Genome Editing of Pigs for Agriculture and Biomedicine

CD163 knockout pigs are fully resistant to highly pathogenic porcine reproductive and respiratory syndrome virus

(2018) Huaqiang Yang et al.   ANTIVIRAL RESEARCH

Bempedoic Acid Lowers Low-Density Lipoprotein Cholesterol and Attenuates Atherosclerosis in Low-Density Lipoprotein Receptor–Deficient (LDLR+/−andLDLR−/−) Yucatan Miniature PigsHighlights

(2018) Amy C. Burke et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

An FBXO40 knockout generated by CRISPR/Cas9 causes muscle hypertrophy in pigs without detectable pathological effects

(2018) Yunlong Zou et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

A Huntingtin Knockin Pig Model Recapitulates Features of Selective Neurodegeneration in Huntington’s Disease

(2018) Sen Yan et al.   CELL

Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems

(2018) Han Wu et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Genetically engineered pigs as models for human disease

(2018) Carolin Perleberg et al.   Disease Models & Mechanisms

B4GALNT2 and xenotransplantation: A newly appreciated xenogeneic antigen

(2018) Guerard Byrne et al.   XENOTRANSPLANTATION

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing

(2017) Kepin Wang et al.   GENOME RESEARCH

Production of α1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and handmade cloning

(2017) Hanchao GAO et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Efficient generation of P53 biallelic knockout Diannan miniature pigs via TALENs and somatic cell nuclear transfer

(2017) Youfeng Shen et al.   Journal of Translational Medicine

Genetically Modified Pigs as Organ Donors for Xenotransplantation

(2017) Magdalena Hryhorowicz et al.   MOLECULAR BIOTECHNOLOGY

Genome-editing technologies to improve research, reproduction, and production in pigs

(2017) Kevin D. Wells et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

Reconstitution ofUCP1using CRISPR/Cas9 in the white adipose tissue of pigs decreases fat deposition and improves thermogenic capacity

(2017) Qiantao Zheng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9

(2017) Dong Niu et al.   SCIENCE

Genome editing in livestock: Are we ready for a revolution in animal breeding industry?

(2017) Jinxue Ruan et al.   TRANSGENIC RESEARCH

CRISPR/Cas9-mediated knockout of myostatin in Chinese indigenous Erhualian pigs

(2017) Kankan Wang et al.   TRANSGENIC RESEARCH

Transgenic expression of human leukocyte antigen-E attenuates GalKO.hCD46 porcine lung xenograft injury

(2017) Christopher T. Laird et al.   XENOTRANSPLANTATION

CRISPR/Cas9-mediated ApoE-/- and LDLR-/- double gene knockout in pigs elevates serum LDL-C and TC levels

(2017) Lei Huang et al.   Oncotarget

Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene

(2017) Ki-Eun Park et al.   Scientific Reports

Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function

(2017) Christine Burkard et al.   PLoS Pathogens

CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

(2017) Jun Wu et al.   Scientific Reports

Generation of GGTA1 Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors

(2016) Chin-Kai Chuang et al.   ANIMAL BIOTECHNOLOGY

Origins of Programmable Nucleases for Genome Engineering

(2016) Srinivasan Chandrasegaran et al.   JOURNAL OF MOLECULAR BIOLOGY

Replacement of Porcine CD163 Scavenger Receptor Cysteine-Rich Domain 5 with a CD163-Like Homolog Confers Resistance of Pigs to Genotype 1 but Not Genotype 2 Porcine Reproductive and Respiratory Syndrome Virus

(2016) Kevin D. Wells et al.   JOURNAL OF VIROLOGY

Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity

(2016) Josh Tycko et al.   MOLECULAR CELL

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Generation ofRUNX3knockout pigs using CRISPR/Cas9-mediated gene targeting

(2016) J-T Kang et al.   REPRODUCTION IN DOMESTIC ANIMALS

Generation of human organs in pigs via interspecies blastocyst complementation

(2016) J Wu et al.   REPRODUCTION IN DOMESTIC ANIMALS

Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

(2016) K. Nishida et al.   SCIENCE

Zygote injection of CRISPR/Cas9 RNA successfully modifies the target gene without delaying blastocyst development or altering the sex ratio in pigs

(2016) Kristin M. Whitworth et al.   TRANSGENIC RESEARCH

Efficient production of biallelicGGTA1knockout pigsbycytoplasmic microinjection of CRISPR/Cas9 into zygotes

(2016) Bjoern Petersen et al.   XENOTRANSPLANTATION

Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP

(2016) Yanzhen Bi et al.   Scientific Reports

Highly efficient generation of GGTA1 knockout pigs using a combination of TALEN mRNA and magnetic beads with somatic cell nuclear transfer

(2016) Chong FENG et al.   Journal of Integrative Agriculture

Efficient production of multi-modified pigs for xenotransplantation by ‘combineering’, gene stacking and gene editing

(2016) Konrad Fischer et al.   Scientific Reports

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Efficient Generation of Gene-Modified Pigs Harboring Precise Orthologous Human Mutation via CRISPR/Cas9-Induced Homology-Directed Repair in Zygotes

(2015) Xiaoyang Zhou et al.   HUMAN MUTATION

Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting

(2015) Fengjiao Chen et al.   Journal of Genetics and Genomics

Generation of α1,3-galactosyltransferase and cytidine monophospho-N-acetylneuraminic acid hydroxylase gene double-knockout pigs

(2015) Shuji MIYAGAWA et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets

(2015) Shengbin Rao et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

Gene-edited pigs are protected from porcine reproductive and respiratory syndrome virus

(2015) Kristin M Whitworth et al.   NATURE BIOTECHNOLOGY

DNA-binding-domain fusions enhance the targeting range and precision of Cas9

(2015) Mehmet Fatih Bolukbasi et al.   NATURE METHODS

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Genome-wide inactivation of porcine endogenous retroviruses (PERVs)

(2015) L. Yang et al.   SCIENCE

Evaluation of human and non-human primate antibody binding to pig cells lacking GGTA1/CMAH/β4GalNT2 genes

(2015) Jose L. Estrada et al.   XENOTRANSPLANTATION

Recent investigations into pig antigen and anti-pig antibody expression

(2015) Guerard W. Byrne et al.   International Journal of Surgery

Targeted mutations in myostatin by zinc-finger nucleases result in double-muscled phenotype in Meishan pigs

(2015) Lili Qian et al.   Scientific Reports

Production of Human Albumin in Pigs Through CRISPR/Cas9-Mediated Knockin of Human cDNA into Swine Albumin Locus in the Zygotes

(2015) Jin Peng et al.   Scientific Reports

Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System

(2015) Kankan Wang et al.   Scientific Reports

Genetically Engineered Livestock: Ethical Use for Food and Medical Models

(2015) Lydia C. Garas et al.   Annual Review of Animal Biosciences

When pigs fly, UCP1 makes heat

(2015) Martin Jastroch et al.   Molecular Metabolism

Use of the CRISPR/Cas9 System to Produce Genetically Engineered Pigs from In Vitro-Derived Oocytes and Embryos1

(2014) Kristin M. Whitworth et al.   BIOLOGY OF REPRODUCTION

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing

(2014) Xiaoping Li et al.   CELL RESEARCH

Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer

(2014) Xiaoqing Zhou et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

RAG1/2 Knockout Pigs with Severe Combined Immunodeficiency

(2014) J. Huang et al.   JOURNAL OF IMMUNOLOGY

Targeted Disruption of LDLR Causes Hypercholesterolemia and Atherosclerosis in Yucatan Miniature Pigs

(2014) Bryan T. Davis et al.   PLoS One

Engraftment of human iPS cells and allogeneic porcine cells into pigs with inactivated RAG2 and accompanying severe combined immunodeficiency

(2014) K. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generation of GGTA1 biallelic knockout pigs via zinc-finger nucleases and somatic cell nuclear transfer

(2014) Lei Bao et al.   Science China-Life Sciences

Efficient generation of genetically distinct pigs in a single pregnancy using multiplexed single-guide RNA and carbohydrate selection

(2014) Ping Li et al.   XENOTRANSPLANTATION

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

An Intact Sialoadhesin (Sn/SIGLEC1/CD169) Is Not Required for Attachment/Internalization of the Porcine Reproductive and Respiratory Syndrome Virus

(2013) R. S. Prather et al.   JOURNAL OF VIROLOGY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Small and large animal models in cardiac contraction research: Advantages and disadvantages

(2013) Nima Milani-Nejad et al.   PHARMACOLOGY & THERAPEUTICS

Highly Efficient Generation of GGTA1 Biallelic Knockout Inbred Mini-Pigs with TALENs

(2013) Jige Xin et al.   PLoS One

Generation of Interleukin-2 Receptor Gamma Gene Knockout Pigs from Somatic Cells Genetically Modified by Zinc Finger Nuclease-Encoding mRNA

(2013) Masahito Watanabe et al.   PLoS One

Blastocyst complementation generates exogenic pancreas in vivo in apancreatic cloned pigs

(2013) H. Matsunari et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient nonmeiotic allele introgression in livestock using custom endonucleases

(2013) W. Tan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Double knockout pigs deficient in N-glycolylneuraminic acid and Galactose α-1,3-Galactose reduce the humoral barrier to xenotransplantation

(2013) Andrew J. Lutz et al.   XENOTRANSPLANTATION

Live pigs produced from genome edited zygotes

(2013) Simon G. Lillico et al.   Scientific Reports

Il2rg Gene-Targeted Severe Combined Immunodeficiency Pigs

(2012) Shunichi Suzuki et al.   Cell Stem Cell

RNA-guided genetic silencing systems in bacteria and archaea

(2012) Blake Wiedenheft et al.   NATURE

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient TALEN-mediated gene knockout in livestock

(2012) D. F. Carlson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Double Muscling in Cattle: Genes, Husbandry, Carcasses and Meat

(2012) Leo O. Fiems   Animals

Generation of PPARγ mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning

(2011) Dongshan Yang et al.   CELL RESEARCH

Sequence specificity is obtained from the majority of modular C2H2 zinc-finger arrays

(2011) Kathy N. Lam et al.   NUCLEIC ACIDS RESEARCH

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

(2011) Tomas Cermak et al.   NUCLEIC ACIDS RESEARCH

Assembly of custom TALE-type DNA binding domains by modular cloning

(2011) Robert Morbitzer et al.   NUCLEIC ACIDS RESEARCH

Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes

(2011) Ting Li et al.   NUCLEIC ACIDS RESEARCH

Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases

(2011) J. Hauschild et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Discovery of Zinc Fingers and Their Applications in Gene Regulation and Genome Manipulation

(2010) Aaron Klug   Annual Review of Biochemistry

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS

A TALE nuclease architecture for efficient genome editing

(2010) Jeffrey C Miller et al.   NATURE BIOTECHNOLOGY

Genome editing with engineered zinc finger nucleases

(2010) Fyodor D. Urnov et al.   NATURE REVIEWS GENETICS

Parkinson's disease: from monogenic forms to genetic susceptibility factors

(2009) S. Lesage et al.   HUMAN MOLECULAR GENETICS

Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

(2009) J. Boch et al.   SCIENCE

A Simple Cipher Governs DNA Recognition by TAL Effectors

(2009) M. J. Moscou et al.   SCIENCE

Comparison of nonhomologous end joining and homologous recombination in human cells

(2008) Z MAO et al.   DNA REPAIR

Production of CFTR-null and CFTR-ΔF508 heterozygous pigs by adeno-associated virus–mediated gene targeting and somatic cell nuclear transfer

(2008) Christopher S. Rogers et al.   JOURNAL OF CLINICAL INVESTIGATION