Seeing through Musculoskeletal Tissues: Improving In Situ Imaging of Bone and the Lacunar Canalicular System through Optical Clearing

Quantifying the osteocyte network in the human skeleton

(2015) Pascal R. Buenzli et al.   BONE

Optical clearing in collagen- and proteoglycan-rich osteochondral tissues

(2015) C.P. Neu et al.   OSTEOARTHRITIS AND CARTILAGE

A versatile clearing agent for multi-modal brain imaging

(2015) Irene Costantini et al.   Scientific Reports

Optical Clearing in Dense Connective Tissues to Visualize Cellular Connectivity In Situ

(2015) Sarah Calve et al.   PLoS One

Age-dependent regulation of tendon crimp structure, cell length and gap width with strain

(2014) Kirsten Legerlotz et al.   Acta Biomaterialia

The effect of compressive loading magnitude on in situ chondrocyte calcium signaling

(2014) Ryan M. J. Madden et al.   Biomechanics and Modeling in Mechanobiology

Imaging and quantifying solute transport across periosteum: Implications for muscle–bone crosstalk

(2014) Xiaohan Lai et al.   BONE

iDISCO: A Simple, Rapid Method to Immunolabel Large Tissue Samples for Volume Imaging

(2014) Nicolas Renier et al.   CELL

The effects of tensile-compressive loading mode and microarchitecture on microdamage in human vertebral cancellous bone

(2014) Floor M. Lambers et al.   JOURNAL OF BIOMECHANICS

Microscopy: seeing through tissue

(2014) Vivien Marx   NATURE METHODS

Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution

(2014) Bi-Chang Chen et al.   SCIENCE

Studying osteocytes within their environment

(2013) Duncan J. Webster et al.   BONE

ClearT: a detergent- and solvent-free clearing method for neuronal and non-neuronal tissue

(2013) T. Kuwajima et al.   DEVELOPMENT

In situ intracellular calcium oscillations in osteocytes in intact mouse long bones under dynamic mechanical loading

(2013) Da Jing et al.   FASEB JOURNAL

Perlecan-Containing Pericellular Matrix Regulates Solute Transport and Mechanosensing Within the Osteocyte Lacunar-Canalicular System

(2013) Bin Wang et al.   JOURNAL OF BONE AND MINERAL RESEARCH

Recent progress in tissue optical clearing

(2013) Dan Zhu et al.   Laser & Photonics Reviews

Structural and molecular interrogation of intact biological systems

(2013) Kwanghun Chung et al.   NATURE

SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction

(2013) Meng-Tsen Ke et al.   NATURE NEUROSCIENCE

Optical properties of biological tissues: a review

(2013) Steven L Jacques   PHYSICS IN MEDICINE AND BIOLOGY

In situ imaging of the autonomous intracellular Ca2+ oscillations of osteoblasts and osteocytes in bone

(2012) Yoshihito Ishihara et al.   BONE

Alterations in the osteocyte lacunar–canalicular microenvironment due to estrogen deficiency

(2012) Divya Sharma et al.   BONE

Longitudinal in vivo imaging of bone formation and resorption using fluorescence molecular tomography

(2012) F.M. Lambers et al.   BONE

Ex vivo real-time observation of Ca2+ signaling in living bone in response to shear stress applied on the bone surface

(2012) Yoshihito Ishihara et al.   BONE

Lineage Tracing

(2012) Kai Kretzschmar et al.   CELL

Quantifying load-induced solute transport and solute-matrix interaction within the osteocyte lacunar-canalicular system

(2012) Bin Wang et al.   JOURNAL OF BONE AND MINERAL RESEARCH

Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues

(2012) Brendon M. Baker et al.   JOURNAL OF CELL SCIENCE

Osteoclasts promote the formation of hematopoietic stem cell niches in the bone marrow

(2012) Anna Mansour et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Systemic Circulation and Bone Recruitment of Osteoclast Precursors Tracked by Using Fluorescent Imaging Techniques

(2012) M. Kotani et al.   JOURNAL OF IMMUNOLOGY

Utilizing confocal microscopy to measure refractive index of articular cartilage

(2012) K. WANG et al.   JOURNAL OF MICROSCOPY

An innovative transparent cranial window based on skull optical clearing

(2012) J Wang et al.   LASER PHYSICS LETTERS

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Three-dimensional imaging of solvent-cleared organs using 3DISCO

(2012) Ali Ertürk et al.   Nature Protocols

Chemical Clearing and Dehydration of GFP Expressing Mouse Brains

(2012) Klaus Becker et al.   PLoS One

Serial FIB/SEM imaging for quantitative 3D assessment of the osteocyte lacuno-canalicular network

(2011) Philipp Schneider et al.   BONE

Elevated cross-talk between subchondral bone and cartilage in osteoarthritic joints

(2011) Jun Pan et al.   BONE

Osteoclasts in bone modeling, as revealed by in vivo imaging, are essential for organogenesis in fish

(2011) Masahiro Chatani et al.   DEVELOPMENTAL BIOLOGY

Bone Regenerates via Dedifferentiation of Osteoblasts in the Zebrafish Fin

(2011) Franziska Knopf et al.   DEVELOPMENTAL CELL

Three-dimensional dynamic bone histomorphometry

(2011) Craig R Slyfield et al.   JOURNAL OF BONE AND MINERAL RESEARCH

Light Sheet Fluorescence Microscopy

(2011) Peter A. Santi   JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY

Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain

(2011) Hiroshi Hama et al.   NATURE NEUROSCIENCE

Deeper Tissue Imaging with Total Detection

(2011) E. Gratton   SCIENCE

Genetic variation in the structural pattern of osteoclast activity during post-natal growth of mouse femora

(2010) L.C. Gerstenfeld et al.   BONE

Tissue optical immersion clearing

(2010) Elina A Genina et al.   Expert Review of Medical Devices

Molecular basis for optical clearing of collagenous tissues

(2010) Jason M. Hirshburg et al.   JOURNAL OF BIOMEDICAL OPTICS

Bone histomorphometry: Standardization of nomenclature, symbols, and units: Report of the asbmr histomorphometry nomenclature committee

(2010) A. Michael Parfitt et al.   JOURNAL OF BONE AND MINERAL RESEARCH

Real-time measurement of solute transport within the lacunar-canalicular system of mechanically loaded bone: Direct evidence for load-induced fluid flow

(2010) Christopher Price et al.   JOURNAL OF BONE AND MINERAL RESEARCH

Three-dimensional in situ zonal morphology of viable growth plate chondrocytes: A confocal microscopy study

(2010) Samira Amini et al.   JOURNAL OF ORTHOPAEDIC RESEARCH

Early response to tendon fatigue damage accumulation in a novel in vivo model

(2009) David T. Fung et al.   JOURNAL OF BIOMECHANICS

In situ measurement of transport between subchondral bone and articular cartilage

(2009) Jun Pan et al.   JOURNAL OF ORTHOPAEDIC RESEARCH

The most transparent research

(2009) Melinda Wenner   NATURE MEDICINE

Modeling Fluorescence Recovery After Photobleaching in Loaded Bone: Potential Applications in Measuring Fluid and Solute Transport in the Osteocytic Lacunar-Canalicular System

(2008) Xiaozhou Zhou et al.   ANNALS OF BIOMEDICAL ENGINEERING

The alteration of a mechanical property of bone cells during the process of changing from osteoblasts to osteocytes

(2008) Yasuyo Sugawara et al.   BONE

Quantitative second harmonic generation imaging of cartilage damage

(2008) Kelvin G. M. Brockbank et al.   CELL AND TISSUE BANKING

Round versus flat: Bone cell morphology, elasticity, and mechanosensing

(2008) Rommel G. Bacabac et al.   JOURNAL OF BIOMECHANICS

Morphology of the bovine chondrocyte and of its cytoskeleton in isolation and in situ: are chondrocytes ubiquitously paired through the entire layer of articular cartilage?

(2008) Y. Sasazaki et al.   RHEUMATOLOGY