Synthetic Biology Goes Cell-Free

On-chip manufacturing of synthetic proteins for point-of-care therapeutics

(2019) Travis W. Murphy et al.   Microsystems & Nanoengineering

Artificial photosynthetic cell producing energy for protein synthesis

(2019) Samuel Berhanu et al.   Nature Communications

Hachimoji DNA and RNA: A genetic system with eight building blocks

(2019) Shuichi Hoshika et al.   SCIENCE

Expanding the palette of Streptomyces -based cell-free protein synthesis systems with enhanced yields

(2018) Jian Li et al.   BIOCHEMICAL ENGINEERING JOURNAL

Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production

(2018) Sheeja Jagadevan et al.   Biotechnology for Biofuels

Universal Chimeric Antigen Receptors for Multiplexed and Logical Control of T Cell Responses

(2018) Jang Hwan Cho et al.   CELL

Synthetic biology strategies for improving microbial synthesis of “green” biopolymers

(2018) Lisa A. Anderson et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Next-generation biocontainment systems for engineered organisms

(2018) Jeong Wook Lee et al.   Nature Chemical Biology

Generalized extracellular molecule sensor platform for programming cellular behavior

(2018) Leo Scheller et al.   Nature Chemical Biology

Rapid acquisition and model-based analysis of cell-free transcription–translation reactions from nonmodel bacteria

(2018) Simon J. Moore et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Programming gene and engineered-cell therapies with synthetic biology

(2018) Tasuku Kitada et al.   SCIENCE

Random heteropolymers preserve protein function in foreign environments

(2018) Brian Panganiban et al.   SCIENCE

Probiotic strains detect and suppress cholera in mice

(2018) Ning Mao et al.   Science Translational Medicine

Biosensing estrogenic endocrine disruptors in human blood and urine: A RAPID cell-free protein synthesis approach

(2018) Amin S.M. Salehi et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Engineering modular intracellular protein sensor-actuator devices

(2018) Velia Siciliano et al.   Nature Communications

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

(2018) Thapakorn Jaroentomeechai et al.   Nature Communications

Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids

(2018) Rey W. Martin et al.   Nature Communications

Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions

(2018) Ryota Sakamoto et al.   Scientific Reports

Rapid recombinant protein expression in cell-free extracts from human blood

(2018) David Burgenson et al.   Scientific Reports

Cell-Free Protein Synthesis From Fast-Growing Vibrio natriegens

(2018) Jurek Failmezger et al.   Frontiers in Microbiology

Industrial Applications of Enzymes: Recent Advances, Techniques, and Outlooks

(2018) Jordan Chapman et al.   Catalysts

Point-of-care production of therapeutic proteins of good-manufacturing-practice quality

(2018) Rajani Adiga et al.   Nature Biomedical Engineering

A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers

(2018) Melissa K. Takahashi et al.   Nature Communications

BioBits™ Explorer: A modular synthetic biology education kit

(2018) Ally Huang et al.   Science Advances

BioBits™ Bright: A fluorescent synthetic biology education kit

(2018) Jessica C. Stark et al.   Science Advances

Small-molecule-based regulation of RNA-delivered circuits in mammalian cells

(2018) Tyler E. Wagner et al.   Nature Chemical Biology

Paper-based colorimetric biosensor for antibiotics inhibiting bacterial protein synthesis

(2017) Tran Thi My Duyen et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Complex cellular logic computation using ribocomputing devices

(2017) Alexander A. Green et al.   NATURE

A semi-synthetic organism that stores and retrieves increased genetic information

(2017) Yorke Zhang et al.   NATURE

Trends in GPCR drug discovery: new agents, targets and indications

(2017) Alexander S. Hauser et al.   NATURE REVIEWS DRUG DISCOVERY

A synthetic biochemistry platform for cell free production of monoterpenes from glucose

(2017) Tyler P. Korman et al.   Nature Communications

Orthogonal Ribosome Biofirewall

(2017) Bin Jia et al.   ACS Synthetic Biology

Rapid and Scalable Preparation of Bacterial Lysates for Cell-Free Gene Expression

(2017) Andriy Didovyk et al.   ACS Synthetic Biology

Development of a CHO-Based Cell-Free Platform for Synthesis of Active Monoclonal Antibodies

(2017) Rey W. Martin et al.   ACS Synthetic Biology

A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples

(2017) Ke Yan Wen et al.   ACS Synthetic Biology

Nanomedicines: a theranostic approach for hepatocellular carcinoma

(2017) Afreen Usmani et al.   Artificial Cells Nanomedicine and Biotechnology

Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool

(2017) Simon J. Moore et al.   Biotechnology Journal

Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System

(2017) Mark Rustad et al.   Jove-Journal of Visualized Experiments

H2 -Fueled ATP Synthesis on an Electrode: Mimicking Cellular Respiration

(2016) Óscar Gutiérrez-Sanz et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Portable, On-Demand Biomolecular Manufacturing

(2016) Keith Pardee et al.   CELL

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

(2016) Keith Pardee et al.   CELL

Engineering Cellular Metabolism

(2016) Jens Nielsen et al.   CELL

Cell-Free Synthesis of a Functional Membrane Transporter into a Tethered Bilayer Lipid Membrane

(2016) Julius L. Zieleniecki et al.   LANGMUIR

Artificial cells: from basic science to applications

(2016) Can Xu et al.   Materials Today

A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery

(2016) Ashty S. Karim et al.   METABOLIC ENGINEERING

Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements

(2016) Richard Kelwick et al.   METABOLIC ENGINEERING

Synthetic biology to access and expand nature's chemical diversity

(2016) Michael J. Smanski et al.   NATURE REVIEWS MICROBIOLOGY

Cell-Free Synthetic Biology: Engineering Beyond the Cell

(2016) Jessica G. Perez et al.   Cold Spring Harbor Perspectives in Biology

Total biosynthesis of opiates by stepwise fermentation using engineered Escherichia coli

(2016) Akira Nakagawa et al.   Nature Communications

The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology

(2016) Jonathan Garamella et al.   ACS Synthetic Biology

LyophilizedEscherichia coli-based cell-free systems for robust, high-density, long-term storage

(2016) Mark Thomas Smith et al.   BIOTECHNIQUES

A cell-free system toward deciphering the post-translational modification barcodes of Oct4 in different cellular contexts

(2015) Songsong Dan et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

A versatile coupled cell-free transcription-translation system based on tobacco BY-2 cell lysates

(2015) Matthias Buntru et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

(2015) Amin S. M. Salehi et al.   Biotechnology Journal

A cell-free expression and purification process for rapid production of protein biologics

(2015) Challise J. Sullivan et al.   Biotechnology Journal

A simplified and robust protocol for immunoglobulin expression inEscherichia colicell-free protein synthesis systems

(2015) Qi Cai et al.   BIOTECHNOLOGY PROGRESS

Conceptual and methodological advances in cell-free directed evolution

(2015) Igor Dodevski et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Current strategies and findings in clinically relevant post-translational modification-specific proteomics

(2015) Oliver Pagel et al.   Expert Review of Proteomics

Antibody Conjugates with Unnatural Amino Acids

(2015) Trevor J. Hallam et al.   MOLECULAR PHARMACEUTICS

Protein synthesis in artificial cells: using compartmentalisation for spatial organisation in vesicle bioreactors

(2015) Yuval Elani et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

One-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil–water interfaces

(2015) Peter J. Yunker et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Assessing sequence plasticity of a virus-like nanoparticle by evolution toward a versatile scaffold for vaccines and drug delivery

(2015) Yuan Lu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Wheat germ cell-free expression: Two detergents with a low critical micelle concentration allow for production of soluble HCV membrane proteins

(2015) Marie-Laure Fogeron et al.   PROTEIN EXPRESSION AND PURIFICATION

Complete biosynthesis of opioids in yeast

(2015) S. Galanie et al.   SCIENCE

Robust production of recombinant phosphoproteins using cell-free protein synthesis

(2015) Javin P. Oza et al.   Nature Communications

High-throughput preparation methods of crude extract for robust cell-free protein synthesis

(2015) Yong-Chan Kwon et al.   Scientific Reports

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

(2015) Amin S. M. Salehi et al.   Biotechnology Journal

A cell-free expression and purification process for rapid production of protein biologics

(2015) Challise J. Sullivan et al.   Biotechnology Journal

Rapid cell-free forward engineering of novel genetic ring oscillators

(2015) Henrike Niederholtmeyer et al.   eLife

Reconstitution of the peptidoglycan cytoplasmic precursor biosynthetic pathway in cell-free system and rapid screening of antisense oligonucleotides for Mur enzymes

(2014) Jiayuan Sheng et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system

(2014) Filippo Caschera et al.   BIOCHIMIE

Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System

(2014) Erik S. Zimmerman et al.   BIOCONJUGATE CHEMISTRY

Cell-free metabolic engineering: Biomanufacturing beyond the cell

(2014) Quentin M. Dudley et al.   Biotechnology Journal

Toehold Switches: De-Novo-Designed Regulators of Gene Expression

(2014) Alexander A. Green et al.   CELL

Paper-Based Synthetic Gene Networks

(2014) Keith Pardee et al.   CELL

Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes

(2014) Rita Sachse et al.   FEBS LETTERS

Wheat germ systems for cell-free protein expression

(2014) Matthias Harbers   FEBS LETTERS

Engineering toward a bacterial “endoplasmic reticulum” for the rapid expression of immunoglobulin proteins

(2014) Dan Groff et al.   mAbs

Production of bispecific antibodies in “knobs-into-holes” using a cell-free expression system

(2014) Yiren Xu et al.   mAbs

A Continuous-Exchange Cell-Free Protein Synthesis System Based on Extracts from Cultured Insect Cells

(2014) Marlitt Stech et al.   PLoS One

Improved Cell-Free RNA and Protein Synthesis System

(2014) Jun Li et al.   PLoS One

Programmable bacteria detect and record an environmental signal in the mammalian gut

(2014) Jonathan W. Kotula et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vitro Fab display: a cell-free system for IgG discovery

(2014) R. L. Stafford et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A synthetic biochemistry system for thein vitroproduction of isoprene from glycolysis intermediates

(2014) Tyler P. Korman et al.   PROTEIN SCIENCE

Programmable on-chip DNA compartments as artificial cells

(2014) E. Karzbrun et al.   SCIENCE

The engineering of artificial cellular nanosystems using synthetic biology approaches

(2014) Fan Wu et al.   Wiley Interdisciplinary Reviews-Nanomedicine and Nanobiotechnology

A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

(2014) Zhiguang Zhu et al.   Nature Communications

Reconstitution of a 10-gene pathway for synthesis of the plant alkaloid dihydrosanguinarine in Saccharomyces cerevisiae

(2014) Elena Fossati et al.   Nature Communications

Rapidly Characterizing the Fast Dynamics of RNA Genetic Circuitry with Cell-Free Transcription–Translation (TX-TL) Systems

(2014) Melissa K. Takahashi et al.   ACS Synthetic Biology

Bacterial-based membrane protein production

(2013) Susan Schlegel et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Escherichia coli-based cell free production of flagellin and ordered flagellin display on virus-like particles

(2013) Yuan Lu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis

(2013) C. Eric Hodgman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Phage Renaissance: New Hope against Antibiotic Resistance

(2013) Carol Potera   ENVIRONMENTAL HEALTH PERSPECTIVES

Antibiotic resistance—the need for global solutions

(2013) Ramanan Laxminarayan et al.   LANCET INFECTIOUS DISEASES

Upgrading protein synthesis for synthetic biology

(2013) Patrick O'Donoghue et al.   Nature Chemical Biology

Statistical Experimental Design Guided Optimization of a One-Pot Biphasic Multienzyme Total Synthesis of Amorpha-4,11-diene

(2013) Xixian Chen et al.   PLoS One

In vitro evolution of  -hemolysin using a liposome display

(2013) S. Fujii et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production and stabilization of the trimeric influenza hemagglutinin stem domain for potentially broadly protective influenza vaccines

(2013) Y. Lu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecular chaperoning function of Ric-8 is to fold nascent heterotrimeric G protein   subunits

(2013) P. Chan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vitro affinity screening of protein and peptide binders by megavalent bead surface display

(2013) L. Diamante et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System

(2013) Zachary Z. Sun et al.   ACS Synthetic Biology

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

(2013) Zachary Z. Sun et al.   Jove-Journal of Visualized Experiments

Cell-free production of trimeric influenza hemagglutinin head domain proteins as vaccine antigens

(2012) John P. Welsh et al.   BIOTECHNOLOGY AND BIOENGINEERING

Expression without boundaries: Cell-free protein synthesis in pharmaceutical research

(2012) Marco G. Casteleijn et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system

(2012) Marlitt Stech et al.   JOURNAL OF BIOTECHNOLOGY

Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system

(2012) Gang Yin et al.   mAbs

Synthetic biology and the development of tools for metabolic engineering

(2012) Jay D. Keasling   METABOLIC ENGINEERING

Selection of a T7 promoter mutant with enhanced in vitro activity by a novel multi-copy bead display approach for in vitro evolution

(2012) Siddhartha Paul et al.   NUCLEIC ACIDS RESEARCH

Production of amorphadiene in yeast, and its conversion to dihydroartemisinic acid, precursor to the antimalarial agent artemisinin

(2012) P. J. Westfall et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Evolutionary selection of enzymatically synthesized semiconductors from biomimetic mineralization vesicles

(2012) L. A. Bawazer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A vaccine directed to B cells and produced by cell-free protein synthesis generates potent antilymphoma immunity

(2012) P. P. Ng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome Replication, Synthesis, and Assembly of the Bacteriophage T7 in a Single Cell-Free Reaction

(2012) Jonghyeon Shin et al.   ACS Synthetic Biology

Surface Functionalization of Virus-Like Particles by Direct Conjugation Using Azide−Alkyne Click Chemistry

(2011) Kedar G. Patel et al.   BIOCONJUGATE CHEMISTRY

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

Simplifying and streamlining Escherichia coli-based cell-free protein synthesis

(2011) William C. Yang et al.   BIOTECHNOLOGY PROGRESS

A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins

(2011) Cassandra Guarino et al.   GLYCOBIOLOGY

Cell-Free Expression and Assembly of ATP Synthase

(2011) Doreen Matthies et al.   JOURNAL OF MOLECULAR BIOLOGY

Traceless and Site-Specific Ubiquitination of Recombinant Proteins

(2011) Satpal Virdee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Peptide surfactants for cell-free production of functional G protein-coupled receptors

(2011) X. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells

(2011) Jonghyeon Shin et al.   ACS Synthetic Biology

Reconstruction of the UDP-N-acetylglucosamine biosynthetic pathway in cell-free system

(2010) Jiewen Zhou et al.   BIOTECHNOLOGY LETTERS

Efficacy of a Potential Trivalent Vaccine Based on Hc Fragments of Botulinum Toxins A, B, and E Produced in a Cell-Free Expression System

(2010) R. Zichel et al.   Clinical and Vaccine Immunology

Programming cells: towards an automated ‘Genetic Compiler’

(2010) Kevin Clancy et al.   CURRENT OPINION IN BIOTECHNOLOGY

Phage Therapy for Plant Disease Control

(2010) B. Balogh et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Enzymatic De Novo Pyrimidine Nucleotide Synthesis

(2010) Heather L. Schultheisz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Quantitative analysis and engineering of fatty acid biosynthesis in E. coli

(2010) Tiangang Liu et al.   METABOLIC ENGINEERING

Where microbiology meets microengineering: design and applications of reporter bacteria

(2010) Jan Roelof van der Meer et al.   NATURE REVIEWS MICROBIOLOGY

Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins

(2010) Friederike Junge et al.   New Biotechnology

In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme

(2010) Haruichi Asahara et al.   NUCLEIC ACIDS RESEARCH

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Site-specific incorporation of PEGylated amino acids into proteins using nonnatural amino acid mutagenesis

(2009) Naoki Shozen et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Pharmacological significance of glycosylation in therapeutic proteins

(2009) Huijuan Li et al.   CURRENT OPINION IN BIOTECHNOLOGY

Genetically EncodingNϵ-Methyl-l-lysine in Recombinant Histones

(2009) Duy P. Nguyen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Method for Genetically Installing Site-Specific Acetylation in Recombinant Histones Defines the Effects of H3 K56 Acetylation

(2009) Heinz Neumann et al.   MOLECULAR CELL

Spatially Resolved DNA Brushes on a Chip: Gene Activation by Enzymatic Cascade

(2009) Maya Bar et al.   NANO LETTERS

Synthetic Gene Networks That Count

(2009) A. E. Friedland et al.   SCIENCE

A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells

(2008) Yutetsu Kuruma et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

A highly efficient and economical cell-free protein synthesis system using the S12 extract of Escherichia coli

(2008) Tae-Wan Kim et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

An integrated cell-free metabolic platform for protein production and synthetic biology

(2008) Michael C Jewett et al.   Molecular Systems Biology

Efficient cell-free production of olfactory receptors: Detergent optimization, structure, and ligand binding analyses

(2008) L. Kaiser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Escherichia coli-based cell-free synthesis of virus-like particles

(2007) Bradley C. Bundy et al.   BIOTECHNOLOGY AND BIOENGINEERING

Production of yeast tRNA (m7G46) methyltransferase (Trm8–Trm82 complex) in a wheat germ cell-free translation system

(2007) Keisuke Matsumoto et al.   JOURNAL OF BIOTECHNOLOGY