Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes

Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system

(2014) Srujan Kumar Dondapati et al.   BIOSENSORS & BIOELECTRONICS

Giant vesicles functionally expressing membrane receptors for an insect pheromone

(2014) Satoshi Hamada et al.   CHEMICAL COMMUNICATIONS

Integral Membrane Protein Fragment Recombination after Transfer from Nanolipoprotein Particles to Bicelles

(2013) Ginny Lai et al.   BIOCHEMISTRY

Cell-free synthesis of membrane proteins: Tailored cell models out of microsomes

(2013) Susanne F. Fenz et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Cell-free protein expression based on extracts from CHO cells

(2013) Andreas K. Brödel et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cell-Free Preparation of Functional and Triggerable Giant Proteoliposomes

(2013) Yan-Jun Liu et al.   CHEMBIOCHEM

Enhanced functional expression of aquaporin Z via fusion of in situ cleavable leader peptides in Escherichia coli cell-free system

(2013) Xu Zhang et al.   ENZYME AND MICROBIAL TECHNOLOGY

Colocalized Delivery of Adjuvant and Antigen Using Nanolipoprotein Particles Enhances the Immune Response to Recombinant Antigens

(2013) Nicholas O. Fischer et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Detergent-free mass spectrometry of membrane protein complexes

(2013) Jonathan T S Hopper et al.   NATURE METHODS

Optimization of the design and preparation of nanoscale phospholipid bilayers for its application to solution NMR

(2013) Robbins Puthenveetil et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Cell-free synthesis of functional thermostable direct hemolysins of Vibrio parahaemolyticus

(2013) Silke Bechlars et al.   TOXICON

Function of Shaker potassium channels produced by cell-free translation upon injection into Xenopus oocytes

(2013) Brian W. Jarecki et al.   Scientific Reports

Nonionic Homopolymeric Amphipols: Application to Membrane Protein Folding, Cell-Free Synthesis, and Solution Nuclear Magnetic Resonance

(2012) Paola Bazzacco et al.   BIOCHEMISTRY

Cell-free protein synthesis of membrane (1,3)-β-d-glucan (curdlan) synthase: Co-translational insertion in liposomes and reconstitution in nanodiscs

(2012) Agalya Periasamy et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

The Magic of Bicelles Lights Up Membrane Protein Structure

(2012) Ulrich H. N. Dürr et al.   CHEMICAL REVIEWS

Synthesis of membrane proteins in eukaryotic cell-free systems

(2012) Rita Sachse et al.   ENGINEERING IN LIFE SCIENCES

The Cell-Free Integration of a Polytopic Mitochondrial Membrane Protein into Liposomes Occurs Cotranslationally and in a Lipid-Dependent Manner

(2012) Ashley R. Long et al.   PLoS One

Distinct roles of metabotropic glutamate receptor dimerization in agonist activation and G-protein coupling

(2012) D. El Moustaine et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production of UCP1 a membrane protein from the inner mitochondrial membrane using the cell free expression system in the presence of a fluorinated surfactant

(2011) Iulia Blesneac et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Lipid–protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: Comparison with detergent micelles, bicelles and liposomes

(2011) E.N. Lyukmanova et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system

(2011) Akira Nozawa et al.   BMC BIOTECHNOLOGY

Bilayer Hydrophobic Thickness and Integral Membrane Protein Function

(2011) Larisa E. Cybulski et al.   CURRENT PROTEIN & PEPTIDE SCIENCE

A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins

(2011) Cassandra Guarino et al.   GLYCOBIOLOGY

Preparative Scale Cell-free Production and Quality Optimization of MraY Homologues in Different Expression Modes

(2011) Yi Ma et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cell-Free Expression and Assembly of ATP Synthase

(2011) Doreen Matthies et al.   JOURNAL OF MOLECULAR BIOLOGY

Green Proteorhodopsin Reconstituted into Nanoscale Phospholipid Bilayers (Nanodiscs) as Photoactive Monomers

(2011) Matthew J. Ranaghan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems

(2011) Karolina Corin et al.   PLoS One

Peptide surfactants for cell-free production of functional G protein-coupled receptors

(2011) X. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell-free synthesis of membrane subunits of ATP synthase in phospholipid bicelles: NMR shows subunitafold similar to the protein in the cell membrane

(2011) Eva-Maria E. Uhlemann et al.   PROTEIN SCIENCE

Coupled cell-free synthesis and lipid vesicle insertion of a functional oligomeric channel MscL

(2010) Catherine Berrier et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Conjugation to Nickel-Chelating Nanolipoprotein Particles Increases the Potency and Efficacy of Subunit Vaccines to Prevent West Nile Encephalitis

(2010) Nicholas O. Fischer et al.   BIOCONJUGATE CHEMISTRY

Update on designing and building minimal cells

(2010) Michael C Jewett et al.   CURRENT OPINION IN BIOTECHNOLOGY

Direct integration of cell-free-synthesized connexin-43 into liposomes and hemichannel formation

(2010) Yuki Moritani et al.   FEBS Journal

In vitro synthesis and oligomerization of the mechanosensitive channel of large conductance, MscL, into a functional ion channel

(2010) Claire E. Price et al.   FEBS LETTERS

Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: A case study of the human endothelin A receptor

(2010) Friederike Junge et al.   JOURNAL OF STRUCTURAL BIOLOGY

Photoelectrochemical complexes for solar energy conversion that chemically and autonomously regenerate

(2010) Moon-Ho Ham et al.   Nature Chemistry

Teaching old receptors new tricks: biasing seven-transmembrane receptors

(2010) Sudarshan Rajagopal et al.   NATURE REVIEWS DRUG DISCOVERY

In the cauldron of cell-free synthesis of membrane proteins: playing with new surfactants

(2010) Kyu-Ho Park et al.   New Biotechnology

Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins

(2010) Friederike Junge et al.   New Biotechnology

Cell-free synthesis and characterization of a novel cytotoxic pierisin-like protein from the cabbage butterfly Pieris rapae

(2010) Joachim H.C. Orth et al.   TOXICON

The nanodisc: a novel tool for membrane protein studies

(2009) Jonas Borch et al.   BIOLOGICAL CHEMISTRY

Direct formation of proteo-liposomes by in vitro synthesis and cellular cytosolic delivery with connexin-expressing liposomes

(2009) Makoto Kaneda et al.   BIOMATERIALS

Cell-free synthesis of functional aquaporin Z in synthetic liposomes

(2009) Norman T. Hovijitra et al.   BIOTECHNOLOGY AND BIOENGINEERING

Protein Incorporation in Giant Lipid Vesicles under Physiological Conditions

(2009) Paige M. Shaklee et al.   CHEMBIOCHEM

Membrane protein assembly into Nanodiscs

(2009) Timothy H. Bayburt et al.   FEBS LETTERS

G-protein-coupled receptors, cholesterol and palmitoylation: facts about fats

(2009) Bice Chini et al.   JOURNAL OF MOLECULAR ENDOCRINOLOGY

Emerging roles for lipids in shaping membrane-protein function

(2009) Rob Phillips et al.   NATURE

Bimodal protein solubility distribution revealed by an aggregation analysis of the entire ensemble of Escherichia coli proteins

(2009) T. Niwa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production of functional bacteriorhodopsin by anEscherichia colicell-free protein synthesis system supplemented with steroid detergent and lipid

(2009) Kazumi Shimono et al.   PROTEIN SCIENCE

Analysis of the membrane proteome of canine pancreatic rough microsomes identifies a novel Hsp40, termed ERj7

(2009) René P. Zahedi et al.   PROTEOMICS

Membrane protein expression: no cells required

(2009) Federico Katzen et al.   TRENDS IN BIOTECHNOLOGY

A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells

(2008) Yutetsu Kuruma et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Liposomes-mediated delivery of pro-apoptotic therapeutic membrane proteins

(2008) Lavinia Liguori et al.   JOURNAL OF CONTROLLED RELEASE

In Vitro Multimerization and Membrane Insertion of Bacterial Outer Membrane Secretin PulD

(2008) Ingrid Guilvout et al.   JOURNAL OF MOLECULAR BIOLOGY

Droplet interface bilayers

(2008) Hagan Bayley et al.   Molecular BioSystems

An integrated cell-free metabolic platform for protein production and synthetic biology

(2008) Michael C Jewett et al.   Molecular Systems Biology

Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex

(2008) Michael A. Goren et al.   PROTEIN EXPRESSION AND PURIFICATION

An efficient in vitro translation system from mammalian cells lacking the translational inhibition caused by eIF2 phosphorylation

(2008) V. V. Zeenko et al.   RNA

Direct preparation of giant proteo-liposomes by in vitro membrane protein synthesis

(2007) Shin-ichiro M. Nomura et al.   JOURNAL OF BIOTECHNOLOGY

Development of a Minimal Cell-Free Translation System for the Synthesis of Presecretory and Integral Membrane Proteins

(2005) Yutetsu Kuruma et al.   BIOTECHNOLOGY PROGRESS