Genome-editing technologies to improve research, reproduction, and production in pigs

Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene

(2017) Ki-Eun Park et al.   Scientific Reports

Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function

(2017) Christine Burkard et al.   PLoS Pathogens

Functional activity of the porcine Gnrhr2 gene promoter in testis-derived cells is partially conferred by nuclear factor-κB, specificity protein 1 and 3 (SP1/3) and overlapping early growth response 1/SP1/3 binding sites

(2016) Vanessa M. Brauer et al.   GENE

Genome editing revolutionize the creation of genetically modified pigs for modeling human diseases

(2016) Jing Yao et al.   HUMAN GENETICS

Replacement of Porcine CD163 Scavenger Receptor Cysteine-Rich Domain 5 with a CD163-Like Homolog Confers Resistance of Pigs to Genotype 1 but Not Genotype 2 Porcine Reproductive and Respiratory Syndrome Virus

(2016) Kevin D. Wells et al.   JOURNAL OF VIROLOGY

Mammalian interspecies substitution of immune modulatory alleles by genome editing

(2016) Simon G. Lillico et al.   Scientific Reports

LH-Independent Testosterone Secretion Is Mediated by the Interaction Between GNRH2 and Its Receptor Within Porcine Testes1

(2015) Amy T. Desaulniers et al.   BIOLOGY OF REPRODUCTION

Activation of the Transcription Factor Nuclear Factor-Kappa B in Uterine Luminal Epithelial Cells by Interleukin 1 Beta 2: A Novel Interleukin 1 Expressed by the Elongating Pig Conceptus1

(2015) Daniel J. Mathew et al.   BIOLOGY OF REPRODUCTION

Gene-edited pigs are protected from porcine reproductive and respiratory syndrome virus

(2015) Kristin M Whitworth et al.   NATURE BIOTECHNOLOGY

Use of the CRISPR/Cas9 System to Produce Genetically Engineered Pigs from In Vitro-Derived Oocytes and Embryos1

(2014) Kristin M. Whitworth et al.   BIOLOGY OF REPRODUCTION

One-step generation of knockout pigs by zygote injection of CRISPR/Cas system

(2014) Tang Hai et al.   CELL RESEARCH

Pig genomics for biomedicine

(2013) Randall S Prather   NATURE BIOTECHNOLOGY

Rapid Cohort Generation and Analysis of Disease Spectrum of Large Animal Model of Cone Dystrophy

(2013) Corinne Kostic et al.   PLoS One

Transgenic pig carrying green fluorescent proteasomes

(2013) E. L. Miles et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Live pigs produced from genome edited zygotes

(2013) Simon G. Lillico et al.   Scientific Reports

A Porcine Model of Familial Adenomatous Polyposis

(2012) Tatiana Flisikowska et al.   GASTROENTEROLOGY

Analyses of pig genomes provide insight into porcine demography and evolution

(2012) Martien A. M. Groenen et al.   NATURE

Efficient TALEN-mediated gene knockout in livestock

(2012) D. F. Carlson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Disruption of the Survival Motor Neuron (SMN) gene in pigs using ssDNA

(2011) Monique A. Lorson et al.   TRANSGENIC RESEARCH

Strategies for selection marker-free swine transgenesis using the Sleeping Beauty transposon system

(2011) Daniel F. Carlson et al.   TRANSGENIC RESEARCH

Gene targeting with zinc finger nucleases to produce cloned eGFP knockout pigs

(2010) Jeffrey J. Whyte et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

Oct4-Enhanced Green Fluorescent Protein Transgenic Pigs: A New Large Animal Model for Reprogramming Studies

(2010) Monika Nowak-Imialek et al.   STEM CELLS AND DEVELOPMENT

Generation of antibody- and B cell-deficient pigs by targeted disruption of the J-region gene segment of the heavy chain locus

(2010) M. Mendicino et al.   TRANSGENIC RESEARCH

Targeted disruption of the porcine immunoglobulin kappa light chain locus

(2010) J. Ramsoondar et al.   TRANSGENIC RESEARCH

Production of CFTR-null and CFTR-ΔF508 heterozygous pigs by adeno-associated virus–mediated gene targeting and somatic cell nuclear transfer

(2008) Christopher S. Rogers et al.   JOURNAL OF CLINICAL INVESTIGATION

Disruption of the CFTR Gene Produces a Model of Cystic Fibrosis in Newborn Pigs

(2008) C. S. Rogers et al.   SCIENCE