Double-Strand Break Ends

(2008) Zhu Zhu et al.   CELL

The endless tale of non-homologous end-joining

(2008) Eric Weterings et al.   CELL RESEARCH

Mitochondrial base excision repair of uracil and AP sites takes place by single-nucleotide insertion and long-patch DNA synthesis

(2008) Mansour Akbari et al.   DNA REPAIR

DNA helicases Sgs1 and BLM promote DNA double-strand break resection

(2008) S. Gravel et al.   GENES & DEVELOPMENT

Processing of G4 DNA by Dna2 Helicase/Nuclease and Replication Protein A (RPA) Provides Insights into the Mechanism of Dna2/RPA Substrate Recognition

(2008) Taro Masuda-Sasa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Dynamic Removal of Replication Protein A by Dna2 Facilitates Primer Cleavage during Okazaki Fragment Processing inSaccharomyces cerevisiae

(2008) Jason A. Stewart et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Pif1 Helicase Directs Eukaryotic Okazaki Fragments toward the Two-nuclease Cleavage Pathway for Primer Removal

(2008) Marie L. Rossi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Flexibility of Eukaryotic Okazaki Fragment Maturation through Regulated Strand Displacement Synthesis

(2008) Carrie M. Stith et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

G4 Resolvase 1 Binds Both DNA and RNA Tetramolecular Quadruplex with High Affinity and Is the Major Source of Tetramolecular Quadruplex G4-DNA and G4-RNA Resolving Activity in HeLa Cell Lysates

(2008) Steven D. Creacy et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

FANCJ Helicase Defective in Fanconia Anemia and Breast Cancer Unwinds G-Quadruplex DNA To Defend Genomic Stability

(2008) Y. Wu et al.   MOLECULAR AND CELLULAR BIOLOGY

Removal of Oxidative DNA Damage via FEN1-Dependent Long-Patch Base Excision Repair in Human Cell Mitochondria

(2008) P. Liu et al.   MOLECULAR AND CELLULAR BIOLOGY

Division of Labor at the Eukaryotic Replication Fork

(2008) Stephanie A. Nick McElhinny et al.   MOLECULAR CELL

Human DNA2 Is a Mitochondrial Nuclease/Helicase for Efficient Processing of DNA Replication and Repair Intermediates

(2008) Li Zheng et al.   MOLECULAR CELL

Sae2, Exo1 and Sgs1 collaborate in DNA double-strand break processing

(2008) Eleni P. Mimitou et al.   NATURE

Identification of the Xenopus DNA2 protein as a major nuclease for the 5'->3' strand-specific processing of DNA ends

(2008) S. Liao et al.   NUCLEIC ACIDS RESEARCH