Emerging concepts in pseudoenzyme classification, evolution, and signaling

Phylogenetic Distribution and Diversity of Bacterial Pseudo-Orthocaspases Underline Their Putative Role in Photosynthesis

(2019) Marina Klemenčič et al.   Frontiers in Plant Science

A poxvirus pseudokinase represses viral DNA replication via a pathway antagonized by its paralog kinase

(2019) Annabel T. Olson et al.   PLoS Pathogens

The pseudokinase domains of guanylyl cyclase–A and –B allosterically increase the affinity of their catalytic domains for substrate

(2019) Aaron B. Edmund et al.   Science Signaling

Divergent kinase regulates membrane ultrastructure of theToxoplasmaparasitophorous vacuole

(2019) Tsebaot Beraki et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Wheat tandem kinases provide insights on disease-resistance gene flow and host-parasite co-evolution

(2019) Valentina Klymiuk et al.   PLANT JOURNAL

Prospects for pharmacological targeting of pseudokinases

(2019) Jennifer E. Kung et al.   NATURE REVIEWS DRUG DISCOVERY

The pseudokinase TRIB1 toggles an intramolecular switch to regulate COP1 nuclear export

(2019) Jennifer E Kung et al.   EMBO JOURNAL

The demise of catalysis, but new functions arise: pseudoenzymes as the phoenixes of the protein world

(2019) Constance J. Jeffery   BIOCHEMICAL SOCIETY TRANSACTIONS

Metabolic control of BRISC–SHMT2 assembly regulates immune signalling

(2019) Miriam Walden et al.   NATURE

Structures of ligand-occupied β-Klotho complexes reveal a molecular mechanism underlying endocrine FGF specificity and activity

(2019) Ekaterina S. Kuzina et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Human pantothenate kinase 4 is a pseudo‐pantothenate kinase

(2019) Jiangwei Yao et al.   PROTEIN SCIENCE

Reconstitution and structure of a plant NLR resistosome conferring immunity

(2019) Jizong Wang et al.   SCIENCE

Bacterial pseudokinase catalyzes protein polyglutamylation to inhibit the SidE-family ubiquitin ligases

(2019) Miles H. Black et al.   SCIENCE

Ligand-triggered allosteric ADP release primes a plant NLR complex

(2019) Jizong Wang et al.   SCIENCE

Tracing the origin and evolution of pseudokinases across the tree of life

(2019) Annie Kwon et al.   Science Signaling

FAM105A/OTULINL Is a Pseudodeubiquitinase of the OTU-Class that Localizes to the ER Membrane

(2019) Derek F. Ceccarelli et al.   STRUCTURE

BubR1 phosphorylates CENP-E as a switch enabling the transition from lateral association to end-on capture of spindle microtubules

(2019) Yuejia Huang et al.   CELL RESEARCH

Pseudo-DUBs as allosteric activators and molecular scaffolds of protein complexes

(2018) Miriam Walden et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

The FAM83 family of proteins: from pseudo-PLDs to anchors for CK1 isoforms

(2018) Polyxeni Bozatzi et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Evolution of chalcone isomerase from a noncatalytic ancestor

(2018) Miriam Kaltenbach et al.   Nature Chemical Biology

Evolution of cyclohexadienyl dehydratase from an ancestral solute-binding protein

(2018) Ben E. Clifton et al.   Nature Chemical Biology

HMMER web server: 2018 update

(2018) Simon C Potter et al.   NUCLEIC ACIDS RESEARCH

The DUF1669 domain of FAM83 family proteins anchor casein kinase 1 isoforms

(2018) Luke J. Fulcher et al.   Science Signaling

Dimerization of the Pragmin Pseudo-Kinase Regulates Protein Tyrosine Phosphorylation

(2018) Céline Lecointre et al.   STRUCTURE

Structure and evolution of the Fam20 kinases

(2018) Hui Zhang et al.   Nature Communications

The DUF1669 domain of FAM83 family proteins anchor casein kinase 1 isoforms

(2018) Luke J. Fulcher et al.   Science Signaling

Protein AMPylation by an Evolutionarily Conserved Pseudokinase

(2018) Anju Sreelatha et al.   CELL

Nature of Long-Range Evolutionary Constraint in Enzymes: Insights from Comparison to Pseudoenzymes with Similar Structures

(2018) Avital Sharir-Ivry et al.   MOLECULAR BIOLOGY AND EVOLUTION

Substrate binding allosterically relieves autoinhibition of the pseudokinase TRIB1

(2018) Sam A. Jamieson et al.   Science Signaling

Covalent inhibitors of EGFR family protein kinases induce degradation of human Tribbles 2 (TRIB2) pseudokinase in cancer cells

(2018) Daniel M. Foulkes et al.   Science Signaling

Trypanosomatid Deoxyhypusine Synthase Activity Is Dependent on Shared Active-Site Complementation between Pseudoenzyme Paralogs

(2018) Gustavo A. Afanador et al.   STRUCTURE

The N-Terminal GTPase Domain of p190RhoGAP Proteins Is a PseudoGTPase

(2018) Amy L. Stiegler et al.   STRUCTURE

PseudoGTPase domains in p190RhoGAP proteins: a mini-review

(2018) Amy L. Stiegler et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Structure of Plasmodium falciparum Rh5–CyRPA–Ripr invasion complex

(2018) Wilson Wong et al.   NATURE

UniProt: a worldwide hub of protein knowledge

(2018)   NUCLEIC ACIDS RESEARCH

CATH: expanding the horizons of structure-based functional annotations for genome sequences

(2018) Ian Sillitoe et al.   NUCLEIC ACIDS RESEARCH

Capturing variation impact on molecular interactions in the IMEx Consortium mutations data set

(2018) N. del-Toro et al.   Nature Communications

Pseudokinases: update on their functions and evaluation as new drug targets

(2017) Dominic P Byrne et al.   Future Medicinal Chemistry

The crystal structure of pseudokinase PEAK1 (Sugen kinase 269) reveals an unusual catalytic cleft and a novel mode of kinase fold dimerization

(2017) Byung Hak Ha et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cryo-EM structure of a human spliceosome activated for step 2 of splicing

(2017) Karl Bertram et al.   NATURE

Gene3D: Extensive prediction of globular domains in proteins

(2017) Tony E Lewis et al.   NUCLEIC ACIDS RESEARCH

Mechanism and Catalytic Site Atlas (M-CSA): a database of enzyme reaction mechanisms and active sites

(2017) António J M Ribeiro et al.   NUCLEIC ACIDS RESEARCH

Genomics and evolution of protein phosphatases

(2017) Mark J. Chen et al.   Science Signaling

Tribbles in the 21st Century: The Evolving Roles of Tribbles Pseudokinases in Biology and Disease

(2017) Patrick A. Eyers et al.   TRENDS IN CELL BIOLOGY

Structure of SgK223 pseudokinase reveals novel mechanisms of homotypic and heterotypic association

(2017) Onisha Patel et al.   Nature Communications

Tsg101 chaperone function revealed by HIV-1 assembly inhibitors

(2017) Madeleine Strickland et al.   Nature Communications

Structure of Fam20A reveals a pseudokinase featuring a unique disulfide pattern and inverted ATP-binding

(2017) Jixin Cui et al.   eLife

Genomics and evolution of protein phosphatases

(2017) Mark J. Chen et al.   Science Signaling

The evolving world of pseudoenzymes: proteins, prejudice and zombies

(2016) Patrick A. Eyers et al.   BMC BIOLOGY

The pseudophosphatase STYX targets the F‐box of FBXW7 and inhibits SCF FBXW7 function

(2016) Veronika Reiterer et al.   EMBO JOURNAL

The role of mouse 2′,5′-oligoadenylate synthetase 1 paralogs

(2016) Enas Elkhateeb et al.   INFECTION GENETICS AND EVOLUTION

Homo- and Heterotypic Association Regulates Signaling by the SgK269/PEAK1 and SgK223 Pseudokinases

(2016) Ling Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Large-Scale Analysis Exploring Evolution of Catalytic Machineries and Mechanisms in Enzyme Superfamilies

(2016) Nicholas Furnham et al.   JOURNAL OF MOLECULAR BIOLOGY

Cerebellar Ataxia and Coenzyme Q Deficiency through Loss of Unorthodox Kinase Activity

(2016) Jonathan A. Stefely et al.   MOLECULAR CELL

The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease

(2016) Daniela Lazzaretti et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

UniProt: the universal protein knowledgebase

(2016)   NUCLEIC ACIDS RESEARCH

HSP90 activity is required for MLKL oligomerisation and membrane translocation and the induction of necroptotic cell death

(2016) A V Jacobsen et al.   Cell Death & Disease

The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease

(2016) Daniela Lazzaretti et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure of protein O-mannose kinase reveals a unique active site architecture

(2016) Qinyu Zhu et al.   eLife

Relief of autoinhibition by conformational switch explains enzyme activation by a catalytically dead paralog

(2016) Oleg A Volkov et al.   eLife

The Tribbles 2 (TRB2) pseudokinase binds to ATP and autophosphorylates in a metal-independent manner

(2015) Fiona P. Bailey et al.   BIOCHEMICAL JOURNAL

Functional classification of CATH superfamilies: a domain-based approach for protein function annotation

(2015) Sayoni Das et al.   BIOINFORMATICS

A Single Kinase Generates the Majority of the Secreted Phosphoproteome

(2015) Vincent S. Tagliabracci et al.   CELL

Substrates Control Multimerization and Activation of the Multi-Domain ATPase Motor of Type VII Secretion

(2015) Oren S. Rosenberg et al.   CELL

Viral Pseudo-Enzymes Activate RIG-I via Deamidation to Evade Cytokine Production

(2015) Shanping He et al.   MOLECULAR CELL

Mitochondrial ADCK3 Employs an Atypical Protein Kinase-like Fold to Enable Coenzyme Q Biosynthesis

(2015) Jonathan A. Stefely et al.   MOLECULAR CELL

Structure and flexibility of the endosomal Vps34 complex reveals the basis of its function on membranes

(2015) K. Rostislavleva et al.   SCIENCE

Molecular Mechanism of CCAAT-Enhancer Binding Protein Recruitment by the TRIB1 Pseudokinase

(2015) James M. Murphy et al.   STRUCTURE

Structural and Thermodynamic Insights into Chitooligosaccharide Binding to Human Cartilage Chitinase 3-like Protein 2 (CHI3L2 or YKL-39)

(2014) Araya Ranok et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural and functional analysis of MiD51, a dynamin receptor required for mitochondrial fission

(2014) Viviane Richter et al.   JOURNAL OF CELL BIOLOGY

Crystal structure of the human COP9 signalosome

(2014) Gondichatnahalli M. Lingaraju et al.   NATURE

The structure of the Pan2–Pan3 core complex reveals cross-talk between deadenylase and pseudokinase

(2014) Ingmar B Schäfer et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure of the Rpn11–Rpn8 dimer reveals mechanisms of substrate deubiquitination during proteasomal degradation

(2014) Evan J Worden et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Crystal structure of the proteasomal deubiquitylation module Rpn8-Rpn11

(2014) G. R. Pathare et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of the pseudokinase-kinase domains from protein kinase TYK2 reveals a mechanism for Janus kinase (JAK) autoinhibition

(2014) P. J. Lupardus et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Xylose phosphorylation functions as a molecular switch to regulate proteoglycan biosynthesis

(2014) J. Wen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural analysis of the EGFR/HER3 heterodimer reveals the molecular basis for activating HER3 mutations

(2014) P. Littlefield et al.   Science Signaling

Day of the dead: pseudokinases and pseudophosphatases in physiology and disease

(2014) Veronika Reiterer et al.   TRENDS IN CELL BIOLOGY

Cell Fate Regulation Governed by a Repurposed Bacterial Histidine Kinase

(2014) W. Seth Childers et al.   PLOS BIOLOGY

The pseudo GTPase CENP-M drives human kinetochore assembly

(2014) Federica Basilico et al.   eLife

A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region

(2014) Courtney M Schroeder et al.   eLife

Insights into the evolution of divergent nucleotide-binding mechanisms among pseudokinases revealed by crystal structures of human and mouse MLKL

(2013) James M. Murphy et al.   BIOCHEMICAL JOURNAL

A robust methodology to subclassify pseudokinases based on their nucleotide-binding properties

(2013) James M. Murphy et al.   BIOCHEMICAL JOURNAL

Dawn of the dead: protein pseudokinases signal new adventures in cell biology

(2013) Patrick A. Eyers et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Mammalian iRhoms have distinct physiological functions including an essential role in TACE regulation

(2013) Yonka Christova et al.   EMBO REPORTS

Bradyzoite Pseudokinase 1 Is Crucial for Efficient Oral Infectivity of the Toxoplasma gondii Tissue Cyst

(2013) Kerry R. Buchholz et al.   EUKARYOTIC CELL

The CMT4B disease-causing phosphatases Mtmr2 and Mtmr13 localize to the Schwann cell cytoplasm and endomembrane compartments, where they depend upon each other to achieve wild-type levels of protein expression

(2013) Aubree A. Ng et al.   HUMAN MOLECULAR GENETICS

The Pseudokinase MLKL Mediates Necroptosis via a Molecular Switch Mechanism

(2013) James M. Murphy et al.   IMMUNITY

Trypanosoma brucei S-Adenosylmethionine Decarboxylase N Terminus Is Essential for Allosteric Activation by the Regulatory Subunit Prozyme

(2013) Nahir Velez et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Allosteric Activation of Trypanosomatid Deoxyhypusine Synthase by a Catalytically Dead Paralog

(2013) Suong Nguyen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Mechanism for KRIT1 Release of ICAP1-Mediated Suppression of Integrin Activation

(2013) Weizhi Liu et al.   MOLECULAR CELL

Pseudophosphatase STYX modulates cell-fate decisions and cell migration by spatiotemporal regulation of ERK1/2

(2013) V. Reiterer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of a bacterial putative acetyltransferase defines the fold of the human O-GlcNAcase C-terminal domain

(2013) F. V. Rao et al.   Open Biology

Human YKL-39 is a pseudo-chitinase with retained chitooligosaccharide-binding properties

(2012) Marianne Schimpl et al.   BIOCHEMICAL JOURNAL

The Vertebrate Mitotic Checkpoint Protein BUBR1 Is an Unusual Pseudokinase

(2012) Saskia J.E. Suijkerbuijk et al.   DEVELOPMENTAL CELL

Identification of Novel Host Cell Binding Partners of Oas1b, the Protein Conferring Resistance to Flavivirus-Induced Disease in Mice

(2012) S. C. Courtney et al.   JOURNAL OF VIROLOGY

Protein interaction data curation: the International Molecular Exchange (IMEx) consortium

(2012) Sandra Orchard et al.   NATURE METHODS

New lives for old: evolution of pseudoenzyme function illustrated by iRhoms

(2012) Colin Adrain et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Tumor Necrosis Factor Signaling Requires iRhom2 to Promote Trafficking and Activation of TACE

(2012) C. Adrain et al.   SCIENCE

Crystal Structure of the Human N-Myc Downstream-regulated Gene 2 Protein Provides Insight into Its Role as a Tumor Suppressor

(2011) Jungwon Hwang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The pseudokinase domain of JAK2 is a dual-specificity protein kinase that negatively regulates cytokine signaling

(2011) Daniela Ungureanu et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

ProKinO: An Ontology for Integrative Analysis of Protein Kinases in Cancer

(2011) Gurinder Gosal et al.   PLoS One

Mammalian Pragmin regulates Src family kinases via the Glu-Pro-Ile-Tyr-Ala (EPIYA) motif that is exploited by bacterial effectors

(2011) F. Safari et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Pseudokinases-remnants of evolution or key allosteric regulators?

(2010) Elton Zeqiraj et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Evolution of CASK into a Mg2+-Sensitive Kinase

(2010) K. Mukherjee et al.   Science Signaling

Stabilization of N-Myc Is a Critical Function of Aurora A in Human Neuroblastoma

(2009) Tobias Otto et al.   CANCER CELL

Caenorhabditis elegans caspase homolog CSP-2 inhibits CED-3 autoactivation and apoptosis in germ cells

(2009) X Geng et al.   CELL DEATH AND DIFFERENTIATION

PKC maturation is promoted by nucleotide pocket occupation independently of intrinsic kinase activity

(2009) Angus J M Cameron et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure of the LKB1-STRAD-MO25 Complex Reveals an Allosteric Mechanism of Kinase Activation

(2009) E. Zeqiraj et al.   SCIENCE

ATP and MO25α Regulate the Conformational State of the STRADα Pseudokinase and Activation of the LKB1 Tumour Suppressor

(2009) Elton Zeqiraj et al.   PLOS BIOLOGY

Inhibition of CED-3 zymogen activation and apoptosis in Caenorhabditis elegans by caspase homolog CSP-3

(2008) Xin Geng et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY