3D liver models on a microplatform: well-defined culture, engineering of liver tissue and liver-on-a-chip

The application of engineered liver tissues for novel drug discovery

(2015) Christine Lin et al.   Expert Opinion on Drug Discovery

A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents

(2015) Ilka Maschmeyer et al.   LAB ON A CHIP

Immune-protected xenogeneic bioartificial livers with liver-specific microarchitecture and hydrogel-encapsulated cells

(2014) Da Yoon No et al.   BIOMATERIALS

Microchip-based engineering of super-pancreatic islets supported by adipose-derived stem cells

(2014) Yesl Jun et al.   BIOMATERIALS

Induced pluripotent stem cell-derived hepatocytes and endothelial cells in multi-component hydrogel fibers for liver tissue engineering

(2014) Chan Du et al.   BIOMATERIALS

An injectable spheroid system with genetic modification for cell transplantation therapy

(2014) Satoshi Uchida et al.   BIOMATERIALS

In vitro platforms for evaluating liver toxicity

(2014) Shyam Sundhar Bale et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Organs-on-a-chip: a new tool for drug discovery

(2014) Alessandro Polini et al.   Expert Opinion on Drug Discovery

Renal function status in liver transplant patients in the first month post-transplant is associated with progressive chronic kidney disease

(2014) Kazushige Sato et al.   HEPATOLOGY RESEARCH

Research highlights: microtechnologies for engineering the cellular environment

(2014) Peter Tseng et al.   LAB ON A CHIP

Hepatic organoids for microfluidic drug screening

(2014) Sam H. Au et al.   LAB ON A CHIP

Engineering living systems on chips: from cells to human on chips

(2014) Yoshitaka Sei et al.   Microfluidics and Nanofluidics

Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant

(2014) Takanori Takebe et al.   Nature Protocols

Maturation of Induced Pluripotent Stem Cell Derived Hepatocytes by 3D-Culture

(2014) Richard L. Gieseck III et al.   PLoS One

High Throughput Micro-Well Generation of Hepatocyte Micro-Aggregates for Tissue Engineering

(2014) Elien Gevaert et al.   PLoS One

Flow Characterization of a Spinner Flask for Induced Pluripotent Stem Cell Culture Application

(2014) Mohd-Zulhilmi Ismadi et al.   PLoS One

Cell and tissue engineering for liver disease

(2014) S. N. Bhatia et al.   Science Translational Medicine

Micropatterned Cell–Cell Interactions Enable Functional Encapsulation of Primary Hepatocytes in Hydrogel Microtissues

(2014) Cheri Y. Li et al.   TISSUE ENGINEERING PART A

One-stop microfiber spinning and fabrication of a fibrous cell-encapsulated scaffold on a single microfluidic platform

(2014) D Y Park et al.   Biofabrication

Hemodynamic flow improves rat hepatocyte morphology, function, and metabolic activity in vitro

(2013) A. Dash et al.   AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

Size-controllable networked neurospheres as a 3D neuronal tissue model for Alzheimer's disease studies

(2013) Yoon Jung Choi et al.   BIOMATERIALS

3D co-culturing model of primary pancreatic islets and hepatocytes in hybrid spheroid to overcome pancreatic cell shortage

(2013) Yesl Jun et al.   BIOMATERIALS

A microfluidic device mimicking acinar concentration gradients across the liver acinus

(2013) Ming-Cheng Shih et al.   BIOMEDICAL MICRODEVICES

Stem cells and liver engineering

(2013) Laura Ordovás et al.   BIOTECHNOLOGY ADVANCES

Physiologically relevant organs on chips

(2013) Kyungsuk Yum et al.   Biotechnology Journal

Human-on-chip for therapy development and fundamental science

(2013) Camilla Luni et al.   CURRENT OPINION IN BIOTECHNOLOGY

Three-dimensional culture and cAMP signaling promote the maturation of human pluripotent stem cell-derived hepatocytes

(2013) S. Ogawa et al.   DEVELOPMENT

Long-Term Stability of Primary Rat Hepatocytes in Micropatterned Cocultures

(2013) Okechukwu Ukairo et al.   JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY

Liver-cell patterning Lab Chip: mimicking the morphology of liver lobule tissue

(2013) Chen-Ta Ho et al.   LAB ON A CHIP

Microfluidic titer plate for stratified 3D cell culture

(2013) Sebastiaan J. Trietsch et al.   LAB ON A CHIP

Skin and hair on-a-chip: in vitro skin models versus ex vivo tissue maintenance with dynamic perfusion

(2013) Beren Ataç et al.   LAB ON A CHIP

Spheroid-based three-dimensional liver-on-a-chip to investigate hepatocyte–hepatic stellate cell interactions and flow effects

(2013) Seung-A Lee et al.   LAB ON A CHIP

Vascularized and functional human liver from an iPSC-derived organ bud transplant

(2013) Takanori Takebe et al.   NATURE

Organs on microfluidic chips: A mini review

(2013) Bo Jiang et al.   Science China-Chemistry

Controlled Multiple Growth Factor Delivery from Bone Tissue Engineering Scaffolds via Designed Affinity

(2013) Darilis Suárez-González et al.   TISSUE ENGINEERING PART A

A long-term three dimensional liver co-culture system for improved prediction of clinically relevant drug-induced hepatotoxicity

(2013) Radina Kostadinova et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Multi-cell type human liver microtissues for hepatotoxicity testing

(2012) S. Messner et al.   ARCHIVES OF TOXICOLOGY

Controlled formation of heterotypic hepatic micro-organoids in anisotropic hydrogel microfibers for long-term preservation of liver-specific functions

(2012) Masumi Yamada et al.   BIOMATERIALS

3D spheroid culture of hESC/hiPSC-derived hepatocyte-like cells for drug toxicity testing

(2012) Kazuo Takayama et al.   BIOMATERIALS

Investigation of ifosfamide nephrotoxicity induced in a liver-kidney co-culture biochip

(2012) Leila Choucha-Snouber et al.   BIOTECHNOLOGY AND BIOENGINEERING

A thin-walled polydimethylsiloxane bioreactor for high-density hepatocyte sandwich culture

(2012) Guo-Dong Sean Tan et al.   BIOTECHNOLOGY AND BIOENGINEERING

Human-Scale Whole-Organ Bioengineering for Liver Transplantation: A Regenerative Medicine Approach

(2012) Hiroshi Yagi et al.   CELL TRANSPLANTATION

Advances in the formation, use and understanding of multi-cellular spheroids

(2012) Toni-Marie Achilli et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Functional 3D Human Primary Hepatocyte Spheroids Made by Co-Culturing Hepatocytes from Partial Hepatectomy Specimens and Human Adipose-Derived Stem Cells

(2012) Da Yoon No et al.   PLoS One

Rapid Generation of Multiplexed Cell Cocultures Using Acoustic Droplet Ejection Followed by Aqueous Two-Phase Exclusion Patterning

(2012) Yu Fang et al.   TISSUE ENGINEERING PART C-METHODS

Metabolomics-on-a-Chip of Hepatotoxicity Induced by Anticancer Drug Flutamide and Its Active Metabolite Hydroxyflutamide Using HepG2/C3a Microfluidic Biochips

(2012) Leila Choucha Snouber et al.   TOXICOLOGICAL SCIENCES

Liver tissue engineering based on aggregate assembly: efficient formation of endothelialized rat hepatocyte aggregates and their immobilization with biodegradable fibres

(2012) Y Pang et al.   Biofabrication

Cellular Hydrogel Biopaper for Patterned 3D Cell Culture and Modular Tissue Reconstruction

(2012) Wonhye Lee et al.   Advanced Healthcare Materials

Concave microwell based size-controllable hepatosphere as a three-dimensional liver tissue model

(2011) Sau Fung Wong et al.   BIOMATERIALS

In situ formation and collagen-alginate composite encapsulation of pancreatic islet spheroids

(2011) Bo Ram Lee et al.   BIOMATERIALS

Microfluidic wet spinning of chitosan-alginate microfibers and encapsulation of HepG2 cells in fibers

(2011) Bo Ram Lee et al.   Biomicrofluidics

Generation of primary hepatocyte microarrays by piezoelectric printing

(2011) Alicja Zarowna-Dabrowska et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

Human liver cell spheroids in extended perfusion bioreactor culture for repeated-dose drug testing

(2011) Rui M. Tostões et al.   HEPATOLOGY

Breast on-a-chip: mimicry of the channeling system of the breast for development of theranostics

(2011) Meggie M.G. Grafton et al.   Integrative Biology

A cocktail of metabolic probes demonstrates the relevance of primary human hepatocyte cultures in a microfluidic biochip for pharmaceutical drug screening

(2011) Jean-Matthieu Prot et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Diffusion-mediated in situ alginate encapsulation of cell spheroids using microscale concave well and nanoporous membrane

(2011) Kwang Ho Lee et al.   LAB ON A CHIP

Digitally tunable physicochemical coding of material composition and topography in continuous microfibres

(2011) Edward Kang et al.   NATURE MATERIALS

A Whole-Organ Regenerative Medicine Approach for Liver Replacement

(2011) Alejandro Soto-Gutierrez et al.   TISSUE ENGINEERING PART C-METHODS

From 3D cell culture to organs-on-chips

(2011) Dongeun Huh et al.   TRENDS IN CELL BIOLOGY

Cultivating hepatocytes on printed arrays of HGF and BMP7 to characterize protective effects of these growth factors during in vitro alcohol injury

(2010) Caroline N. Jones et al.   BIOMATERIALS

Heparin-based hydrogel as a matrix for encapsulation and cultivation of primary hepatocytes

(2010) Mihye Kim et al.   BIOMATERIALS

Controlled-size embryoid body formation in concave microwell arrays

(2010) Yoon Young Choi et al.   BIOMATERIALS

A robust high-throughput sandwich cell-based drug screening platform

(2010) Shufang Zhang et al.   BIOMATERIALS

The use of whole organ decellularization for the generation of a vascularized liver organoid

(2010) Pedro M. Baptista et al.   HEPATOLOGY

Preparation of arrays of cell spheroids and spheroid-monolayer cocultures within a microfluidic device

(2010) Tomoaki Okuyama et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix

(2010) Basak E Uygun et al.   NATURE MEDICINE

Breast Cancer Diagnosis Using a Microfluidic Multiplexed Immunohistochemistry Platform

(2010) Minseok S. Kim et al.   PLoS One

Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties

(2010) T. J. Bartosh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Isolation of circulating tumor cells using a microvortex-generating herringbone-chip

(2010) S. L. Stott et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reconstituting Organ-Level Lung Functions on a Chip

(2010) D. Huh et al.   SCIENCE

Controlled Growth Factor Delivery for Tissue Engineering

(2009) Prakriti Tayalia et al.   ADVANCED MATERIALS

A microfluidic hepatic coculture platform for cell-based drug metabolism studies

(2009) Eric Novik et al.   BIOCHEMICAL PHARMACOLOGY

In vitro evaluation of a multi-layer radial-flow bioreactor based on galactosylated chitosan nanofiber scaffolds

(2009) Xue-Hui Chu et al.   BIOMATERIALS

Characterization of a gastrointestinal tract microscale cell culture analog used to predict drug toxicity

(2009) Gretchen J. Mahler et al.   BIOTECHNOLOGY AND BIOENGINEERING

Improved Survival of Fulminant Liver Failure by Transplantation of Microencapsulated Cryopreserved Porcine Hepatocytes in Mice

(2009) Jie Mei et al.   CELL TRANSPLANTATION

Serum-free collagen sandwich cultures of adult rat hepatocytes maintain liver-like properties long term: A valuable model for in vitro toxicity and drug–drug interaction studies

(2009) Gregor Tuschl et al.   CHEMICO-BIOLOGICAL INTERACTIONS

Transport-mediated angiogenesis in 3D epithelial coculture

(2009) Ryo Sudo et al.   FASEB JOURNAL

Evaluation of a bioreactor with stacked sheet shaped organoids of primary hepatocytes

(2009) Tomoaki Kusumi et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Three-dimensional cell culture array using magnetic force-based cell patterning for analysis of invasive capacity of BALB/3T3/v-src

(2009) Mina Okochi et al.   LAB ON A CHIP

A microfluidic 3D hepatocyte chip for drug toxicity testing

(2009) Yi-Chin Toh et al.   LAB ON A CHIP

Perfused multiwell plate for 3D liver tissue engineering

(2009) Karel Domansky et al.   LAB ON A CHIP

Complexity in biomaterials for tissue engineering

(2009) Elsie S. Place et al.   NATURE MATERIALS

Application of magnetic force-based cell patterning for controlling cell-cell interactions in angiogenesis

(2008) Kosuke Ino et al.   BIOTECHNOLOGY AND BIOENGINEERING

Recent advances in three-dimensional multicellular spheroid culture for biomedical research

(2008) Ruei-Zhen Lin et al.   Biotechnology Journal

T-cadherin modulates hepatocyte functions in vitro

(2008) Salman R. Khetani et al.   FASEB JOURNAL

Cell microencapsulation technology: Towards clinical application

(2008) Ainhoa Murua et al.   JOURNAL OF CONTROLLED RELEASE