Cell-free metabolic engineering: Biomanufacturing beyond the cell

Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system

(2014) Filippo Caschera et al.   BIOCHIMIE

Recent advances in engineering proteins for biocatalysis

(2014) Ye Li et al.   BIOTECHNOLOGY AND BIOENGINEERING

In vitro reconstitution of mevalonate pathway and targeted engineering of farnesene overproduction inEscherichia coli

(2014) Fayin Zhu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Toward cell-free biofuel production: Stable immobilization of oligomeric enzymes

(2014) J. Grimaldi et al.   BIOTECHNOLOGY PROGRESS

Cell-free synthesis of 1,3-propanediol from glycerol with a high yield

(2014) Fabian Rieckenberg et al.   ENGINEERING IN LIFE SCIENCES

Engineering synergy in biotechnology

(2014) Jens Nielsen et al.   Nature Chemical Biology

Yeast cell-free enzyme system for bio-ethanol production at elevated temperatures

(2014) Waleed Ahmad Khattak et al.   PROCESS BIOCHEMISTRY

A synthetic biochemistry system for thein vitroproduction of isoprene from glycolysis intermediates

(2014) Tyler P. Korman et al.   PROTEIN SCIENCE

A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

(2014) Zhiguang Zhu et al.   Nature Communications

A synthetic biochemistry molecular purge valve module that maintains redox balance

(2014) Paul H. Opgenorth et al.   Nature Communications

Gene Circuit Performance Characterization and Resource Usage in a Cell-Free “Breadboard”

(2014) Dan Siegal-Gaskins et al.   ACS Synthetic Biology

High-Yield Production of Dihydrogen from Xylose by Using a Synthetic Enzyme Cascade in a Cell-Free System

(2013) Julia S. Martín del Campo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Development of a Continuous Bioconversion System Using a Thermophilic Whole-Cell Biocatalyst

(2013) Pham Huynh Ninh et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Flux analysis and metabolomics for systematic metabolic engineering of microorganisms

(2013) Yoshihiro Toya et al.   BIOTECHNOLOGY ADVANCES

The good of two worlds: increasing complexity in cell-free systems

(2013) Sonja Billerbeck et al.   CURRENT OPINION IN BIOTECHNOLOGY

Molecular tools for chemical biotechnology

(2013) Stephanie Galanie et al.   CURRENT OPINION IN BIOTECHNOLOGY

Metabolic engineering of yeast for production of fuels and chemicals

(2013) Jens Nielsen et al.   CURRENT OPINION IN BIOTECHNOLOGY

Cell-free translation of peptides and proteins:from high throughput screening to clinical production

(2013) Christopher J Murray et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Improvement of thermostable aldehyde dehydrogenase by directed evolution for application in Synthetic Cascade Biomanufacturing

(2013) Fabian Steffler et al.   ENZYME AND MICROBIAL TECHNOLOGY

New biotechnology paradigm: cell-free biosystems for biomanufacturing

(2013) Joseph A. Rollin et al.   GREEN CHEMISTRY

In vitro production of n-butanol from glucose

(2013) Borimas Krutsakorn et al.   METABOLIC ENGINEERING

Construction of an in vitro bypassed pyruvate decarboxylation pathway using thermostable enzyme modules and its application to N-acetylglutamate production

(2013) Borimas Krutsakorn et al.   Microbial Cell Factories

High-level semi-synthetic production of the potent antimalarial artemisinin

(2013) C. J. Paddon et al.   NATURE

Synthetic non-oxidative glycolysis enables complete carbon conservation

(2013) Igor W. Bogorad et al.   NATURE

Metabolic tinker: an online tool for guiding the design of synthetic metabolic pathways

(2013) Kent McClymont et al.   NUCLEIC ACIDS RESEARCH

Non-Complexed Four Cascade Enzyme Mixture: Simple Purification and Synergetic Co-stabilization

(2013) Suwan Myung et al.   PLoS One

Refolding of a Thermostable Glyceraldehyde Dehydrogenase for Application in Synthetic Cascade Biomanufacturing

(2013) Fabian Steffler et al.   PLoS One

Statistical Experimental Design Guided Optimization of a One-Pot Biphasic Multienzyme Total Synthesis of Amorpha-4,11-diene

(2013) Xixian Chen et al.   PLoS One

Enzymatic transformation of nonfood biomass to starch

(2013) C. You et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide

(2013) M. W. Keller et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Retropath: Automated Pipeline for Embedded Metabolic Circuits

(2013) Pablo Carbonell et al.   ACS Synthetic Biology

Recyclable cellulose-containing magnetic nanoparticles: immobilization of cellulose-binding module-tagged proteins and a synthetic metabolon featuring substrate channeling

(2013) Suwan Myung et al.   Journal of Materials Chemistry B

In silico assessment of cell-free systems

(2012) Matthias Bujara et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cell-free protein synthesis: the state of the art

(2012) James W. Whittaker   BIOTECHNOLOGY LETTERS

Preparative, in Vitro Biocatalysis of Triketide Lactone Chiral Building Blocks

(2012) Andrew D. Harper et al.   CHEMBIOCHEM

Multienzyme Whole-Cell In Situ Biocatalysis for the Production of Flaviolin in Permeabilized Cells of Escherichia coli

(2012) Steffen Krauser et al.   ChemCatChem

In silico evaluation of a complex multi-enzymatic system using one-pot and modular approaches: Application to the high-yield production of hydrogen from a synthetic metabolic pathway

(2012) Inés Ardao et al.   CHEMICAL ENGINEERING SCIENCE

Cell-Free Metabolic Engineering: Production of Chemicals by Minimized Reaction Cascades

(2012) Jan-Karl Guterl et al.   ChemSusChem

Depletion of fossil fuels and anthropogenic climate change—A review

(2012) Mikael Höök et al.   ENERGY POLICY

Biosynthesis “debugged”: Novel bioproduction strategies

(2012) Jan-Karl Guterl et al.   ENGINEERING IN LIFE SCIENCES

Direct conversion of glucose to malate by synthetic metabolic engineering

(2012) Xiaoting Ye et al.   JOURNAL OF BIOTECHNOLOGY

Computational tools for metabolic engineering

(2012) Wilbert B. Copeland et al.   METABOLIC ENGINEERING

Expanding the chemical palate of cells by combining systems biology and metabolic engineering

(2012) Kathleen A. Curran et al.   METABOLIC ENGINEERING

The future of metabolic engineering and synthetic biology: Towards a systematic practice

(2012) Vikramaditya G. Yadav et al.   METABOLIC ENGINEERING

Synthetic biology and the development of tools for metabolic engineering

(2012) Jay D. Keasling   METABOLIC ENGINEERING

Synthetic metabolic engineering-a novel, simple technology for designing a chimeric metabolic pathway

(2012) Xiaoting Ye et al.   Microbial Cell Factories

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Natural strategies for the spatial optimization of metabolism in synthetic biology

(2012) Christina M Agapakis et al.   Nature Chemical Biology

Systems metabolic engineering of microorganisms for natural and non-natural chemicals

(2012) Jeong Wook Lee et al.   Nature Chemical Biology

Customized optimization of metabolic pathways by combinatorial transcriptional engineering

(2012) Jing Du et al.   NUCLEIC ACIDS RESEARCH

Multiplexed in Vivo His-Tagging of Enzyme Pathways for in Vitro Single-Pot Multienzyme Catalysis

(2012) Harris H. Wang et al.   ACS Synthetic Biology

Self-Assembly of Synthetic Metabolons through Synthetic Protein Scaffolds: One-Step Purification, Co-immobilization, and Substrate Channeling

(2012) Chun You et al.   ACS Synthetic Biology

Transforming biochemical engineering with cell-free biology

(2011) James R. Swartz   AICHE JOURNAL

Tuning Gene Expression in Yarrowia lipolytica by a Hybrid Promoter Approach

(2011) John Blazeck et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Substrate channeling and enzyme complexes for biotechnological applications

(2011) Y.-H. Percival Zhang   BIOTECHNOLOGY ADVANCES

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

Biohydrogenation from Biomass Sugar Mediated by In Vitro Synthetic Enzymatic Pathways

(2011) Yiran Wang et al.   CHEMISTRY & BIOLOGY

Fermentative production of butanol—the industrial perspective

(2011) Edward M Green   CURRENT OPINION IN BIOTECHNOLOGY

Synthesis of non-natural carbohydrates from glycerol and aldehydes in a one-pot four-enzyme cascade reaction

(2011) Lara Babich et al.   GREEN CHEMISTRY

Scale-up of microbial processes: Impacts, tools and open questions

(2011) R. Takors   JOURNAL OF BIOTECHNOLOGY

Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli

(2011) Sabine Bastian et al.   METABOLIC ENGINEERING

Cell-free synthetic biology: Thinking outside the cell

(2011) C. Eric Hodgman et al.   METABOLIC ENGINEERING

Optimization of a blueprint for in vitro glycolysis by metabolic real-time analysis

(2011) Matthias Bujara et al.   Nature Chemical Biology

The University of Minnesota Pathway Prediction System: multi-level prediction and visualization

(2011) J. Gao et al.   NUCLEIC ACIDS RESEARCH

DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency

(2011) Robert J. Conrado et al.   NUCLEIC ACIDS RESEARCH

Biotechnological Development of a Practical Synthesis of Ethyl (S)-2-Ethoxy-3-(p-methoxyphenyl)propanoate (EEHP): Over 100-Fold Productivity Increase from Yeast Whole Cells to Recombinant Isolated Enzymes

(2011) Matthias Bechtold et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

In vitro reconstitution and steady-state analysis of the fatty acid synthase from Escherichia coli

(2011) X. Yu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Prolonged production of proteins in a cell-free protein synthesis system using polymeric carbohydrates as an energy source

(2011) Ho-Cheol Kim et al.   PROCESS BIOCHEMISTRY

What is vital (and not vital) to advance economically-competitive biofuels production

(2011) Y.-H. Percival Zhang   PROCESS BIOCHEMISTRY

From polymeric nanoreactors to artificial organelles

(2011) Ruud J. R. W. Peters et al.   Chemical Science

Engineering of Biocatalysts: from Evolution to Creation

(2011) Maureen B. Quin et al.   ACS Catalysis

An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells

(2011) Jonghyeon Shin et al.   ACS Synthetic Biology

Broadening the cofactor specificity of a thermostable alcohol dehydrogenase using rational protein design introduces novel kinetic transient behavior

(2010) Elliot Campbell et al.   BIOTECHNOLOGY AND BIOENGINEERING

Engineering in complex systems

(2010) Matthias Bujara et al.   CURRENT OPINION IN BIOTECHNOLOGY

The biorefinery concept: Using biomass instead of oil for producing energy and chemicals

(2010) Francesco Cherubini   ENERGY CONVERSION AND MANAGEMENT

Technology development for the production of biobased products from biorefinery carbohydrates—the US Department of Energy’s “Top 10” revisited

(2010) Joseph J. Bozell et al.   GREEN CHEMISTRY

Engineering Cofactor Preference of Ketone Reducing Biocatalysts: A Mutagenesis Study on a γ-Diketone Reductase from the Yeast Saccharomyces cerevisiae Serving as an Example

(2010) Michael Katzberg et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential of artificial bio-synthetic pathway using thermophilic enzymes

(2010) Kohsuke Honda et al.   JOURNAL OF BIOTECHNOLOGY

Enzymatic De Novo Pyrimidine Nucleotide Synthesis

(2010) Heather L. Schultheisz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Evolution, genomic analysis, and reconstruction of isobutanol tolerance in Escherichia coli

(2010) Shota Atsumi et al.   Molecular Systems Biology

Five hard truths for synthetic biology

(2010) Roberta Kwok   NATURE

Microbial electrosynthesis — revisiting the electrical route for microbial production

(2010) Korneel Rabaey et al.   NATURE REVIEWS MICROBIOLOGY

PathPred: an enzyme-catalyzed metabolic pathway prediction server

(2010) Y. Moriya et al.   NUCLEIC ACIDS RESEARCH

Enzymes and Bioconversions of Industrial, Pharmaceutical, and Biotechnological Significance

(2010) Sergio Sanchez et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Manufacturing Molecules Through Metabolic Engineering

(2010) J. D. Keasling   SCIENCE

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Cell-free protein synthesis energized by slowly-metabolized maltodextrin

(2009) Yiran Wang et al.   BMC BIOTECHNOLOGY

Spontaneous High-Yield Production of Hydrogen from Cellulosic Materials and Water Catalyzed by Enzyme Cocktails

(2009) Xinhao Ye et al.   ChemSusChem

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Synthetic protein scaffolds provide modular control over metabolic flux

(2009) John E Dueber et al.   NATURE BIOTECHNOLOGY

Pathway Engineered Enzymaticde NovoPurine Nucleotide Synthesis

(2008) Heather L. Schultheisz et al.   ACS Chemical Biology

An integrated cell-free metabolic platform for protein production and synthetic biology

(2008) Michael C Jewett et al.   Molecular Systems Biology