Optical clearing methods: An overview of the techniques used for the imaging of 3D spheroids

FDISCO: Advanced solvent-based clearing method for imaging whole organs

(2019) Yisong Qi et al.   Science Advances

A three-dimensional neural spheroid model for capillary-like network formation

(2018) Molly E. Boutin et al.   JOURNAL OF NEUROSCIENCE METHODS

Rapid spheroid clearing on a microfluidic chip

(2018) Tomas Silva Santisteban et al.   LAB ON A CHIP

Clear T immersion optical clearing method for intact 3D spheroids imaging through confocal laser scanning microscopy

(2018) Elisabete C. Costa et al.   OPTICS AND LASER TECHNOLOGY

Impact of physical confinement on nuclei geometry and cell division dynamics in 3D spheroids

(2018) Annaïck Desmaison et al.   Scientific Reports

Three Dimensional Mixed-Cell Spheroids Mimic Stroma-Mediated Chemoresistance and Invasive Migration in hepatocellular carcinoma

(2018) Iftikhar Ali Khawar et al.   NEOPLASIA

Multicellular spheroid based on a triple co-culture: A novel 3D model to mimic pancreatic tumor complexity

(2018) Gianpiero Lazzari et al.   Acta Biomaterialia

A beginner’s guide to tissue clearing

(2017) Pablo Ariel   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

Advances and perspectives in tissue clearing using CLARITY

(2017) Kristian H. Reveles Jensen et al.   JOURNAL OF CHEMICAL NEUROANATOMY

Quantitative three-dimensional evaluation of immunofluorescence staining for large whole mount spheroids with light sheet microscopy

(2017) I. Smyrek et al.   Biomedical Optics Express

A tunable refractive index matching medium for live imaging cells, tissues and model organisms

(2017) Tobias Boothe et al.   eLife

Multiscale image analysis reveals structural heterogeneity of the cell microenvironment in homotypic spheroids

(2017) Alexander Schmitz et al.   Scientific Reports

Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging

(2017) Qinnan Zhang et al.   Scientific Reports

Chemical Principles in Tissue Clearing and Staining Protocols for Whole-Body Cell Profiling

(2016) Kazuki Tainaka et al.   Annual Review of Cell and Developmental Biology

On-chip clearing of arrays of 3-D cell cultures and micro-tissues

(2016) S. M. Grist et al.   Biomicrofluidics

3D tumor spheroids: an overview on the tools and techniques used for their analysis

(2016) Elisabete C. Costa et al.   BIOTECHNOLOGY ADVANCES

Deep tissue imaging: a review from a preclinical cancer research perspective

(2016) Annette Feuchtinger et al.   HISTOCHEMISTRY AND CELL BIOLOGY

Clearing of fixed tissue: a review from a microscopist’s perspective

(2016) Ludovico Silvestri et al.   JOURNAL OF BIOMEDICAL OPTICS

Selective plane illumination microscopy on a chip

(2016) Petra Paiè et al.   LAB ON A CHIP

Clearing and Labeling Techniques for Large-Scale Biological Tissues

(2016) Jinyoung Seo et al.   MOLECULES AND CELLS

Shrinkage-mediated imaging of entire organs and organisms using uDISCO

(2016) Chenchen Pan et al.   NATURE METHODS

Seeing through Musculoskeletal Tissues: Improving In Situ Imaging of Bone and the Lacunar Canalicular System through Optical Clearing

(2016) Ian M. Berke et al.   PLoS One

Clarifying intact 3D tissues on a microfluidic chip for high-throughput structural analysis

(2016) Yih Yang Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A survey of clearing techniques for 3D imaging of tissues with special reference to connective tissue

(2016) Adriano Azaripour et al.   PROGRESS IN HISTOCHEMISTRY AND CYTOCHEMISTRY

Multicellular spheroids from normal and neoplastic thyroid tissues as a suitable model to test the effects of multikinase inhibitors

(2016) Valentina Cirello et al.   Oncotarget

Engineered Breast Cancer Cell Spheroids Reproduce Biologic Properties of Solid Tumors

(2016) Stephanie L. Ham et al.   Advanced Healthcare Materials

Into the depths: Techniques for in vitro three-dimensional microtissue visualization

(2015) Pranita K. Kabadi et al.   BIOTECHNIQUES

Clarifying Tissue Clearing

(2015) Douglas S. Richardson et al.   CELL

Monitoring of Apoptosis in 3D Cell Cultures by FRET and Light Sheet Fluorescence Microscopy

(2015) Petra Weber et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Optical properties of mouse brain tissue after optical clearing with FocusClear™

(2015) Austin J. Moy et al.   JOURNAL OF BIOMEDICAL OPTICS

Understanding liver immunology using intravital microscopy

(2015) Pedro Elias Marques et al.   JOURNAL OF HEPATOLOGY

3D liver models on a microplatform: well-defined culture, engineering of liver tissue and liver-on-a-chip

(2015) Da Yoon No et al.   LAB ON A CHIP

ScaleS: an optical clearing palette for biological imaging

(2015) Hiroshi Hama et al.   NATURE NEUROSCIENCE

Optical clearing in collagen- and proteoglycan-rich osteochondral tissues

(2015) C.P. Neu et al.   OSTEOARTHRITIS AND CARTILAGE

Application and Assessment of Optical Clearing Methods for Imaging of Tissue-Engineered Neural Stem Cell Spheres

(2015) Molly E. Boutin et al.   TISSUE ENGINEERING PART C-METHODS

Significant correlation between refractive index and activity of mitochondria: single mitochondrion study

(2015) Keisuke Haseda et al.   Biomedical Optics Express

A versatile clearing agent for multi-modal brain imaging

(2015) Irene Costantini et al.   Scientific Reports

High-resolution in-depth imaging of optically cleared thick samples using an adaptive SPIM

(2015) Aurore Masson et al.   Scientific Reports

Optical Clearing in Dense Connective Tissues to Visualize Cellular Connectivity In Situ

(2015) Sarah Calve et al.   PLoS One

A Rapid Optical Clearing Protocol Using 2,2′-Thiodiethanol for Microscopic Observation of Fixed Mouse Brain

(2015) Yuka Aoyagi et al.   PLoS One

Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors

(2014) Rasheena Edmondson et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Whole-Brain Imaging with Single-Cell Resolution Using Chemical Cocktails and Computational Analysis

(2014) Etsuo A. Susaki et al.   CELL

Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing

(2014) Bin Yang et al.   CELL

iDISCO: A Simple, Rapid Method to Immunolabel Large Tissue Samples for Volume Imaging

(2014) Nicolas Renier et al.   CELL

Whole-Body Imaging with Single-Cell Resolution by Tissue Decolorization

(2014) Kazuki Tainaka et al.   CELL

3D high-content screening for the identification of compounds that target cells in dormant tumor spheroid regions

(2014) Carsten Wenzel et al.   EXPERIMENTAL CELL RESEARCH

Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy

(2014) Emilio J. Gualda et al.   Frontiers in Cellular Neuroscience

High-resolution deep imaging of live cellular spheroids with light-sheet-based fluorescence microscopy

(2013) Francesco Pampaloni et al.   CELL AND TISSUE RESEARCH

Optical Image-Guided Cancer Surgery: Challenges and Limitations

(2013) S. Keereweer et al.   CLINICAL CANCER RESEARCH

ClearT: a detergent- and solvent-free clearing method for neuronal and non-neuronal tissue

(2013) T. Kuwajima et al.   DEVELOPMENT

Recent progress in tissue optical clearing

(2013) Dan Zhu et al.   Laser & Photonics Reviews

Structural and molecular interrogation of intact biological systems

(2013) Kwanghun Chung et al.   NATURE

SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction

(2013) Meng-Tsen Ke et al.   NATURE NEUROSCIENCE

Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues

(2013) Yun Chen et al.   TheScientificWorldJOURNAL

Advances in the formation, use and understanding of multi-cellular spheroids

(2012) Toni-Marie Achilli et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Three-dimensional imaging of solvent-cleared organs using 3DISCO

(2012) Ali Ertürk et al.   Nature Protocols

Chemical Clearing and Dehydration of GFP Expressing Mouse Brains

(2012) Klaus Becker et al.   PLoS One

Light Sheet Fluorescence Microscopy

(2011) Peter A. Santi   JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY

Self-formation of functional adenohypophysis in three-dimensional culture

(2011) Hidetaka Suga et al.   NATURE

Self-organizing optic-cup morphogenesis in three-dimensional culture

(2011) Mototsugu Eiraku et al.   NATURE

Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain

(2011) Hiroshi Hama et al.   NATURE NEUROSCIENCE

Generation and differentiation of microtissues from multipotent precursor cells for use in tissue engineering

(2011) Fabian Langenbach et al.   Nature Protocols

Molecular mechanism of polyethylene glycol mediated stabilization of protein

(2010) Sanjay Rawat et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Tissue optical immersion clearing

(2010) Elina A Genina et al.   Expert Review of Medical Devices

Microtome-Free 3-Dimensional Confocal Imaging Method for Visualization of Mouse Intestine With Subcellular-Level Resolution

(2009) Ya–Yuan Fu et al.   GASTROENTEROLOGY

Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals

(2008) Mototsugu Eiraku et al.   Cell Stem Cell

On the mechanism of organelle degradation in the vertebrate lens

(2008) Steven Bassnett   EXPERIMENTAL EYE RESEARCH

Light sheet‐based fluorescence microscopy: More dimensions, more photons, and less photodamage

(2008) Emmanuel G. Reynaud et al.   HFSP Journal

Oxyradical-induced GFP damage and loss of fluorescence

(2008) Abeer A. Alnuami et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Endochondral bone tissue engineering using embryonic stem cells

(2008) J. M. Jukes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA