Wheat germ systems for cell-free protein expression

Synthesis and site-directed fluorescence labeling of azido proteins using eukaryotic cell-free orthogonal translation systems

(2014) Robert B. Quast et al.   ANALYTICAL BIOCHEMISTRY

Profiling of Autoantibodies in Sera of Pancreatic Cancer Patients

(2014) Yosuke Nagayoshi et al.   ANNALS OF SURGICAL ONCOLOGY

Combinatory use of cell-free protein expression, limited proteolysis and mass spectrometry for the high-throughput protein domain identification

(2014) Xinchun Shen et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System

(2014) Erik S. Zimmerman et al.   BIOCONJUGATE CHEMISTRY

Detection of tumor-associated antigens in culture supernatants using autoantibodiesin sera from patients with bladder cancer

(2014) Sho MINAMI et al.   BIOMEDICAL RESEARCH-TOKYO

Co-regulation proteomics reveals substrates and mechanisms of APC/C-dependent degradation

(2014) S. A. Singh et al.   EMBO JOURNAL

Incorporation of adenine nucleotide transporter, Ant1p, into proteoliposomes facilitates ATP translocation and activation of encapsulated luciferase

(2014) Akira Nozawa et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Virus-like particles: the future of microbial factories and cell-free systems as platforms for vaccine development

(2013) William A Rodríguez-Limas et al.   CURRENT OPINION IN BIOTECHNOLOGY

Cell-free translation of peptides and proteins:from high throughput screening to clinical production

(2013) Christopher J Murray et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Cell-free expression—making a mark

(2013) Frank Bernhard et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Application of wheat germ cell-free protein expression system for novel malaria vaccine candidate discovery

(2013) Thangavelu U Arumugam et al.   Expert Review of Vaccines

Engine out of the chassis: Cell-free protein synthesis and its uses

(2013) Gabriel Rosenblum et al.   FEBS LETTERS

Functional Comparison of Plasmodium falciparum Transmission-Blocking Vaccine Candidates by the Standard Membrane-Feeding Assay

(2013) Kazutoyo Miura et al.   INFECTION AND IMMUNITY

Close allies in membrane protein research: Cell-free synthesis and nanotechnology

(2013) Nadim Shadiac et al.   MOLECULAR MEMBRANE BIOLOGY

Applications of Azide-Based Bioorthogonal Click Chemistry in Glycobiology

(2013) Xiu Zhang et al.   MOLECULES

DNASU plasmid and PSI:Biology-Materials repositories: resources to accelerate biological research

(2013) Catherine Y. Seiler et al.   NUCLEIC ACIDS RESEARCH

The myristoylated amino-terminus of an Arabidopsis calcium-dependent protein kinase mediates plasma membrane localization

(2013) Sheen X. Lu et al.   PLANT MOLECULAR BIOLOGY

Combining Functional and Structural Genomics to Sample the Essential Burkholderia Structome

(2013) Loren Baugh et al.   PLoS One

Molecular chaperoning function of Ric-8 is to fold nascent heterotrimeric G protein   subunits

(2013) P. Chan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Characterization of the human ESC transcriptome by hybrid sequencing

(2013) K. F. Au et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Recombinant expression, detergent solubilisation and purification of insect odorant receptor subunits

(2013) Colm Carraher et al.   PROTEIN EXPRESSION AND PURIFICATION

To fuse or not to fuse: What is your purpose?

(2013) Mark R. Bell et al.   PROTEIN SCIENCE

ESPRESSO: A system for estimating protein expression and solubility in protein expression systems

(2013) Shuichi Hirose et al.   PROTEOMICS

High-throughput cloning and expression library creation for functional proteomics

(2013) Fernanda Festa et al.   PROTEOMICS

A cell-free approach to accelerate the study of protein-protein interactions in vitro

(2013) E. Sierecki et al.   Interface Focus

Function of Shaker potassium channels produced by cell-free translation upon injection into Xenopus oocytes

(2013) Brian W. Jarecki et al.   Scientific Reports

Several Affinity Tags Commonly Used in Chromatographic Purification

(2013) Xinyu Zhao et al.   Journal of Analytical Methods in Chemistry

Development of oligomannose-coated liposome-based nasal vaccine against human parainfluenza virus type 3

(2013) Kyosuke Senchi et al.   Frontiers in Microbiology

Establishment of a robust dengue virus NS3–NS5 binding assay for identification of protein–protein interaction inhibitors

(2012) Hirotaka Takahashi et al.   ANTIVIRAL RESEARCH

Cell-free protein synthesis of membrane (1,3)-β-d-glucan (curdlan) synthase: Co-translational insertion in liposomes and reconstitution in nanodiscs

(2012) Agalya Periasamy et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Population transcriptomics with single-cell resolution: A new field made possible by microfluidics

(2012) Charles Plessy et al.   BIOESSAYS

Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications

(2012) Carissa L. Young et al.   Biotechnology Journal

Efficient Decoding of the UAG Triplet as a Full-Fledged Sense Codon Enhances the Growth of a prfA-Deficient Strain of Escherichia coli

(2012) K. Ohtake et al.   JOURNAL OF BACTERIOLOGY

Structural Basis for Specific Recognition of Rpt1p, an ATPase Subunit of 26 S Proteasome, by Proteasome-dedicated Chaperone Hsm3p

(2012) Kenji Takagi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

On-Chip Synthesis of Protein Microarrays from DNA Microarrays via Coupled In Vitro Transcription and Translation for Surface Plasmon Resonance Imaging Biosensor Applications

(2012) Ting H. Seefeld et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Hybrid error correction and de novo assembly of single-molecule sequencing reads

(2012) Sergey Koren et al.   NATURE BIOTECHNOLOGY

FLEXIQinase, a mass spectrometry–based assay, to unveil multikinase mechanisms

(2012) Sasha A Singh et al.   NATURE METHODS

Selected reaction monitoring–based proteomics: workflows, potential, pitfalls and future directions

(2012) Paola Picotti et al.   NATURE METHODS

Flexible Programming of Cell-Free Protein Synthesis Using Magnetic Bead-Immobilized Plasmids

(2012) Ka-Young Lee et al.   PLoS One

Advancing the sensitivity of selected reaction monitoring-based targeted quantitative proteomics

(2012) Tujin Shi et al.   PROTEOMICS

Cell-free production of integral membrane aspartic acid proteases reveals zinc-dependent methyltransferase activity of thePseudomonas aeruginosaprepilin peptidase PilD

(2012) Khaled A. Aly et al.   MicrobiologyOpen

Wheat germ cell-free expression system as a pathway to improve protein yield and solubility for the SSGCID pipeline

(2011) Katherine Guild et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

Surface Functionalization of Virus-Like Particles by Direct Conjugation Using Azide−Alkyne Click Chemistry

(2011) Kedar G. Patel et al.   BIOCONJUGATE CHEMISTRY

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system

(2011) Akira Nozawa et al.   BMC BIOTECHNOLOGY

Fabrication optimization of a miniaturized array device for cell-free protein synthesis

(2011) Ruba Khnouf et al.   ELECTROPHORESIS

Statistical analysis of features associated with protein expression/solubility in an in vivo Escherichia coli expression system and a wheat germ cell-free expression system

(2011) Shuichi Hirose et al.   JOURNAL OF BIOCHEMISTRY

Hydrogen exchange during cell-free incorporation of deuterated amino acids and an approach to its inhibition

(2011) Marco Tonelli et al.   JOURNAL OF BIOMOLECULAR NMR

Firm wheat-germ cell-free system with extended vector usage for high-throughput protein screening

(2011) SungGa Lee et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Discovery of Improved EGF Agonists Using a Novel In Vitro Screening Platform

(2011) Bertrand H. Lui et al.   JOURNAL OF MOLECULAR BIOLOGY

Leishmania cell-free protein expression system

(2011) Oleksiy Kovtun et al.   METHODS

Autophosphorylation profiling of Arabidopsis protein kinases using the cell-free system

(2011) Keiichirou Nemoto et al.   PHYTOCHEMISTRY

Cell-Free Protein Expression under Macromolecular Crowding Conditions

(2011) Xumeng Ge et al.   PLoS One

Optimized clinical performance of growth hormone with an expanded genetic code

(2011) H. Cho et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient production and purification of functional bacteriorhodopsin with a wheat-germ cell-free system and a combination of Fos-choline and CHAPS detergents

(2010) Takahisa Genji et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins

(2010) Satoko Matsunaga et al.   BMC BIOTECHNOLOGY

The consensus motif for N-myristoylation of plant proteins in a wheat germ cell-free translation system

(2010) Seiji Yamauchi et al.   FEBS Journal

Cell-free Synthesis and Functional Characterization of Sphingolipid Synthases from Parasitic Trypanosomatid Protozoa

(2010) Elitza S. Sevova et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Beyond the Canonical 20 Amino Acids: Expanding the Genetic Lexicon

(2010) Travis S. Young et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Comparative Analysis of Human Src-Family Kinase Substrate Specificity in Vitro

(2010) Hiroyuki Takeda et al.   JOURNAL OF PROTEOME RESEARCH

Simple Screening Method for Autoantigen Proteins Using the N-Terminal Biotinylated Protein Library Produced by Wheat Cell-Free Synthesis

(2010) Kazuhiro Matsuoka et al.   JOURNAL OF PROTEOME RESEARCH

Wheat-germ cell-free production of prion proteins for solid-state NMR structural studies

(2010) Claire Noirot et al.   New Biotechnology

Robotic large-scale application of wheat cell-free translation to structural studies including membrane proteins

(2010) Emily T. Beebe et al.   New Biotechnology

Development and evaluation of data-driven designed tags (DDTs) for controlling protein solubility

(2010) Shuichi Hirose et al.   New Biotechnology

Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System

(2010) Oleksiy Kovtun et al.   PLoS One

Control of translational initiation in the wheat-embryo cell-free protein expression system for producing homogenous products

(2010) Takashi Ohta et al.   PROTEIN EXPRESSION AND PURIFICATION

Construction of a Protein Library of Arabidopsis Transcription Factors Using a Wheat Cell-Free Protein Production System and Its Application for DNA Binding Analysis

(2009) Akira NOZAWA et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis

(2009) Hirotaka Takahashi et al.   BMC PLANT BIOLOGY

Computational analysis of membrane proteins: the largest class of drug targets

(2009) Yalini Arinaminpathy et al.   DRUG DISCOVERY TODAY

The completion of the Mammalian Gene Collection (MGC)

(2009) et al.   GENOME RESEARCH

FLEXIQuant: A Novel Tool for the Absolute Quantification of Proteins, and the Simultaneous Identification and Quantification of Potentially Modified Peptides

(2009) Sasha Singh et al.   JOURNAL OF PROTEOME RESEARCH

Membrane protein expression: no cells required

(2009) Federico Katzen et al.   TRENDS IN BIOTECHNOLOGY

A set of ligation-independent in vitro translation vectors for eukaryotic protein production

(2008) Viola Bardóczy et al.   BMC BIOTECHNOLOGY

DNA-binding profiling of human hormone nuclear receptors via fluorescence correlation spectroscopy in a cell-free system

(2008) Tamiyo Kobayashi et al.   FEBS LETTERS

The current status of cDNA cloning

(2008) Matthias Harbers   GENOMICS

Wheat Germ Cell-Free System-Based Production of Malaria Proteins for Discovery of Novel Vaccine Candidates

(2008) T. Tsuboi et al.   INFECTION AND IMMUNITY

Human protein factory for converting the transcriptome into an in vitro–expressed proteome

(2008) Naoki Goshima et al.   NATURE METHODS

Human Gene and Protein Database (HGPD): a novel database presenting a large quantity of experiment-based results in human proteomics

(2008) Y. Maruyama et al.   NUCLEIC ACIDS RESEARCH

Efficient cell-free production of olfactory receptors: Detergent optimization, structure, and ligand binding analyses

(2008) L. Kaiser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex

(2008) Michael A. Goren et al.   PROTEIN EXPRESSION AND PURIFICATION